Format
Sort by
Items per page

Send to

Choose Destination

Search results

Items: 35

1.

Advancing Biologics Development Programs with Legacy Cell Lines: Advantages and Limitations of Genetic Testing for Addressing Clonality Concerns Prior to Availability of Late Stage Process and Product Consistency Data.

Wu P, Hartman T, Almond L, Stevens J, Thrift J, OJha J, Alves C, Shaw D, Laird MW, Emmins R, Zhu Y, Liu R, Du Z, Koehler R, Jostock T, Anderson K, Campbell C, Clarke H.

PDA J Pharm Sci Technol. 2019 Sep 13. pii: pdajpst.2018.009316. doi: 10.5731/pdajpst.2018.009316. [Epub ahead of print]

PMID:
31519780
2.

Pyruvate Kinase Muscle-1 Expression Appears to Drive Lactogenic Behavior in CHO Cell Lines, Triggering Lower Viability and Productivity: A Case Study.

Tang D, Subramanian J, Haley B, Baker J, Luo L, Hsu W, Liu P, Sandoval W, Laird MW, Snedecor B, Shiratori M, Misaghi S.

Biotechnol J. 2019 Apr;14(4):e1800332. doi: 10.1002/biot.201800332. Epub 2018 Sep 16.

PMID:
30179303
3.

Implementation of Plate Imaging for Demonstration of Monoclonality in Biologics Manufacturing Development.

Zingaro K, Shaw D, Carson J, Mayer-Bartschmid A, Bender C, Alves C, Mcvey D, Qian NX, Wei Q, Laird MW, Zhu Y, Emmins R, Follit JA, Porter A, Racher AJ, Milne S, Carubelli I, Du Z, Khatri A, Failly M, Broly H, Lee F, Reeser M, Spidel J, Anderson K, Demaria C, Di-Carlo J, Gill J, Lundquist A, Kumar SR, Gill T.

PDA J Pharm Sci Technol. 2018 Jul-Aug;72(4):438-450. doi: 10.5731/pdajpst.2018.008789. Epub 2018 Apr 18.

PMID:
29669815
4.

Strain engineering to reduce acetate accumulation during microaerobic growth conditions in Escherichia coli.

Veeravalli K, Schindler T, Dong E, Yamada M, Hamilton R, Laird MW.

Biotechnol Prog. 2018 Mar;34(2):303-314. doi: 10.1002/btpr.2592. Epub 2017 Dec 14.

PMID:
29193870
5.

Development and characterization of an automated imaging workflow to generate clonally-derived cell lines for therapeutic proteins.

Shaw D, Yim M, Tsukuda J, Joly JC, Lin A, Snedecor B, Laird MW, Lang SE.

Biotechnol Prog. 2018 May;34(3):584-592. doi: 10.1002/btpr.2561. Epub 2017 Oct 10.

PMID:
28960825
6.

Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally-derived or not.

Zhou Y, Shaw D, Lam C, Tsukuda J, Yim M, Tang D, Louie S, Laird MW, Snedecor B, Misaghi S.

Biotechnol Prog. 2018 May;34(3):559-569. doi: 10.1002/btpr.2560. Epub 2017 Oct 9.

PMID:
28941017
7.

A strategy to accelerate protein production from a pool of clones in Chinese hamster ovary cells for toxicology studies.

Hu Z, Hsu W, Pynn A, Ng D, Quicho D, Adem Y, Kwong Z, Mauger B, Joly J, Snedecor B, Laird MW, Andersen DC, Shen A.

Biotechnol Prog. 2017 Nov;33(6):1449-1455. doi: 10.1002/btpr.2467. Epub 2017 Apr 26.

PMID:
28371489
8.

Evaluation of heavy chain C-terminal deletions on productivity and product quality of monoclonal antibodies in Chinese hamster ovary (CHO) cells.

Hu Z, Tang D, Misaghi S, Jiang G, Yu C, Yim M, Shaw D, Snedecor B, Laird MW, Shen A.

Biotechnol Prog. 2017 May;33(3):786-794. doi: 10.1002/btpr.2444. Epub 2017 Mar 24.

PMID:
28188688
9.

Toward an era of utilizing methionine overproducing hosts for recombinant protein production in Escherichia coli.

Veeravalli K, Laird MW.

Bioengineered. 2015;6(3):132-5. doi: 10.1080/21655979.2015.1030544. Epub 2015 Mar 24.

10.

Air sparging for prevention of antibody disulfide bond reduction in harvested CHO cell culture fluid.

Mun M, Khoo S, Do Minh A, Dvornicky J, Trexler-Schmidt M, Kao YH, Laird MW.

Biotechnol Bioeng. 2015 Apr;112(4):734-42. doi: 10.1002/bit.25495. Epub 2014 Dec 23.

PMID:
25384896
11.

Strain engineering to prevent norleucine incorporation during recombinant protein production in Escherichia coli.

Veeravalli K, Laird MW, Fedesco M, Zhang Y, Yu XC.

Biotechnol Prog. 2015 Jan-Feb;31(1):204-11. doi: 10.1002/btpr.1999. Epub 2014 Oct 21.

PMID:
25315437
12.

Effect of individual Fc methionine oxidation on FcRn binding: Met252 oxidation impairs FcRn binding more profoundly than Met428 oxidation.

Gao X, Ji JA, Veeravalli K, Wang YJ, Zhang T, Mcgreevy W, Zheng K, Kelley RF, Laird MW, Liu J, Cromwell M.

J Pharm Sci. 2015 Feb;104(2):368-77. doi: 10.1002/jps.24136. Epub 2014 Aug 29.

PMID:
25175600
13.

Eliminating tyrosine sequence variants in CHO cell lines producing recombinant monoclonal antibodies.

Feeney L, Carvalhal V, Yu XC, Chan B, Michels DA, Wang YJ, Shen A, Ressl J, Dusel B, Laird MW.

Biotechnol Bioeng. 2013 Apr;110(4):1087-97. doi: 10.1002/bit.24759. Epub 2013 Feb 15.

PMID:
23108857
14.

Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.

Koterba KL, Borgschulte T, Laird MW.

J Biotechnol. 2012 Jan;157(1):261-7. doi: 10.1016/j.jbiotec.2011.11.009. Epub 2011 Nov 25.

PMID:
22138638
15.

Characterization of a nutrient feed precipitate from an E. coli fermentation process.

Speciner L, Mallon E, Leung S, Laird MW, Esue O.

Biotechnol Prog. 2010 Sep-Oct;26(5):1290-4. doi: 10.1002/btpr.419.

PMID:
20945485
16.

Use of disposable reactors to generate inoculum cultures for E. coli production fermentations.

Mahajan E, Matthews T, Hamilton R, Laird MW.

Biotechnol Prog. 2010 Jul-Aug;26(4):1200-3. doi: 10.1002/btpr.414.

PMID:
20730774
17.

Mechanism of antibody reduction in cell culture production processes.

Kao YH, Hewitt DP, Trexler-Schmidt M, Laird MW.

Biotechnol Bioeng. 2010 Nov 1;107(4):622-32. doi: 10.1002/bit.22848.

PMID:
20589844
18.

Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells.

Guo D, Gao A, Michels DA, Feeney L, Eng M, Chan B, Laird MW, Zhang B, Yu XC, Joly J, Snedecor B, Shen A.

Biotechnol Bioeng. 2010 Sep 1;107(1):163-71. doi: 10.1002/bit.22780.

PMID:
20506532
19.

High-yield expression of human vascular endothelial growth factor VEGF(165) in Escherichia coli and purification for therapeutic applications.

Pizarro SA, Gunson J, Field MJ, Dinges R, Khoo S, Dalal M, Lee M, Kaleas KA, Moiseff K, Garnick S, Reilly DE, Laird MW, Schmelzer CH.

Protein Expr Purif. 2010 Aug;72(2):184-93. doi: 10.1016/j.pep.2010.03.007. Epub 2010 Mar 17.

PMID:
20302942
20.

Characterization of a monoclonal antibody cell culture production process using a quality by design approach.

Horvath B, Mun M, Laird MW.

Mol Biotechnol. 2010 Jul;45(3):203-6. doi: 10.1007/s12033-010-9267-4.

PMID:
20300882
21.

Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing.

Trexler-Schmidt M, Sargis S, Chiu J, Sze-Khoo S, Mun M, Kao YH, Laird MW.

Biotechnol Bioeng. 2010 Jun 15;106(3):452-61. doi: 10.1002/bit.22699.

PMID:
20178122
22.

Structural and functional characterization of CC chemokine CCL14.

Blain KY, Kwiatkowski W, Zhao Q, La Fleur D, Naik C, Chun TW, Tsareva T, Kanakaraj P, Laird MW, Shah R, George L, Sanyal I, Moore PA, Demeler B, Choe S.

Biochemistry. 2007 Sep 4;46(35):10008-15. Epub 2007 Aug 11.

PMID:
17691823
23.

Fluid support worsens outcome and negates the benefit of protective antigen-directed monoclonal antibody in a lethal toxin-infused rat Bacillus anthracis shock model.

Sherer K, Li Y, Cui X, Li X, Subramanian M, Laird MW, Moayeri M, Leppla SH, Fitz Y, Su J, Eichacker PQ.

Crit Care Med. 2007 Jun;35(6):1560-7.

PMID:
17452924
24.

Bacillus anthracis edema and lethal toxin have different hemodynamic effects but function together to worsen shock and outcome in a rat model.

Cui X, Li Y, Li X, Laird MW, Subramanian M, Moayeri M, Leppla SH, Fitz Y, Su J, Sherer K, Eichacker PQ.

J Infect Dis. 2007 Feb 15;195(4):572-80. Epub 2007 Jan 3.

PMID:
17230417
25.

Scalable purification of Bacillus anthracis protective antigen from Escherichia coli.

Gwinn W, Zhang M, Mon S, Sampey D, Zukauskas D, Kassebaum C, Zmuda JF, Tsai A, Laird MW.

Protein Expr Purif. 2006 Jan;45(1):30-6.

PMID:
15935696
26.

Development of an edema factor-mediated cAMP-induction bioassay for detecting antibody-mediated neutralization of anthrax protective antigen.

Zmuda JF, Zhang L, Richards T, Pham Q, Zukauskas D, Pierre JL, Laird MW, Askins J, Choi GH.

J Immunol Methods. 2005 Mar;298(1-2):47-60.

PMID:
15847796
27.

Heterologous protein production from the inducible MET25 promoter in Saccharomyces cerevisiae.

Solow SP, Sengbusch J, Laird MW.

Biotechnol Prog. 2005 Mar-Apr;21(2):617-20.

PMID:
15801808
28.

Optimization of BLyS production and purification from Escherichia coli.

Laird MW, Sampey GC, Johnson K, Zukauskas D, Pierre J, Hong JS, Cooksey BA, Li Y, Galperina O, Karwoski JD, Burke RN.

Protein Expr Purif. 2005 Feb;39(2):237-46.

PMID:
15642475
29.

Production of biologically active Bacillus anthracis edema factor in Escherichia coli.

Cooksey BA, Sampey GC, Pierre JL, Zhang X, Karwoski JD, Choi GH, Laird MW.

Biotechnol Prog. 2004 Nov-Dec;20(6):1651-9.

PMID:
15575695
30.

Production and purification of Bacillus anthracis protective antigen from Escherichia coli.

Laird MW, Zukauskas D, Johnson K, Sampey GC, Olsen H, Garcia A, Karwoski JD, Cooksey BA, Choi GH, Askins J, Tsai A, Pierre J, Gwinn W.

Protein Expr Purif. 2004 Nov;38(1):145-52.

PMID:
15477093
31.

Keratinocyte growth factor-2 production in an ompT-deficient Escherichia coli K-12 mutant.

Laird MW, Cope K, Atkinson R, Donahoe M, Johnson K, Melick M.

Biotechnol Prog. 2004 Jan-Feb;20(1):44-50.

PMID:
14763822
32.

The cryptic ushA gene (ushA(c)) in natural isolates of Salmonella enterica (serotype Typhimurium) has been inactivated by a single missense mutation.

Innes D, Beacham IR, Beven CA, Douglas M, Laird MW, Joly JC, Burns DM.

Microbiology. 2001 Jul;147(Pt 7):1887-96.

PMID:
11429465
33.

Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response.

Raivio TL, Laird MW, Joly JC, Silhavy TJ.

Mol Microbiol. 2000 Sep;37(5):1186-97.

34.
35.

Supplemental Content

Loading ...
Support Center