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Items: 1 to 50 of 145

1.

Multicenter Evaluation of Circulating Cell-Free DNA Extraction and Downstream Analyses for the Development of Standardized (Pre)analytical Work Flows.

Lampignano R, Neumann MHD, Weber S, Kloten V, Herdean A, Voss T, Groelz D, Babayan A, Tibbesma M, Schlumpberger M, Chemi F, Rothwell DG, Wikman H, Galizzi JP, Bergheim IR, Russnes H, Mussolin B, Bonin S, Voigt C, Musa H, Pinzani P, Lianidou E, Brady G, Speicher MR, Pantel K, Betsou F, Schuuring E, Kubista M, Ammerlaan W, Sprenger-Haussels M, Schlange T, Heitzer E; Innovative Medicines Initiative CANCER-ID Consortium.

Clin Chem. 2019 Oct 18. pii: clinchem.2019.306837. doi: 10.1373/clinchem.2019.306837. [Epub ahead of print]

PMID:
31628139
2.

Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients with Advanced Epithelial Ovarian Cancer.

Kumar J, Chudasama D, Roberts C, Kubista M, Sjöback R, Chatterjee J, Anikin V, Karteris E, Hall M.

Cells. 2019 Jul 17;8(7). pii: E732. doi: 10.3390/cells8070732.

3.

Multicenter Evaluation of Circulating Plasma MicroRNA Extraction Technologies for the Development of Clinically Feasible Reverse Transcription Quantitative PCR and Next-Generation Sequencing Analytical Work Flows.

Kloten V, Neumann MHD, Di Pasquale F, Sprenger-Haussels M, Shaffer JM, Schlumpberger M, Herdean A, Betsou F, Ammerlaan W, Af Hällström T, Serkkola E, Forsman T, Lianidou E, Sjöback R, Kubista M, Bender S, Lampignano R, Krahn T, Schlange T; CANCER-ID consortium.

Clin Chem. 2019 Sep;65(9):1132-1140. doi: 10.1373/clinchem.2019.303271. Epub 2019 Jun 24.

PMID:
31235535
4.

Pre-analytical processes in medical diagnostics: New regulatory requirements and standards.

Dagher G, Becker KF, Bonin S, Foy C, Gelmini S, Kubista M, Kungl P, Oelmueller U, Parkes H, Pinzani P, Riegman P, Schröder U, Stumptner C, Turano P, Sjöback R, Wutte A, Zatloukal K.

N Biotechnol. 2019 Sep 25;52:121-125. doi: 10.1016/j.nbt.2019.05.002. Epub 2019 May 15. Review.

PMID:
31102798
5.

Two-tailed RT-qPCR panel for quality control of circulating microRNA studies.

Androvic P, Romanyuk N, Urdzikova-Machova L, Rohlova E, Kubista M, Valihrach L.

Sci Rep. 2019 Mar 12;9(1):4255. doi: 10.1038/s41598-019-40513-w.

6.

Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem Cells Isolated from Adipose Tissue Derived from Horses with Equine Metabolic Syndrome.

Smieszek A, Kornicka K, Szłapka-Kosarzewska J, Androvic P, Valihrach L, Langerova L, Rohlova E, Kubista M, Marycz K.

Cells. 2019 Jan 22;8(2). pii: E80. doi: 10.3390/cells8020080.

7.

Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells.

Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal GJ, Magalhaes Novais S, Olsinova M, Krobova L, An YJ, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim TL, Hozak P, Sardao VA, Koopman WJH, Ricchetti M, Oliveira PJ, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J.

Cell Metab. 2019 Feb 5;29(2):399-416.e10. doi: 10.1016/j.cmet.2018.10.014. Epub 2018 Nov 15.

PMID:
30449682
8.

The Contribution of TRPV4 Channels to Astrocyte Volume Regulation and Brain Edema Formation.

Pivonkova H, Hermanova Z, Kirdajova D, Awadova T, Malinsky J, Valihrach L, Zucha D, Kubista M, Galisova A, Jirak D, Anderova M.

Neuroscience. 2018 Dec 1;394:127-143. doi: 10.1016/j.neuroscience.2018.10.028. Epub 2018 Oct 24.

PMID:
30367945
9.

Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints.

Bezawork-Geleta A, Wen H, Dong L, Yan B, Vider J, Boukalova S, Krobova L, Vanova K, Zobalova R, Sobol M, Hozak P, Novais SM, Caisova V, Abaffy P, Naraine R, Pang Y, Zaw T, Zhang P, Sindelka R, Kubista M, Zuryn S, Molloy MP, Berridge MV, Pacak K, Rohlena J, Park S, Neuzil J.

Nat Commun. 2018 Jun 7;9(1):2221. doi: 10.1038/s41467-018-04603-z.

10.

Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan.

Sindelka R, Abaffy P, Qu Y, Tomankova S, Sidova M, Naraine R, Kolar M, Peuchen E, Sun L, Dovichi N, Kubista M.

Sci Rep. 2018 May 29;8(1):8315. doi: 10.1038/s41598-018-26592-1.

11.

Platforms for Single-Cell Collection and Analysis.

Valihrach L, Androvic P, Kubista M.

Int J Mol Sci. 2018 Mar 11;19(3). pii: E807. doi: 10.3390/ijms19030807. Review.

12.

Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes.

Virant-Klun I, Bauer C, Ståhlberg A, Kubista M, Skutella T.

Reprod Biomed Online. 2018 May;36(5):508-523. doi: 10.1016/j.rbmo.2018.01.011. Epub 2018 Feb 5.

PMID:
29503212
13.

A single-cell analysis reveals multiple roles of oligodendroglial lineage cells during post-ischemic regeneration.

Valny M, Honsa P, Waloschkova E, Matuskova H, Kriska J, Kirdajova D, Androvic P, Valihrach L, Kubista M, Anderova M.

Glia. 2018 May;66(5):1068-1081. doi: 10.1002/glia.23301. Epub 2018 Feb 2.

PMID:
29393544
14.

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification.

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M.

Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588.

15.

The secrets of the cell.

Kubista M, Dreyer-Lamm J, Ståhlberg A.

Mol Aspects Med. 2018 Feb;59:1-4. doi: 10.1016/j.mam.2017.08.004. Epub 2017 Aug 24. No abstract available.

PMID:
28826729
16.

Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer.

Bredemeier M, Edimiris P, Mach P, Kubista M, Sjöback R, Rohlova E, Kolostova K, Hauch S, Aktas B, Tewes M, Kimmig R, Kasimir-Bauer S.

Clin Chem. 2017 Oct;63(10):1585-1593. doi: 10.1373/clinchem.2016.269605. Epub 2017 Aug 3.

17.

Technical aspects and recommendations for single-cell qPCR.

Ståhlberg A, Kubista M.

Mol Aspects Med. 2018 Feb;59:28-35. doi: 10.1016/j.mam.2017.07.004. Epub 2017 Jul 29. Review.

PMID:
28756182
18.

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR).

Forootan A, Sjöback R, Björkman J, Sjögreen B, Linz L, Kubista M.

Biomol Detect Quantif. 2017 Apr 29;12:1-6. doi: 10.1016/j.bdq.2017.04.001. eCollection 2017 Jun.

19.

Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early Development of Xenopus laevis.

Sindelka R, Sidova M, Abaffy P, Kubista M.

Results Probl Cell Differ. 2017;61:229-241. doi: 10.1007/978-3-319-53150-2_10. Review.

PMID:
28409307
20.

Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells.

Dong LF, Kovarova J, Bajzikova M, Bezawork-Geleta A, Svec D, Endaya B, Sachaphibulkij K, Coelho AR, Sebkova N, Ruzickova A, Tan AS, Kluckova K, Judasova K, Zamecnikova K, Rychtarcikova Z, Gopalan V, Andera L, Sobol M, Yan B, Pattnaik B, Bhatraju N, Truksa J, Stopka P, Hozak P, Lam AK, Sedlacek R, Oliveira PJ, Kubista M, Agrawal A, Dvorakova-Hortova K, Rohlena J, Berridge MV, Neuzil J.

Elife. 2017 Feb 15;6. pii: e22187. doi: 10.7554/eLife.22187.

21.

The correlation between expression profiles measured in single cells and in traditional bulk samples.

Dzamba D, Valihrach L, Kubista M, Anderova M.

Sci Rep. 2016 Nov 16;6:37022. doi: 10.1038/srep37022.

22.

Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single Circulating Tumor Cells.

Gorges TM, Kuske A, Röck K, Mauermann O, Müller V, Peine S, Verpoort K, Novosadova V, Kubista M, Riethdorf S, Pantel K.

Clin Chem. 2016 Nov;62(11):1504-1515. Epub 2016 Sep 14.

23.

The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data.

Korenkova V, Slyskova J, Novosadova V, Pizzamiglio S, Langerova L, Bjorkman J, Vycital O, Liska V, Levy M, Veskrna K, Vodicka P, Vodickova L, Kubista M, Verderio P.

Sci Rep. 2016 Jul 7;6:29023. doi: 10.1038/srep29023.

24.

Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog.

Honsa P, Valny M, Kriska J, Matuskova H, Harantova L, Kirdajova D, Valihrach L, Androvic P, Kubista M, Anderova M.

Glia. 2016 Sep;64(9):1518-31. doi: 10.1002/glia.23019. Epub 2016 Jun 24.

PMID:
27340757
25.

Gene expression profiling of circulating tumor cells and peripheral blood mononuclear cells from breast cancer patients.

Hensler M, Vančurová I, Becht E, Palata O, Strnad P, Tesařová P, Čabiňaková M, Švec D, Kubista M, Bartůňková J, Špíšek R, Sojka L.

Oncoimmunology. 2015 Dec 10;5(4):e1102827. eCollection 2016 Apr.

26.

Differential amplicons (ΔAmp)-a new molecular method to assess RNA integrity.

Björkman J, Švec D, Lott E, Kubista M, Sjöback R.

Biomol Detect Quantif. 2015 Sep 26;6:4-12. doi: 10.1016/j.bdq.2015.09.002. eCollection 2016 Jan.

27.

Effects of post-mortem and physical degradation on RNA integrity and quality.

Sidova M, Tomankova S, Abaffy P, Kubista M, Sindelka R.

Biomol Detect Quantif. 2015 Aug 21;5:3-9. doi: 10.1016/j.bdq.2015.08.002. eCollection 2015 Sep.

28.

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

Svec D, Tichopad A, Novosadova V, Pfaffl MW, Kubista M.

Biomol Detect Quantif. 2015 Mar 11;3:9-16. doi: 10.1016/j.bdq.2015.01.005. eCollection 2015 Mar.

29.

MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans.

Virant-Klun I, Ståhlberg A, Kubista M, Skutella T.

Stem Cells Int. 2016;2016:3984937. doi: 10.1155/2016/3984937. Epub 2015 Nov 9. Review.

30.

Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells.

Conrad S, Azizi H, Hatami M, Kubista M, Bonin M, Hennenlotter J, Sievert KD, Skutella T.

Stem Cells Int. 2016;2016:8582526. doi: 10.1155/2016/8582526. Epub 2015 Nov 8.

31.

Properties of targeted preamplification in DNA and cDNA quantification.

Andersson D, Akrap N, Svec D, Godfrey TE, Kubista M, Landberg G, Ståhlberg A.

Expert Rev Mol Diagn. 2015;15(8):1085-100. doi: 10.1586/14737159.2015.1057124. Epub 2015 Jul 1.

32.

Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division.

Sidova M, Sindelka R, Castoldi M, Benes V, Kubista M.

Sci Rep. 2015 Jun 10;5:11157. doi: 10.1038/srep11157.

33.

The influence of tissue procurement procedures on RNA integrity, gene expression, and morphology in porcine and human liver tissue.

Kap M, Sieuwerts AM, Kubista M, Oomen M, Arshad S, Riegman P.

Biopreserv Biobank. 2015 Jun;13(3):200-6. doi: 10.1089/bio.2014.0076.

PMID:
26035010
34.

Quantitative Analysis of Glutamate Receptors in Glial Cells from the Cortex of GFAP/EGFP Mice Following Ischemic Injury: Focus on NMDA Receptors.

Dzamba D, Honsa P, Valny M, Kriska J, Valihrach L, Novosadova V, Kubista M, Anderova M.

Cell Mol Neurobiol. 2015 Nov;35(8):1187-202. doi: 10.1007/s10571-015-0212-8. Epub 2015 May 21.

PMID:
25994914
35.

Multi-template polymerase chain reaction.

Kalle E, Kubista M, Rensing C.

Biomol Detect Quantif. 2014 Dec 4;2:11-29. eCollection 2014 Dec. Review.

36.

Altered astrocytic swelling in the cortex of α-syntrophin-negative GFAP/EGFP mice.

Anderova M, Benesova J, Mikesova M, Dzamba D, Honsa P, Kriska J, Butenko O, Novosadova V, Valihrach L, Kubista M, Dmytrenko L, Cicanic M, Vargova L.

PLoS One. 2014 Nov 26;9(11):e113444. doi: 10.1371/journal.pone.0113444. eCollection 2014. Erratum in: PLoS One. 2015;10(3):e0119744.

37.

SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.

Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, Günther K, Verderio P, Pizzamiglio S, Ciniselli CM, Zhang H, Korenková V, Rainen L, Bar T, Kubista M, Gelmini S.

PLoS One. 2014 Nov 10;9(11):e112293. doi: 10.1371/journal.pone.0112293. eCollection 2014.

38.

Biomarkers for monitoring pre-analytical quality variation of mRNA in blood samples.

Zhang H, Korenková V, Sjöback R, Švec D, Björkman J, Kruhøffer M, Verderio P, Pizzamiglio S, Ciniselli CM, Wyrich R, Oelmueller U, Kubista M, Lindahl T, Lönneborg A, Rian E.

PLoS One. 2014 Nov 4;9(11):e111644. doi: 10.1371/journal.pone.0111644. eCollection 2014.

39.

Increased expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in reactive astrocytes following ischemia.

Honsa P, Pivonkova H, Harantova L, Butenko O, Kriska J, Dzamba D, Rusnakova V, Valihrach L, Kubista M, Anderova M.

Glia. 2014 Dec;62(12):2004-21. doi: 10.1002/glia.22721. Epub 2014 Jul 14.

PMID:
25042871
40.

Differential gene expression profiling of enriched human spermatogonia after short- and long-term culture.

Conrad S, Azizi H, Hatami M, Kubista M, Bonin M, Hennenlotter J, Renninger M, Skutella T.

Biomed Res Int. 2014;2014:138350. doi: 10.1155/2014/138350. Epub 2014 Mar 12.

41.

The workflow of single-cell expression profiling using quantitative real-time PCR.

Ståhlberg A, Kubista M.

Expert Rev Mol Diagn. 2014 Apr;14(3):323-31. doi: 10.1586/14737159.2014.901154. Review.

42.

Comparison of reverse transcription quantitative real-time PCR, flow cytometry, and immunohistochemistry for detection of monoclonality in lymphomas.

Ståhlberg A, Aman P, Strömbom L, Zoric N, Diez A, Nilsson O, Kubista M, Ridell B.

ISRN Oncol. 2014 Feb 4;2014:796210. doi: 10.1155/2014/796210. eCollection 2014.

43.

Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels in colorectal cancer patients.

Slyskova J, Cordero F, Pardini B, Korenkova V, Vymetalkova V, Bielik L, Vodickova L, Pitule P, Liska V, Matejka VM, Levy M, Buchler T, Kubista M, Naccarati A, Vodicka P.

Mol Carcinog. 2015 Sep;54(9):769-78. doi: 10.1002/mc.22141. Epub 2014 Mar 3.

44.

Direct cell lysis for single-cell gene expression profiling.

Svec D, Andersson D, Pekny M, Sjöback R, Kubista M, Ståhlberg A.

Front Oncol. 2013 Nov 7;3:274. doi: 10.3389/fonc.2013.00274. eCollection 2013.

45.

The need for transparency and good practices in the qPCR literature.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J.

Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

PMID:
24173381
46.

Heterogeneity of astrocytes: from development to injury - single cell gene expression.

Rusnakova V, Honsa P, Dzamba D, Ståhlberg A, Kubista M, Anderova M.

PLoS One. 2013 Aug 5;8(8):e69734. doi: 10.1371/journal.pone.0069734. Print 2013.

47.

Plasticity response in the contralesional hemisphere after subtle neurotrauma: gene expression profiling after partial deafferentation of the hippocampus.

Andersson D, Wilhelmsson U, Nilsson M, Kubista M, Ståhlberg A, Pekna M, Pekny M.

PLoS One. 2013 Jul 25;8(7):e70699. doi: 10.1371/journal.pone.0070699. Print 2013.

48.
49.

SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses.

Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, Hartmann CC, Verderio P, Pizzamiglio S, Ciniselli CM, Tichopad A, Kubista M, Gelmini S.

Clin Chim Acta. 2013 Sep 23;424:274-86. doi: 10.1016/j.cca.2013.05.012. Epub 2013 May 25.

PMID:
23714327
50.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

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