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Biochem Biophys Res Commun. 2014 Jun 6;448(3):274-80. doi: 10.1016/j.bbrc.2014.04.110. Epub 2014 Apr 30.

Dystroglycan depletion inhibits the functions of differentiated HL-60 cells.

Author information

1
Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
2
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-IPN), Mexico City, Mexico.
3
Department of Biomedical Science, University of Sheffield, Sheffield, UK.
4
Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico. Electronic address: dcereced@prodigy.net.mx.

Abstract

Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.

KEYWORDS:

Differentiation; Dystroglycan; HL-60 cells; Migration; Phagocytosis; Respiratory burst activity

PMID:
24792180
DOI:
10.1016/j.bbrc.2014.04.110
[Indexed for MEDLINE]

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