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Arg-513 and Leu-531 Are Key Residues Governing Time-Dependent Inhibition of Cyclooxygenase-2 by Aspirin and Celebrex.

Dong L, Anderson AJ, Malkowski MG.

Biochemistry. 2019 Sep 24;58(38):3990-4002. doi: 10.1021/acs.biochem.9b00659. Epub 2019 Sep 9.


Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi.

Zhang K, Qin Z, Chang Y, Liu J, Malkowski MG, Shipa S, Li L, Qiu W, Zhang JR, Li C.

Mol Microbiol. 2019 Jun;111(6):1652-1670. doi: 10.1111/mmi.14243. Epub 2019 Apr 26.


Interactions of fatty acids, nonsteroidal anti-inflammatory drugs, and coxibs with the catalytic and allosteric subunits of cyclooxygenases-1 and -2.

Smith WL, Malkowski MG.

J Biol Chem. 2019 Feb 1;294(5):1697-1705. doi: 10.1074/jbc.TM118.006295. Review.


Residual cyclooxygenase activity of aspirin-acetylated COX-2 forms 15 R-prostaglandins that inhibit platelet aggregation.

Giménez-Bastida JA, Boeglin WE, Boutaud O, Malkowski MG, Schneider C.

FASEB J. 2019 Jan;33(1):1033-1041. doi: 10.1096/fj.201801018R. Epub 2018 Aug 10.


Mapping Protective Regions on a Three-Dimensional Model of the Moraxella catarrhalis Vaccine Antigen Oligopeptide Permease A.

Perez AC, Johnson A, Chen Z, Wilding GE, Malkowski MG, Murphy TF.

Infect Immun. 2018 Feb 20;86(3). pii: e00652-17. doi: 10.1128/IAI.00652-17. Print 2018 Mar.


A Cation-Binding Surface Protein as a Vaccine Antigen To Prevent Moraxella catarrhalis Otitis Media and Infections in Chronic Obstructive Pulmonary Disease.

Murphy TF, Brauer AL, Johnson A, Wilding GE, Koszelak-Rosenblum M, Malkowski MG.

Clin Vaccine Immunol. 2017 Sep 5;24(9). pii: e00130-17. doi: 10.1128/CVI.00130-17. Print 2017 Sep.


Fatty Acid Binding to the Allosteric Subunit of Cyclooxygenase-2 Relieves a Tonic Inhibition of the Catalytic Subunit.

Dong L, Yuan C, Orlando BJ, Malkowski MG, Smith WL.

J Biol Chem. 2016 Dec 2;291(49):25641-25655. Epub 2016 Oct 18.


Crystal structure of rofecoxib bound to human cyclooxygenase-2.

Orlando BJ, Malkowski MG.

Acta Crystallogr F Struct Biol Commun. 2016 Oct 1;72(Pt 10):772-776. Epub 2016 Sep 22.


Sulfate-binding protein, CysP, is a candidate vaccine antigen of Moraxella catarrhalis.

Murphy TF, Kirkham C, Johnson A, Brauer AL, Koszelak-Rosenblum M, Malkowski MG.

Vaccine. 2016 Jul 19;34(33):3855-61. doi: 10.1016/j.vaccine.2016.05.045. Epub 2016 Jun 14.


Substrate-selective Inhibition of Cyclooxygeanse-2 by Fenamic Acid Derivatives Is Dependent on Peroxide Tone.

Orlando BJ, Malkowski MG.

J Biol Chem. 2016 Jul 15;291(29):15069-81. doi: 10.1074/jbc.M116.725713. Epub 2016 May 20.


Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry.

Lucido MJ, Orlando BJ, Vecchio AJ, Malkowski MG.

Biochemistry. 2016 Mar 1;55(8):1226-38. doi: 10.1021/acs.biochem.5b01378. Epub 2016 Feb 19.


Pulsed Dipolar Spectroscopy Reveals That Tyrosyl Radicals Are Generated in Both Monomers of the Cyclooxygenase-2 Dimer.

Orlando BJ, Borbat PP, Georgieva ER, Freed JH, Malkowski MG.

Biochemistry. 2015 Dec 22;54(50):7309-12. doi: 10.1021/acs.biochem.5b00979. Epub 2015 Dec 7.


The Vaccine Candidate Substrate Binding Protein SBP2 Plays a Key Role in Arginine Uptake, Which Is Required for Growth of Moraxella catarrhalis.

Otsuka T, Kirkham C, Brauer A, Koszelak-Rosenblum M, Malkowski MG, Murphy TF.

Infect Immun. 2015 Nov 23;84(2):432-8. doi: 10.1128/IAI.00799-15. Print 2016 Feb.


The Crystal Structure of an Integral Membrane Fatty Acid α-Hydroxylase.

Zhu G, Koszelak-Rosenblum M, Connelly SM, Dumont ME, Malkowski MG.

J Biol Chem. 2015 Dec 11;290(50):29820-33. doi: 10.1074/jbc.M115.680124. Epub 2015 Oct 28.


Structural characterization and modeling of the Borrelia burgdorferi hybrid histidine kinase Hk1 periplasmic sensor: A system for sensing small molecules associated with tick feeding.

Bauer WJ, Luthra A, Zhu G, Radolf JD, Malkowski MG, Caimano MJ.

J Struct Biol. 2015 Oct;192(1):48-58. doi: 10.1016/j.jsb.2015.08.013. Epub 2015 Aug 28.



Anand A, LeDoyt M, Karanian C, Luthra A, Koszelak-Rosenblum M, Malkowski MG, Puthenveetil R, Vinogradova O, Radolf JD.

J Biol Chem. 2015 May 8;290(19):12313-31. doi: 10.1074/jbc.M114.629188. Epub 2015 Mar 24.


The structure of ibuprofen bound to cyclooxygenase-2.

Orlando BJ, Lucido MJ, Malkowski MG.

J Struct Biol. 2015 Jan;189(1):62-6. doi: 10.1016/j.jsb.2014.11.005. Epub 2014 Nov 25.


Role of the oligopeptide permease ABC Transporter of Moraxella catarrhalis in nutrient acquisition and persistence in the respiratory tract.

Jones MM, Johnson A, Koszelak-Rosenblum M, Kirkham C, Brauer AL, Malkowski MG, Murphy TF.

Infect Immun. 2014 Nov;82(11):4758-66. doi: 10.1128/IAI.02185-14. Epub 2014 Aug 25.


Cyclooxygenase-2 catalysis and inhibition in lipid bilayer nanodiscs.

Orlando BJ, McDougle DR, Lucido MJ, Eng ET, Graham LA, Schneider C, Stokes DL, Das A, Malkowski MG.

Arch Biochem Biophys. 2014 Mar 15;546:33-40. doi: 10.1016/ Epub 2014 Feb 3.


Crystal structures of α-dioxygenase from Oryza sativa: insights into substrate binding and activation by hydrogen peroxide.

Zhu G, Koszelak-Rosenblum M, Malkowski MG.

Protein Sci. 2013 Oct;22(10):1432-8. doi: 10.1002/pro.2327. Epub 2013 Sep 4.


Role of the zinc uptake ABC transporter of Moraxella catarrhalis in persistence in the respiratory tract.

Murphy TF, Brauer AL, Kirkham C, Johnson A, Koszelak-Rosenblum M, Malkowski MG.

Infect Immun. 2013 Sep;81(9):3406-13. doi: 10.1128/IAI.00589-13. Epub 2013 Jul 1.


Structure of the integral membrane protein CAAX protease Ste24p.

Pryor EE Jr, Horanyi PS, Clark KM, Fedoriw N, Connelly SM, Koszelak-Rosenblum M, Zhu G, Malkowski MG, Wiener MC, Dumont ME.

Science. 2013 Mar 29;339(6127):1600-4. doi: 10.1126/science.1232048.


Coordinating the impact of structural genomics on the human α-helical transmembrane proteome.

Pieper U, Schlessinger A, Kloppmann E, Chang GA, Chou JJ, Dumont ME, Fox BG, Fromme P, Hendrickson WA, Malkowski MG, Rees DC, Stokes DL, Stowell MH, Wiener MC, Rost B, Stroud RM, Stevens RC, Sali A.

Nat Struct Mol Biol. 2013 Feb;20(2):135-8. doi: 10.1038/nsmb.2508. No abstract available.


The crystal structure of α-Dioxygenase provides insight into diversity in the cyclooxygenase-peroxidase superfamily.

Goulah CC, Zhu G, Koszelak-Rosenblum M, Malkowski MG.

Biochemistry. 2013 Feb 26;52(8):1364-72. doi: 10.1021/bi400013k. Epub 2013 Feb 14.


Investigating substrate promiscuity in cyclooxygenase-2: the role of Arg-120 and residues lining the hydrophobic groove.

Vecchio AJ, Orlando BJ, Nandagiri R, Malkowski MG.

J Biol Chem. 2012 Jul 13;287(29):24619-30. doi: 10.1074/jbc.M112.372243. Epub 2012 May 25.


The transition from closed to open conformation of Treponema pallidum outer membrane-associated lipoprotein TP0453 involves membrane sensing and integration by two amphipathic helices.

Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, Malkowski MG, Radolf JD.

J Biol Chem. 2011 Dec 2;286(48):41656-68. doi: 10.1074/jbc.M111.305284. Epub 2011 Sep 29.


The structure of NS-398 bound to cyclooxygenase-2.

Vecchio AJ, Malkowski MG.

J Struct Biol. 2011 Nov;176(2):254-8. doi: 10.1016/j.jsb.2011.07.019. Epub 2011 Aug 6.


The structural basis of endocannabinoid oxygenation by cyclooxygenase-2.

Vecchio AJ, Malkowski MG.

J Biol Chem. 2011 Jun 10;286(23):20736-45. doi: 10.1074/jbc.M111.230367. Epub 2011 Apr 13.


Human cyclooxygenase-2 is a sequence homodimer that functions as a conformational heterodimer.

Dong L, Vecchio AJ, Sharma NP, Jurban BJ, Malkowski MG, Smith WL.

J Biol Chem. 2011 May 27;286(21):19035-46. doi: 10.1074/jbc.M111.231969. Epub 2011 Apr 5.


Structural basis of fatty acid substrate binding to cyclooxygenase-2.

Vecchio AJ, Simmons DM, Malkowski MG.

J Biol Chem. 2010 Jul 16;285(29):22152-63. doi: 10.1074/jbc.M110.119867. Epub 2010 May 12.


Pleiotropic mechanisms facilitated by resveratrol and its metabolites.

Calamini B, Ratia K, Malkowski MG, Cuendet M, Pezzuto JM, Santarsiero BD, Mesecar AD.

Biochem J. 2010 Jul 15;429(2):273-82. doi: 10.1042/BJ20091857.


Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.

Clark KM, Fedoriw N, Robinson K, Connelly SM, Randles J, Malkowski MG, DeTitta GT, Dumont ME.

Protein Expr Purif. 2010 Jun;71(2):207-23. doi: 10.1016/j.pep.2009.12.012. Epub 2010 Jan 4.


Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins.

Koszelak-Rosenblum M, Krol A, Mozumdar N, Wunsch K, Ferin A, Cook E, Veatch CK, Nagel R, Luft JR, Detitta GT, Malkowski MG.

Protein Sci. 2009 Sep;18(9):1828-39. doi: 10.1002/pro.193.


Establishing a training set through the visual analysis of crystallization trials. Part II: crystal examples.

Snell EH, Lauricella AM, Potter SA, Luft JR, Gulde SM, Collins RJ, Franks G, Malkowski MG, Cumbaa C, Jurisica I, DeTitta GT.

Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1131-7. doi: 10.1107/S0907444908028059. Epub 2008 Oct 18.


Establishing a training set through the visual analysis of crystallization trials. Part I: approximately 150,000 images.

Snell EH, Luft JR, Potter SA, Lauricella AM, Gulde SM, Malkowski MG, Koszelak-Rosenblum M, Said MI, Smith JL, Veatch CK, Collins RJ, Franks G, Thayer M, Cumbaa C, Jurisica I, Detitta GT.

Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1123-30. doi: 10.1107/S0907444908028047. Epub 2008 Oct 18.


His-311 and Arg-559 are key residues involved in fatty acid oxygenation in pathogen-inducible oxygenase.

Koszelak-Rosenblum M, Krol AC, Simmons DM, Goulah CC, Wroblewski L, Malkowski MG.

J Biol Chem. 2008 Sep 5;283(36):24962-71. doi: 10.1074/jbc.M804358200. Epub 2008 Jul 2.


Structural genomics: from genes to structures with valuable materials and many questions in between.

Fox BG, Goulding C, Malkowski MG, Stewart L, Deacon A.

Nat Methods. 2008 Feb;5(2):129-32. doi: 10.1038/nmeth0208-129. Review.


Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.

Malkowski MG, Quartley E, Friedman AE, Babulski J, Kon Y, Wolfley J, Said M, Luft JR, Phizicky EM, DeTitta GT, Grayhack EJ.

Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6678-83. Epub 2007 Apr 10.


Efficient optimization of crystallization conditions by manipulation of drop volume ratio and temperature.

Luft JR, Wolfley JR, Said MI, Nagel RM, Lauricella AM, Smith JL, Thayer MH, Veatch CK, Snell EH, Malkowski MG, Detitta GT.

Protein Sci. 2007 Apr;16(4):715-22. Epub 2007 Feb 27.


The structures of prostaglandin endoperoxide H synthases-1 and -2.

Garavito RM, Malkowski MG, DeWitt DL.

Prostaglandins Other Lipid Mediat. 2002 Aug;68-69:129-52. Review.


Structure of eicosapentaenoic and linoleic acids in the cyclooxygenase site of prostaglandin endoperoxide H synthase-1.

Malkowski MG, Thuresson ED, Lakkides KM, Rieke CJ, Micielli R, Smith WL, Garavito RM.

J Biol Chem. 2001 Oct 5;276(40):37547-55. Epub 2001 Jul 27.


Mutational and X-ray crystallographic analysis of the interaction of dihomo-gamma -linolenic acid with prostaglandin endoperoxide H synthases.

Thuresson ED, Malkowski MG, Lakkides KM, Rieke CJ, Mulichak AM, Ginell SL, Garavito RM, Smith WL.

J Biol Chem. 2001 Mar 30;276(13):10358-65. Epub 2000 Dec 19.


Prostaglandin endoperoxide H synthase-1: the functions of cyclooxygenase active site residues in the binding, positioning, and oxygenation of arachidonic acid.

Thuresson ED, Lakkides KM, Rieke CJ, Sun Y, Wingerd BA, Micielli R, Mulichak AM, Malkowski MG, Garavito RM, Smith WL.

J Biol Chem. 2001 Mar 30;276(13):10347-57. Epub 2000 Dec 19.


The productive conformation of arachidonic acid bound to prostaglandin synthase.

Malkowski MG, Ginell SL, Smith WL, Garavito RM.

Science. 2000 Sep 15;289(5486):1933-7.


The formation of stable fatty acid substrate complexes in prostaglandin H(2) synthase-1.

Malkowski MG, Theisen MJ, Scharmen A, Garavito RM.

Arch Biochem Biophys. 2000 Aug 1;380(1):39-45.


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