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Items: 1 to 50 of 108

1.

Carcinoembryonic antigen carrying SLeX as a new biomarker of more aggressive gastric carcinomas.

Gomes C, Almeida A, Barreira A, Calheiros J, Pinto F, Abrantes R, Costa A, Polonia A, Campos D, Osório H, Sousa H, Pinto-de-Sousa J, Kolarich D, Reis CA.

Theranostics. 2019 Oct 11;9(24):7431-7446. doi: 10.7150/thno.33858. eCollection 2019.

2.

Glycan size and attachment site location affect electron transfer dissociation (ETD) fragmentation and automated glycopeptide identification.

Alagesan K, Hinneburg H, Seeberger PH, Silva DV, Kolarich D.

Glycoconj J. 2019 Dec;36(6):487-493. doi: 10.1007/s10719-019-09888-w. Epub 2019 Oct 21.

PMID:
31637569
3.

NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.

De Leoz MLA, Duewer DL, Fung A, Liu L, Yau HK, Potter O, Staples GO, Furuki K, Frenkel R, Hu Y, Sosic Z, Zhang P, Altmann F, Gru Nwald-Grube C, Shao C, Zaia J, Evers W, Pengelley S, Suckau D, Wiechmann A, Resemann A, Jabs W, Beck A, Froehlich JW, Huang C, Li Y, Liu Y, Sun S, Wang Y, Seo Y, An HJ, Reichardt NC, Ruiz JE, Archer-Hartmann S, Azadi P, Bell L, Lakos Z, An Y, Cipollo JF, Pucic-Bakovic M, Štambuk J, Lauc G, Li X, Wang PG, Bock A, Hennig R, Rapp E, Creskey M, Cyr TD, Nakano M, Sugiyama T, Leung PA, Link-Lenczowski P, Jaworek J, Yang S, Zhang H, Kelly T, Klapoetke S, Cao R, Kim JY, Lee HK, Lee JY, Yoo JS, Kim SR, Suh SK, de Haan N, Falck D, Lageveen-Kammeijer GSM, Wuhrer M, Emery RJ, Kozak RP, Liew LP, Royle L, Urbanowicz PA, Packer NH, Song X, Everest-Dass A, Lattová E, Cajic S, Alagesan K, Kolarich D, Kasali T, Lindo V, Chen Y, Goswami K, Gau B, Amunugama R, Jones R, Stroop CJM, Kato K, Yagi H, Kondo S, Yuen CT, Harazono A, Shi X, Magnelli PE, Kasper BT, Mahal L, Harvey DJ, O'Flaherty R, Rudd PM, Saldova R, Hecht ES, Muddiman DC, Kang J, Bhoskar P, Menard D, Saati A, Merle C, Mast S, Tep S, Truong J, Nishikaze T, Sekiya S, Shafer A, Funaoka S, Toyoda M, de Vreugd P, Caron C, Pradhan P, Tan NC, Mechref Y, Patil S, Rohrer JS, Chakrabarti R, Dadke D, Lahori M, Zou C, Cairo C, Reiz B, Whittal RM, Lebrilla CB, Wu L, Guttman A, Szigeti M, Kremkow BG, Lee KH, Sihlbom C, Adamczyk B, Jin C, Karlsson NG, Örnros J, Larson G, Nilsson J, Meyer B, Wiegandt A, Komatsu E, Perreault H, Bodnar ED, Said N, Francois YN, Leize-Wagner E, Maier S, Zeck A, Heck AJR, Yang Y, Haselberg R, Yu YQ, Alley W, Leone JW, Yuan H, Stein SE.

Mol Cell Proteomics. 2020 Jan;19(1):11-30. doi: 10.1074/mcp.RA119.001677. Epub 2019 Oct 7.

4.

Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers.

Chatterjee S, Lee LY, Kawahara R, Abrahams JL, Adamczyk B, Anugraham M, Ashwood C, Sumer-Bayraktar Z, Briggs MT, Chik JHL, Everest-Dass A, Förster S, Hinneburg H, Leite KRM, Loke I, Möginger U, Moh ESX, Nakano M, Recuero S, Sethi MK, Srougi M, Stavenhagen K, Venkatakrishnan V, Wongtrakul-Kish K, Diestel S, Hoffmann P, Karlsson NG, Kolarich D, Molloy MP, Muders MH, Oehler MK, Packer NH, Palmisano G, Thaysen-Andersen M.

Proteomics. 2019 Nov;19(21-22):e1900010. doi: 10.1002/pmic.201900010. Epub 2019 Oct 16.

PMID:
31419058
5.

Towards a standardized bioinformatics infrastructure for N- and O-glycomics.

Rojas-Macias MA, Mariethoz J, Andersson P, Jin C, Venkatakrishnan V, Aoki NP, Shinmachi D, Ashwood C, Madunic K, Zhang T, Miller RL, Horlacher O, Struwe WB, Watanabe Y, Okuda S, Levander F, Kolarich D, Rudd PM, Wuhrer M, Kettner C, Packer NH, Aoki-Kinoshita KF, Lisacek F, Karlsson NG.

Nat Commun. 2019 Jul 22;10(1):3275. doi: 10.1038/s41467-019-11131-x. Review.

6.

Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder.

Alagesan K, Kolarich D.

MethodsX. 2019 Apr 9;6:773-778. doi: 10.1016/j.mex.2019.04.007. eCollection 2019.

7.

The minimum information required for a glycomics experiment (MIRAGE) project: LC guidelines.

Campbell MP, Abrahams JL, Rapp E, Struwe WB, Costello CE, Novotny M, Ranzinger R, York WS, Kolarich D, Rudd PM, Kettner C.

Glycobiology. 2019 May 1;29(5):349-354. doi: 10.1093/glycob/cwz009.

PMID:
30778580
8.

GlyConnect: Glycoproteomics Goes Visual, Interactive, and Analytical.

Alocci D, Mariethoz J, Gastaldello A, Gasteiger E, Karlsson NG, Kolarich D, Packer NH, Lisacek F.

J Proteome Res. 2019 Feb 1;18(2):664-677. doi: 10.1021/acs.jproteome.8b00766. Epub 2019 Jan 15.

PMID:
30574787
9.

Isomeric Separation and Characterisation of Glycoconjugates.

Alagesan K, Everest-Dass A, Kolarich D.

Adv Exp Med Biol. 2018;1104:77-99. doi: 10.1007/978-981-13-2158-0_5. Review.

PMID:
30484245
10.

A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.

Tomek MB, Maresch D, Windwarder M, Friedrich V, Janesch B, Fuchs K, Neumann L, Nimeth I, Zwickl NF, Dohm JC, Everest-Dass A, Kolarich D, Himmelbauer H, Altmann F, Schäffer C.

Front Microbiol. 2018 Aug 28;9:2008. doi: 10.3389/fmicb.2018.02008. eCollection 2018.

11.

Human Mast Cell Tryptase Is a Potential Treatment for Snakebite Envenoming Across Multiple Snake Species.

Anderson E, Stavenhagen K, Kolarich D, Sommerhoff CP, Maurer M, Metz M.

Front Immunol. 2018 Jul 9;9:1532. doi: 10.3389/fimmu.2018.01532. eCollection 2018.

12.

Alterations of the Human Skin N- and O-Glycome in Basal Cell Carcinoma and Squamous Cell Carcinoma.

Möginger U, Grunewald S, Hennig R, Kuo CW, Schirmeister F, Voth H, Rapp E, Khoo KH, Seeberger PH, Simon JC, Kolarich D.

Front Oncol. 2018 Mar 21;8:70. doi: 10.3389/fonc.2018.00070. eCollection 2018.

13.

Flagellin Glycoproteomics of the Periodontitis Associated Pathogen Selenomonas sputigena Reveals Previously Not Described O-glycans and Rhamnose Fragment Rearrangement Occurring on the Glycopeptides.

Rath CB, Schirmeister F, Figl R, Seeberger PH, Schäffer C, Kolarich D.

Mol Cell Proteomics. 2018 Apr;17(4):721-736. doi: 10.1074/mcp.RA117.000394. Epub 2018 Jan 16.

14.

Exploring the Glycans of Euglena gracilis.

O'Neill EC, Kuhaudomlarp S, Rejzek M, Fangel JU, Alagesan K, Kolarich D, Willats WGT, Field RA.

Biology (Basel). 2017 Dec 15;6(4). pii: E45. doi: 10.3390/biology6040045.

15.

PepSweetener: A Web-Based Tool to Support Manual Annotation of Intact Glycopeptide MS Spectra.

Domagalski MJ, Alocci D, Almeida A, Kolarich D, Lisacek F.

Proteomics Clin Appl. 2018 Sep;12(5):e1700069. doi: 10.1002/prca.201700069. Epub 2017 Oct 30.

PMID:
28975713
16.

Unlocking Cancer Glycomes from Histopathological Formalin-fixed and Paraffin-embedded (FFPE) Tissue Microdissections.

Hinneburg H, Korać P, Schirmeister F, Gasparov S, Seeberger PH, Zoldoš V, Kolarich D.

Mol Cell Proteomics. 2017 Apr;16(4):524-536. doi: 10.1074/mcp.M116.062414. Epub 2017 Jan 25.

17.

The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data.

Liu Y, McBride R, Stoll M, Palma AS, Silva L, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A, Haslam SM, Karlsson NG, Khoo KH, Kolarich D, Novotny MV, Packer NH, Ranzinger R, Rapp E, Rudd PM, Struwe WB, Tiemeyer M, Wells L, York WS, Zaia J, Kettner C, Paulson JC, Feizi T, Smith DF.

Glycobiology. 2017 Apr;27(4):280-284. Epub 2016 Nov 22.

18.

It is all about the solvent: on the importance of the mobile phase for ZIC-HILIC glycopeptide enrichment.

Alagesan K, Khilji SK, Kolarich D.

Anal Bioanal Chem. 2017 Jan;409(2):529-538. doi: 10.1007/s00216-016-0051-6. Epub 2016 Dec 1.

19.

Detection and first characterization of an uncommon haptoglobin in porcine saliva of pigs with rectal prolapse by using boronic acid sample enrichment.

Gutiérrez AM, Miller I, Kolarich D, Hummel K, Nöbauer K, Razzazi-Fazeli E.

Animal. 2017 May;11(5):845-853. doi: 10.1017/S1751731116002159. Epub 2016 Nov 10.

PMID:
27829481
20.

N- and O-Glycomics from Minor Amounts of Formalin-Fixed, Paraffin-Embedded Tissue Samples.

Hinneburg H, Schirmeister F, Korać P, Kolarich D.

Methods Mol Biol. 2017;1503:131-145.

PMID:
27743364
21.

Site-Specific N- and O-Glycopeptide Analysis Using an Integrated C18-PGC-LC-ESI-QTOF-MS/MS Approach.

Stavenhagen K, Hinneburg H, Kolarich D, Wuhrer M.

Methods Mol Biol. 2017;1503:109-119.

PMID:
27743362
22.

The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets.

Struwe WB, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A, Ten Feizi, Haslam SM, Karlsson NG, Khoo KH, Kolarich D, Liu Y, McBride R, Novotny MV, Packer NH, Paulson JC, Rapp E, Ranzinger R, Rudd PM, Smith DF, Tiemeyer M, Wells L, York WS, Zaia J, Kettner C.

Glycobiology. 2016 Sep;26(9):907-910. Epub 2016 Sep 21.

23.

Blood group antigen expression is involved in C. albicans interaction with buccal epithelial cells.

Everest-Dass AV, Kolarich D, Pascovici D, Packer NH.

Glycoconj J. 2017 Feb;34(1):31-50. doi: 10.1007/s10719-016-9726-7. Epub 2016 Sep 17.

PMID:
27639389
24.

Glycomics & Glycoproteomics in Glycoconjugate journal.

Kolarich D, Wuhrer M.

Glycoconj J. 2016 Jun;33(3):259-60. doi: 10.1007/s10719-016-9674-2. No abstract available.

PMID:
27178342
25.

Emerging roles of protein mannosylation in inflammation and infection.

Loke I, Kolarich D, Packer NH, Thaysen-Andersen M.

Mol Aspects Med. 2016 Oct;51:31-55. doi: 10.1016/j.mam.2016.04.004. Epub 2016 Apr 13. Review.

PMID:
27086127
26.

Glycomic and sialoproteomic data of gastric carcinoma cells overexpressing ST3GAL4.

Mereiter S, Magalhães A, Adamczyk B, Jin C, Almeida A, Drici L, Ibáñez-Vea M, Larsen MR, Kolarich D, Karlsson NG, Reis CA.

Data Brief. 2016 Mar 14;7:814-33. doi: 10.1016/j.dib.2016.03.022. eCollection 2016 Jun.

27.

The promise of protein glycosylation for personalised medicine.

Almeida A, Kolarich D.

Biochim Biophys Acta. 2016 Aug;1860(8):1583-95. doi: 10.1016/j.bbagen.2016.03.012. Epub 2016 Mar 15. Review.

PMID:
26987810
28.

Distinguishing N-acetylneuraminic acid linkage isomers on glycopeptides by ion mobility-mass spectrometry.

Hinneburg H, Hofmann J, Struwe WB, Thader A, Altmann F, Varón Silva D, Seeberger PH, Pagel K, Kolarich D.

Chem Commun (Camb). 2016 Mar 21;52(23):4381-4. doi: 10.1039/c6cc01114d.

PMID:
26926577
29.

Cross Reactive Material 197 glycoconjugate vaccines contain privileged conjugation sites.

Möginger U, Resemann A, Martin CE, Parameswarappa S, Govindan S, Wamhoff EC, Broecker F, Suckau D, Pereira CL, Anish C, Seeberger PH, Kolarich D.

Sci Rep. 2016 Feb 4;6:20488. doi: 10.1038/srep20488.

30.

The Art of Destruction: Optimizing Collision Energies in Quadrupole-Time of Flight (Q-TOF) Instruments for Glycopeptide-Based Glycoproteomics.

Hinneburg H, Stavenhagen K, Schweiger-Hufnagel U, Pengelley S, Jabs W, Seeberger PH, Silva DV, Wuhrer M, Kolarich D.

J Am Soc Mass Spectrom. 2016 Mar;27(3):507-19. doi: 10.1007/s13361-015-1308-6. Epub 2016 Jan 4.

31.

Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer.

Mereiter S, Magalhães A, Adamczyk B, Jin C, Almeida A, Drici L, Ibáñez-Vea M, Gomes C, Ferreira JA, Afonso LP, Santos LL, Larsen MR, Kolarich D, Karlsson NG, Reis CA.

Biochim Biophys Acta. 2016 Aug;1860(8):1795-808. doi: 10.1016/j.bbagen.2015.12.016. Epub 2015 Dec 22.

PMID:
26721331
32.

An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo.

Lohse S, Meyer S, Meulenbroek LA, Jansen JH, Nederend M, Kretschmer A, Klausz K, Möginger U, Derer S, Rösner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen JH, Valerius T.

Cancer Res. 2016 Jan 15;76(2):403-17. doi: 10.1158/0008-5472.CAN-15-1232. Epub 2015 Dec 3.

33.

Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines : HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.

Ito H, Kaji H, Togayachi A, Azadi P, Ishihara M, Geyer R, Galuska C, Geyer H, Kakehi K, Kinoshita M, Karlsson NG, Jin C, Kato K, Yagi H, Kondo S, Kawasaki N, Hashii N, Kolarich D, Stavenhagen K, Packer NH, Thaysen-Andersen M, Nakano M, Taniguchi N, Kurimoto A, Wada Y, Tajiri M, Yang P, Cao W, Li H, Rudd PM, Narimatsu H.

Glycoconj J. 2016 Jun;33(3):405-415. doi: 10.1007/s10719-015-9625-3. Epub 2015 Oct 28.

34.

Flagellin glycosylation in Paenibacillus alvei CCM 2051T.

Janesch B, Schirmeister F, Maresch D, Altmann F, Messner P, Kolarich D, Schäffer C.

Glycobiology. 2016 Jan;26(1):74-87. doi: 10.1093/glycob/cwv087. Epub 2015 Sep 24.

35.

Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer.

Carvalho S, Catarino TA, Dias AM, Kato M, Almeida A, Hessling B, Figueiredo J, Gärtner F, Sanches JM, Ruppert T, Miyoshi E, Pierce M, Carneiro F, Kolarich D, Seruca R, Yamaguchi Y, Taniguchi N, Reis CA, Pinho SS.

Oncogene. 2016 Mar 31;35(13):1619-31. doi: 10.1038/onc.2015.225. Epub 2015 Jul 20.

36.

Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.

Kolarich D, Windwarder M, Alagesan K, Altmann F.

Methods Mol Biol. 2015;1321:427-35. doi: 10.1007/978-1-4939-2760-9_29.

PMID:
26082239
37.

Clinical Glycomics Employing Graphitized Carbon Liquid Chromatography-Mass Spectrometry.

Stavenhagen K, Kolarich D, Wuhrer M.

Chromatographia. 2015;78(5-6):307-320. Epub 2014 Dec 9. Review.

38.

The role of glycosylation in IBD.

Theodoratou E, Campbell H, Ventham NT, Kolarich D, Pučić-Baković M, Zoldoš V, Fernandes D, Pemberton IK, Rudan I, Kennedy NA, Wuhrer M, Nimmo E, Annese V, McGovern DP, Satsangi J, Lauc G.

Nat Rev Gastroenterol Hepatol. 2014 Oct;11(10):588-600. doi: 10.1038/nrgastro.2014.78. Epub 2014 Jun 10. Review.

PMID:
24912389
39.

MIRAGE: the minimum information required for a glycomics experiment.

York WS, Agravat S, Aoki-Kinoshita KF, McBride R, Campbell MP, Costello CE, Dell A, Feizi T, Haslam SM, Karlsson N, Khoo KH, Kolarich D, Liu Y, Novotny M, Packer NH, Paulson JC, Rapp E, Ranzinger R, Rudd PM, Smith DF, Struwe WB, Tiemeyer M, Wells L, Zaia J, Kettner C.

Glycobiology. 2014 May;24(5):402-6. doi: 10.1093/glycob/cwu018. Epub 2014 Mar 20.

40.

Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection.

Marco-Ramell A, Miller I, Nöbauer K, Möginger U, Segalés J, Razzazi-Fazeli E, Kolarich D, Bassols A.

J Proteomics. 2014 Apr 14;101:205-16. doi: 10.1016/j.jprot.2014.02.018. Epub 2014 Feb 24.

PMID:
24576640
41.

A study on Nim expression in Bacteroides fragilis.

Leitsch D, Sóki J, Kolarich D, Urbán E, Nagy E.

Microbiology. 2014 Mar;160(Pt 3):616-622. doi: 10.1099/mic.0.074807-0. Epub 2014 Jan 21.

42.

Trichomonas vaginalis flavin reductase 1 and its role in metronidazole resistance.

Leitsch D, Janssen BD, Kolarich D, Johnson PJ, Duchêne M.

Mol Microbiol. 2014 Jan;91(1):198-208. doi: 10.1111/mmi.12455. Epub 2013 Nov 20.

43.

Characterization and downstream mannose phosphorylation of human recombinant α-L-iduronidase produced in Arabidopsis complex glycan-deficient (cgl) seeds.

He X, Pierce O, Haselhorst T, von Itzstein M, Kolarich D, Packer NH, Gloster TM, Vocadlo DJ, Qian Y, Brooks D, Kermode AR.

Plant Biotechnol J. 2013 Dec;11(9):1034-43. doi: 10.1111/pbi.12096. Epub 2013 Jul 31.

44.

Bioanalysis Young Investigator Award 2013.

Saxena N, Kolarich D, Vivekanand Kulkarni M, Jankevics A, Benton C.

Bioanalysis. 2013 Jun;5(12):1479-84. doi: 10.4155/bio.13.97. No abstract available.

45.

Quantitative mapping of glycoprotein micro-heterogeneity and macro-heterogeneity: an evaluation of mass spectrometry signal strengths using synthetic peptides and glycopeptides.

Stavenhagen K, Hinneburg H, Thaysen-Andersen M, Hartmann L, Varón Silva D, Fuchser J, Kaspar S, Rapp E, Seeberger PH, Kolarich D.

J Mass Spectrom. 2013 Jun;48(6):i. doi: 10.1002/jms.3189.

PMID:
23776102
46.

Interlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012.

Leymarie N, Griffin PJ, Jonscher K, Kolarich D, Orlando R, McComb M, Zaia J, Aguilan J, Alley WR, Altmann F, Ball LE, Basumallick L, Bazemore-Walker CR, Behnken H, Blank MA, Brown KJ, Bunz SC, Cairo CW, Cipollo JF, Daneshfar R, Desaire H, Drake RR, Go EP, Goldman R, Gruber C, Halim A, Hathout Y, Hensbergen PJ, Horn DM, Hurum D, Jabs W, Larson G, Ly M, Mann BF, Marx K, Mechref Y, Meyer B, Möginger U, Neusüβ C, Nilsson J, Novotny MV, Nyalwidhe JO, Packer NH, Pompach P, Reiz B, Resemann A, Rohrer JS, Ruthenbeck A, Sanda M, Schulz JM, Schweiger-Hufnagel U, Sihlbom C, Song E, Staples GO, Suckau D, Tang H, Thaysen-Andersen M, Viner RI, An Y, Valmu L, Wada Y, Watson M, Windwarder M, Whittal R, Wuhrer M, Zhu Y, Zou C.

Mol Cell Proteomics. 2013 Oct;12(10):2935-51. doi: 10.1074/mcp.M113.030643. Epub 2013 Jun 13.

47.

Quantitative mapping of glycoprotein micro-heterogeneity and macro-heterogeneity: an evaluation of mass spectrometry signal strengths using synthetic peptides and glycopeptides.

Stavenhagen K, Hinneburg H, Thaysen-Andersen M, Hartmann L, Varón Silva D, Fuchser J, Kaspar S, Rapp E, Seeberger PH, Kolarich D.

J Mass Spectrom. 2013 Jun;48(6):627-39. doi: 10.1002/jms.3210.

PMID:
23722953
48.

Validation of the curation pipeline of UniCarb-DB: building a global glycan reference MS/MS repository.

Campbell MP, Nguyen-Khuong T, Hayes CA, Flowers SA, Alagesan K, Kolarich D, Packer NH, Karlsson NG.

Biochim Biophys Acta. 2014 Jan;1844(1 Pt A):108-16. doi: 10.1016/j.bbapap.2013.04.018. Epub 2013 Apr 25.

PMID:
23624262
49.

Structural feature ions for distinguishing N- and O-linked glycan isomers by LC-ESI-IT MS/MS.

Everest-Dass AV, Abrahams JL, Kolarich D, Packer NH, Campbell MP.

J Am Soc Mass Spectrom. 2013 Jun;24(6):895-906. doi: 10.1007/s13361-013-0610-4. Epub 2013 Apr 20.

PMID:
23605685
50.

Tandem mass spectra of glycan substructures enable the multistage mass spectrometric identification of determinants on oligosaccharides.

Everest-Dass AV, Kolarich D, Campbell MP, Packer NH.

Rapid Commun Mass Spectrom. 2013 May 15;27(9):931-9. doi: 10.1002/rcm.6527.

PMID:
23592194

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