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Items: 8

1.

A pipeline for characterization of novel Cas9 orthologs.

Karvelis T, Young JK, Siksnys V.

Methods Enzymol. 2019;616:219-240. doi: 10.1016/bs.mie.2018.10.021. Epub 2018 Dec 27.

PMID:
30691644
2.

Harnessing the natural diversity and in vitro evolution of Cas9 to expand the genome editing toolbox.

Karvelis T, Gasiunas G, Siksnys V.

Curr Opin Microbiol. 2017 Jun;37:88-94. doi: 10.1016/j.mib.2017.05.009. Epub 2017 Jun 20. Review.

PMID:
28645099
3.

Methods for decoding Cas9 protospacer adjacent motif (PAM) sequences: A brief overview.

Karvelis T, Gasiunas G, Siksnys V.

Methods. 2017 May 15;121-122:3-8. doi: 10.1016/j.ymeth.2017.03.006. Epub 2017 Mar 24. Review.

PMID:
28344037
4.

Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements.

Karvelis T, Gasiunas G, Young J, Bigelyte G, Silanskas A, Cigan M, Siksnys V.

Genome Biol. 2015 Nov 19;16:253. doi: 10.1186/s13059-015-0818-7.

5.

Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex.

Glemzaite M, Balciunaite E, Karvelis T, Gasiunas G, Grusyte MM, Alzbutas G, Jurcyte A, Anderson EM, Maksimova E, Smith AJ, Lubys A, Zaliauskiene L, Siksnys V.

RNA Biol. 2015;12(1):1-4. doi: 10.1080/15476286.2015.1017209. No abstract available.

6.

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

Szczelkun MD, Tikhomirova MS, Sinkunas T, Gasiunas G, Karvelis T, Pschera P, Siksnys V, Seidel R.

Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9798-803. doi: 10.1073/pnas.1402597111. Epub 2014 May 27.

7.

Programmable DNA cleavage in vitro by Cas9.

Karvelis T, Gasiunas G, Siksnys V.

Biochem Soc Trans. 2013 Dec;41(6):1401-6. doi: 10.1042/BST20130164. Review.

PMID:
24256227
8.

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus.

Karvelis T, Gasiunas G, Miksys A, Barrangou R, Horvath P, Siksnys V.

RNA Biol. 2013 May;10(5):841-51. doi: 10.4161/rna.24203. Epub 2013 Mar 27.

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