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Dev Biol. 2018 Mar 15;435(2):176-184. doi: 10.1016/j.ydbio.2018.01.021. Epub 2018 Feb 2.

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth.

Author information

1
Department of Physiology, Pharmacology and Neuroscience, University of Bristol, BS8 1TD, United Kingdom; Centro de Pesquisa sobre o Genoma Humano e Células Tronco, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Brazil. Electronic address: erika.kague@bristol.ac.uk.
2
Evolutionary Developmental Biology, Ghent University, Belgium.
3
Centro de Pesquisa sobre o Genoma Humano e Células Tronco, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Brazil.
4
Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, United States.
5
Department of Physiology, Pharmacology and Neuroscience, University of Bristol, BS8 1TD, United Kingdom.
6
School of Archaeology and Anthropology, University of Bristol, United Kingdom.

Abstract

The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.

PMID:
29409769
DOI:
10.1016/j.ydbio.2018.01.021
[Indexed for MEDLINE]
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