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Infect Immun. 2018 Dec 19;87(1). pii: e00939-17. doi: 10.1128/IAI.00939-17. Print 2019 Jan.

Peptidyl-Prolyl-cis/trans-Isomerases Mip and PpiB of Legionella pneumophila Contribute to Surface Translocation, Growth at Suboptimal Temperature, and Infection.

Author information

1
Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany.
2
Abteilung für Herz-, Thorax- und Gefäßchirurgie, Städtisches Klinikum Braunschweig gGmbH, Braunschweig, Germany.
3
Institut für Psychologie, TU Braunschweig, Technische Universität Braunschweig, Braunschweig, Germany.
4
Klinik für Allgemein-, Viszeral- und Thoraxchirurgie, Allgemeines Krankenhaus Celle, Celle, Germany.
5
Institut für Pathologie, Städtisches Klinikum Braunschweig gGmbH, Braunschweig, Germany.
6
Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany m.steinert@tu-bs.de.
7
Helmholtz Centre for Infection Research, Braunschweig, Germany.

Abstract

The gammaproteobacterium Legionella pneumophila is the causative agent of Legionnaires' disease, an atypical pneumonia that manifests itself with severe lung damage. L. pneumophila, a common inhabitant of freshwater environments, replicates in free-living amoebae and persists in biofilms in natural and man-made water systems. Its environmental versatility is reflected in its ability to survive and grow within a broad temperature range as well as its capability to colonize and infect a wide range of hosts, including protozoa and humans. Peptidyl-prolyl-cis/trans-isomerases (PPIases) are multifunctional proteins that are mainly involved in protein folding and secretion in bacteria. In L. pneumophila the surface-associated PPIase Mip was shown to facilitate the establishment of the intracellular infection cycle in its early stages. The cytoplasmic PpiB was shown to promote cold tolerance. Here, we set out to analyze the interrelationship of these two relevant PPIases in the context of environmental fitness and infection. We demonstrate that the PPIases Mip and PpiB are important for surfactant-dependent sliding motility and adaptation to suboptimal temperatures, features that contribute to the environmental fitness of L. pneumophila Furthermore, they contribute to infection of the natural host Acanthamoeba castellanii as well as human macrophages and human explanted lung tissue. These effects were additive in the case of sliding motility or synergistic in the case of temperature tolerance and infection, as assessed by the behavior of the double mutant. Accordingly, we propose that Mip and PpiB are virulence modulators of L. pneumophila with compensatory action and pleiotropic effects.

KEYWORDS:

Legionella pneumophila ; PpiB; human lung tissue explants; intracellular infection; macrophage infectivity potentiator; motility; peptidyl-prolyl cis/trans isomerase; sliding; temperature adaptation

PMID:
30323027
PMCID:
PMC6300627
DOI:
10.1128/IAI.00939-17
[Indexed for MEDLINE]
Free PMC Article

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