Sort by
Items per page

Send to

Choose Destination

Search results

Items: 1 to 50 of 105


Using Single-Molecule Chemo-Mechanical Unfolding to Simultaneously Probe Multiple Structural Parameters in Protein Folding.

Guinn EJ, Marqusee S.

Methods Protoc. 2019 Apr 20;2(2). pii: E32. doi: 10.3390/mps2020032.


A small single-domain protein folds through the same pathway on and off the ribosome.

Guinn EJ, Tian P, Shin M, Best RB, Marqusee S.

Proc Natl Acad Sci U S A. 2018 Nov 27;115(48):12206-12211. doi: 10.1073/pnas.1810517115. Epub 2018 Nov 8.


Modulating long-range energetics via helix stabilization: A case study using T4 lysozyme.

Rosemond SN, Hamadani KM, Cate JHD, Marqusee S.

Protein Sci. 2018 Dec;27(12):2084-2093. doi: 10.1002/pro.3521.


Tracing a protein's folding pathway over evolutionary time using ancestral sequence reconstruction and hydrogen exchange.

Lim SA, Bolin ER, Marqusee S.

Elife. 2018 Sep 11;7. pii: e38369. doi: 10.7554/eLife.38369.


Kinetic and structural comparison of a protein's cotranslational folding and refolding pathways.

Samelson AJ, Bolin E, Costello SM, Sharma AK, O'Brien EP, Marqusee S.

Sci Adv. 2018 May 30;4(5):eaas9098. doi: 10.1126/sciadv.aas9098. eCollection 2018 May.


Editorial Overview: Single-Molecule Approaches up to Difficult Challenges in Folding and Dynamics.

Zhang Y, Ha T, Marqusee S.

J Mol Biol. 2018 Feb 16;430(4):405-408. doi: 10.1016/j.jmb.2017.12.019. Epub 2017 Dec 27. No abstract available.


The burst-phase folding intermediate of ribonuclease H changes conformation over evolutionary history.

Lim SA, Marqusee S.

Biopolymers. 2018 Aug;109(8):e23086. doi: 10.1002/bip.23086. Epub 2017 Nov 20.


Exploring the Denatured State Ensemble by Single-Molecule Chemo-Mechanical Unfolding: The Effect of Force, Temperature, and Urea.

Guinn EJ, Marqusee S.

J Mol Biol. 2018 Feb 16;430(4):450-464. doi: 10.1016/j.jmb.2017.07.022. Epub 2017 Aug 4.


An in vitro tag-and-modify protein sample generation method for single-molecule fluorescence resonance energy transfer.

Hamadani KM, Howe J, Jensen MK, Wu P, Cate JHD, Marqusee S.

J Biol Chem. 2017 Sep 22;292(38):15636-15648. doi: 10.1074/jbc.M117.791723. Epub 2017 Jul 28.


Quantitative determination of ribosome nascent chain stability.

Samelson AJ, Jensen MK, Soto RA, Cate JH, Marqusee S.

Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):13402-13407. Epub 2016 Nov 7.


Evolutionary trend toward kinetic stability in the folding trajectory of RNases H.

Lim SA, Hart KM, Harms MJ, Marqusee S.

Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13045-13050. Epub 2016 Oct 31.


The thermostability and specificity of ancient proteins.

Wheeler LC, Lim SA, Marqusee S, Harms MJ.

Curr Opin Struct Biol. 2016 Jun;38:37-43. doi: 10.1016/ Epub 2016 Jun 9. Review.


Reply to Alberti: Are in vitro folding experiments relevant in vivo?

Zhuravlev PI, Hinczewski M, Chakrabarti S, Marqusee S, Thirumalai D.

Proc Natl Acad Sci U S A. 2016 Jun 7;113(23):E3192. doi: 10.1073/pnas.1603395113. Epub 2016 May 25. No abstract available.


Force-dependent switch in protein unfolding pathways and transition-state movements.

Zhuravlev PI, Hinczewski M, Chakrabarti S, Marqusee S, Thirumalai D.

Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):E715-24. doi: 10.1073/pnas.1515730113. Epub 2016 Jan 27.


When the Scaffold Cannot Be Ignored: The Role of the Hydrophobic Core in Ligand Binding and Specificity.

Koulechova DA, Tripp KW, Horner G, Marqusee S.

J Mol Biol. 2015 Oct 9;427(20):3316-3326. doi: 10.1016/j.jmb.2015.08.014. Epub 2015 Aug 21.


Interaction with Single-stranded DNA-binding Protein Stimulates Escherichia coli Ribonuclease HI Enzymatic Activity.

Petzold C, Marceau AH, Miller KH, Marqusee S, Keck JL.

J Biol Chem. 2015 Jun 5;290(23):14626-36. doi: 10.1074/jbc.M115.655134. Epub 2015 Apr 22.


Single-molecule chemo-mechanical unfolding reveals multiple transition state barriers in a small single-domain protein.

Guinn EJ, Jagannathan B, Marqusee S.

Nat Commun. 2015 Apr 17;6:6861. doi: 10.1038/ncomms7861.


Autonomously folding protein fragments reveal differences in the energy landscapes of homologous RNases H.

Rosen LE, Marqusee S.

PLoS One. 2015 Mar 24;10(3):e0119640. doi: 10.1371/journal.pone.0119640. eCollection 2015.


Discovery of multiple hidden allosteric sites by combining Markov state models and experiments.

Bowman GR, Bolin ER, Hart KM, Maguire BC, Marqusee S.

Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):2734-9. doi: 10.1073/pnas.1417811112. Epub 2015 Feb 17.


The heat released during catalytic turnover enhances the diffusion of an enzyme.

Riedel C, Gabizon R, Wilson CA, Hamadani K, Tsekouras K, Marqusee S, Pressé S, Bustamante C.

Nature. 2015 Jan 8;517(7533):227-30. doi: 10.1038/nature14043. Epub 2014 Dec 10.


Thermodynamic system drift in protein evolution.

Hart KM, Harms MJ, Schmidt BH, Elya C, Thornton JW, Marqusee S.

PLoS Biol. 2014 Nov 11;12(11):e1001994. doi: 10.1371/journal.pbio.1001994. eCollection 2014 Nov. Erratum in: PLoS Biol. 2015 Feb;13(2):e1002091.


Non-native structure appears in microseconds during the folding of E. coli RNase H.

Rosen LE, Kathuria SV, Matthews CR, Bilsel O, Marqusee S.

J Mol Biol. 2015 Jan 30;427(2):443-53. doi: 10.1016/j.jmb.2014.10.003. Epub 2014 Oct 13.


Evidence for close side-chain packing in an early protein folding intermediate previously assumed to be a molten globule.

Rosen LE, Connell KB, Marqusee S.

Proc Natl Acad Sci U S A. 2014 Oct 14;111(41):14746-51. doi: 10.1073/pnas.1410630111. Epub 2014 Sep 25.


Single molecule conformational memory extraction: p5ab RNA hairpin.

Pressé S, Peterson J, Lee J, Elms P, MacCallum JL, Marqusee S, Bustamante C, Dill K.

J Phys Chem B. 2014 Jun 19;118(24):6597-603. doi: 10.1021/jp500611f. Epub 2014 Jun 5.


Kinetic evidence for a two-stage mechanism of protein denaturation by guanidinium chloride.

Jha SK, Marqusee S.

Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4856-61. doi: 10.1073/pnas.1315453111. Epub 2014 Mar 17.


Mono and dual cofactor dependence of human cystathionine β-synthase enzyme variants in vivo and in vitro.

Dimster-Denk D, Tripp KW, Marini NJ, Marqusee S, Rine J.

G3 (Bethesda). 2013 Oct 3;3(10):1619-28. doi: 10.1534/g3.113.006916.


Protein folding and unfolding under force.

Jagannathan B, Marqusee S.

Biopolymers. 2013 Nov;99(11):860-9. doi: 10.1002/bip.22321.


Stepwise protein folding at near amino acid resolution by hydrogen exchange and mass spectrometry.

Hu W, Walters BT, Kan ZY, Mayne L, Rosen LE, Marqusee S, Englander SW.

Proc Natl Acad Sci U S A. 2013 May 7;110(19):7684-9. doi: 10.1073/pnas.1305887110. Epub 2013 Apr 19.


Folding and binding.

Udgaonkar J, Marqusee S.

Curr Opin Struct Biol. 2013 Feb;23(1):1-3. doi: 10.1016/ Epub 2013 Jan 29. No abstract available.


Limitations of constant-force-feedback experiments.

Elms PJ, Chodera JD, Bustamante CJ, Marqusee S.

Biophys J. 2012 Oct 3;103(7):1490-9. doi: 10.1016/j.bpj.2012.06.051. Epub 2012 Oct 2.


Direct observation of a force-induced switch in the anisotropic mechanical unfolding pathway of a protein.

Jagannathan B, Elms PJ, Bustamante C, Marqusee S.

Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17820-5. doi: 10.1073/pnas.1201800109. Epub 2012 Sep 4.


The molten globule state is unusually deformable under mechanical force.

Elms PJ, Chodera JD, Bustamante C, Marqusee S.

Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3796-801. doi: 10.1073/pnas.1115519109. Epub 2012 Feb 21.


Propensity for C-terminal domain swapping correlates with increased regional flexibility in the C-terminus of RNase A.

Miller KH, Marqusee S.

Protein Sci. 2011 Oct;20(10):1735-44. doi: 10.1002/pro.708. Epub 2011 Sep 12.


DNA molecular handles for single-molecule protein-folding studies by optical tweezers.

Cecconi C, Shank EA, Marqusee S, Bustamante C.

Methods Mol Biol. 2011;749:255-71. doi: 10.1007/978-1-61779-142-0_18.


Structural and kinetic mapping of side-chain exposure onto the protein energy landscape.

Bernstein R, Schmidt KL, Harbury PB, Marqusee S.

Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10532-7. doi: 10.1073/pnas.1103629108. Epub 2011 Jun 13.


Protein-protein binding affinities by pulse proteolysis: application to TEM-1/BLIP protein complexes.

Hanes MS, Ratcliff K, Marqusee S, Handel TM.

Protein Sci. 2010 Oct;19(10):1996-2000. doi: 10.1002/pro.467.


The folding cooperativity of a protein is controlled by its chain topology.

Shank EA, Cecconi C, Dill JW, Marqusee S, Bustamante C.

Nature. 2010 Jun 3;465(7298):637-40. doi: 10.1038/nature09021. Epub 2010 May 23.


A hinge region cis-proline in ribonuclease A acts as a conformational gatekeeper for C-terminal domain swapping.

Miller KH, Karr JR, Marqusee S.

J Mol Biol. 2010 Jul 16;400(3):567-78. doi: 10.1016/j.jmb.2010.05.017. Epub 2010 May 13. Erratum in: J Mol Biol. 2010 Nov 12;403(5):825.


A single mutation at residue 25 populates the folding intermediate of E. coli RNase H and reveals a highly dynamic partially folded ensemble.

Connell KB, Horner GA, Marqusee S.

J Mol Biol. 2009 Aug 14;391(2):461-70. doi: 10.1016/j.jmb.2009.05.084. Epub 2009 Jun 6.


Quantitative determination of protein stability and ligand binding by pulse proteolysis.

Park C, Marqusee S.

Curr Protoc Protein Sci. 2006 Dec;Chapter 20:Unit 20.11. doi: 10.1002/0471140864.ps2011s46.


Protein-DNA chimeras for single molecule mechanical folding studies with the optical tweezers.

Cecconi C, Shank EA, Dahlquist FW, Marqusee S, Bustamante C.

Eur Biophys J. 2008 Jul;37(6):729-38. doi: 10.1007/s00249-007-0247-y. Epub 2008 Jan 9.


Exploring subdomain cooperativity in T4 lysozyme I: structural and energetic studies of a circular permutant and protein fragment.

Cellitti J, Llinas M, Echols N, Shank EA, Gillespie B, Kwon E, Crowder SM, Dahlquist FW, Alber T, Marqusee S.

Protein Sci. 2007 May;16(5):842-51. Epub 2007 Mar 30.


Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis.

Park C, Zhou S, Gilmore J, Marqusee S.

J Mol Biol. 2007 May 18;368(5):1426-37. Epub 2007 Mar 7.


A Ras-induced conformational switch in the Ras activator Son of sevenless.

Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J.

Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16692-7. Epub 2006 Oct 30.


Native state energetics of the Src SH2 domain: evidence for a partially structured state in the denatured ensemble.

Wildes D, Anderson LM, Sabogal A, Marqusee S.

Protein Sci. 2006 Jul;15(7):1769-79. Epub 2006 Jun 2.

Supplemental Content

Loading ...
Support Center