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G3 (Bethesda). 2015 Apr 3;5(6):1107-16. doi: 10.1534/g3.115.018010.

Novel Genes Critical for Hypoxic Preconditioning in Zebrafish Are Regulators of Insulin and Glucose Metabolism.

Author information

1
Division of Neonatology, University of California San Diego School of Medicine, La Jolla, California 92093.
2
Division of Respiratory Medicine, University of California San Diego School of Medicine, La Jolla, California 92093 Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, California 92093 Rady Children's Hospital-San Diego, San Diego, California 92123.
3
Division of Neonatology, University of California San Diego School of Medicine, La Jolla, California 92093 Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, California 92093 Rady Children's Hospital-San Diego, San Diego, California 92123 f2imam@ucsd.edu.

Abstract

Severe hypoxia is a common cause of major brain, heart, and kidney injury in adults, children, and newborns. However, mild hypoxia can be protective against later, more severe hypoxia exposure via "hypoxic preconditioning," a phenomenon that is not yet fully understood. Accordingly, we have established and optimized an embryonic zebrafish model to study hypoxic preconditioning. Using a functional genomic approach, we used this zebrafish model to identify and validate five novel hypoxia-protective genes, including irs2, crtc3, and camk2g2, which have been previously implicated in metabolic regulation. These results extend our understanding of the mechanisms of hypoxic preconditioning and affirm the discovery potential of this novel vertebrate hypoxic stress model.

KEYWORDS:

hormesis; hypoxia-ischemia; metabolic state; preconditioning; stress tolerance

PMID:
25840431
PMCID:
PMC4478541
DOI:
10.1534/g3.115.018010
[Indexed for MEDLINE]
Free PMC Article

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