Format

Send to

Choose Destination
Antimicrob Agents Chemother. 2015 Dec;59(12):7387-95. doi: 10.1128/AAC.01458-15. Epub 2015 Sep 21.

Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India.

Author information

1
School of Pharmacy, University of São Paulo, São Paulo, Brazil.
2
Fleury Group, Microbiology Section, Rio de Janeiro, Brazil.
3
Children's Hospital, Rio de Janeiro, Brazil.
4
Research Laboratory of Bacterial Resistance, Experimental Research Center, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
5
Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo, São Paulo, Brazil.
6
Núcleo de Bacteriologia, GBM/Laboratório Central de Saúde Pública, Lacen-Brasilia, Brasília, Brazil.
7
Core Facility for Scientific Research, University of São Paulo, São Paulo, Brazil.
8
Microbiology Department, Federal University of São Paulo, São Paulo, Brazil.
9
Research Laboratory of Bacterial Resistance, Experimental Research Center, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil Infectious Diseases Service, Hospital de Clínicas de Porto, Porto Alegre, Brazil.
10
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
11
School of Pharmacy, University of São Paulo, São Paulo, Brazil Fleury Diagnostic Medicine, Microbiology Section, São Paulo, Brazil sampaio@usp.br.

Abstract

In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world.

PMID:
26392506
PMCID:
PMC4649174
DOI:
10.1128/AAC.01458-15
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center