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Items: 1 to 50 of 440

1.

It takes two to tango: The dance of the permease.

Kaback HR, Guan L.

J Gen Physiol. 2019 May 30. pii: jgp.201912377. doi: 10.1085/jgp.201912377. [Epub ahead of print] Review.

PMID:
31147449
2.

Insertion and folding pathways of single membrane proteins guided by translocases and insertases.

Serdiuk T, Steudle A, Mari SA, Manioglu S, Kaback HR, Kuhn A, Müller DJ.

Sci Adv. 2019 Jan 30;5(1):eaau6824. doi: 10.1126/sciadv.aau6824. eCollection 2019 Jan.

3.

Arg302 governs the pKa of Glu325 in LacY.

Grytsyk N, Santos Seiça AF, Sugihara J, Kaback HR, Hellwig P.

Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):4934-4939. doi: 10.1073/pnas.1820744116. Epub 2019 Feb 21.

PMID:
30792352
4.

Engineered occluded apo-intermediate of LacY.

Smirnova I, Kasho V, Kaback HR.

Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):12716-12721. doi: 10.1073/pnas.1816267115. Epub 2018 Nov 26.

5.

Crystal Structure of a ligand-bound LacY-Nanobody Complex.

Kumar H, Finer-Moore JS, Jiang X, Smirnova I, Kasho V, Pardon E, Steyaert J, Kaback HR, Stroud RM.

Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):8769-8774. doi: 10.1073/pnas.1801774115. Epub 2018 Aug 14.

6.

Oversized galactosides as a probe for conformational dynamics in LacY.

Smirnova I, Kasho V, Jiang X, Chen HM, Withers SG, Kaback HR.

Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):4146-4151. doi: 10.1073/pnas.1800706115. Epub 2018 Mar 30.

7.

An Asymmetric Conformational Change in LacY.

Smirnova I, Kasho V, Jiang X, Kaback HR.

Biochemistry. 2017 Apr 4;56(13):1943-1950. doi: 10.1021/acs.biochem.7b00134. Epub 2017 Mar 23.

8.

Quantification of Detergents Complexed with Membrane Proteins.

Chaptal V, Delolme F, Kilburg A, Magnard S, Montigny C, Picard M, Prier C, Monticelli L, Bornert O, Agez M, Ravaud S, Orelle C, Wagner R, Jawhari A, Broutin I, Pebay-Peyroula E, Jault JM, Kaback HR, le Maire M, Falson P.

Sci Rep. 2017 Feb 8;7:41751. doi: 10.1038/srep41751.

9.

pKa of Glu325 in LacY.

Grytsyk N, Sugihara J, Kaback HR, Hellwig P.

Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1530-1535. doi: 10.1073/pnas.1621431114. Epub 2017 Feb 1.

10.

Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.

Jiang X, Smirnova I, Kasho V, Wu J, Hirata K, Ke M, Pardon E, Steyaert J, Yan N, Kaback HR.

Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12420-12425. Epub 2016 Oct 19.

11.

YidC assists the stepwise and stochastic folding of membrane proteins.

Serdiuk T, Balasubramaniam D, Sugihara J, Mari SA, Kaback HR, Müller DJ.

Nat Chem Biol. 2016 Nov;12(11):911-917. doi: 10.1038/nchembio.2169. Epub 2016 Sep 5.

12.

Thermodynamics of Nanobody Binding to Lactose Permease.

Hariharan P, Andersson M, Jiang X, Pardon E, Steyaert J, Kaback HR, Guan L.

Biochemistry. 2016 Oct 25;55(42):5917-5926. doi: 10.1021/acs.biochem.6b00826. Epub 2016 Oct 12.

13.

Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY.

Jiang X, Andersson M, Chau BT, Wong LY, Villafuerte MK, Kaback HR.

Biochemistry. 2016 Aug 9;55(31):4326-32. doi: 10.1021/acs.biochem.6b00666. Epub 2016 Aug 1.

14.

pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases.

Bazzone A, Madej MG, Kaback HR, Fendler K.

PLoS One. 2016 May 26;11(5):e0156392. doi: 10.1371/journal.pone.0156392. eCollection 2016.

15.

Transient conformers of LacY are trapped by nanobodies.

Smirnova I, Kasho V, Jiang X, Pardon E, Steyaert J, Kaback HR.

Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):13839-44. doi: 10.1073/pnas.1519485112. Epub 2015 Oct 28. Erratum in: Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):E101.

16.

Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site.

Kumar H, Finer-Moore JS, Kaback HR, Stroud RM.

Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9004-9. doi: 10.1073/pnas.1509854112. Epub 2015 Jul 8.

17.

Observing a lipid-dependent alteration in single lactose permeases.

Serdiuk T, Sugihara J, Mari SA, Kaback HR, Müller DJ.

Structure. 2015 Apr 7;23(4):754-61. doi: 10.1016/j.str.2015.02.009. Epub 2015 Mar 19.

18.

Thermodynamic mechanism for inhibition of lactose permease by the phosphotransferase protein IIAGlc.

Hariharan P, Balasubramaniam D, Peterkofsky A, Kaback HR, Guan L.

Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2407-12. doi: 10.1073/pnas.1500891112. Epub 2015 Feb 9.

19.

A chemiosmotic mechanism of symport.

Kaback HR.

Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1259-64. doi: 10.1073/pnas.1419325112. Epub 2015 Jan 7.

20.

Outward-facing conformers of LacY stabilized by nanobodies.

Smirnova I, Kasho V, Jiang X, Pardon E, Steyaert J, Kaback HR.

Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18548-53. doi: 10.1073/pnas.1422265112. Epub 2014 Dec 15.

21.

Real-time conformational changes in LacY.

Smirnova I, Kasho V, Kaback HR.

Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8440-5. doi: 10.1073/pnas.1408374111. Epub 2014 May 28.

22.

Substrate-induced changes in the structural properties of LacY.

Serdiuk T, Madej MG, Sugihara J, Kawamura S, Mari SA, Kaback HR, Müller DJ.

Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):E1571-80. doi: 10.1073/pnas.1404446111. Epub 2014 Apr 7.

23.

Functional architecture of MFS D-glucose transporters.

Madej MG, Sun L, Yan N, Kaback HR.

Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):E719-27. doi: 10.1073/pnas.1400336111. Epub 2014 Feb 3.

24.

Galactoside-binding site in LacY.

Jiang X, Villafuerte MK, Andersson M, White SH, Kaback HR.

Biochemistry. 2014 Mar 11;53(9):1536-43. doi: 10.1021/bi401716z. Epub 2014 Feb 26.

25.

Structure of sugar-bound LacY.

Kumar H, Kasho V, Smirnova I, Finer-Moore JS, Kaback HR, Stroud RM.

Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1784-8. doi: 10.1073/pnas.1324141111. Epub 2014 Jan 22.

26.

Structure-based mechanism for Na(+)/melibiose symport by MelB.

Ethayathulla AS, Yousef MS, Amin A, Leblanc G, Kaback HR, Guan L.

Nat Commun. 2014;5:3009. doi: 10.1038/ncomms4009.

27.

Evolutionary mix-and-match with MFS transporters II.

Madej MG, Kaback HR.

Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):E4831-8. doi: 10.1073/pnas.1319754110. Epub 2013 Nov 20.

28.

Electrophysiological characterization of uncoupled mutants of LacY.

Gaiko O, Bazzone A, Fendler K, Kaback HR.

Biochemistry. 2013 Nov 19;52(46):8261-6. doi: 10.1021/bi4013269. Epub 2013 Nov 8.

PMID:
24152072
29.

The periplasmic cavity of LacY mutant Cys154→Gly: how open is open?

Jiang X, Driessen AJ, Feringa BL, Kaback HR.

Biochemistry. 2013 Sep 17;52(37):6568-74. doi: 10.1021/bi401026d. Epub 2013 Aug 30.

30.

YidC protein, a molecular chaperone for LacY protein folding via the SecYEG protein machinery.

Zhu L, Kaback HR, Dalbey RE.

J Biol Chem. 2013 Sep 27;288(39):28180-94. doi: 10.1074/jbc.M113.491613. Epub 2013 Aug 8.

31.

Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation.

Smirnova I, Kasho V, Sugihara J, Kaback HR.

Proc Natl Acad Sci U S A. 2013 May 28;110(22):8876-81. doi: 10.1073/pnas.1306849110. Epub 2013 May 13.

32.

Evolutionary mix-and-match with MFS transporters.

Madej MG, Dang S, Yan N, Kaback HR.

Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5870-4. doi: 10.1073/pnas.1303538110. Epub 2013 Mar 25.

33.

Role of protons in sugar binding to LacY.

Smirnova I, Kasho V, Sugihara J, Vázquez-Ibar JL, Kaback HR.

Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16835-40. doi: 10.1073/pnas.1214890109. Epub 2012 Oct 2.

34.

Apo-intermediate in the transport cycle of lactose permease (LacY).

Madej MG, Soro SN, Kaback HR.

Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):E2970-8. doi: 10.1073/pnas.1211183109. Epub 2012 Sep 24.

35.

Proton-coupled dynamics in lactose permease.

Andersson M, Bondar AN, Freites JA, Tobias DJ, Kaback HR, White SH.

Structure. 2012 Nov 7;20(11):1893-904. doi: 10.1016/j.str.2012.08.021. Epub 2012 Sep 20.

36.

Dynamics of the L-fucose/H+ symporter revealed by fluorescence spectroscopy.

Sugihara J, Sun L, Yan N, Kaback HR.

Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):14847-51. doi: 10.1073/pnas.1213445109. Epub 2012 Aug 28.

37.

Role of the irreplaceable residues in the LacY alternating access mechanism.

Zhou Y, Jiang X, Kaback HR.

Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12438-42. doi: 10.1073/pnas.1210684109. Epub 2012 Jul 16.

38.

Evidence for an intermediate conformational state of LacY.

Jiang X, Guan L, Zhou Y, Hong WX, Zhang Q, Kaback HR.

Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E698-704. doi: 10.1073/pnas.1201107109. Epub 2012 Feb 21.

39.

Sugar recognition by CscB and LacY.

Sugihara J, Smirnova I, Kasho V, Kaback HR.

Biochemistry. 2011 Dec 27;50(51):11009-14. doi: 10.1021/bi201592y. Epub 2011 Dec 1.

40.

Lactose permease and the alternating access mechanism.

Smirnova I, Kasho V, Kaback HR.

Biochemistry. 2011 Nov 15;50(45):9684-93. doi: 10.1021/bi2014294. Epub 2011 Oct 19.

41.

Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding.

Smirnova I, Kasho V, Sugihara J, Kaback HR.

Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15147-51. doi: 10.1073/pnas.1112157108. Epub 2011 Sep 6.

42.

An early event in the transport mechanism of LacY protein: interaction between helices V and I.

Zhou Y, Madej MG, Guan L, Nie Y, Kaback HR.

J Biol Chem. 2011 Sep 2;286(35):30415-22. doi: 10.1074/jbc.M111.268433. Epub 2011 Jul 5.

43.

Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.

Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J.

Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. doi: 10.1073/pnas.1105687108. Epub 2011 May 18.

44.

Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport.

Jiang X, Nie Y, Kaback HR.

Biochemistry. 2011 Mar 15;50(10):1634-40. doi: 10.1021/bi101988s. Epub 2011 Feb 8.

45.

The alternating access transport mechanism in LacY.

Kaback HR, Smirnova I, Kasho V, Nie Y, Zhou Y.

J Membr Biol. 2011 Jan;239(1-2):85-93. doi: 10.1007/s00232-010-9327-5. Epub 2010 Dec 16. Review.

46.

Delineating electrogenic reactions during lactose/H+ symport.

Garcia-Celma JJ, Ploch J, Smirnova I, Kaback HR, Fendler K.

Biochemistry. 2010 Jul 27;49(29):6115-21. doi: 10.1021/bi100492p.

47.

Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane.

Nie Y, Kaback HR.

Proc Natl Acad Sci U S A. 2010 May 25;107(21):9903-8. doi: 10.1073/pnas.1004515107. Epub 2010 May 10.

48.

Climate change and the integrity of science.

Gleick PH, Adams RM, Amasino RM, Anders E, Anderson DJ, Anderson WW, Anselin LE, Arroyo MK, Asfaw B, Ayala FJ, Bax A, Bebbington AJ, Bell G, Bennett MV, Bennetzen JL, Berenbaum MR, Berlin OB, Bjorkman PJ, Blackburn E, Blamont JE, Botchan MR, Boyer JS, Boyle EA, Branton D, Briggs SP, Briggs WR, Brill WJ, Britten RJ, Broecker WS, Brown JH, Brown PO, Brunger AT, Cairns J Jr, Canfield DE, Carpenter SR, Carrington JC, Cashmore AR, Castilla JC, Cazenave A, Chapin FS 3rd, Ciechanover AJ, Clapham DE, Clark WC, Clayton RN, Coe MD, Conwell EM, Cowling EB, Cowling RM, Cox CS, Croteau RB, Crothers DM, Crutzen PJ, Daily GC, Dalrymple GB, Dangl JL, Darst SA, Davies DR, Davis MB, De Camilli PV, Dean C, DeFries RS, Deisenhofer J, Delmer DP, DeLong EF, DeRosier DJ, Diener TO, Dirzo R, Dixon JE, Donoghue MJ, Doolittle RF, Dunne T, Ehrlich PR, Eisenstadt SN, Eisner T, Emanuel KA, Englander SW, Ernst WG, Falkowski PG, Feher G, Ferejohn JA, Fersht A, Fischer EH, Fischer R, Flannery KV, Frank J, Frey PA, Fridovich I, Frieden C, Futuyma DJ, Gardner WR, Garrett CJ, Gilbert W, Goldberg RB, Goodenough WH, Goodman CS, Goodman M, Greengard P, Hake S, Hammel G, Hanson S, Harrison SC, Hart SR, Hartl DL, Haselkorn R, Hawkes K, Hayes JM, Hille B, Hökfelt T, House JS, Hout M, Hunten DM, Izquierdo IA, Jagendorf AT, Janzen DH, Jeanloz R, Jencks CS, Jury WA, Kaback HR, Kailath T, Kay P, Kay SA, Kennedy D, Kerr A, Kessler RC, Khush GS, Kieffer SW, Kirch PV, Kirk K, Kivelson MG, Klinman JP, Klug A, Knopoff L, Kornberg H, Kutzbach JE, Lagarias JC, Lambeck K, Landy A, Langmuir CH, Larkins BA, Le Pichon XT, Lenski RE, Leopold EB, Levin SA, Levitt M, Likens GE, Lippincott-Schwartz J, Lorand L, Lovejoy CO, Lynch M, Mabogunje AL, Malone TF, Manabe S, Marcus J, Massey DS, McWilliams JC, Medina E, Melosh HJ, Meltzer DJ, Michener CD, Miles EL, Mooney HA, Moore PB, Morel FM, Mosley-Thompson ES, Moss B, Munk WH, Myers N, Nair GB, Nathans J, Nester EW, Nicoll RA, Novick RP, O'Connell JF, Olsen PE, Opdyke ND, Oster GF, Ostrom E, Pace NR, Paine RT, Palmiter RD, Pedlosky J, Petsko GA, Pettengill GH, Philander SG, Piperno DR, Pollard TD, Price PB Jr, Reichard PA, Reskin BF, Ricklefs RE, Rivest RL, Roberts JD, Romney AK, Rossmann MG, Russell DW, Rutter WJ, Sabloff JA, Sagdeev RZ, Sahlins MD, Salmond A, Sanes JR, Schekman R, Schellnhuber J, Schindler DW, Schmitt J, Schneider SH, Schramm VL, Sederoff RR, Shatz CJ, Sherman F, Sidman RL, Sieh K, Simons EL, Singer BH, Singer MF, Skyrms B, Sleep NH, Smith BD, Snyder SH, Sokal RR, Spencer CS, Steitz TA, Strier KB, Südhof TC, Taylor SS, Terborgh J, Thomas DH, Thompson LG, Tjian RT, Turner MG, Uyeda S, Valentine JW, Valentine JS, Van Etten JL, van Holde KE, Vaughan M, Verba S, von Hippel PH, Wake DB, Walker A, Walker JE, Watson EB, Watson PJ, Weigel D, Wessler SR, West-Eberhard MJ, White TD, Wilson WJ, Wolfenden RV, Wood JA, Woodwell GM, Wright HE Jr, Wu C, Wunsch C, Zoback ML.

Science. 2010 May 7;328(5979):689-90. doi: 10.1126/science.328.5979.689. No abstract available. Erratum in: Science. 2010 May 14;328(5980):826.

49.

Helix dynamics in LacY: helices II and IV.

Liu Z, Madej MG, Kaback HR.

J Mol Biol. 2010 Feb 26;396(3):617-26. doi: 10.1016/j.jmb.2009.12.044. Epub 2010 Jan 4.

50.

Probing of the rates of alternating access in LacY with Trp fluorescence.

Smirnova I, Kasho V, Sugihara J, Kaback HR.

Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21561-6. doi: 10.1073/pnas.0911434106. Epub 2009 Dec 3.

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