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Items: 21


Improving probes for super-resolution.

Moore RP, Legant WR.

Nat Methods. 2018 Sep;15(9):659-660. doi: 10.1038/s41592-018-0120-1. No abstract available.


Applying systems-level spectral imaging and analysis to reveal the organelle interactome.

Valm AM, Cohen S, Legant WR, Melunis J, Hershberg U, Wait E, Cohen AR, Davidson MW, Betzig E, Lippincott-Schwartz J.

Nature. 2017 Jun 1;546(7656):162-167. doi: 10.1038/nature22369. Epub 2017 May 24.


Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy.

Aguet F, Upadhyayula S, Gaudin R, Chou YY, Cocucci E, He K, Chen BC, Mosaliganti K, Pasham M, Skillern W, Legant WR, Liu TL, Findlay G, Marino E, Danuser G, Megason S, Betzig E, Kirchhausen T.

Mol Biol Cell. 2016 Nov 7;27(22):3418-3435. Epub 2016 Aug 17.


Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER.

Nixon-Abell J, Obara CJ, Weigel AV, Li D, Legant WR, Xu CS, Pasolli HA, Harvey K, Hess HF, Betzig E, Blackstone C, Lippincott-Schwartz J.

Science. 2016 Oct 28;354(6311). pii: aaf3928. Epub 2016 Oct 27.


Real-time imaging of Huntingtin aggregates diverting target search and gene transcription.

Li L, Liu H, Dong P, Li D, Legant WR, Grimm JB, Lavis LD, Betzig E, Tjian R, Liu Z.

Elife. 2016 Aug 3;5. pii: e17056. doi: 10.7554/eLife.17056.


High-density three-dimensional localization microscopy across large volumes.

Legant WR, Shao L, Grimm JB, Brown TA, Milkie DE, Avants BB, Lavis LD, Betzig E.

Nat Methods. 2016 Apr;13(4):359-65. doi: 10.1038/nmeth.3797. Epub 2016 Mar 7.


Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus.

Yamashita N, Morita M, Legant WR, Chen BC, Betzig E, Yokota H, Mimori-Kiyosue Y.

J Biomed Opt. 2015 Oct;20(10):101206. doi: 10.1117/1.JBO.20.10.101206.


Asymmetric formation of coated pits on dorsal and ventral surfaces at the leading edges of motile cells and on protrusions of immobile cells.

Kural C, Akatay AA, Gaudin R, Chen BC, Legant WR, Betzig E, Kirchhausen T.

Mol Biol Cell. 2015 Jun 1;26(11):2044-53. doi: 10.1091/mbc.E15-01-0055. Epub 2015 Apr 7.


3D imaging of Sox2 enhancer clusters in embryonic stem cells.

Liu Z, Legant WR, Chen BC, Li L, Grimm JB, Lavis LD, Betzig E, Tjian R.

Elife. 2014 Dec 24;3:e04236. doi: 10.7554/eLife.04236.


Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.

Chen BC, Legant WR, Wang K, Shao L, Milkie DE, Davidson MW, Janetopoulos C, Wu XS, Hammer JA 3rd, Liu Z, English BP, Mimori-Kiyosue Y, Romero DP, Ritter AT, Lippincott-Schwartz J, Fritz-Laylin L, Mullins RD, Mitchell DM, Bembenek JN, Reymann AC, Böhme R, Grill SW, Wang JT, Seydoux G, Tulu US, Kiehart DP, Betzig E.

Science. 2014 Oct 24;346(6208):1257998. doi: 10.1126/science.1257998. Epub 2014 Oct 23.


Microfabrication of a platform to measure and manipulate the mechanics of engineered microtissues.

Ramade A, Legant WR, Picart C, Chen CS, Boudou T.

Methods Cell Biol. 2014;121:191-211. doi: 10.1016/B978-0-12-800281-0.00013-0.


Carbofluoresceins and carborhodamines as scaffolds for high-contrast fluorogenic probes.

Grimm JB, Sung AJ, Legant WR, Hulamm P, Matlosz SM, Betzig E, Lavis LD.

ACS Chem Biol. 2013;8(6):1303-10. doi: 10.1021/cb4000822. Epub 2013 Apr 24.


Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels.

Khetan S, Guvendiren M, Legant WR, Cohen DM, Chen CS, Burdick JA.

Nat Mater. 2013 May;12(5):458-65. doi: 10.1038/nmat3586. Epub 2013 Mar 24.


Physically-induced cytoskeleton remodeling of cells in three-dimensional culture.

Lee SL, Nekouzadeh A, Butler B, Pryse KM, McConnaughey WB, Nathan AC, Legant WR, Schaefer PM, Pless RB, Elson EL, Genin GM.

PLoS One. 2012;7(12):e45512. doi: 10.1371/journal.pone.0045512. Epub 2012 Dec 27.


Multidimensional traction force microscopy reveals out-of-plane rotational moments about focal adhesions.

Legant WR, Choi CK, Miller JS, Shao L, Gao L, Betzig E, Chen CS.

Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):881-6. doi: 10.1073/pnas.1207997110. Epub 2012 Dec 31.


Development and characterization of a 3D multicell microtissue culture model of airway smooth muscle.

West AR, Zaman N, Cole DJ, Walker MJ, Legant WR, Boudou T, Chen CS, Favreau JT, Gaudette GR, Cowley EA, Maksym GN.

Am J Physiol Lung Cell Mol Physiol. 2013 Jan 1;304(1):L4-16. doi: 10.1152/ajplung.00168.2012. Epub 2012 Nov 2.


A microfabricated platform to measure and manipulate the mechanics of engineered cardiac microtissues.

Boudou T, Legant WR, Mu A, Borochin MA, Thavandiran N, Radisic M, Zandstra PW, Epstein JA, Margulies KB, Chen CS.

Tissue Eng Part A. 2012 May;18(9-10):910-9. doi: 10.1089/ten.TEA.2011.0341. Epub 2012 Jan 4.


Measurement of mechanical tractions exerted by cells in three-dimensional matrices.

Legant WR, Miller JS, Blakely BL, Cohen DM, Genin GM, Chen CS.

Nat Methods. 2010 Dec;7(12):969-71. doi: 10.1038/nmeth.1531. Epub 2010 Nov 14.


Bioactive hydrogels made from step-growth derived PEG-peptide macromers.

Miller JS, Shen CJ, Legant WR, Baranski JD, Blakely BL, Chen CS.

Biomaterials. 2010 May;31(13):3736-43. doi: 10.1016/j.biomaterials.2010.01.058. Epub 2010 Feb 6.


Microfabricated tissue gauges to measure and manipulate forces from 3D microtissues.

Legant WR, Pathak A, Yang MT, Deshpande VS, McMeeking RM, Chen CS.

Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10097-102. doi: 10.1073/pnas.0900174106. Epub 2009 Jun 16.

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