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Antimicrob Agents Chemother. 2018 Oct 24;62(11). pii: e01281-18. doi: 10.1128/AAC.01281-18. Print 2018 Nov.

Carbapenem Resistance Caused by High-Level Expression of OXA-663 β-Lactamase in an OmpK36-Deficient Klebsiella pneumoniae Clinical Isolate.

Ma P1,2,3, Laibinis HH1,2, Ernst CM1,2,3, Hung DT4,2,3.

Author information

1
The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
2
Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.
3
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
4
The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA hung@molbio.mgh.harvard.edu.

Abstract

Carbapenem resistance is mainly mediated by carbapenemases or extended-spectrum β-lactamases (ESBL) plus a loss of porins. However, we have identified a Klebsiella pneumoniae clinical isolate that contains neither carbapenemases nor ESBLs. Instead, we found that high-level expression of a novel bla OXA-10-derived β-lactamase gene, bla OXA-663, in conjunction with OmpK36 deficiency results in high-level carbapenem resistance. This finding demonstrates the combinatorial complexity of factors, including β-lactamase activity, its expression levels, and porin activity, that yield carbapenem resistance.

KEYWORDS:

Klebsiella; OXA-663; carbapenem resistance; β-lactamases

PMID:
30126962
PMCID:
PMC6201135
[Available on 2019-04-24]
DOI:
10.1128/AAC.01281-18

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