Format

Send to

Choose Destination
J Cell Physiol. 2019 Dec;234(12):23763-23773. doi: 10.1002/jcp.28944. Epub 2019 Jun 7.

Olfactory mucosa stem cells: An available candidate for the treatment of the Parkinson's disease.

Author information

1
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2
Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
3
ENT and Head & Neck Research Center and Department, The Five Senses Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
4
Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran.
5
Departments of Medicine, University of Alberta, Edmonton, Alberta, Canada.

Abstract

Olfactory ectomesenchymal stem cells (OE-MSCs) possess the immunosuppressive activity and regeneration capacity and hold a lot of promises for neurodegenerative disorders treatment. This study aimed to determine OE-MSCs which are able to augment and differentiate into functional neurons and regenerate the CNS and also examine whether the implantation of OE-MSCs in the pars compacta of the substantia nigra (SNpc) can improve Parkinson's symptoms in a rat model-induced with 6-hydroxydopamine. We isolated OE-MSCs from lamina propria in olfactory mucosa and characterized them using flow cytometry and immunocytochemistry. The therapeutic potential of OE-MSCs was evaluated by the transplantation of isolated cells using a rat model of acute SN injury as a Parkinson's disease. Significant behavioral improvement in Parkinsonian rats was elicited by the OE-MSCs. The results demonstrate that the expression of PAX2, PAX5, PITX3, dopamine transporter, and tyrosine hydroxylase was increased by OE-MSCs compared to the control group which is analyzed with real-time polymerase chain reaction technique and immunohistochemical staining. In the outcome, the transplantation of 1,1'-dioctadecyl-3,3,3'3'-tetramethyl indocarbocyanine perchlorate labeled OE-MSCs that were fully differentiated to dopaminergic neurons contribute to a substantial improvement in patients with Parkinson's. Together, our results provide that using OE-MSCs in neurodegenerative disorders might lead to better neural regeneration.

KEYWORDS:

Parkinson's disease; ectomesenchymal stem cells; olfactory mucosa

PMID:
31173364
DOI:
10.1002/jcp.28944

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center