Format

Send to

Choose Destination
eNeuro. 2017 Mar 9;4(1). pii: ENEURO.0255-16.2017. doi: 10.1523/ENEURO.0255-16.2017. eCollection 2017 Jan-Feb.

NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons.

Author information

1
Molecular Biology and Genetics Department, Koç University, Istanbul 34450, Turkey.
2
Physics Department, Koç University, Istanbul 34450, Turkey.

Abstract

Calcium signaling controls many key processes in neurons, including gene expression, axon guidance, and synaptic plasticity. In contrast to calcium influx through voltage- or neurotransmitter-gated channels, regulatory pathways that control store-operated calcium entry (SOCE) in neurons are poorly understood. Here, we report a transcriptional control of Stim1 (stromal interaction molecule 1) gene, which is a major sensor of endoplasmic reticulum (ER) calcium levels and a regulator of SOCE. By using a genome-wide chromatin immunoprecipitation and sequencing approach in mice, we find that NEUROD2, a neurogenic transcription factor, binds to an intronic element within the Stim1 gene. We show that NEUROD2 limits Stim1 expression in cortical neurons and consequently fine-tunes the SOCE response upon depletion of ER calcium. Our findings reveal a novel mechanism that regulates neuronal calcium homeostasis during cortical development.

KEYWORDS:

Neurod2; calcium; genomics; store-operated calcium entry; transcription factor

PMID:
28303257
PMCID:
PMC5343279
DOI:
10.1523/ENEURO.0255-16.2017
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center