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Wellcome Open Res. 2018 Jan 9;3:3. doi: 10.12688/wellcomeopenres.13488.1. eCollection 2018.

Transplantation of schistosome sporocysts between host snails: A video guide.

Author information

1
Laboratoire Interactions Hôtes-Pathogènes-Environnements (IHPE), UMR 5244 CNRS/UPVD/IFREMER/UM, University of Perpignan Via Domitia, 58 Avenue Paul Alduy, Bât R, F-66860 Perpignan Cedex, France.
2
Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK.
3
Institute of Biological, Environmental and Rural Sciences , Aberystwyth University, Aberystwyth, UK.
4
Department of Microbiology, Immunology & Tropical Medicine, George Washington University Medical Center, Washington, DC, USA.
5
Institut für Parasitologie, Justus-Liebig-Universität Gießen, Gießen, Germany.
6
Department of Zoology, University of Cambridge, Downing St, Cambridge, UK.
7
Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX, USA.

Abstract

Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.

KEYWORDS:

Biomphalaria; Schistosomiasis; sporocyst transfer; video instruction

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