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Mild maternal hyperglycemia in INS C93S transgenic pigs causes impaired glucose tolerance and metabolic alterations in neonatal offspring.

Renner S, Martins AS, Streckel E, Braun-Reichhart C, Backman M, Prehn C, Klymiuk N, Bähr A, Blutke A, Landbrecht-Schessl C, Wünsch A, Kessler B, Kurome M, Hinrichs A, Koopmans SJ, Krebs S, Kemter E, Rathkolb B, Nagashima H, Blum H, Ritzmann M, Wanke R, Aigner B, Adamski J, Hrabě de Angelis M, Wolf E.

Dis Model Mech. 2019 Aug 12;12(8). pii: dmm039156. doi: 10.1242/dmm.039156.


Author Correction: Consistent success in life-supporting porcine cardiac xenotransplantation.

Längin M, Mayr T, Reichart B, Michel S, Buchholz S, Guethoff S, Dashkevich A, Baehr A, Egerer S, Bauer A, Mihalj M, Panelli A, Issl L, Ying J, Fresch AK, Buttgereit I, Mokelke M, Radan J, Werner F, Lutzmann I, Steen S, Sjöberg T, Paskevicius A, Qiuming L, Sfriso R, Rieben R, Dahlhoff M, Kessler B, Kemter E, Kurome M, Zakhartchenko V, Klett K, Hinkel R, Kupatt C, Falkenau A, Reu S, Ellgass R, Herzog R, Binder U, Wich G, Skerra A, Ayares D, Kind A, Schönmann U, Kaup FJ, Hagl C, Wolf E, Klymiuk N, Brenner P, Abicht JM.

Nature. 2019 Apr;568(7752):E7. doi: 10.1038/s41586-019-1108-4.


Gene Editing in Primary Cells of Cattle and Pig.

Vochozkova P, Simmet K, Jemiller EM, Wünsch A, Klymiuk N.

Methods Mol Biol. 2019;1961:271-289. doi: 10.1007/978-1-4939-9170-9_17.


Animal models of arrhythmia: classic electrophysiology to genetically modified large animals.

Clauss S, Bleyer C, Schüttler D, Tomsits P, Renner S, Klymiuk N, Wakili R, Massberg S, Wolf E, Kääb S.

Nat Rev Cardiol. 2019 Aug;16(8):457-475. doi: 10.1038/s41569-019-0179-0. Review.


Consistent success in life-supporting porcine cardiac xenotransplantation.

Längin M, Mayr T, Reichart B, Michel S, Buchholz S, Guethoff S, Dashkevich A, Baehr A, Egerer S, Bauer A, Mihalj M, Panelli A, Issl L, Ying J, Fresch AK, Buttgereit I, Mokelke M, Radan J, Werner F, Lutzmann I, Steen S, Sjöberg T, Paskevicius A, Qiuming L, Sfriso R, Rieben R, Dahlhoff M, Kessler B, Kemter E, Kurome M, Zakhartchenko V, Klett K, Hinkel R, Kupatt C, Falkenau A, Reu S, Ellgass R, Herzog R, Binder U, Wich G, Skerra A, Ayares D, Kind A, Schönmann U, Kaup FJ, Hagl C, Wolf E, Klymiuk N, Brenner P, Abicht JM.

Nature. 2018 Dec;564(7736):430-433. doi: 10.1038/s41586-018-0765-z. Epub 2018 Dec 5. Erratum in: Nature. 2019 Apr;568(7752):E7.


Targeting αGal epitopes for multi-species embryo immunosurgery.

Kurome M, Baehr A, Simmet K, Jemiller EM, Egerer S, Dahlhoff M, Zakhartchenko V, Nagashima H, Klymiuk N, Kessler B, Wolf E.

Reprod Fertil Dev. 2019 Apr;31(4):820-826. doi: 10.1071/RD18120.


Early weaning completely eliminates porcine cytomegalovirus from a newly established pig donor facility for xenotransplantation.

Egerer S, Fiebig U, Kessler B, Zakhartchenko V, Kurome M, Reichart B, Kupatt C, Klymiuk N, Wolf E, Denner J, Bähr A.

Xenotransplantation. 2018 Jul;25(4):e12449. doi: 10.1111/xen.12449.


Remapping of the belted phenotype in cattle on BTA3 identifies a multiplication event as the candidate causal mutation.

Rothammer S, Kunz E, Krebs S, Bitzer F, Hauser A, Zinovieva N, Klymiuk N, Medugorac I.

Genet Sel Evol. 2018 Jul 6;50(1):36. doi: 10.1186/s12711-018-0407-9.


The mucus bundles responsible for airway cleaning are retained in cystic fibrosis and by cholinergic stimulation.

Ermund A, Meiss LN, Dolan B, Bähr A, Klymiuk N, Hansson GC.

Eur Respir J. 2018 Aug 30;52(2). pii: 1800457. doi: 10.1183/13993003.00457-2018. Print 2018 Aug.


Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood.

Abicht JM, Sfriso R, Reichart B, Längin M, Gahle K, Puga Yung GL, Seebach JD, Rieben R, Ayares D, Wolf E, Klymiuk N, Baehr A, Kind A, Mayr T, Bauer A.

Xenotransplantation. 2018 Sep;25(5):e12390. doi: 10.1111/xen.12390. Epub 2018 Mar 14.


OCT4/POU5F1 is required for NANOG expression in bovine blastocysts.

Simmet K, Zakhartchenko V, Philippou-Massier J, Blum H, Klymiuk N, Wolf E.

Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2770-2775. doi: 10.1073/pnas.1718833115. Epub 2018 Feb 26.


Granule-stored MUC5B mucins are packed by the non-covalent formation of N-terminal head-to-head tetramers.

Trillo-Muyo S, Nilsson HE, Recktenwald CV, Ermund A, Ridley C, Meiss LN, Bähr A, Klymiuk N, Wine JJ, Koeck PJB, Thornton DJ, Hebert H, Hansson GC.

J Biol Chem. 2018 Apr 13;293(15):5746-5754. doi: 10.1074/jbc.RA117.001014. Epub 2018 Feb 13.


Interspecies diversity of chloride channel regulators, calcium-activated 3 genes.

Mundhenk L, Erickson NA, Klymiuk N, Gruber AD.

PLoS One. 2018 Jan 18;13(1):e0191512. doi: 10.1371/journal.pone.0191512. eCollection 2018.


Modeling lethal X-linked genetic disorders in pigs with ensured fertility.

Matsunari H, Watanabe M, Nakano K, Enosawa S, Umeyama K, Uchikura A, Yashima S, Fukuda T, Klymiuk N, Kurome M, Kessler B, Wuensch A, Zakhartchenko V, Wolf E, Hanazono Y, Nagaya M, Umezawa A, Nakauchi H, Nagashima H.

Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):708-713. doi: 10.1073/pnas.1715940115. Epub 2018 Jan 8.


Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood.

Puga Yung G, Bongoni AK, Pradier A, Madelon N, Papaserafeim M, Sfriso R, Ayares DL, Wolf E, Klymiuk N, Bähr A, Constantinescu MA, Voegelin E, Kiermeir D, Jenni H, Rieben R, Seebach JD.

Xenotransplantation. 2018 Jan;25(1). doi: 10.1111/xen.12357. Epub 2017 Oct 22.


Photorhabdus luminescens lectin A (PllA): A new probe for detecting α-galactoside-terminating glycoconjugates.

Beshr G, Sikandar A, Jemiller EM, Klymiuk N, Hauck D, Wagner S, Wolf E, Koehnke J, Titz A.

J Biol Chem. 2017 Dec 1;292(48):19935-19951. doi: 10.1074/jbc.M117.812792. Epub 2017 Sep 28.


Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood.

Sfriso R, Bongoni A, Banz Y, Klymiuk N, Wolf E, Rieben R.

J Vis Exp. 2017 Sep 5;(127). doi: 10.3791/56227.


The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin.

Ermund A, Meiss LN, Rodriguez-Pineiro AM, Bähr A, Nilsson HE, Trillo-Muyo S, Ridley C, Thornton DJ, Wine JJ, Hebert H, Klymiuk N, Hansson GC.

Biochem Biophys Res Commun. 2017 Oct 21;492(3):331-337. doi: 10.1016/j.bbrc.2017.08.113. Epub 2017 Aug 30.


Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood.

Bongoni AK, Salvaris E, Nordling S, Klymiuk N, Wolf E, Ayares DL, Rieben R, Magnusson PU, Cowan PJ.

Sci Rep. 2017 Jun 30;7(1):4450. doi: 10.1038/s41598-017-04898-w.


LEA29Y expression in transgenic neonatal porcine islet-like cluster promotes long-lasting xenograft survival in humanized mice without immunosuppressive therapy.

Buerck LW, Schuster M, Oduncu FS, Baehr A, Mayr T, Guethoff S, Abicht J, Reichart B, Klymiuk N, Wolf E, Seissler J.

Sci Rep. 2017 Jun 15;7(1):3572. doi: 10.1038/s41598-017-03913-4.


Vessel Network Architecture of Adult Human Islets Promotes Distinct Cell-Cell Interactions In Situ and Is Altered After Transplantation.

Cohrs CM, Chen C, Jahn SR, Stertmann J, Chmelova H, Weitz J, Bähr A, Klymiuk N, Steffen A, Ludwig B, Kamvissi V, Wolf E, Bornstein SR, Solimena M, Speier S.

Endocrinology. 2017 May 1;158(5):1373-1385. doi: 10.1210/en.2016-1184.


Transgenic expression of human leukocyte antigen-E attenuates GalKO.hCD46 porcine lung xenograft injury.

Laird CT, Burdorf L, French BM, Kubicki N, Cheng X, Braileanu G, Sun W, O'Neill NA, Cimeno A, Parsell D, So E, Bähr A, Klymiuk N, Phelps CJ, Ayares D, Azimzadeh AM, Pierson RN.

Xenotransplantation. 2017 Mar;24(2). doi: 10.1111/xen.12294. Epub 2017 Mar 3.


Direct introduction of gene constructs into the pronucleus-like structure of cloned embryos: a new strategy for the generation of genetically modified pigs.

Kurome M, Leuchs S, Kessler B, Kemter E, Jemiller EM, Foerster B, Klymiuk N, Zakhartchenko V, Wolf E.

Transgenic Res. 2017 Apr;26(2):309-318. doi: 10.1007/s11248-016-0004-z. Epub 2016 Dec 10.


Progressive muscle proteome changes in a clinically relevant pig model of Duchenne muscular dystrophy.

Fröhlich T, Kemter E, Flenkenthaler F, Klymiuk N, Otte KA, Blutke A, Krause S, Walter MC, Wanke R, Wolf E, Arnold GJ.

Sci Rep. 2016 Sep 16;6:33362. doi: 10.1038/srep33362.


Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs.

Bähr A, Käser T, Kemter E, Gerner W, Kurome M, Baars W, Herbach N, Witter K, Wünsch A, Talker SC, Kessler B, Nagashima H, Saalmüller A, Schwinzer R, Wolf E, Klymiuk N.

PLoS One. 2016 May 13;11(5):e0155676. doi: 10.1371/journal.pone.0155676. eCollection 2016.


Transgenic Expression of Human Thrombomodulin Inhibits HMGB1-Induced Porcine Aortic Endothelial Cell Activation.

Bongoni AK, Klymiuk N, Wolf E, Ayares D, Rieben R, Cowan PJ.

Transplantation. 2016 Sep;100(9):1871-9. doi: 10.1097/TP.0000000000001188.


Chimeric 2C10R4 anti-CD40 antibody therapy is critical for long-term survival of GTKO.hCD46.hTBM pig-to-primate cardiac xenograft.

Mohiuddin MM, Singh AK, Corcoran PC, Thomas Iii ML, Clark T, Lewis BG, Hoyt RF, Eckhaus M, Pierson Iii RN, Belli AJ, Wolf E, Klymiuk N, Phelps C, Reimann KA, Ayares D, Horvath KA.

Nat Commun. 2016 Apr 5;7:11138. doi: 10.1038/ncomms11138.


Inhibition of complement component C5 prevents clotting in an ex vivo model of xenogeneic activation of coagulation.

Rataj D, Werwitzke S, Haarmeijer B, Winkler M, Ramackers W, Petersen B, Niemann H, Wünsch A, Bähr A, Klymiuk N, Wolf E, Abicht JM, Ayares D, Tiede A.

Xenotransplantation. 2016 Mar;23(2):117-27. doi: 10.1111/xen.12218. Epub 2016 Jan 16.


Pre-clinical heterotopic intrathoracic heart xenotransplantation: a possibly useful clinical technique.

Abicht JM, Mayr T, Reichart B, Buchholz S, Werner F, Lutzmann I, Schmoeckel M, Bauer A, Thormann M, Langenmayer M, Herbach N, Pohla H, Herzog R, McGregor CG, Ayares D, Wolf E, Klymiuk N, Baehr A, Kind A, Hagl C, Ganswindt U, Belka C, Guethoff S, Brenner P.

Xenotransplantation. 2015 Nov-Dec;22(6):427-42. doi: 10.1111/xen.12213. Epub 2015 Nov 25.


Computed Tomography (CT) Scanning Facilitates Early Identification of Neonatal Cystic Fibrosis Piglets.

Guillon A, Chevaleyre C, Barc C, Berri M, Adriaensen H, Lecompte F, Villemagne T, Pezant J, Delaunay R, Moënne-Loccoz J, Berthon P, Bähr A, Wolf E, Klymiuk N, Attucci S, Ramphal R, Sarradin P, Buzoni-Gatel D, Si-Tahar M, Caballero I.

PLoS One. 2015 Nov 23;10(11):e0143459. doi: 10.1371/journal.pone.0143459. eCollection 2015.


Tailored Pig Models for Preclinical Efficacy and Safety Testing of Targeted Therapies.

Klymiuk N, Seeliger F, Bohlooly-Y M, Blutke A, Rudmann DG, Wolf E.

Toxicol Pathol. 2016 Apr;44(3):346-57. doi: 10.1177/0192623315609688. Epub 2015 Oct 27. Review.


Engraftment and reversal of diabetes after intramuscular transplantation of neonatal porcine islet-like clusters.

Wolf-van Buerck L, Schuster M, Baehr A, Mayr T, Guethoff S, Abicht J, Reichart B, Nam-Apostolopoulos YC, Klymiuk N, Wolf E, Seissler J.

Xenotransplantation. 2015 Nov-Dec;22(6):443-50. doi: 10.1111/xen.12201. Epub 2015 Oct 21.


Naturally Occurring Deletion Mutants of the Pig-Specific, Intestinal Crypt Epithelial Cell Protein CLCA4b without Apparent Phenotype.

Plog S, Klymiuk N, Binder S, Van Hook MJ, Thoreson WB, Gruber AD, Mundhenk L.

PLoS One. 2015 Oct 16;10(10):e0140050. doi: 10.1371/journal.pone.0140050. eCollection 2015.


Transgenic Expression of Human CD46 on Porcine Endothelium: Effect on Coagulation and Fibrinolytic Cascades During Ex Vivo Human-to-Pig Limb Xenoperfusions.

Bongoni AK, Kiermeir D, Schnider J, Jenni H, Garimella P, Bähr A, Klymiuk N, Wolf E, Ayares D, Voegelin E, Constantinescu MA, Seebach JD, Rieben R.

Transplantation. 2015 Oct;99(10):2061-9. doi: 10.1097/TP.0000000000000746.


Virus safety of islet cell transplantation from transgenic pigs to marmosets.

Plotzki E, Wolf-van Buerck L, Knauf Y, Becker T, Maetz-Rensing K, Schuster M, Baehr A, Klymiuk N, Wolf E, Seissler J, Denner J.

Virus Res. 2015 Jun 2;204:95-102. doi: 10.1016/j.virusres.2015.04.016. Epub 2015 May 6.


Pig-to-baboon heterotopic heart transplantation--exploratory preliminary experience with pigs transgenic for human thrombomodulin and comparison of three costimulation blockade-based regimens.

Iwase H, Ekser B, Satyananda V, Bhama J, Hara H, Ezzelarab M, Klein E, Wagner R, Long C, Thacker J, Li J, Zhou H, Jiang M, Nagaraju S, Zhou H, Veroux M, Bajona P, Wijkstrom M, Wang Y, Phelps C, Klymiuk N, Wolf E, Ayares D, Cooper DK.

Xenotransplantation. 2015 May-Jun;22(3):211-20. doi: 10.1111/xen.12167. Epub 2015 Apr 3.


Xenotransplantation of porcine islet cells as a potential option for the treatment of type 1 diabetes in the future.

Reichart B, Niemann H, Chavakis T, Denner J, Jaeckel E, Ludwig B, Marckmann G, Schnieke A, Schwinzer R, Seissler J, Tönjes RR, Klymiuk N, Wolf E, Bornstein SR.

Horm Metab Res. 2015 Jan;47(1):31-5. doi: 10.1055/s-0034-1395518. Epub 2014 Dec 15. Review.


Complement dependent early immunological responses during ex vivo xenoperfusion of hCD46/HLA-E double transgenic pig forelimbs with human blood.

Bongoni AK, Kiermeir D, Jenni H, Bähr A, Ayares D, Klymiuk N, Wolf E, Voegelin E, Constantinescu MA, Seebach JD, Rieben R.

Xenotransplantation. 2014 May-Jun;21(3):230-43. doi: 10.1111/xen.12090. Epub 2014 Mar 17.


Homologous recombination contributes to the repair of zinc-finger-nuclease induced double strand breaks in pig primary cells and facilitates recombination with exogenous DNA.

Klymiuk N, Fezert P, Wünsch A, Kurome M, Kessler B, Wolf E.

J Biotechnol. 2014 May 10;177:74-81. doi: 10.1016/j.jbiotec.2014.01.018. Epub 2014 Mar 5.


One-year heterotopic cardiac xenograft survival in a pig to baboon model.

Mohiuddin MM, Singh AK, Corcoran PC, Hoyt RF, Thomas ML 3rd, Lewis BG, Eckhaus M, Reimann KA, Klymiuk N, Wolf E, Ayares D, Horvath KA.

Am J Transplant. 2014 Feb;14(2):488-9. doi: 10.1111/ajt.12562. Epub 2013 Dec 11. No abstract available.


Regulatory sequences of the porcine THBD gene facilitate endothelial-specific expression of bioactive human thrombomodulin in single- and multitransgenic pigs.

Wuensch A, Baehr A, Bongoni AK, Kemter E, Blutke A, Baars W, Haertle S, Zakhartchenko V, Kurome M, Kessler B, Faber C, Abicht JM, Reichart B, Wanke R, Schwinzer R, Nagashima H, Rieben R, Ayares D, Wolf E, Klymiuk N.

Transplantation. 2014 Jan 27;97(2):138-47. doi: 10.1097/TP.0b013e3182a95cbc.


Activation of the lectin pathway of complement in pig-to-human xenotransplantation models.

Bongoni AK, Kiermeir D, Jenni H, Wünsch A, Bähr A, Ayares D, Seebach JD, Wolf E, Klymiuk N, Constantinescu MA, Vögelin E, Rieben R.

Transplantation. 2013 Nov 15;96(9):791-9. doi: 10.1097/TP.0b013e3182a3a52b.


Dystrophin-deficient pigs provide new insights into the hierarchy of physiological derangements of dystrophic muscle.

Klymiuk N, Blutke A, Graf A, Krause S, Burkhardt K, Wuensch A, Krebs S, Kessler B, Zakhartchenko V, Kurome M, Kemter E, Nagashima H, Schoser B, Herbach N, Blum H, Wanke R, Aartsma-Rus A, Thirion C, Lochmüller H, Walter MC, Wolf E.

Hum Mol Genet. 2013 Nov 1;22(21):4368-82. doi: 10.1093/hmg/ddt287. Epub 2013 Jun 19.


Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set.

Kurome M, Geistlinger L, Kessler B, Zakhartchenko V, Klymiuk N, Wuensch A, Richter A, Baehr A, Kraehe K, Burkhardt K, Flisikowski K, Flisikowska T, Merkl C, Landmann M, Durkovic M, Tschukes A, Kraner S, Schindelhauer D, Petri T, Kind A, Nagashima H, Schnieke A, Zimmer R, Wolf E.

BMC Biotechnol. 2013 May 20;13:43. doi: 10.1186/1472-6750-13-43.


Permanent neonatal diabetes in INS(C94Y) transgenic pigs.

Renner S, Braun-Reichhart C, Blutke A, Herbach N, Emrich D, Streckel E, Wünsch A, Kessler B, Kurome M, Bähr A, Klymiuk N, Krebs S, Puk O, Nagashima H, Graw J, Blum H, Wanke R, Wolf E.

Diabetes. 2013 May;62(5):1505-11. doi: 10.2337/db12-1065. Epub 2012 Dec 28.


Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig.

Richter A, Kurome M, Kessler B, Zakhartchenko V, Klymiuk N, Nagashima H, Wolf E, Wuensch A.

BMC Biotechnol. 2012 Nov 9;12:84. doi: 10.1186/1472-6750-12-84.


Xenografted islet cell clusters from INSLEA29Y transgenic pigs rescue diabetes and prevent immune rejection in humanized mice.

Klymiuk N, van Buerck L, Bähr A, Offers M, Kessler B, Wuensch A, Kurome M, Thormann M, Lochner K, Nagashima H, Herbach N, Wanke R, Seissler J, Wolf E.

Diabetes. 2012 Jun;61(6):1527-32. doi: 10.2337/db11-1325. Epub 2012 Apr 20.


The porcine chloride channel calcium-activated family member pCLCA4a mirrors lung expression of the human hCLCA4.

Plog S, Grötzsch T, Klymiuk N, Kobalz U, Gruber AD, Mundhenk L.

J Histochem Cytochem. 2012 Jan;60(1):45-56. doi: 10.1369/0022155411426455.


Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis.

Klymiuk N, Mundhenk L, Kraehe K, Wuensch A, Plog S, Emrich D, Langenmayer MC, Stehr M, Holzinger A, Kröner C, Richter A, Kessler B, Kurome M, Eddicks M, Nagashima H, Heinritzi K, Gruber AD, Wolf E.

J Mol Med (Berl). 2012 May;90(5):597-608. doi: 10.1007/s00109-011-0839-y. Epub 2011 Dec 15.


First inducible transgene expression in porcine large animal models.

Klymiuk N, Böcker W, Schönitzer V, Bähr A, Radic T, Fröhlich T, Wünsch A, Keßler B, Kurome M, Schilling E, Herbach N, Wanke R, Nagashima H, Mutschler W, Arnold GJ, Schwinzer R, Schieker M, Wolf E.

FASEB J. 2012 Mar;26(3):1086-99. doi: 10.1096/fj.11-185041. Epub 2011 Dec 1.


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