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J Clin Diagn Res. 2014 Nov;8(11):PC01-4. doi: 10.7860/JCDR/2014/8970.5104. Epub 2014 Nov 20.

Prevalence of Heptavalent Vaccine-related Pneumococcal Serotypes in Nasopharyngeal carrier in children under five years old in Shahrekord, Iran by Multiplex-PCR during 2010- 2011.

Author information

1
Pediatrics Infectious Diseases Specialist, Department of Pediatrics, Shahrekord University of Medical Sciences , Shahrekord, Iran .
2
Infectious & Tropical Diseases Specialist, Department of Infectious & Tropical Diseases, Faculty of Medicine, Shahrekord University of Medical Sciences , Shahrekord, Iran .
3
Molecular Genetic Ph.D, Biotechnology Research Centre, Islamic Azad University , Shahrekord Branch, Shahrekord, Iran .
4
General Practitioner, Department of Internal Medicine, Shahrekord University of Medical Sciences , Shahrekord, Iran .

Abstract

BACKGROUND:

Heptavalent pneumococcal vaccine which included pneumococcal serotypes 4, 6B, 9V,14, 18C, 19F and 23F has been regularly used and is effective on preventing invasive pneumococcal infection. This study aimed to determine vaccine-related serotype distribution in nasopharyngeal carrier and healthy children under five years old.

MATERIALS AND METHODS:

In this cross-sectional study from September 2010 to September 2011, 363 nasopharyngeal specimens were collected from healthy children in day care centers. In positive cultures of streptococcus pneumoniae (S. pneumonia) distribution, serotypes were detected by Multiplex polymerase chain reaction (PCR). Electrophoresis of PCR products was used for detection of serotypes of S. pneumoniae.

RESULTS:

The carrier rate of S. pneumoniae was 29.5% with 95% confidence interval as 24.8- 34.5%. Electrophoresis of PCR products for detection of serotypes of S. pneumonia revealed 430, 220, 753, 189, 573, 304, and 384 bp (s) for 4, 6B, 9V, 14, 18C, 19F, and 23F serotypes of S. pneumoniae, respectively. The frequency of 23F, 6B, 19F, and 18C serotypes were 43%, 34%, 18%, and 5% respectively, but other serotypes (4, 9V and 14) were not detected.

CONCLUSION:

Based on the 30% carrier rate and high prevalence of most of heptavalent pneumococcal conjugate vaccine serotypes in our study, this vaccine should be used for prevention of invasive infection in Iranian children.

KEYWORDS:

Electrophoresis; Heptavalent pneumococcal conjugate vaccine; PCR product; Streptococcus pneumoniae

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