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Items: 1 to 50 of 133

1.

Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells.

Hennicke J, Kunert R.

Methods Mol Biol. 2020;2095:295-302. doi: 10.1007/978-1-0716-0191-4_17.

PMID:
31858475
2.

Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment.

Mayrhofer P, Kunert R.

Methods Mol Biol. 2020;2095:27-39. doi: 10.1007/978-1-0716-0191-4_3.

PMID:
31858461
3.

Rapid development of clone-specific, high-performing perfusion media from established feed supplements.

Mayrhofer P, Reinhart D, Castan A, Kunert R.

Biotechnol Prog. 2019 Nov 3:e2933. doi: 10.1002/btpr.2933. [Epub ahead of print]

PMID:
31680446
4.

Germinality does not necessarily define mAb expression and thermal stability.

Schwaigerlehner L, Mayrhofer P, Diem M, Steinfellner W, Fenech E, Oostenbrink C, Kunert R.

Appl Microbiol Biotechnol. 2019 Sep;103(18):7505-7518. doi: 10.1007/s00253-019-09998-3. Epub 2019 Jul 26.

5.

Stable M(II)-Radicals and Nickel(III) Complexes of a Bis(phenol) N-Heterocyclic Carbene Chelated to Group 10 Metal Ions.

Kunert R, Philouze C, Jarjayes O, Thomas F.

Inorg Chem. 2019 Jun 17;58(12):8030-8044. doi: 10.1021/acs.inorgchem.9b00784. Epub 2019 Jun 4.

PMID:
31185559
6.

Impact of temperature and pH on recombinant human IgM quality attributes and productivity.

Hennicke J, Reinhart D, Altmann F, Kunert R.

N Biotechnol. 2019 May 25;50:20-26. doi: 10.1016/j.nbt.2019.01.001. Epub 2019 Jan 7.

7.

Comparative Antigenicity of Thiourea and Adipic Amide Linked Neoglycoconjugates Containing Modified Oligomannose Epitopes for the Carbohydrate-Specific anti-HIV Antibody 2G12.

Trattnig N, Mayrhofer P, Kunert R, Mach L, Pantophlet R, Kosma P.

Bioconjug Chem. 2019 Jan 16;30(1):70-82. doi: 10.1021/acs.bioconjchem.8b00731. Epub 2018 Dec 28.

8.

Differential gene expression of a feed-spiked super-producing CHO cell line.

Reinhart D, Damjanovic L, Castan A, Ernst W, Kunert R.

J Biotechnol. 2018 Nov 10;285:23-37. doi: 10.1016/j.jbiotec.2018.08.013. Epub 2018 Aug 26.

9.

Nomenclature of humanized mAbs: Early concepts, current challenges and future perspectives.

Mayrhofer P, Kunert R.

Hum Antibodies. 2019;27(1):37-51. doi: 10.3233/HAB-180347. Review.

10.

Lessons learned from merging wet lab experiments with molecular simulation to improve mAb humanization.

Schwaigerlehner L, Pechlaner M, Mayrhofer P, Oostenbrink C, Kunert R.

Protein Eng Des Sel. 2018 Jul 1;31(7-8):257-265. doi: 10.1093/protein/gzy009.

11.

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis.

Reinhart D, Damjanovic L, Kaisermayer C, Sommeregger W, Gili A, Gasselhuber B, Castan A, Mayrhofer P, Grünwald-Gruber C, Kunert R.

Biotechnol J. 2019 Mar;14(3):e1700686. doi: 10.1002/biot.201700686. Epub 2018 Jun 11.

PMID:
29701329
12.

Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control.

Albrecht S, Kaisermayer C, Reinhart D, Ambrose M, Kunert R, Lindeberg A, Bones J.

Anal Bioanal Chem. 2018 May;410(13):3197-3207. doi: 10.1007/s00216-018-1029-3. Epub 2018 Apr 2.

PMID:
29607450
13.

Glycan profile of CHO derived IgM purified by highly efficient single step affinity chromatography.

Hennicke J, Lastin AM, Reinhart D, Grünwald-Gruber C, Altmann F, Kunert R.

Anal Biochem. 2017 Dec 15;539:162-166. doi: 10.1016/j.ab.2017.10.020. Epub 2017 Nov 4.

PMID:
29106908
14.

Repetitive transcranial magnetic stimulation over inferior frontal cortex impairs the suppression (but not expression) of action impulses during action conflict.

van Campen AD, Kunert R, van den Wildenberg WPM, Ridderinkhof KR.

Psychophysiology. 2018 Mar;55(3). doi: 10.1111/psyp.13003. Epub 2017 Sep 20.

PMID:
28929571
15.

A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples.

Battin C, Hennig A, Mayrhofer P, Kunert R, Zlabinger GJ, Steinberger P, Paster W.

PLoS One. 2017 May 24;12(5):e0178220. doi: 10.1371/journal.pone.0178220. eCollection 2017.

16.

Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression.

Mayrhofer P, Kunert R.

Methods Mol Biol. 2017;1603:57-69. doi: 10.1007/978-1-4939-6972-2_4.

PMID:
28493123
17.

Entrainment to an auditory signal: Is attention involved?

Kunert R, Jongman SR.

J Exp Psychol Gen. 2017 Jan;146(1):77-88. doi: 10.1037/xge0000246.

PMID:
28054814
18.

An Independent Psychometric Evaluation of the PROMS Measure of Music Perception Skills.

Kunert R, Willems RM, Hagoort P.

PLoS One. 2016 Jul 11;11(7):e0159103. doi: 10.1371/journal.pone.0159103. eCollection 2016.

19.
20.

Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression.

Kaisermayer C, Reinhart D, Gili A, Chang M, Aberg PM, Castan A, Kunert R.

J Biotechnol. 2016 Jun 10;227:3-9. doi: 10.1016/j.jbiotec.2016.03.054. Epub 2016 Apr 2.

21.

Language influences music harmony perception: effects of shared syntactic integration resources beyond attention.

Kunert R, Willems RM, Hagoort P.

R Soc Open Sci. 2016 Feb 3;3(2):150685. doi: 10.1098/rsos.150685. eCollection 2016 Feb.

22.

Advances in recombinant antibody manufacturing.

Kunert R, Reinhart D.

Appl Microbiol Biotechnol. 2016 Apr;100(8):3451-61. doi: 10.1007/s00253-016-7388-9. Epub 2016 Mar 3. Review.

23.

Proteomic differences in recombinant CHO cells producing two similar antibody fragments.

Sommeregger W, Mayrhofer P, Steinfellner W, Reinhart D, Henry M, Clynes M, Meleady P, Kunert R.

Biotechnol Bioeng. 2016 Sep;113(9):1902-12. doi: 10.1002/bit.25957. Epub 2016 Mar 16.

24.

Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations.

Margreitter C, Mayrhofer P, Kunert R, Oostenbrink C.

J Mol Recognit. 2016 Jun;29(6):266-75. doi: 10.1002/jmr.2527. Epub 2016 Jan 8.

25.

Music and Language Syntax Interact in Broca's Area: An fMRI Study.

Kunert R, Willems RM, Casasanto D, Patel AD, Hagoort P.

PLoS One. 2015 Nov 4;10(11):e0141069. doi: 10.1371/journal.pone.0141069. eCollection 2015.

26.

A Commentary on: "Neural overlap in processing music and speech".

Kunert R, Slevc LR.

Front Hum Neurosci. 2015 Jun 3;9:330. doi: 10.3389/fnhum.2015.00330. eCollection 2015. No abstract available.

27.

Heterologous protein production using euchromatin-containing expression vectors in mammalian cells.

Zboray K, Sommeregger W, Bogner E, Gili A, Sterovsky T, Fauland K, Grabner B, Stiedl P, Moll HP, Bauer A, Kunert R, Casanova E.

Nucleic Acids Res. 2015 Sep 18;43(16):e102. doi: 10.1093/nar/gkv475. Epub 2015 May 14.

28.

Benchmarking of commercially available CHO cell culture media for antibody production.

Reinhart D, Damjanovic L, Kaisermayer C, Kunert R.

Appl Microbiol Biotechnol. 2015 Jun;99(11):4645-57. doi: 10.1007/s00253-015-6514-4. Epub 2015 Apr 7.

29.

Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

Mayerhofer B, Stöger A, Pietzka AT, Fernandez HL, Prewein B, Sorschag S, Kunert R, Allerberger F, Ruppitsch W.

PLoS One. 2015 Mar 13;10(3):e0116713. doi: 10.1371/journal.pone.0116713. eCollection 2015.

30.

Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM.

Chromikova V, Mader A, Hofbauer S, Göbl C, Madl T, Gach JS, Bauernfried S, Furtmüller PG, Forthal DN, Mach L, Obinger C, Kunert R.

Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1536-44. doi: 10.1016/j.bbapap.2015.02.018. Epub 2015 Mar 5.

31.

Speed and accuracy of dyslexic versus typical word recognition: an eye-movement investigation.

Kunert R, Scheepers C.

Front Psychol. 2014 Oct 9;5:1129. doi: 10.3389/fpsyg.2014.01129. eCollection 2014.

32.

Upstream and downstream processing of recombinant IgA.

Reinhart D, Kunert R.

Biotechnol Lett. 2015 Feb;37(2):241-51. doi: 10.1007/s10529-014-1686-z. Epub 2014 Sep 26. Review.

PMID:
25257601
33.

Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

Mayrhofer P, Kratzer B, Sommeregger W, Steinfellner W, Reinhart D, Mader A, Turan S, Qiao J, Bode J, Kunert R.

Appl Microbiol Biotechnol. 2014 Dec;98(23):9723-33. doi: 10.1007/s00253-014-6011-1. Epub 2014 Aug 27.

34.

Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.

Maccani A, Hackl M, Leitner C, Steinfellner W, Graf AB, Tatto NE, Karbiener M, Scheideler M, Grillari J, Mattanovich D, Kunert R, Borth N, Grabherr R, Ernst W.

Appl Microbiol Biotechnol. 2014 Sep;98(17):7535-48. doi: 10.1007/s00253-014-5911-4. Epub 2014 Jul 23.

35.

Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients.

Singer J, Fazekas J, Wang W, Weichselbaumer M, Matz M, Mader A, Steinfellner W, Meitz S, Mechtcheriakova D, Sobanov Y, Willmann M, Stockner T, Spillner E, Kunert R, Jensen-Jarolim E.

Mol Cancer Ther. 2014 Jul;13(7):1777-1790. doi: 10.1158/1535-7163.MCT-13-0288. Epub 2014 Apr 22.

36.

Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

Chromikova V, Mader A, Steinfellner W, Kunert R.

Cytotechnology. 2015 Mar;67(2):343-56. doi: 10.1007/s10616-014-9693-4. Epub 2014 Mar 11.

37.

In search of expression bottlenecks in recombinant CHO cell lines--a case study.

Reinhart D, Sommeregger W, Debreczeny M, Gludovacz E, Kunert R.

Appl Microbiol Biotechnol. 2014 Jul;98(13):5959-65. doi: 10.1007/s00253-014-5584-z. Epub 2014 Feb 21.

PMID:
24557570
38.

Novel human renal proximal tubular cell line for the production of complex proteins.

Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R.

J Biotechnol. 2014 Apr 20;176:29-39. doi: 10.1016/j.jbiotec.2014.02.001. Epub 2014 Feb 16.

PMID:
24553072
39.

Novel bifunctional single-chain variable antibody fragments to enhance virolysis by complement: generation and proof-of-concept.

Huber G, Bánki Z, Kunert R, Stoiber H.

Biomed Res Int. 2014;2014:971345. doi: 10.1155/2014/971345. Epub 2014 Jan 12.

40.

Stable overexpression of miR-17 enhances recombinant protein production of CHO cells.

Jadhav V, Hackl M, Klanert G, Hernandez Bort JA, Kunert R, Grillari J, Borth N.

J Biotechnol. 2014 Apr 10;175:38-44. doi: 10.1016/j.jbiotec.2014.01.032. Epub 2014 Feb 8.

41.

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins.

Maccani A, Landes N, Stadlmayr G, Maresch D, Leitner C, Maurer M, Gasser B, Ernst W, Kunert R, Mattanovich D.

Biotechnol J. 2014 Apr;9(4):526-37. doi: 10.1002/biot.201300305. Epub 2014 Jan 27.

42.

Ch14.18 antibody produced in CHO cells in relapsed or refractory Stage 4 neuroblastoma patients: a SIOPEN Phase 1 study.

Ladenstein R, Weixler S, Baykan B, Bleeke M, Kunert R, Katinger D, Pribill I, Glander P, Bauer S, Pistoia V, Michon J, Garaventa A, Lode HN.

MAbs. 2013 Sep-Oct;5(5):801-9. doi: 10.4161/mabs.25215. Epub 2013 May 31.

43.

Transgene copy number comparison in recombinant mammalian cell lines: critical reflection of quantitative real-time PCR evaluation.

Sommeregger W, Prewein B, Reinhart D, Mader A, Kunert R.

Cytotechnology. 2013 Oct;65(5):811-8. doi: 10.1007/s10616-013-9606-y. Epub 2013 Jun 27.

44.

Recent advances in recombinant protein production: BAC-based expression vectors, the bigger the better.

Kunert R, Casanova E.

Bioengineered. 2013 Jul-Aug;4(4):258-61. doi: 10.4161/bioe.24060. Epub 2013 Jul 2.

45.

Recognition of membrane-bound fusion-peptide/MPER complexes by the HIV-1 neutralizing 2F5 antibody: implications for anti-2F5 immunogenicity.

Huarte N, Araujo A, Arranz R, Lorizate M, Quendler H, Kunert R, Valpuesta JM, Nieva JL.

PLoS One. 2012;7(12):e52740. doi: 10.1371/journal.pone.0052740. Epub 2012 Dec 21.

46.

Exploration of BAC versus plasmid expression vectors in recombinant CHO cells.

Mader A, Prewein B, Zboray K, Casanova E, Kunert R.

Appl Microbiol Biotechnol. 2013 May;97(9):4049-54. doi: 10.1007/s00253-012-4498-x. Epub 2012 Oct 19.

PMID:
23081777
47.

Evaluation of the potency of the anti-idiotypic antibody Ab2/3H6 mimicking gp41 as an HIV-1 vaccine in a rabbit prime/boost study.

Mader A, Kunert R.

PLoS One. 2012;7(6):e39063. doi: 10.1371/journal.pone.0039063. Epub 2012 Jun 15.

48.

Recombinant IgA production: single step affinity purification using camelid ligands and product characterization.

Reinhart D, Weik R, Kunert R.

J Immunol Methods. 2012 Apr 30;378(1-2):95-101.

PMID:
22570865
49.

A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells.

Jadhav V, Hackl M, Bort JA, Wieser M, Harreither E, Kunert R, Borth N, Grillari J.

Biotechnol Bioeng. 2012 Jun;109(6):1376-85. doi: 10.1002/bit.24490. Epub 2012 Mar 22.

PMID:
22407745
50.

Anti-idiotypic antibody Ab2/3H6 mimicking gp41: a potential HIV-1 vaccine?

Kunert R, Mader A.

BMC Proc. 2011 Nov 22;5 Suppl 8:P64. doi: 10.1186/1753-6561-5-S8-P64. eCollection 2011. No abstract available.

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