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G3 (Bethesda). 2017 Jun 7;7(6):1767-1773. doi: 10.1534/g3.117.039388.

Physical Confirmation and Comparative Genomics of the Rat Mammary carcinoma susceptibility 3 Quantitative Trait Locus.

Author information

1
Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Kentucky 40292.
2
Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Kentucky 40292 david.samuelson@louisville.edu.
3
James Graham Brown Cancer Center, University of Louisville School of Medicine, Kentucky 40202.
4
Center for Genetics and Molecular Medicine, University of Louisville School of Medicine, Kentucky 40202.

Abstract

Human breast and rat mammary cancer susceptibility are complex phenotypes where complete sets of risk associated loci remain to be identified for both species. We tested multiple congenic rat strains to physically confirm and positionally map rat Mammary carcinoma susceptibility 3 (Mcs3)-a mammary cancer resistance allele previously predicted at Rattus norvegicus chromosome 1 (RNO1). The mammary cancer susceptible Wistar Furth (WF) strain was the recipient, and the mammary cancer resistant Copenhagen (Cop) strain was the RNO1-segment donor for congenics. Inbred WF females averaged 6.3 carcinogen-induced mammary carcinomas per rat. Two WF.Cop congenic strains averaged 2.8 and 3.4 mammary carcinomas per rat, which confirmed Mcs3 as an independently acting allele. Two other WF.Cop congenic strains averaged 6.6 and 8.1 mammary carcinomas per rat, and, thus, did not contain Mcs3 Rat Mcs3 was delimited to 27.8 Mb of RNO1 from rs8149408 to rs105131702 (RNO1:143700228-171517317 of RGSC 6.0/rn6). Human genetic variants with p values for association to breast cancer risk below 10-7 had not been reported for Mcs3 orthologous loci; however, human variants located in Mcs3-orthologous regions with potential association to risk (10-7 < p < 10-3) were listed in some population-based studies. Further, rat Mcs3 contains sequence orthologous to human 11q13/14-a region frequently amplified in female breast cancer. We conclude that Mcs3 is an independently acting mammary carcinoma resistance allele. Human population-based, genome-targeted association studies interrogating Mcs3 orthologous loci may yield novel breast cancer risk associated variants and genes.

KEYWORDS:

breast cancer susceptibility; mammary carcinogenesis; rat QTL

PMID:
28391240
PMCID:
PMC5473756
DOI:
10.1534/g3.117.039388
[Indexed for MEDLINE]
Free PMC Article

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