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J Glob Antimicrob Resist. 2017 Jun;9:10-14. doi: 10.1016/j.jgar.2016.12.015. Epub 2017 Mar 9.

Detection of carbapenemase-producers: Evaluating the performance of the carbapenem inactivation method and Carba NP test versus multiplex PCR.

Author information

1
Microbiology and Immunology Department, Faculty of Medicine, Cairo University, 1 El-Saraya St., Kasr Al Ainy, Cairo, Egypt. Electronic address: m_l@kasralainy.edu.eg.
2
Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

Abstract

OBJECTIVES:

With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance of the carbapenem inactivation method (CIM) and the Carba NP test in the detection of carbapenemase-producers.

METHODS:

A panel of 203 Gram-negative bacterial isolates screened for carbapenem resistance were subjected to the CIM and Carba NP test. The results were compared with multiplex PCR targeting various carbapenemase genes.

RESULTS:

According to multiplex PCR, 92 (45.3%) of 203 isolates were found to harbour one or more carbapenemase genes, with blaNDM and blaKPC being the most commonly encountered. The sensitivity and specificity of the CIM were 95.7% and 95.5% respectively, whilst those of the Carba NP test were 75.0% and 99.1%, respectively. Both methods were found to be rapid and reliable in the detection of carbapenemases and showed a high agreement with multiplex PCR.

CONCLUSIONS:

As the list of carbapenemase genes continues to expand, the reliability of PCR has become doubtful; hence, the CIM and Carba NP test could offer promising alternatives, with the CIM being of a lower cost and less labour intensive.

KEYWORDS:

CIM; Carba NP; Carbapenemases; Enterobacteriaceae; Pseudomonas

PMID:
28286140
DOI:
10.1016/j.jgar.2016.12.015

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