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Items: 1 to 50 of 137

1.

Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints.

Bezawork-Geleta A, Wen H, Dong L, Yan B, Vider J, Boukalova S, Krobova L, Vanova K, Zobalova R, Sobol M, Hozak P, Novais SM, Caisova V, Abaffy P, Naraine R, Pang Y, Zaw T, Zhang P, Sindelka R, Kubista M, Zuryn S, Molloy MP, Berridge MV, Pacak K, Rohlena J, Park S, Neuzil J.

Nat Commun. 2018 Jun 7;9(1):2221. doi: 10.1038/s41467-018-04603-z.

2.

Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan.

Sindelka R, Abaffy P, Qu Y, Tomankova S, Sidova M, Naraine R, Kolar M, Peuchen E, Sun L, Dovichi N, Kubista M.

Sci Rep. 2018 May 29;8(1):8315. doi: 10.1038/s41598-018-26592-1.

3.

Platforms for Single-Cell Collection and Analysis.

Valihrach L, Androvic P, Kubista M.

Int J Mol Sci. 2018 Mar 11;19(3). pii: E807. doi: 10.3390/ijms19030807. Review.

4.

Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes.

Virant-Klun I, Bauer C, Ståhlberg A, Kubista M, Skutella T.

Reprod Biomed Online. 2018 May;36(5):508-523. doi: 10.1016/j.rbmo.2018.01.011. Epub 2018 Feb 5.

PMID:
29503212
5.

A single-cell analysis reveals multiple roles of oligodendroglial lineage cells during post-ischemic regeneration.

Valny M, Honsa P, Waloschkova E, Matuskova H, Kriska J, Kirdajova D, Androvic P, Valihrach L, Kubista M, Anderova M.

Glia. 2018 May;66(5):1068-1081. doi: 10.1002/glia.23301. Epub 2018 Feb 2.

PMID:
29393544
6.

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification.

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M.

Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588.

7.

The secrets of the cell.

Kubista M, Dreyer-Lamm J, Ståhlberg A.

Mol Aspects Med. 2018 Feb;59:1-4. doi: 10.1016/j.mam.2017.08.004. Epub 2017 Aug 24. No abstract available.

PMID:
28826729
8.

Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer.

Bredemeier M, Edimiris P, Mach P, Kubista M, Sjöback R, Rohlova E, Kolostova K, Hauch S, Aktas B, Tewes M, Kimmig R, Kasimir-Bauer S.

Clin Chem. 2017 Oct;63(10):1585-1593. doi: 10.1373/clinchem.2016.269605. Epub 2017 Aug 3.

PMID:
28778937
9.

Technical aspects and recommendations for single-cell qPCR.

Ståhlberg A, Kubista M.

Mol Aspects Med. 2018 Feb;59:28-35. doi: 10.1016/j.mam.2017.07.004. Epub 2017 Jul 29. Review.

PMID:
28756182
10.

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR).

Forootan A, Sjöback R, Björkman J, Sjögreen B, Linz L, Kubista M.

Biomol Detect Quantif. 2017 Apr 29;12:1-6. doi: 10.1016/j.bdq.2017.04.001. eCollection 2017 Jun.

11.

Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early Development of Xenopus laevis.

Sindelka R, Sidova M, Abaffy P, Kubista M.

Results Probl Cell Differ. 2017;61:229-241. doi: 10.1007/978-3-319-53150-2_10. Review.

PMID:
28409307
12.

Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells.

Dong LF, Kovarova J, Bajzikova M, Bezawork-Geleta A, Svec D, Endaya B, Sachaphibulkij K, Coelho AR, Sebkova N, Ruzickova A, Tan AS, Kluckova K, Judasova K, Zamecnikova K, Rychtarcikova Z, Gopalan V, Andera L, Sobol M, Yan B, Pattnaik B, Bhatraju N, Truksa J, Stopka P, Hozak P, Lam AK, Sedlacek R, Oliveira PJ, Kubista M, Agrawal A, Dvorakova-Hortova K, Rohlena J, Berridge MV, Neuzil J.

Elife. 2017 Feb 15;6. pii: e22187. doi: 10.7554/eLife.22187.

13.

The correlation between expression profiles measured in single cells and in traditional bulk samples.

Dzamba D, Valihrach L, Kubista M, Anderova M.

Sci Rep. 2016 Nov 16;6:37022. doi: 10.1038/srep37022.

14.

Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single Circulating Tumor Cells.

Gorges TM, Kuske A, Röck K, Mauermann O, Müller V, Peine S, Verpoort K, Novosadova V, Kubista M, Riethdorf S, Pantel K.

Clin Chem. 2016 Nov;62(11):1504-1515. Epub 2016 Sep 14.

15.

The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data.

Korenkova V, Slyskova J, Novosadova V, Pizzamiglio S, Langerova L, Bjorkman J, Vycital O, Liska V, Levy M, Veskrna K, Vodicka P, Vodickova L, Kubista M, Verderio P.

Sci Rep. 2016 Jul 7;6:29023. doi: 10.1038/srep29023.

16.

Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog.

Honsa P, Valny M, Kriska J, Matuskova H, Harantova L, Kirdajova D, Valihrach L, Androvic P, Kubista M, Anderova M.

Glia. 2016 Sep;64(9):1518-31. doi: 10.1002/glia.23019. Epub 2016 Jun 24.

PMID:
27340757
17.

Gene expression profiling of circulating tumor cells and peripheral blood mononuclear cells from breast cancer patients.

Hensler M, Vančurová I, Becht E, Palata O, Strnad P, Tesařová P, Čabiňaková M, Švec D, Kubista M, Bartůňková J, Špíšek R, Sojka L.

Oncoimmunology. 2015 Dec 10;5(4):e1102827. eCollection 2016 Apr.

18.

Differential amplicons (ΔAmp)-a new molecular method to assess RNA integrity.

Björkman J, Švec D, Lott E, Kubista M, Sjöback R.

Biomol Detect Quantif. 2015 Sep 26;6:4-12. doi: 10.1016/j.bdq.2015.09.002. eCollection 2016 Jan.

19.

Effects of post-mortem and physical degradation on RNA integrity and quality.

Sidova M, Tomankova S, Abaffy P, Kubista M, Sindelka R.

Biomol Detect Quantif. 2015 Aug 21;5:3-9. doi: 10.1016/j.bdq.2015.08.002. eCollection 2015 Sep.

20.

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

Svec D, Tichopad A, Novosadova V, Pfaffl MW, Kubista M.

Biomol Detect Quantif. 2015 Mar 11;3:9-16. doi: 10.1016/j.bdq.2015.01.005. eCollection 2015 Mar.

21.

MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans.

Virant-Klun I, Ståhlberg A, Kubista M, Skutella T.

Stem Cells Int. 2016;2016:3984937. doi: 10.1155/2016/3984937. Epub 2015 Nov 9. Review.

22.

Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells.

Conrad S, Azizi H, Hatami M, Kubista M, Bonin M, Hennenlotter J, Sievert KD, Skutella T.

Stem Cells Int. 2016;2016:8582526. doi: 10.1155/2016/8582526. Epub 2015 Nov 8.

23.

Properties of targeted preamplification in DNA and cDNA quantification.

Andersson D, Akrap N, Svec D, Godfrey TE, Kubista M, Landberg G, Ståhlberg A.

Expert Rev Mol Diagn. 2015;15(8):1085-100. doi: 10.1586/14737159.2015.1057124. Epub 2015 Jul 1.

24.

Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division.

Sidova M, Sindelka R, Castoldi M, Benes V, Kubista M.

Sci Rep. 2015 Jun 10;5:11157. doi: 10.1038/srep11157.

25.

The influence of tissue procurement procedures on RNA integrity, gene expression, and morphology in porcine and human liver tissue.

Kap M, Sieuwerts AM, Kubista M, Oomen M, Arshad S, Riegman P.

Biopreserv Biobank. 2015 Jun;13(3):200-6. doi: 10.1089/bio.2014.0076.

PMID:
26035010
26.

Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of GFAP/EGFP Mice Following Ischemic Injury: Focus on NMDA Receptors.

Dzamba D, Honsa P, Valny M, Kriska J, Valihrach L, Novosadova V, Kubista M, Anderova M.

Cell Mol Neurobiol. 2015 Nov;35(8):1187-202. doi: 10.1007/s10571-015-0212-8. Epub 2015 May 21.

PMID:
25994914
27.

Multi-template polymerase chain reaction.

Kalle E, Kubista M, Rensing C.

Biomol Detect Quantif. 2014 Dec 4;2:11-29. eCollection 2014 Dec. Review.

28.

Altered astrocytic swelling in the cortex of α-syntrophin-negative GFAP/EGFP mice.

Anderova M, Benesova J, Mikesova M, Dzamba D, Honsa P, Kriska J, Butenko O, Novosadova V, Valihrach L, Kubista M, Dmytrenko L, Cicanic M, Vargova L.

PLoS One. 2014 Nov 26;9(11):e113444. doi: 10.1371/journal.pone.0113444. eCollection 2014. Erratum in: PLoS One. 2015;10(3):e0119744.

29.

SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.

Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, Günther K, Verderio P, Pizzamiglio S, Ciniselli CM, Zhang H, Korenková V, Rainen L, Bar T, Kubista M, Gelmini S.

PLoS One. 2014 Nov 10;9(11):e112293. doi: 10.1371/journal.pone.0112293. eCollection 2014.

30.

Biomarkers for monitoring pre-analytical quality variation of mRNA in blood samples.

Zhang H, Korenková V, Sjöback R, Švec D, Björkman J, Kruhøffer M, Verderio P, Pizzamiglio S, Ciniselli CM, Wyrich R, Oelmueller U, Kubista M, Lindahl T, Lönneborg A, Rian E.

PLoS One. 2014 Nov 4;9(11):e111644. doi: 10.1371/journal.pone.0111644. eCollection 2014.

31.

Increased expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in reactive astrocytes following ischemia.

Honsa P, Pivonkova H, Harantova L, Butenko O, Kriska J, Dzamba D, Rusnakova V, Valihrach L, Kubista M, Anderova M.

Glia. 2014 Dec;62(12):2004-21. doi: 10.1002/glia.22721. Epub 2014 Jul 14.

PMID:
25042871
32.

Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture.

Conrad S, Azizi H, Hatami M, Kubista M, Bonin M, Hennenlotter J, Renninger M, Skutella T.

Biomed Res Int. 2014;2014:138350. doi: 10.1155/2014/138350. Epub 2014 Mar 12.

33.

The workflow of single-cell expression profiling using quantitative real-time PCR.

Ståhlberg A, Kubista M.

Expert Rev Mol Diagn. 2014 Apr;14(3):323-31. doi: 10.1586/14737159.2014.901154. Review.

34.

Comparison of reverse transcription quantitative real-time PCR, flow cytometry, and immunohistochemistry for detection of monoclonality in lymphomas.

Ståhlberg A, Aman P, Strömbom L, Zoric N, Diez A, Nilsson O, Kubista M, Ridell B.

ISRN Oncol. 2014 Feb 4;2014:796210. doi: 10.1155/2014/796210. eCollection 2014.

35.

Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels in colorectal cancer patients.

Slyskova J, Cordero F, Pardini B, Korenkova V, Vymetalkova V, Bielik L, Vodickova L, Pitule P, Liska V, Matejka VM, Levy M, Buchler T, Kubista M, Naccarati A, Vodicka P.

Mol Carcinog. 2015 Sep;54(9):769-78. doi: 10.1002/mc.22141. Epub 2014 Mar 3.

36.

Direct cell lysis for single-cell gene expression profiling.

Svec D, Andersson D, Pekny M, Sjöback R, Kubista M, Ståhlberg A.

Front Oncol. 2013 Nov 7;3:274. doi: 10.3389/fonc.2013.00274. eCollection 2013.

37.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
38.

Heterogeneity of astrocytes: from development to injury - single cell gene expression.

Rusnakova V, Honsa P, Dzamba D, Ståhlberg A, Kubista M, Anderova M.

PLoS One. 2013 Aug 5;8(8):e69734. doi: 10.1371/journal.pone.0069734. Print 2013.

39.

Plasticity response in the contralesional hemisphere after subtle neurotrauma: gene expression profiling after partial deafferentation of the hippocampus.

Andersson D, Wilhelmsson U, Nilsson M, Kubista M, Ståhlberg A, Pekna M, Pekny M.

PLoS One. 2013 Jul 25;8(7):e70699. doi: 10.1371/journal.pone.0070699. Print 2013.

40.
41.

SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses.

Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, Hartmann CC, Verderio P, Pizzamiglio S, Ciniselli CM, Tichopad A, Kubista M, Gelmini S.

Clin Chim Acta. 2013 Sep 23;424:274-86. doi: 10.1016/j.cca.2013.05.012. Epub 2013 May 25.

PMID:
23714327
42.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

43.

Expression of pluripotency and oocyte-related genes in single putative stem cells from human adult ovarian surface epithelium cultured in vitro in the presence of follicular fluid.

Virant-Klun I, Skutella T, Kubista M, Vogler A, Sinkovec J, Meden-Vrtovec H.

Biomed Res Int. 2013;2013:861460. doi: 10.1155/2013/861460. Epub 2013 Feb 28.

44.

The added value of single-cell gene expression profiling.

Ståhlberg A, Rusnakova V, Kubista M.

Brief Funct Genomics. 2013 Mar;12(2):81-9. doi: 10.1093/bfgp/elt001. Epub 2013 Feb 7. Review.

PMID:
23393397
45.

SPIDIA-RNA: first external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.

Pazzagli M, Malentacchi F, Simi L, Orlando C, Wyrich R, Günther K, Hartmann CC, Verderio P, Pizzamiglio S, Ciniselli CM, Tichopad A, Kubista M, Gelmini S.

Methods. 2013 Jan;59(1):20-31. doi: 10.1016/j.ymeth.2012.10.007. Epub 2012 Oct 27.

PMID:
23110812
46.

RT-qPCR work-flow for single-cell data analysis.

Ståhlberg A, Rusnakova V, Forootan A, Anderova M, Kubista M.

Methods. 2013 Jan;59(1):80-8. doi: 10.1016/j.ymeth.2012.09.007. Epub 2012 Sep 25.

PMID:
23021995
47.

I see the light! And I see it again and again!

Kubista M.

Clin Chem. 2012 Nov;58(11):1505-6. doi: 10.1373/clinchem.2012.194126. Epub 2012 Sep 18. No abstract available.

48.

Potential stemness of frozen-thawed testicular biopsies without sperm in infertile men included into the in vitro fertilization programme.

Stimpfel M, Skutella T, Kubista M, Malicev E, Conrad S, Virant-Klun I.

J Biomed Biotechnol. 2012;2012:291038. doi: 10.1155/2012/291038. Epub 2012 Feb 8.

49.

Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis.

Günther K, Malentacchi F, Verderio P, Pizzamiglio S, Ciniselli CM, Tichopad A, Kubista M, Wyrich R, Pazzagli M, Gelmini S.

Clin Chim Acta. 2012 Apr 11;413(7-8):779-86. doi: 10.1016/j.cca.2012.01.015. Epub 2012 Jan 21.

PMID:
22285774
50.

Distinct expression/function of potassium and chloride channels contributes to the diverse volume regulation in cortical astrocytes of GFAP/EGFP mice.

Benesova J, Rusnakova V, Honsa P, Pivonkova H, Dzamba D, Kubista M, Anderova M.

PLoS One. 2012;7(1):e29725. doi: 10.1371/journal.pone.0029725. Epub 2012 Jan 11.

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