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Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15716-21. doi: 10.1073/pnas.1305420110. Epub 2013 Sep 9.

Dissection of Cdk1-cyclin complexes in vivo.

Author information

1
Département de Biochimie and Centre Robert-Cedergren, Bio-Informatique et Génomique, Université de Montréal, Montréal, QC, Canada H3C 3J7.

Abstract

Cyclin-dependent kinases (Cdks) are regulatory enzymes with temporal and spatial selectivity for their protein substrates that are governed by cell cycle-regulated cyclin subunits. Specific cyclin-Cdk complexes bind to and phosphorylate target proteins, coupling their activity to cell cycle states. The identification of specific cyclin-Cdk substrates is challenging and so far, has largely been achieved through indirect correlation or use of in vitro techniques. Here, we use a protein-fragment complementation assay based on the optimized yeast cytosine deaminase to systematically identify candidate substrates of budding yeast Saccharomyces cerevisiae Cdk1 and show dependency on one or more regulatory cyclins. We identified known and candidate cyclin dependencies for many predicted protein kinase Cdk1 targets and showed elusory Clb3-Cdk1-specific phosphorylation of γ-tubulin, thus establishing the timing of this event in controlling assembly of the mitotic spindle. Our strategy can be generally applied to identify substrates and accessory subunits of multisubunit protein complexes.

KEYWORDS:

cyclin specificity; in vivo enzyme complexes screen

PMID:
24019491
PMCID:
PMC3785786
DOI:
10.1073/pnas.1305420110
[Indexed for MEDLINE]
Free PMC Article

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