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J Clin Microbiol. 2016 Mar;54(3):785-7. doi: 10.1128/JCM.02850-15. Epub 2015 Dec 30.

Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens.

Author information

1
University of Utah Department of Medicine, Division of Infectious Diseases, School of Medicine, Salt Lake City, Utah, USA University of Utah Department of Pathology, Clinical Microbiology, Salt Lake City, Utah, USA kim.hanson@hsc.utah.edu anne.blaschke@hsc.utah.edu.
2
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
3
University of Utah Department of Pediatrics, Division of Pediatric Infectious Diseases, Salt Lake City, Utah, USA.
4
Intermountain Primary Children's Hospital, Salt Lake City, Utah, USA.
5
University of Utah Department of Pathology, Clinical Microbiology, Salt Lake City, Utah, USA Intermountain Primary Children's Hospital, Salt Lake City, Utah, USA.
6
BioFire Diagnostics, LLC, Salt Lake City, Utah, USA.
7
University of Utah Department of Pediatrics, Division of Pediatric Infectious Diseases, Salt Lake City, Utah, USA kim.hanson@hsc.utah.edu anne.blaschke@hsc.utah.edu.

Abstract

We evaluated a multiplexed PCR panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. Panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. Overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively.

PMID:
26719436
PMCID:
PMC4767945
DOI:
10.1128/JCM.02850-15
[Indexed for MEDLINE]
Free PMC Article

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