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The Need for Accuracy in Hemoglobin A1c Proficiency Testing: Why the Proposed CLIA Rule of 2019 Is a Step Backward.

Klonoff DC, Aron D, Cohen RM, Home P, John WG, Little RR, Nathan DM, Sacks DB.

J Diabetes Sci Technol. 2019 May;13(3):424-427. doi: 10.1177/1932296819841699. Epub 2019 Mar 22. No abstract available.


Accuracy and Precision of a Point-of-Care HbA1c Test.

Arnold WD, Kupfer K, Little RR, Amar M, Horowitz B, Godbole N, Hvidsten Swensen M, Li Y, San George RC.

J Diabetes Sci Technol. 2019 Mar 10:1932296819831292. doi: 10.1177/1932296819831292. [Epub ahead of print]


The National Glycohemoglobin Standardization Program: Over 20 Years of Improving Hemoglobin A1c Measurement.

Little RR, Rohlfing C, Sacks DB.

Clin Chem. 2019 Jul;65(7):839-848. doi: 10.1373/clinchem.2018.296962. Epub 2018 Dec 5. Review.


Multicenter assessment of a hemoglobin A1c point-of-care device for diagnosis of diabetes mellitus.

Sobolesky PM, Smith BE, Saenger AK, Schulz K, Apple FS, Scott MG, Wu AHB, Little RR, Fitzgerald RL.

Clin Biochem. 2018 Nov;61:18-22. doi: 10.1016/j.clinbiochem.2018.09.007. Epub 2018 Sep 17.


Recognition of rare hemoglobin variants by hemoglobin A1c measurement procedures.

Strickland SW, Campbell ST, Little RR, Bruns DE, Bazydlo LAL.

Clin Chim Acta. 2018 Jan;476:67-74. doi: 10.1016/j.cca.2017.11.012. Epub 2017 Nov 14.


Implementing a Reference Measurement System for C-Peptide: An Addendum.

Little RR, Kinumi T, Connolly S, Kabytaev K.

Clin Chem. 2017 Dec;63(12):1904-1905. doi: 10.1373/clinchem.2017.281170. Epub 2017 Oct 4. No abstract available.


Prevalence of Rare Hemoglobin Variants Identified During Measurements of Hb A1c by Capillary Electrophoresis.

Strickland SW, Campbell ST, Little RR, Bruns DE, Bazydlo LAL.

Clin Chem. 2017 Dec;63(12):1901-1902. doi: 10.1373/clinchem.2017.276857. Epub 2017 Sep 13. No abstract available.


Implementing a Reference Measurement System for C-Peptide: Successes and Lessons Learned.

Little RR, Wielgosz RI, Josephs R, Kinumi T, Takatsu A, Li H, Stein D, Burns C.

Clin Chem. 2017 Sep;63(9):1447-1456. doi: 10.1373/clinchem.2016.269274. Epub 2017 Jun 23. Review.


Measurement of Hemoglobin A1c in Patients With Sickle Cell Trait.

Rohlfing C, Hanson S, Little RR.

JAMA. 2017 Jun 6;317(21):2237. doi: 10.1001/jama.2017.4643. No abstract available.


Validation of the Use of Trinity Biotech ultra2 as a Comparative Method for Hemoglobin A1c Measurements in the Presence of HbE and HbD-Punjab Traits.

Rohlfing C, Connolly S, Hanson S, Higgins T, Little RR.

Clin Chem. 2017 Feb;63(2):608-610. doi: 10.1373/clinchem.2016.266221. Epub 2016 Dec 15. No abstract available.


Higher degree of glycation of hemoglobin S compared to hemoglobin A measured by mass spectrometry: Potential impact on HbA1c testing.

Kabytaev K, Connolly S, Rohlfing CL, Sacks DB, Stoyanov AV, Little RR.

Clin Chim Acta. 2016 Jul 1;458:40-3. doi: 10.1016/j.cca.2016.04.027. Epub 2016 Apr 22.


Effects of hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by twelve methods.

Rohlfing C, Hanson S, Weykamp C, Siebelder C, Higgins T, Molinaro R, Yip PM, Little RR.

Clin Chim Acta. 2016 Apr 1;455:80-3. doi: 10.1016/j.cca.2016.01.031. Epub 2016 Jan 29.


Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis.

Kabytaev K, Durairaj A, Shin D, Rohlfing CL, Connolly S, Little RR, Stoyanov AV.

J Sep Sci. 2016 Feb;39(4):676-81. doi: 10.1002/jssc.201500989. Epub 2016 Jan 28.


Assessing quality from an accuracy-based HbA1c proficiency survey.

Little RR, Rohlfing CL.

Clin Chem Lab Med. 2016 Mar;54(3):e75-6. doi: 10.1515/cclm-2015-0972. No abstract available.


Quality of HbA1c Measurement in Trinidad and Tobago.

Rastogi MV, Ladenson P, Goldstein DE, Little RR.

J Diabetes Sci Technol. 2016 May 3;10(3):768-71. doi: 10.1177/1932296815609620. Print 2016 May.


Evaluation of hemoglobin A1c measurement by Capillarys 2 electrophoresis for detection of abnormal glucose tolerance in African immigrants to the United States.

Zhao Z, Basilio J, Hanson S, Little RR, Sumner AE, Sacks DB.

Clin Chim Acta. 2015 Jun 15;446:54-60. doi: 10.1016/j.cca.2015.03.025. Epub 2015 Apr 8.


Investigation of 2 models to set and evaluate quality targets for hb a1c: biological variation and sigma-metrics.

Weykamp C, John G, Gillery P, English E, Ji L, Lenters-Westra E, Little RR, Roglic G, Sacks DB, Takei I; IFCC Task Force on Implementation of HbA1c Standardization.

Clin Chem. 2015 May;61(5):752-9. doi: 10.1373/clinchem.2014.235333. Epub 2015 Mar 3.


Effects of 49 Different Rare Hb Variants on HbA1c Measurement in Eight Methods.

Little RR, La'ulu SL, Hanson SE, Rohlfing CL, Schmidt RL.

J Diabetes Sci Technol. 2015 Jul;9(4):849-56. doi: 10.1177/1932296815572367. Epub 2015 Feb 17.


Performance of hemoglobin A1c assay methods: good enough?

Little RR.

Clin Chem. 2014 Aug;60(8):1031-3. doi: 10.1373/clinchem.2014.225789. Epub 2014 Jun 17. No abstract available.


Comparing analytic performance criteria: evaluation of HbA1c certification criteria as an example.

Rohlfing CL, Parvin CA, Sacks DB, Little RR; NGSP Steering Committee.

Clin Chim Acta. 2014 Jun 10;433:259-63. doi: 10.1016/j.cca.2014.03.034. Epub 2014 Apr 8.


Isotope dilution assay in peptide quantification: the challenge of microheterogeneity of internal standard.

Stoyanov AV, Rogatsky E, Stein D, Connolly S, Rohlfing CL, Little RR.

Proteomics Clin Appl. 2013 Dec;7(11-12):825-8. doi: 10.1002/prca.201200130. Epub 2013 Oct 18.


Measurement of Hba(1C) in patients with chronic renal failure.

Little RR, Rohlfing CL, Tennill AL, Hanson SE, Connolly S, Higgins T, Wiedmeyer CE, Weykamp CW, Krause R, Roberts W.

Clin Chim Acta. 2013 Mar 15;418:73-6. doi: 10.1016/j.cca.2012.12.022. Epub 2013 Jan 12.


The long and winding road to optimal HbA1c measurement.

Little RR, Rohlfing CL.

Clin Chim Acta. 2013 Mar 15;418:63-71. doi: 10.1016/j.cca.2012.12.026. Epub 2013 Jan 11. Review.


Comparison of hemoglobin A1c measurements of samples with elevated fetal hemoglobin by three commercial assays.

Shu I, Devaraj S, Hanson SE, Little RR, Wang P.

Clin Chim Acta. 2012 Oct 9;413(19-20):1712-3. doi: 10.1016/j.cca.2012.05.017. Epub 2012 May 26. No abstract available.


The effect of increased fetal hemoglobin on 7 common Hb A1c assay methods.

Little RR, Rohlfing CL, Hanson SE, Schmidt RL, Lin CN, Madsen RW, Roberts WL.

Clin Chem. 2012 May;58(5):945-7. doi: 10.1373/clinchem.2012.181933. Epub 2012 Feb 22. No abstract available.


Effects of hemoglobin C, D, E, and S traits on measurements of HbA1c by six methods.

Lin CN, Emery TJ, Little RR, Hanson SE, Rohlfing CL, Jaisson S, Gillery P, Roberts WL.

Clin Chim Acta. 2012 Apr 11;413(7-8):819-21. doi: 10.1016/j.cca.2011.12.019. Epub 2012 Jan 8. No abstract available.


Usefulness of glycated albumin assay for diabetes monitoring.

Little RR.

J Diabetes Sci Technol. 2011 Nov 1;5(6):1463-5.


Use of cation exchange chromatography for human C-peptide isotope dilution - mass spectrometric assay.

Stoyanov AV, Rohlfing CL, Connolly S, Roberts ML, Nauser CL, Little RR.

J Chromatogr A. 2011 Dec 23;1218(51):9244-9. doi: 10.1016/j.chroma.2011.10.080. Epub 2011 Nov 2.


Effects of whole blood storage on hemoglobin a1c measurements with five current assay methods.

Rohlfing CL, Hanson S, Tennill AL, Little RR.

Diabetes Technol Ther. 2012 Mar;14(3):271-5. doi: 10.1089/dia.2011.0136. Epub 2011 Oct 27.


HbA(1c): what do the numbers really mean?

Little RR, Sacks DB.

Lancet. 2011 Sep 17;378(9796):1068-9; author reply 1069-70. doi: 10.1016/S0140-6736(11)61473-6. No abstract available.


Point-of-care assays for hemoglobin A(1c): is performance adequate?

Little RR, Lenters-Westra E, Rohlfing CL, Slingerland R.

Clin Chem. 2011 Sep;57(9):1333-4. doi: 10.1373/clinchem.2011.165019. Epub 2011 Jun 28. No abstract available.


Analytical goals for HbA1c: are HbA1c results good enough for optimal use?

Little RR, Rohlfing CL.

J Diabetes. 2011 Mar;3(1):3-6. doi: 10.1111/j.1753-0407.2010.00109.x. No abstract available.



Little RR.

Clin Chem. 2011 Feb;57(2):156. doi: 10.1373/clinchem.2010.157610. No abstract available.


Status of hemoglobin A1c measurement and goals for improvement: from chaos to order for improving diabetes care.

Little RR, Rohlfing CL, Sacks DB; National Glycohemoglobin Standardization Program (NGSP) Steering Committee.

Clin Chem. 2011 Feb;57(2):205-14. doi: 10.1373/clinchem.2010.148841. Epub 2010 Dec 9. Review.


Effects of hemoglobin C and S traits on Tosoh G8 and Siemens Advia HbA1c assays.

Little RR, Rohlfing CL, Hanson SE, Roberts WL.

Clin Chim Acta. 2010 May 2;411(9-10):779-80. doi: 10.1016/j.cca.2010.02.012. Epub 2010 Feb 19. No abstract available.


A review of variant hemoglobins interfering with hemoglobin A1c measurement.

Little RR, Roberts WL.

J Diabetes Sci Technol. 2009 May 1;3(3):446-51. Review.


HbA1c: how do we measure it and what does it mean?

Little RR, Sacks DB.

Curr Opin Endocrinol Diabetes Obes. 2009 Apr;16(2):113-8. doi: 10.1097/MED.0b013e328327728d. Review.


Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods.

Little RR, Rohlfing CL, Hanson S, Connolly S, Higgins T, Weykamp CW, D'Costa M, Luzzi V, Owen WE, Roberts WL.

Clin Chem. 2008 Aug;54(8):1277-82. doi: 10.1373/clinchem.2008.103580. Epub 2008 Jun 12.


Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assays.

Mongia SK, Little RR, Rohlfing CL, Hanson S, Roberts RF, Owen WE, D'Costa MA, Reyes CA, Luzzi VI, Roberts WL.

Am J Clin Pathol. 2008 Jul;130(1):136-40. doi: 10.1309/1YU0D34VJKNUCGT1.


The effect of elevated fetal hemoglobin on hemoglobin A1c results: five common hemoglobin A1c methods compared with the IFCC reference method.

Rohlfing CL, Connolly SM, England JD, Hanson SE, Moellering CM, Bachelder JR, Little RR.

Am J Clin Pathol. 2008 May;129(5):811-4. doi: 10.1309/YFVTUD0GHJF7D16H.


Standardization of C-peptide measurements.

Little RR, Rohlfing CL, Tennill AL, Madsen RW, Polonsky KS, Myers GL, Greenbaum CJ, Palmer JP, Rogatsky E, Stein DT.

Clin Chem. 2008 Jun;54(6):1023-6. doi: 10.1373/clinchem.2007.101287. Epub 2008 Apr 17.


Trends in A1C concentrations among U.S. adults with diagnosed diabetes from 1999 to 2004.

Ford ES, Li C, Little RR, Mokdad AH.

Diabetes Care. 2008 Jan;31(1):102-4. Epub 2007 Oct 12. No abstract available.


Global standardization of glycated hemoglobin measurement: the position of the IFCC Working Group.

International Federation of Clinical Chemistry and Laboratory Medicine, IFCC Scientific Division, Mosca A, Goodall I, Hoshino T, Jeppsson JO, John WG, Little RR, Miedema K, Myers GL, Reinauer H, Sacks DB, Weykamp CW.

Clin Chem Lab Med. 2007;45(8):1077-80.


International comparison of C-peptide measurements.

Wiedmeyer HM, Polonsky KS, Myers GL, Little RR, Greenbaum CJ, Goldstein DE, Palmer JP.

Clin Chem. 2007 Apr;53(4):784-7. Epub 2007 Mar 1.


Effects of sample storage conditions on glycated hemoglobin measurement: evaluation of five different high performance liquid chromatography methods.

Little RR, Rohlfing CL, Tennill AL, Connolly S, Hanson S.

Diabetes Technol Ther. 2007 Feb;9(1):36-42.


Trends in hyperinsulinemia among nondiabetic adults in the U.S.

Li C, Ford ES, McGuire LC, Mokdad AH, Little RR, Reaven GM.

Diabetes Care. 2006 Nov;29(11):2396-402.


Analysis: point-of-care testing for glycated hemoglobin (GHB).

Little RR.

Diabetes Technol Ther. 2005 Dec;7(6):913-5. No abstract available.


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