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Anticancer Res. 2016 Jan;36(1):221-9.

Growth Inhibition of Osteosarcoma Cell Lines in 3D Cultures: Role of Nitrosative and Oxidative Stress.

Author information

1
Department of Medical Chemistry, Medical University of Gdansk, Gdansk, Poland m.gorska@gumed.edu.pl karolinasiwicka@gumed.edu.pl mzmijewski@gumed.edu.pl.
2
Department of Histology, Medical University of Gdansk, Gdansk, Poland.
3
Department of Medical Chemistry, Medical University of Gdansk, Gdansk, Poland.
4
Department of Histology, Medical University of Gdansk, Gdansk, Poland m.gorska@gumed.edu.pl karolinasiwicka@gumed.edu.pl mzmijewski@gumed.edu.pl.
5
Department of Medical Chemistry, Medical University of Gdansk, Gdansk, Poland Department of Kinesiology, Gdansk University of Physical Education and Sport, Gdansk, Poland m.gorska@gumed.edu.pl karolinasiwicka@gumed.edu.pl mzmijewski@gumed.edu.pl.

Abstract

BACKGROUND:

3D cell cultures have revolutionized the understanding of cell behavior, allowing culture of cells with the possibility of resembling in vivo intercellular signaling and cell-extracellular matrix interaction.

AIM:

The effect of limited oxygen penetration into 3D culture of highly metastatic osteosarcoma 143B cells in terms of expression of nitro-oxidative stress markers was investigated and compared to standard 2D cell culture.

MATERIALS AND METHODS:

Human osteosarcoma (143B cell line) cells were cultured as monolayers, in collagen and Matrigel. Cell viability, gene expression of nitro-oxidative stress markers, and vascular endothelial growth factor were determined using Trypan blue assay, quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.

RESULTS:

Three-dimensional environments modify nitro-oxidative stress and influence gene expression and cell proliferation of OS 143B cells.

CONCLUSION:

Commercial cell lines might not constitute a good model of 3D cultures for bone tissue engineering, as they are highly sensitive to hypoxia, and hypoxic conditions can induce oxidation of the cellular environment.

KEYWORDS:

ECM; Extracellular matrix; OS 143B cell line; monolayer/spatial (2D/3D) cell culture; nitro-oxidative stress

PMID:
26722047
[Indexed for MEDLINE]

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