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Evaluation of the WASPLab software to Automatically Read CHROMID CPS Elite Agar for Reporting of Urine Cultures.

Faron ML, Buchan BW, Samra H, Ledeboer NA.

J Clin Microbiol. 2019 Nov 6. pii: JCM.00540-19. doi: 10.1128/JCM.00540-19. [Epub ahead of print]


Multicenter Study Demonstrates Standardization Requirements for Mold Identification by MALDI-TOF MS.

Lau AF, Walchak RC, Miller HB, Slechta ES, Kamboj K, Riebe K, Robertson AE, Gilbreath JJ, Mitchell KF, Wallace MA, Bryson AL, Balada-Llasat JM, Bulman A, Buchan BW, Burnham CD, Butler-Wu S, Desai U, Doern CD, Hanson KE, Henderson CM, Kostrzewa M, Ledeboer NA, Maier T, Pancholi P, Schuetz AN, Shi G, Wengenack NL, Zhang SX, Zelazny AM, Frank KM.

Front Microbiol. 2019 Sep 20;10:2098. doi: 10.3389/fmicb.2019.02098. eCollection 2019.


Evaluation of a Novel Multiplex High-Definition PCR Assay for Detection of Tick-Borne Pathogens in Whole-Blood Specimens.

Buchan BW, Jobe DA, Mashock M, Gerstbrein D, Faron ML, Ledeboer NA, Callister SM.

J Clin Microbiol. 2019 Oct 23;57(11). pii: e00513-19. doi: 10.1128/JCM.00513-19. Print 2019 Nov.


Use of a cohorting-unit and systematic surveillance cultures to control a Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae outbreak.

Reeme AE, Bowler SL, Buchan BW, Graham MB, Behrens E, Singh S, Hong JC, Arvan J, Hyke JW, Palen L, Savage S, Seliger H, Huerta S, Ledeboer NA, Kotay S, Mathers AJ, Cooper VS, Mustapha MM, Mettus RT, Doi Y, Munoz-Price LS.

Infect Control Hosp Epidemiol. 2019 Jul;40(7):767-773. doi: 10.1017/ice.2019.99. Epub 2019 May 14.


Diagnosing and Managing Sepsis by Probing the Host Response to Infection: Advances, Opportunities, and Challenges.

Gunsolus IL, Sweeney TE, Liesenfeld O, Ledeboer NA.

J Clin Microbiol. 2019 Jun 25;57(7). pii: e00425-19. doi: 10.1128/JCM.00425-19. Print 2019 Jul. Review.


The relevance of sink proximity to toilets on the detection of Klebsiella pneumoniae carbapenemase inside sink drains.

Buchan BW, Graham MB, Lindmair-Snell J, Arvan J, Ledeboer NA, Nanchal R, Munoz-Price LS.

Am J Infect Control. 2019 Jan;47(1):98-100. doi: 10.1016/j.ajic.2018.06.021. Epub 2018 Aug 29.


Multicenter evaluation of the Alere™ i influenza A&B assay using respiratory specimens collected in viral transport media.

Hassan F, Crawford J, Bonner AB, Ledeboer NA, Selvarangan R.

Diagn Microbiol Infect Dis. 2018 Dec;92(4):294-298. doi: 10.1016/j.diagmicrobio.2018.07.002. Epub 2018 Jul 7.


Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures.

Granato PA, Unz MM, Widen RH, Silbert S, Young S, Heflin KL, Conover MS, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2018 Aug 27;56(9). pii: e00485-18. doi: 10.1128/JCM.00485-18. Print 2018 Sep.


Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis.

Pancholi P, Carroll KC, Buchan BW, Chan RC, Dhiman N, Ford B, Granato PA, Harrington AT, Hernandez DR, Humphries RM, Jindra MR, Ledeboer NA, Miller SA, Mochon AB, Morgan MA, Patel R, Schreckenberger PC, Stamper PD, Simner PJ, Tucci NE, Zimmerman C, Wolk DM.

J Clin Microbiol. 2018 Mar 26;56(4). pii: e01329-17. doi: 10.1128/JCM.01329-17. Print 2018 Apr.


Effective utilization of C. difficile PCR and identification of clinicopathologic factors associated with conversion to a positive result in symptomatic patients.

Mostafa ME, Flynn T, Hartley CP, Ledeboer NA, Buchan BW.

Diagn Microbiol Infect Dis. 2018 Apr;90(4):307-310. doi: 10.1016/j.diagmicrobio.2017.11.021. Epub 2017 Dec 6.


Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization.

Faron ML, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2017 Dec;55(12):3328-3338. doi: 10.1128/JCM.00868-17. Epub 2017 Aug 30. Review.


Changing Diagnostic Paradigms for Microbiology: Report on an American Academy of Microbiology Colloquium held in Washington, DC, from 17 to 18 October 2016.

Dolen V, Bahk K, Carroll KC, Klugman K, Ledeboer NA, Miller MB.

Washington (DC): American Society for Microbiology; 2017.


Evaluation of Copan FecalSwab as Specimen Type for Use in Xpert C. difficile Assay.

Mashock MJ, Faron ML, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2017 Oct;55(10):3123-3129. doi: 10.1128/JCM.00369-17. Epub 2017 Aug 9.


A Novel Protocol Obviates Endoscope Sampling for Carbapenem-Resistant Enterobacteriaceae: Experience of a Center with a Prior Outbreak.

Smith ZL, Dua A, Saeian K, Ledeboer NA, Graham MB, Aburajab M, Ballard DD, Khan AH, Dua KS.

Dig Dis Sci. 2017 Nov;62(11):3100-3109. doi: 10.1007/s10620-017-4669-9. Epub 2017 Jul 5.


Impact of Organism Identification Method on Central Line-Associated Bloodstream Infection Designation.

Gomez EJ, Roberts AL, Robinson DP, Alby K, Ledeboer NA, Doern CD, Lander DW, Bearman G, Sullivan KV.

Infect Control Hosp Epidemiol. 2017 Aug;38(8):1007-1009. doi: 10.1017/ice.2017.112. Epub 2017 Jun 21. No abstract available.


Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinically Important Bacteria and Yeasts.

Wilson DA, Young S, Timm K, Novak-Weekley S, Marlowe EM, Madisen N, Lillie JL, Ledeboer NA, Smith R, Hyke J, Griego-Fullbright C, Jim P, Granato PA, Faron ML, Cumpio J, Buchan BW, Procop GW.

Am J Clin Pathol. 2017 Jun 1;147(6):623-631. doi: 10.1093/ajcp/aqw225.


Clinical Evaluation and Cost Analysis of Great Basin Shiga Toxin Direct Molecular Assay for Detection of Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Specimens.

Faron ML, Ledeboer NA, Connolly J, Granato PA, Alkins BR, Dien Bard J, Daly JA, Young S, Buchan BW.

J Clin Microbiol. 2017 Feb;55(2):519-525. doi: 10.1128/JCM.01939-16. Epub 2016 Dec 7.


Antimicrobial Curtains: Are They as Clean as You Think?

Sridhar SA, Ledeboer NA, Nanchal RS, Mackey T, Graham MB, VanDerSlik A, Silvia Munoz-Price L.

Infect Control Hosp Epidemiol. 2016 Oct;37(10):1260-2. doi: 10.1017/ice.2016.186. Epub 2016 Aug 17. No abstract available.


Automatic Digital Analysis of Chromogenic Media for Vancomycin-Resistant-Enterococcus Screens Using Copan WASPLab.

Faron ML, Buchan BW, Coon C, Liebregts T, van Bree A, Jansz AR, Soucy G, Korver J, Ledeboer NA.

J Clin Microbiol. 2016 Oct;54(10):2464-9. doi: 10.1128/JCM.01040-16. Epub 2016 Jul 13.


Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture.

Uphoff TS, Buchan BW, Ledeboer NA, Granato PA, Daly JA, Marti TN.

J Clin Microbiol. 2016 Sep;54(9):2388-90. doi: 10.1128/JCM.01268-16. Epub 2016 Jun 29.


Automation advances microbiology to operational excellence.

Ledeboer NA.

MLO Med Lab Obs. 2016 May;48(5):8-11; quiz 12. No abstract available.


Multicenter Evaluation of Meridian Bioscience HSV 1&2 Molecular Assay for Detection of Herpes Simplex Virus 1 and 2 from Clinical Cutaneous and Mucocutaneous Specimens.

Faron ML, Ledeboer NA, Patel A, Beqa SH, Yen-Lieberman B, Kohn D, Leber AL, Mayne D, Northern WI, Buchan BW.

J Clin Microbiol. 2016 Aug;54(8):2008-13. doi: 10.1128/JCM.00483-16. Epub 2016 May 18.


Resistance Mechanisms, Epidemiology, and Approaches to Screening for Vancomycin-Resistant Enterococcus in the Health Care Setting.

Faron ML, Ledeboer NA, Buchan BW.

J Clin Microbiol. 2016 Oct;54(10):2436-47. doi: 10.1128/JCM.00211-16. Epub 2016 May 4. Review.


Comparison of ESwab and Wound Fiber Swab Specimen Collection Devices for Use with Xpert SA Nasal Complete Assay.

Beck ET, Buchan BW, Reymann GC, Ledeboer NA.

J Clin Microbiol. 2016 Jul;54(7):1904-1906. doi: 10.1128/JCM.00449-16. Epub 2016 Apr 27.


Is Staphylococcal Screening and Suppression an Effective Interventional Strategy for Reduction of Surgical Site Infection?

Edmiston CE Jr, Ledeboer NA, Buchan BW, Spencer M, Seabrook GR, Leaper D.

Surg Infect (Larchmt). 2016 Apr;17(2):158-66. doi: 10.1089/sur.2015.257. Epub 2016 Feb 2. Review.


Automated Scoring of Chromogenic Media for Detection of Methicillin-Resistant Staphylococcus aureus by Use of WASPLab Image Analysis Software.

Faron ML, Buchan BW, Vismara C, Lacchini C, Bielli A, Gesu G, Liebregts T, van Bree A, Jansz A, Soucy G, Korver J, Ledeboer NA.

J Clin Microbiol. 2016 Mar;54(3):620-4. doi: 10.1128/JCM.02778-15. Epub 2015 Dec 30.


Multicenter Evaluation of MRSASelect II Chromogenic Agar for Identification of Methicillin-Resistant Staphylococcus aureus from Wound and Nasal Specimens.

Hernandez DR, Newton DW, Ledeboer NA, Buchan B, Young C, Clark AE, Connoly J, Wolk DM.

J Clin Microbiol. 2016 Feb;54(2):305-11. doi: 10.1128/JCM.02410-15. Epub 2015 Nov 18.


Multicenter Evaluation of the Xpert Norovirus Assay for Detection of Norovirus Genogroups I and II in Fecal Specimens.

Gonzalez MD, Langley LC, Buchan BW, Faron ML, Maier M, Templeton K, Walker K, Popowitch EB, Miller MB, Rao A, Liebert UG, Ledeboer NA, Vinjé J, Burnham CA.

J Clin Microbiol. 2016 Jan;54(1):142-7. doi: 10.1128/JCM.02361-15. Epub 2015 Nov 11.


Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinical Aerobic Gram-Negative Bacterial Isolates.

Faron ML, Buchan BW, Hyke J, Madisen N, Lillie JL, Granato PA, Wilson DA, Procop GW, Novak-Weekley S, Marlowe E, Cumpio J, Griego-Fullbright C, Kindig S, Timm K, Young S, Ledeboer NA.

PLoS One. 2015 Nov 3;10(11):e0141350. doi: 10.1371/journal.pone.0141350. eCollection 2015.


Comparative Evaluation of AmpliVue HSV 1+2 Assay with ELVIS Culture for Detecting Herpes Simplex Virus 1 (HSV-1) and HSV-2 in Clinical Specimens.

Granato PA, Alkins BR, Yen-Lieberman B, Greene WH, Connolly J, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2015 Dec;53(12):3922-5. doi: 10.1128/JCM.01905-15. Epub 2015 Oct 14.


Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay.

Ledeboer NA, Lopansri BK, Dhiman N, Cavagnolo R, Carroll KC, Granato P, Thomson R Jr, Butler-Wu SM, Berger H, Samuel L, Pancholi P, Swyers L, Hansen GT, Tran NK, Polage CR, Thomson KS, Hanson ND, Winegar R, Buchan BW.

J Clin Microbiol. 2015 Aug;53(8):2460-72. doi: 10.1128/JCM.00581-15. Epub 2015 May 20.


Detection of multi-drug resistant Escherichia coli in the urban waterways of Milwaukee, WI.

Kappell AD, DeNies MS, Ahuja NH, Ledeboer NA, Newton RJ, Hristova KR.

Front Microbiol. 2015 Apr 29;6:336. doi: 10.3389/fmicb.2015.00336. eCollection 2015.


Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay.

Faron ML, Ledeboer NA, Granato P, Daly JA, Pierce K, Pancholi P, Uphoff TS, Buchan BW.

J Clin Microbiol. 2015 Jul;53(7):2365-7. doi: 10.1128/JCM.01085-15. Epub 2015 May 13.


Comparison of the next-generation Xpert MRSA/SA BC assay and the GeneOhm StaphSR assay to routine culture for identification of Staphylococcus aureus and methicillin-resistant S. aureus in positive-blood-culture broths.

Buchan BW, Allen S, Burnham CA, McElvania TeKippe E, Davis T, Levi M, Mayne D, Pancholi P, Relich RF, Thomson R, Ledeboer NA.

J Clin Microbiol. 2015 Mar;53(3):804-9. doi: 10.1128/JCM.03108-14. Epub 2014 Dec 24.


Multicenter clinical evaluation of the Xpert GBS LB assay for detection of group B Streptococcus in prenatal screening specimens.

Buchan BW, Faron ML, Fuller D, Davis TE, Mayne D, Ledeboer NA.

J Clin Microbiol. 2015 Feb;53(2):443-8. doi: 10.1128/JCM.02598-14. Epub 2014 Nov 19.


Emerging technologies for the clinical microbiology laboratory.

Buchan BW, Ledeboer NA.

Clin Microbiol Rev. 2014 Oct;27(4):783-822. doi: 10.1128/CMR.00003-14. Review.


Antimicrobial activity of ceftaroline and other anti-infective agents against microbial pathogens recovered from the surgical intensive care patient population: a prevalence analysis.

Edmiston CE Jr, Krepel CJ, Leaper D, Ledeboer NA, Mackey TL, Graham MB, Lee C, Rossi PJ, Brown KR, Lewis BD, Seabrook GR.

Surg Infect (Larchmt). 2014 Dec;15(6):745-51. doi: 10.1089/sur.2013.172.


Multicenter evaluation of the Quidel Lyra Direct C. difficile nucleic acid amplification assay.

Beck ET, Buchan BW, Riebe KM, Alkins BR, Pancholi P, Granato PA, Ledeboer NA.

J Clin Microbiol. 2014 Jun;52(6):1998-2002. doi: 10.1128/JCM.03089-13. Epub 2014 Mar 26.


The automated clinical microbiology laboratory: fact or fantasy?

Ledeboer NA, Dallas SD.

J Clin Microbiol. 2014 Sep;52(9):3140-6. doi: 10.1128/JCM.00686-14. Epub 2014 Mar 19.


Clinical evaluation of the walk-away specimen processor and ESwab for recovery of Streptococcus agalactiae isolates in prenatal screening specimens.

Buchan BW, Olson WJ, Mackey TL, Ledeboer NA.

J Clin Microbiol. 2014 Jun;52(6):2166-8. doi: 10.1128/JCM.00374-14. Epub 2014 Mar 12.


Photo quiz: a 5-year-old male with multiple pustules covering the entire scalp. Inflammatory Tinea Capitis.

Feih J, Ledeboer NA, Peppard WJ.

J Clin Microbiol. 2014 Apr;52(4):1027, 1312. doi: 10.1128/JCM.00020-13. No abstract available.


ESwab as an optional collection device for use with the Affirm VPIII microbial test system.

Rivers CA, Lee JY, Sharples N, Ledeboer NA, Schwebke JR.

J Clin Microbiol. 2014 May;52(5):1698-700. doi: 10.1128/JCM.00048-14. Epub 2014 Feb 12.


Comparison of MALDI-TOF MS with HPLC and nucleic acid sequencing for the identification of Mycobacterium species in cultures using solid medium and broth.

Buchan BW, Riebe KM, Timke M, Kostrzewa M, Ledeboer NA.

Am J Clin Pathol. 2014 Jan;141(1):25-34. doi: 10.1309/AJCPBPUBUDEW2OAG.


Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus.

Anderson NW, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2014 Jan;52(1):2-8. doi: 10.1128/JCM.01966-13. Epub 2013 Oct 16. Review.


Multicenter evaluation of the Verigene Clostridium difficile nucleic acid assay.

Carroll KC, Buchan BW, Tan S, Stamper PD, Riebe KM, Pancholi P, Kelly C, Rao A, Fader R, Cavagnolo R, Watson W, Goering RV, Trevino EA, Weissfeld AS, Ledeboer NA.

J Clin Microbiol. 2013 Dec;51(12):4120-5. doi: 10.1128/JCM.01690-13. Epub 2013 Oct 2.


Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and shiga toxin-producing Escherichia coli isolates in stool specimens.

Buchan BW, Olson WJ, Pezewski M, Marcon MJ, Novicki T, Uphoff TS, Chandramohan L, Revell P, Ledeboer NA.

J Clin Microbiol. 2013 Dec;51(12):4001-7. doi: 10.1128/JCM.02056-13. Epub 2013 Sep 18.


Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test.

Buchan BW, Ginocchio CC, Manii R, Cavagnolo R, Pancholi P, Swyers L, Thomson RB Jr, Anderson C, Kaul K, Ledeboer NA.

PLoS Med. 2013;10(7):e1001478. doi: 10.1371/journal.pmed.1001478. Epub 2013 Jul 2.


Multicenter clinical evaluation of VRESelect agar for identification of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium.

Anderson NW, Buchan BW, Young CL, Newton DW, Brenke C, Lapsley L, Granato PA, Ledeboer NA.

J Clin Microbiol. 2013 Aug;51(8):2758-60. doi: 10.1128/JCM.00979-13. Epub 2013 Jun 12.

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