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Mol Cell. 2019 Mar 7;73(5):1066-1074.e3. doi: 10.1016/j.molcel.2018.12.005. Epub 2019 Jan 17.

Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation.

Author information

1
Universidad de Buenos Aires (UBA), Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular and CONICET-UBA, Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), (C1428EHA), Buenos Aires, Argentina.
2
Department of Protein Biosynthesis, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
3
Division of Plant Sciences, University of Dundee at The James Hutton Institute, Invergowrie, Dundee, Scotland.
4
Universidad de Buenos Aires (UBA), Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular and CONICET-UBA, Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), (C1428EHA), Buenos Aires, Argentina. Electronic address: ark@fbmc.fcen.uba.ar.

Abstract

Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts. Here, we show that light promotes RNA polymerase II (Pol II) elongation in the affected genes, whereas in darkness, elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing, as revealed by different drug treatments and genetic evidence. The light control of splicing and elongation is abolished in an Arabidopsis mutant defective in the transcription factor IIS (TFIIS). We report that the chloroplast control of nuclear alternative splicing in plants responds to the kinetic coupling mechanism found in mammalian cells, providing unique evidence that coupling is important for a whole organism to respond to environmental cues.

KEYWORDS:

alternative splicing; light control in plants; transcription elongation

PMID:
30661982
DOI:
10.1016/j.molcel.2018.12.005
[Indexed for MEDLINE]

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