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G3 (Bethesda). 2015 Oct 13;5(12):2729-43. doi: 10.1534/g3.115.020974.

Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa.

Author information

1
Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, California 92521.
2
Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
3
Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521.
4
College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
5
Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, California 92521 Katherine.Borkovich@ucr.edu.

Abstract

G protein-coupled receptors (GPCRs) regulate facets of growth, development, and environmental sensing in eukaryotes, including filamentous fungi. The largest predicted GPCR class in these organisms is the Pth11-related, with members similar to a protein required for disease in the plant pathogen Magnaporthe oryzae. However, the Pth11-related class has not been functionally studied in any filamentous fungal species. Here, we analyze phenotypes in available mutants for 36 GPCR genes, including 20 Pth11-related, in the model filamentous fungus Neurospora crassa. We also investigate patterns of gene expression for all 43 predicted GPCR genes in available datasets. A total of 17 mutants (47%) possessed at least one growth or developmental phenotype. We identified 18 mutants (56%) with chemical sensitivity or nutritional phenotypes (11 uniquely), bringing the total number of mutants with at least one defect to 28 (78%), including 15 mutants (75%) in the Pth11-related class. Gene expression trends for GPCR genes correlated with the phenotypes observed for many mutants and also suggested overlapping functions for several groups of co-transcribed genes. Several members of the Pth11-related class have phenotypes and/or are differentially expressed on cellulose, suggesting a possible role for this gene family in plant cell wall sensing or utilization.

KEYWORDS:

G protein−coupled receptors; Heterotrimeric G proteins; filamentous fungi; functional genomics; signal transduction

PMID:
26464358
PMCID:
PMC4683645
DOI:
10.1534/g3.115.020974
[Indexed for MEDLINE]
Free PMC Article

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