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Items: 1 to 50 of 162

1.

Direct measurement of the kinetics of CBM9 fusion-tag bioprocessing using luminescence resonance energy transfer.

Kavoosi M, Creagh AL, Turner RF, Kilburn DG, Haynes CA.

Biotechnol Prog. 2009 May-Jun;25(3):874-81. doi: 10.1002/btpr.88.

PMID:
19496182
2.

Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture.

Gorenflo VM, Pfeifer TA, Lesnicki G, Kwan EM, Grigliatti TA, Kilburn DG, Piret JM.

Cytotechnology. 2004 Mar;44(3):93-102. doi: 10.1007/s10616-005-0703-4.

3.

On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris.

Guarna MM, Lesnicki GJ, Tam BM, Robinson J, Radziminski CZ, Hasenwinkle D, Boraston A, Jervis E, MacGillivray RT, Turner RF, Kilburn DG.

Biotechnol Bioeng. 1997 Nov 5;56(3):279-86. doi: 10.1002/(SICI)1097-0290(19971105)56:3<279::AID-BIT5>3.0.CO;2-G.

PMID:
18636643
4.

Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system.

Hasenwinkle D, Jervis E, Kops O, Liu C, Lesnicki G, Haynes CA, Kilburn DG.

Biotechnol Bioeng. 1997 Sep 20;55(6):854-63. doi: 10.1002/(SICI)1097-0290(19970920)55:6<854::AID-BIT4>3.0.CO;2-F.

PMID:
18636596
5.

Mechanically stable porous cellulose media for affinity purification of family 9 cellulose-binding module-tagged fusion proteins.

Kavoosi M, Lam D, Bryan J, Kilburn DG, Haynes CA.

J Chromatogr A. 2007 Dec 21;1175(2):187-96. Epub 2007 Aug 7.

PMID:
18023448
6.
7.

Strategy for selecting and characterizing linker peptides for CBM9-tagged fusion proteins expressed in Escherichia coli.

Kavoosi M, Creagh AL, Kilburn DG, Haynes CA.

Biotechnol Bioeng. 2007 Oct 15;98(3):599-610.

PMID:
17394253
8.

N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation.

Kwan EM, Boraston AB, McLean BW, Kilburn DG, Warren RA.

Protein Eng Des Sel. 2005 Oct;18(10):497-501. Epub 2005 Sep 9.

PMID:
16155117
9.

Dynamic localization and persistent stimulation of factor-dependent cells by a stem cell factor / cellulose binding domain fusion protein.

Jervis EJ, Guarna MM, Doheny JG, Haynes CA, Kilburn DG.

Biotechnol Bioeng. 2005 Aug 5;91(3):314-24.

PMID:
15948140
10.

Inexpensive and generic affinity purification of recombinant proteins using a family 2a CBM fusion tag.

Rodriguez B, Kavoosi M, Koska J, Creagh AL, Kilburn DG, Haynes CA.

Biotechnol Prog. 2004 Sep-Oct;20(5):1479-89.

PMID:
15458333
11.

Inexpensive one-step purification of polypeptides expressed in Escherichia coli as fusions with the family 9 carbohydrate-binding module of xylanase 10A from T. maritima.

Kavoosi M, Meijer J, Kwan E, Creagh AL, Kilburn DG, Haynes CA.

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 25;807(1):87-94.

PMID:
15177165
12.

O-glycosylation of a recombinant carbohydrate-binding module mutant secreted by Pichia pastoris.

Boraston AB, Sandercock L, Warren RA, Kilburn DG.

J Mol Microbiol Biotechnol. 2003;5(1):29-36.

PMID:
12673059
13.

Structure and ligand binding of carbohydrate-binding module CsCBM6-3 reveals similarities with fucose-specific lectins and "galactose-binding" domains.

Boraston AB, Notenboom V, Warren RA, Kilburn DG, Rose DR, Davies G.

J Mol Biol. 2003 Mar 28;327(3):659-69.

PMID:
12634060
14.

Effects of methanol concentration on expression levels of recombinant protein in fed-batch cultures of Pichia methanolica.

Mayson BE, Kilburn DG, Zamost BL, Raymond CK, Lesnicki GJ.

Biotechnol Bioeng. 2003 Feb 5;81(3):291-8.

PMID:
12474251
15.

Recognition and hydrolysis of noncrystalline cellulose.

Boraston AB, Kwan E, Chiu P, Warren RA, Kilburn DG.

J Biol Chem. 2003 Feb 21;278(8):6120-7. Epub 2002 Nov 8.

16.

Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties.

Kwan E, Guarna MM, Boraston AB, Gilkes NR, Haynes CA, Kilburn DG, Warren RA.

Biotechnol Bioeng. 2002 Sep 30;79(7):724-32.

PMID:
12209795
17.

Carbohydrate-binding modules recognize fine substructures of cellulose.

McLean BW, Boraston AB, Brouwer D, Sanaie N, Fyfe CA, Warren RA, Kilburn DG, Haynes CA.

J Biol Chem. 2002 Dec 27;277(52):50245-54. Epub 2002 Aug 20.

18.

Differential oligosaccharide recognition by evolutionarily-related beta-1,4 and beta-1,3 glucan-binding modules.

Boraston AB, Nurizzo D, Notenboom V, Ducros V, Rose DR, Kilburn DG, Davies GJ.

J Mol Biol. 2002 Jun 21;319(5):1143-56.

PMID:
12079353
19.

Do cellulose binding domains increase substrate accessibility?

Esteghlalian AR, Srivastava V, Gilkes NR, Kilburn DG, Warren RA, Saddle JN.

Appl Biochem Biotechnol. 2001 Spring;91-93:575-92.

PMID:
11963886
20.
21.

Co-operative binding of triplicate carbohydrate-binding modules from a thermophilic xylanase.

Boraston AB, McLean BW, Chen G, Li A, Warren RA, Kilburn DG.

Mol Microbiol. 2002 Jan;43(1):187-94.

22.

Identification and glucan-binding properties of a new carbohydrate-binding module family.

Boraston AB, Ghaffari M, Warren RA, Kilburn DG.

Biochem J. 2002 Jan 1;361(Pt 1):35-40.

23.

Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study.

Notenboom V, Boraston AB, Chiu P, Freelove AC, Kilburn DG, Rose DR.

J Mol Biol. 2001 Dec 7;314(4):797-806.

PMID:
11733998
24.

beta-1,3-Glucan binding by a thermostable carbohydrate-binding module from Thermotoga maritima.

Boraston AB, Warren RA, Kilburn DG.

Biochemistry. 2001 Dec 4;40(48):14679-85.

PMID:
11724582
25.

Expression analysis of a modified factor X in stably transformed insect cell lines.

Pfeifer TA, Guarna MM, Kwan EM, Lesnicki G, Theilmann DA, Grigliatti TA, Kilburn DG.

Protein Expr Purif. 2001 Nov;23(2):233-41.

PMID:
11676597
27.

Crystal structures of the family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A in native and ligand-bound forms.

Notenboom V, Boraston AB, Kilburn DG, Rose DR.

Biochemistry. 2001 May 29;40(21):6248-56.

PMID:
11371186
28.

Binding specificity and thermodynamics of a family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A.

Boraston AB, Creagh AL, Alam MM, Kormos JM, Tomme P, Haynes CA, Warren RA, Kilburn DG.

Biochemistry. 2001 May 29;40(21):6240-7.

PMID:
11371185
29.

A family 2a carbohydrate-binding module suitable as an affinity tag for proteins produced in Pichia pastoris.

Boraston AB, McLean BW, Guarna MM, Amandaron-Akow E, Kilburn DG.

Protein Expr Purif. 2001 Apr;21(3):417-23.

PMID:
11281716
30.
31.

Factor X fusion proteins: improved production and use in the release in vitro of biologically active hirudin from an inactive alpha-factor-hirudin fusion protein.

Guarna MM, Côté HC, Kwan EM, Rintoul GL, Meyhack B, Heim J, MacGillivray RT, Warren RA, Kilburn DG.

Protein Expr Purif. 2000 Nov;20(2):133-41.

PMID:
11049737
32.

Specificity and affinity of substrate binding by a family 17 carbohydrate-binding module from Clostridium cellulovorans cellulase 5A.

Boraston AB, Chiu P, Warren RA, Kilburn DG.

Biochemistry. 2000 Sep 12;39(36):11129-36.

PMID:
10998252
33.

Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules.

Tomme P, Boraston A, Kormos JM, Warren RA, Kilburn DG.

Enzyme Microb Technol. 2000 Oct 1;27(7):453-458.

PMID:
10978766
34.

A novel mechanism of xylan binding by a lectin-like module from Streptomyces lividans xylanase 10A.

Boraston AB, Tomme P, Amandoron EA, Kilburn DG.

Biochem J. 2000 Sep 15;350 Pt 3:933-41.

35.

Binding site analysis of cellulose binding domain CBD(N1) from endoglucanse C of Cellulomonas fimi by site-directed mutagenesis.

Kormos J, Johnson PE, Brun E, Tomme P, McIntosh LP, Haynes CA, Kilburn DG.

Biochemistry. 2000 Aug 1;39(30):8844-52.

PMID:
10913296
37.

Mannanase Man26A from Cellulomonas fimi has a mannan-binding module.

Stoll D, Boraston A, Stålbrand H, McLean BW, Kilburn DG, Warren RA.

FEMS Microbiol Lett. 2000 Feb 15;183(2):265-9.

38.
39.

Concentration-dependent internalization of a cytokine/cytokine receptor complex in human hematopoietic cells.

Zandstra PW, Jervis E, Haynes CA, Kilburn DG, Eaves CJ, Piret JM.

Biotechnol Bioeng. 1999 May 20;63(4):493-501.

PMID:
10099630
40.

Relationship between recombinant activated protein C secretion rates and mRNA levels in baby hamster kidney cells.

Fann CH, Guarna MM, Kilburn DG, Piret JM.

Biotechnol Bioeng. 1999 May 20;63(4):464-72.

PMID:
10099627
41.

Characterization and affinity applications of cellulose-binding domains.

Tomme P, Boraston A, McLean B, Kormos J, Creagh AL, Sturch K, Gilkes NR, Haynes CA, Warren RA, Kilburn DG.

J Chromatogr B Biomed Sci Appl. 1998 Sep 11;715(1):283-96.

PMID:
9792516
42.

Analysis of molecular size distributions of cellulose molecules during hydrolysis of cellulose by recombinant Cellulomonas fimi beta-1,4-glucanases.

Stålbrand H, Mansfield SD, Saddler JN, Kilburn DG, Warren RA, Gilkes NR.

Appl Environ Microbiol. 1998 Jul;64(7):2374-9.

43.

Stability and oligosaccharide binding of the N1 cellulose-binding domain of Cellulomonas fimi endoglucanase CenC.

Creagh AL, Koska J, Johnson PE, Tomme P, Joshi MD, McIntosh LP, Kilburn DG, Haynes CA.

Biochemistry. 1998 Mar 10;37(10):3529-37.

PMID:
9521674
44.

Surface diffusion of cellulases and their isolated binding domains on cellulose.

Jervis EJ, Haynes CA, Kilburn DG.

J Biol Chem. 1997 Sep 19;272(38):24016-23.

45.

Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by nuclear magnetic resonance spectroscopy.

Johnson PE, Joshi MD, Tomme P, Kilburn DG, McIntosh LP.

Biochemistry. 1996 Nov 12;35(45):14381-94.

PMID:
8916925
46.
47.

Probing the role of tryptophan residues in a cellulose-binding domain by chemical modification.

Bray MR, Johnson PE, Gilkes NR, McIntosh LP, Kilburn DG, Warren RA.

Protein Sci. 1996 Nov;5(11):2311-8.

48.

Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven.

Creagh AL, Ong E, Jervis E, Kilburn DG, Haynes CA.

Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12229-34.

49.

Cellulose-binding domains. Versatile affinity tags for inexpensive large-scale purification, concentration, and immobilization of fusion proteins.

Tomme P, Gilkes NR, Guarna MM, Haynes CA, Hasenwinkle D, Jervis E, Johnson P, McIntosh L, Warren RA, Kilburn DG.

Ann N Y Acad Sci. 1996 Oct 12;799:418-24. No abstract available.

PMID:
8958104
50.

Engineering factor X fusions for expression in Pichia pastoris.

Guarna MM, Côté HC, Amandoron EA, MacGillivray RT, Warren RA, Kilburn DG.

Ann N Y Acad Sci. 1996 Oct 12;799:397-400. No abstract available.

PMID:
8958102

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