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Items: 1 to 50 of 162

1.

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications.

Zavec D, Gasser B, Mattanovich D.

Biotechnol Bioeng. 2020 Feb 8. doi: 10.1002/bit.27303. [Epub ahead of print]

PMID:
32034758
2.

A subcellular proteome atlas of the yeast Komagataella phaffii.

Valli M, Grillitsch K, Grünwald-Gruber C, Tatto NE, Hrobath B, Klug L, Ivashov V, Hauzmayer S, Koller M, Tir N, Leisch F, Gasser B, Graf AB, Altmann F, Daum G, Mattanovich D.

FEMS Yeast Res. 2020 Feb 1;20(1). pii: foaa001. doi: 10.1093/femsyr/foaa001.

3.

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2.

Gassler T, Sauer M, Gasser B, Egermeier M, Troyer C, Causon T, Hann S, Mattanovich D, Steiger MG.

Nat Biotechnol. 2020 Feb;38(2):210-216. doi: 10.1038/s41587-019-0363-0. Epub 2019 Dec 16.

PMID:
31844294
4.

The secretome of Pichia pastoris in fed-batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time.

Burgard J, Grünwald-Gruber C, Altmann F, Zanghellini J, Valli M, Mattanovich D, Gasser B.

Microb Biotechnol. 2020 Mar;13(2):479-494. doi: 10.1111/1751-7915.13499. Epub 2019 Nov 6.

5.

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris.

Marsalek L, Puxbaum V, Buchetics M, Mattanovich D, Gasser B.

Microb Cell Fact. 2019 Jul 3;18(1):119. doi: 10.1186/s12934-019-1155-4.

6.

Engineering of alcohol dehydrogenase 2 hybrid-promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol.

Ergün BG, Gasser B, Mattanovich D, Çalık P.

Biotechnol Bioeng. 2019 Oct;116(10):2674-2686. doi: 10.1002/bit.27095. Epub 2019 Jul 9.

PMID:
31237681
7.

CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.

Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R.

Methods Mol Biol. 2019;1923:211-225. doi: 10.1007/978-1-4939-9024-5_9.

PMID:
30737742
8.

Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts.

Zahrl RJ, Gasser B, Mattanovich D, Ferrer P.

Methods Mol Biol. 2019;1923:75-95. doi: 10.1007/978-1-4939-9024-5_2. Review.

PMID:
30737735
9.

Engineering of the citrate exporter protein enables high citric acid production in Aspergillus niger.

Steiger MG, Rassinger A, Mattanovich D, Sauer M.

Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13.

PMID:
30553933
10.

A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii.

Ata Ö, Rebnegger C, Tatto NE, Valli M, Mairinger T, Hann S, Steiger MG, Çalık P, Mattanovich D.

Nat Commun. 2018 Nov 21;9(1):4911. doi: 10.1038/s41467-018-07430-4.

11.

A yeast for all seasons - Is Pichia pastoris a suitable chassis organism for future bioproduction?

Gasser B, Mattanovich D.

FEMS Microbiol Lett. 2018 Sep 1;365(17). doi: 10.1093/femsle/fny181. Review.

PMID:
30052876
12.

Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris.

Prielhofer R, Reichinger M, Wagner N, Claes K, Kiziak C, Gasser B, Mattanovich D.

Biotechnol Bioeng. 2018 Oct;115(10):2479-2488. doi: 10.1002/bit.26800. Epub 2018 Aug 8.

13.

Identification and characterization of the Komagataella phaffii mating pheromone genes.

Heistinger L, Moser J, Tatto NE, Valli M, Gasser B, Mattanovich D.

FEMS Yeast Res. 2018 Aug 1;18(5). doi: 10.1093/femsyr/foy051.

14.

Metabolic engineering of Pichia pastoris.

Peña DA, Gasser B, Zanghellini J, Steiger MG, Mattanovich D.

Metab Eng. 2018 Nov;50:2-15. doi: 10.1016/j.ymben.2018.04.017. Epub 2018 Apr 25. Review.

15.

The impact of ERAD on recombinant protein secretion in Pichia pastoris (syn Komagataella spp.).

Zahrl RJ, Mattanovich D, Gasser B.

Microbiology. 2018 Apr;164(4):453-463. doi: 10.1099/mic.0.000630. Epub 2018 Mar 13.

PMID:
29533745
16.

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris.

Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H.

BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.

17.

Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation.

Heistinger L, Gasser B, Mattanovich D.

Mol Cell Biol. 2017 Dec 29;38(2). pii: e00398-17. doi: 10.1128/MCB.00398-17. Print 2018 Jan 15.

18.

Microbial organic acid production as carbon dioxide sink.

Steiger MG, Mattanovich D, Sauer M.

FEMS Microbiol Lett. 2017 Nov 15;364(21). doi: 10.1093/femsle/fnx212.

PMID:
29029230
19.

Systems biotechnology for protein production in Pichia pastoris.

Zahrl RJ, Peña DA, Mattanovich D, Gasser B.

FEMS Yeast Res. 2017 Nov 1;17(7). doi: 10.1093/femsyr/fox068. Review.

PMID:
28934418
20.

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures.

Burgard J, Valli M, Graf AB, Gasser B, Mattanovich D.

Microb Cell Fact. 2017 Jul 11;16(1):117. doi: 10.1186/s12934-017-0730-9.

21.

Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris.

Ata Ö, Prielhofer R, Gasser B, Mattanovich D, Çalık P.

Biotechnol Bioeng. 2017 Oct;114(10):2319-2327. doi: 10.1002/bit.26363. Epub 2017 Jul 27.

PMID:
28650069
22.

An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger.

Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG.

Bioresour Technol. 2017 Dec;245(Pt B):1327-1333. doi: 10.1016/j.biortech.2017.05.004. Epub 2017 May 4.

PMID:
28533066
23.

Impact of glutathione metabolism on zinc homeostasis in Saccharomyces cerevisiae.

Steiger MG, Patzschke A, Holz C, Lang C, Causon T, Hann S, Mattanovich D, Sauer M.

FEMS Yeast Res. 2017 Jun 1;17(4). doi: 10.1093/femsyr/fox028.

PMID:
28505300
24.

Non-genetic impact factors on chronological lifespan and stress resistance of baker's yeast.

Sauer M, Mattanovich D.

Microb Cell. 2016 Apr 12;3(6):232-235. doi: 10.15698/mic2016.06.504.

25.

Metabolomics of Pichia pastoris: impact of buffering conditions on the kinetics and nature of metabolite loss during quenching.

Mattanovich M, Russmayer H, Scharl-Hirsch T, Puxbaum V, Burgard J, Mattanovich D, Hann S.

FEMS Yeast Res. 2017 May 1;17(3). doi: 10.1093/femsyr/fox016.

PMID:
28334329
26.

Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.

Moser JW, Prielhofer R, Gerner SM, Graf AB, Wilson IB, Mattanovich D, Dragosits M.

Microb Cell Fact. 2017 Mar 17;16(1):49. doi: 10.1186/s12934-017-0661-5.

27.

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris.

Cámara E, Landes N, Albiol J, Gasser B, Mattanovich D, Ferrer P.

Sci Rep. 2017 Mar 15;7:44302. doi: 10.1038/srep44302.

28.

Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris.

Marsalek L, Gruber C, Altmann F, Aleschko M, Mattanovich D, Gasser B, Puxbaum V.

Biotechnol J. 2017 May;12(5). doi: 10.1002/biot.201600584. Epub 2017 Mar 30.

PMID:
28230321
29.

The vitamin-sensitive promoter PTHI11 enables pre-defined autonomous induction of recombinant protein production in Pichia pastoris.

Landes N, Gasser B, Vorauer-Uhl K, Lhota G, Mattanovich D, Maurer M.

Biotechnol Bioeng. 2016 Dec;113(12):2633-2643. doi: 10.1002/bit.26041. Epub 2016 Jul 8.

PMID:
27345605
30.

Functional inclusion bodies produced in the yeast Pichia pastoris.

Rueda F, Gasser B, Sánchez-Chardi A, Roldán M, Villegas S, Puxbaum V, Ferrer-Miralles N, Unzueta U, Vázquez E, Garcia-Fruitós E, Mattanovich D, Villaverde A.

Microb Cell Fact. 2016 Oct 1;15(1):166.

31.

Curation of the genome annotation of Pichia pastoris (Komagataella phaffii) CBS7435 from gene level to protein function.

Valli M, Tatto NE, Peymann A, Gruber C, Landes N, Ekker H, Thallinger GG, Mattanovich D, Gasser B, Graf AB.

FEMS Yeast Res. 2016 Sep;16(6). pii: fow051. doi: 10.1093/femsyr/fow051. Epub 2016 Jul 6.

PMID:
27388471
32.

The bud tip is the cellular hot spot of protein secretion in yeasts.

Puxbaum V, Gasser B, Mattanovich D.

Appl Microbiol Biotechnol. 2016 Sep;100(18):8159-68. doi: 10.1007/s00253-016-7674-6. Epub 2016 Jun 23.

33.

Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates.

Rebnegger C, Vos T, Graf AB, Valli M, Pronk JT, Daran-Lapujade P, Mattanovich D.

Appl Environ Microbiol. 2016 Jul 15;82(15):4570-4583. doi: 10.1128/AEM.00638-16. Print 2016 Aug 1.

34.

Complete genome sequence and transcriptome regulation of the pentose utilizing yeast Sugiyamaella lignohabitans.

Bellasio M, Peymann A, Steiger MG, Valli M, Sipiczki M, Sauer M, Graf AB, Marx H, Mattanovich D.

FEMS Yeast Res. 2016 Jun;16(4). pii: fow037. doi: 10.1093/femsyr/fow037. Epub 2016 Apr 17.

PMID:
27189363
35.

Corrigendum: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.

Schosserer M, Minois N, Angerer TB, Amring M, Dellago H, Harreither E, Calle-Perez A, Pircher A, Peter Gerstl M, Pfeifenberger S, Brandl C, Sonntagbauer M, Kriegner A, Linder A, Weinhäusel A, Mohr T, Steiger M, Mattanovich D, Rinnerthaler M, Karl T, Sharma S, Entian KD, Kos M, Breitenbach M, Wilson IB, Polacek N, Grillari-Voglauer R, Breitenbach-Koller L, Grillari J.

Nat Commun. 2016 May 11;7:11530. doi: 10.1038/ncomms11530. No abstract available.

36.

Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris.

Nocon J, Steiger M, Mairinger T, Hohlweg J, Rußmayer H, Hann S, Gasser B, Mattanovich D.

Appl Microbiol Biotechnol. 2016 Jul;100(13):5955-63. doi: 10.1007/s00253-016-7363-5. Epub 2016 Mar 28.

37.

Characterizing MttA as a mitochondrial cis-aconitic acid transporter by metabolic engineering.

Steiger MG, Punt PJ, Ram AFJ, Mattanovich D, Sauer M.

Metab Eng. 2016 May;35:95-104. doi: 10.1016/j.ymben.2016.02.003. Epub 2016 Feb 10.

PMID:
26875555
38.

Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.

Gasser B, Steiger MG, Mattanovich D.

Microb Cell Fact. 2015 Dec 2;14:196. doi: 10.1186/s12934-015-0387-1.

39.

Gas Chromatography-Quadrupole Time-of-Flight Mass Spectrometry-Based Determination of Isotopologue and Tandem Mass Isotopomer Fractions of Primary Metabolites for (13)C-Metabolic Flux Analysis.

Mairinger T, Steiger M, Nocon J, Mattanovich D, Koellensperger G, Hann S.

Anal Chem. 2015 Dec 1;87(23):11792-802. doi: 10.1021/acs.analchem.5b03173. Epub 2015 Nov 10.

PMID:
26513365
40.

Systems-level organization of yeast methylotrophic lifestyle.

Rußmayer H, Buchetics M, Gruber C, Valli M, Grillitsch K, Modarres G, Guerrasio R, Klavins K, Neubauer S, Drexler H, Steiger M, Troyer C, Al Chalabi A, Krebiehl G, Sonntag D, Zellnig G, Daum G, Graf AB, Altmann F, Koellensperger G, Hann S, Sauer M, Mattanovich D, Gasser B.

BMC Biol. 2015 Sep 23;13:80. doi: 10.1186/s12915-015-0186-5.

41.

Metabolomics sampling of Pichia pastoris revisited: rapid filtration prevents metabolite loss during quenching.

Russmayer H, Troyer C, Neubauer S, Steiger MG, Gasser B, Hann S, Koellensperger G, Sauer M, Mattanovich D.

FEMS Yeast Res. 2015 Sep;15(6). pii: fov049. doi: 10.1093/femsyr/fov049. Epub 2015 Jun 19.

PMID:
26091839
42.

Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications for protein localization.

Heiss S, Puxbaum V, Gruber C, Altmann F, Mattanovich D, Gasser B.

Microbiology. 2015 Jul;161(7):1356-68. doi: 10.1099/mic.0.000105. Epub 2015 May 1.

PMID:
25934645
43.

Enhanced glutathione production by evolutionary engineering of Saccharomyces cerevisiae strains.

Patzschke A, Steiger MG, Holz C, Lang C, Mattanovich D, Sauer M.

Biotechnol J. 2015 Sep;10(11):1719-26. doi: 10.1002/biot.201400809. Epub 2015 May 26.

PMID:
25914400
44.

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level.

Prielhofer R, Cartwright SP, Graf AB, Valli M, Bill RM, Mattanovich D, Gasser B.

BMC Genomics. 2015 Mar 11;16:167. doi: 10.1186/s12864-015-1393-8.

45.

Metabolomics integrated elementary flux mode analysis in large metabolic networks.

Gerstl MP, Ruckerbauer DE, Mattanovich D, Jungreuthmayer C, Zanghellini J.

Sci Rep. 2015 Mar 10;5:8930. doi: 10.1038/srep08930.

46.

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris.

Puxbaum V, Mattanovich D, Gasser B.

Appl Microbiol Biotechnol. 2015 Apr;99(7):2925-38. doi: 10.1007/s00253-015-6470-z. Epub 2015 Feb 27. Review.

PMID:
25722021
47.

Organic acids from lignocellulose: Candida lignohabitans as a new microbial cell factory.

Bellasio M, Mattanovich D, Sauer M, Marx H.

J Ind Microbiol Biotechnol. 2015 May;42(5):681-91. doi: 10.1007/s10295-015-1590-0. Epub 2015 Feb 5.

PMID:
25651876
48.

Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.

Schosserer M, Minois N, Angerer TB, Amring M, Dellago H, Harreither E, Calle-Perez A, Pircher A, Gerstl MP, Pfeifenberger S, Brandl C, Sonntagbauer M, Kriegner A, Linder A, Weinhäusel A, Mohr T, Steiger M, Mattanovich D, Rinnerthaler M, Karl T, Sharma S, Entian KD, Kos M, Breitenbach M, Wilson IB, Polacek N, Grillari-Voglauer R, Breitenbach-Koller L, Grillari J.

Nat Commun. 2015 Jan 30;6:6158. doi: 10.1038/ncomms7158. Erratum in: Nat Commun. 2016 May 11;7:11530.

49.

Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion.

Delic M, Graf AB, Koellensperger G, Haberhauer-Troyer C, Hann S, Mattanovich D, Gasser B.

Microb Cell. 2014 Oct 31;1(11):376-386. doi: 10.15698/mic2014.11.173.

50.

Pichia pastoris Aft1--a novel transcription factor, enhancing recombinant protein secretion.

Ruth C, Buchetics M, Vidimce V, Kotz D, Naschberger S, Mattanovich D, Pichler H, Gasser B.

Microb Cell Fact. 2014 Sep 3;13:120. doi: 10.1186/s12934-014-0120-5.

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