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Items: 1 to 50 of 86

1.

Establishing a high-yielding cell-free protein synthesis platform derived from Vibrio natriegens.

Des Soye B, Davidson S, Weinstock M, Gibson D, Jewett MC.

ACS Synth Biol. 2018 Aug 14. doi: 10.1021/acssynbio.8b00252. [Epub ahead of print]

PMID:
30107122
2.

Within-gene Shine-Dalgarno sequences are not selected for function.

Hockenberry AJ, Jewett MC, Amaral LAN, Wilke CO.

Mol Biol Evol. 2018 Aug 1. doi: 10.1093/molbev/msy150. [Epub ahead of print]

PMID:
30085185
3.

BioBits™ Bright: A fluorescent synthetic biology education kit.

Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante TC, Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, Donghia N, Pardee K, Collins JJ, Jewett MC.

Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. eCollection 2018 Aug.

4.

BioBits™ Explorer: A modular synthetic biology education kit.

Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ.

Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug.

5.

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

Jaroentomeechai T, Stark JC, Natarajan A, Glasscock CJ, Yates LE, Hsu KJ, Mrksich M, Jewett MC, DeLisa MP.

Nat Commun. 2018 Jul 12;9(1):2686. doi: 10.1038/s41467-018-05110-x.

6.

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.

Kightlinger W, Lin L, Rosztoczy M, Li W, DeLisa MP, Mrksich M, Jewett MC.

Nat Chem Biol. 2018 May 7. doi: 10.1038/s41589-018-0051-2. [Epub ahead of print]

PMID:
29736039
7.

Engineered Ribosomes for Basic Science and Synthetic Biology.

d'Aquino AE, Kim DS, Jewett MC.

Annu Rev Chem Biomol Eng. 2018 Jun 7;9:311-340. doi: 10.1146/annurev-chembioeng-060817-084129. Epub 2018 Mar 28.

PMID:
29589973
8.

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids.

Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, Majewska NI, Chen CX, Marcum RD, Weiss MG, Stoddart AE, Amiram M, Ranji Charna AK, Patel JR, Isaacs FJ, Kelleher NL, Hong SH, Jewett MC.

Nat Commun. 2018 Mar 23;9(1):1203. doi: 10.1038/s41467-018-03469-5.

9.

A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology.

Casini A, Chang FY, Eluere R, King AM, Young EM, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Borujeni AE, Ryu MH, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O'Connor S, Medema MH, Fischbach MA, Jewett MC, Voigt C, Gordon DB.

J Am Chem Soc. 2018 Mar 28;140(12):4302-4316. doi: 10.1021/jacs.7b13292. Epub 2018 Mar 16.

PMID:
29480720
10.

How many human proteoforms are there?

Aebersold R, Agar JN, Amster IJ, Baker MS, Bertozzi CR, Boja ES, Costello CE, Cravatt BF, Fenselau C, Garcia BA, Ge Y, Gunawardena J, Hendrickson RC, Hergenrother PJ, Huber CG, Ivanov AR, Jensen ON, Jewett MC, Kelleher NL, Kiessling LL, Krogan NJ, Larsen MR, Loo JA, Ogorzalek Loo RR, Lundberg E, MacCoss MJ, Mallick P, Mootha VK, Mrksich M, Muir TW, Patrie SM, Pesavento JJ, Pitteri SJ, Rodriguez H, Saghatelian A, Sandoval W, Schlüter H, Sechi S, Slavoff SA, Smith LM, Snyder MP, Thomas PM, Uhlén M, Van Eyk JE, Vidal M, Walt DR, White FM, Williams ER, Wohlschlager T, Wysocki VH, Yates NA, Young NL, Zhang B.

Nat Chem Biol. 2018 Feb 14;14(3):206-214. doi: 10.1038/nchembio.2576.

PMID:
29443976
11.

Incorporation of Nonproteinogenic Amino Acids in Class I and II Lantibiotics.

Kakkar N, Perez JG, Liu WR, Jewett MC, van der Donk WA.

ACS Chem Biol. 2018 Apr 20;13(4):951-957. doi: 10.1021/acschembio.7b01024. Epub 2018 Feb 21.

PMID:
29439566
12.

A novel framework for evaluating the performance of codon usage bias metrics.

Liu SS, Hockenberry AJ, Jewett MC, Amaral LAN.

J R Soc Interface. 2018 Jan;15(138). pii: 20170667. doi: 10.1098/rsif.2017.0667.

PMID:
29386398
13.

Toward an orthogonal central dogma.

Liu CC, Jewett MC, Chin JW, Voigt CA.

Nat Chem Biol. 2018 Jan 16;14(2):103-106. doi: 10.1038/nchembio.2554. No abstract available.

PMID:
29337969
14.

Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands.

Hockenberry AJ, Stern AJ, Amaral LAN, Jewett MC.

Mol Biol Evol. 2017 Dec 6. doi: 10.1093/molbev/msx310. [Epub ahead of print]

15.

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases.

Schoborg JA, Hershewe JM, Stark JC, Kightlinger W, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP, Jewett MC.

Biotechnol Bioeng. 2018 Mar;115(3):739-750. doi: 10.1002/bit.26502. Epub 2017 Dec 26.

PMID:
29178580
16.

Editorial overview: Synthetic biology: Frontiers in synthetic biology.

Jewett MC, Ellis T.

Curr Opin Chem Biol. 2017 Oct;40:A1-A3. doi: 10.1016/j.cbpa.2017.10.027. No abstract available.

PMID:
29156192
17.

Controlling cell-free metabolism through physiochemical perturbations.

Karim AS, Heggestad JT, Crowe SA, Jewett MC.

Metab Eng. 2018 Jan;45:86-94. doi: 10.1016/j.ymben.2017.11.005. Epub 2017 Nov 15.

PMID:
29155060
18.

A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases.

Jaroentomeechai T, Zheng X, Hershewe J, Stark JC, Jewett MC, DeLisa MP.

Methods Enzymol. 2017;597:55-81. doi: 10.1016/bs.mie.2017.07.011. Epub 2017 Sep 13.

PMID:
28935112
19.

Repurposing ribosomes for synthetic biology.

Liu Y, Kim DS, Jewett MC.

Curr Opin Chem Biol. 2017 Oct;40:87-94. doi: 10.1016/j.cbpa.2017.07.012. Epub 2017 Sep 1. Review.

PMID:
28869851
20.

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies.

Martin RW, Majewska NI, Chen CX, Albanetti TE, Jimenez RBC, Schmelzer AE, Jewett MC, Roy V.

ACS Synth Biol. 2017 Jul 21;6(7):1370-1379. doi: 10.1021/acssynbio.7b00001. Epub 2017 Apr 18.

PMID:
28350472
21.

Cogenerating Synthetic Parts toward a Self-Replicating System.

Li J, Haas W, Jackson K, Kuru E, Jewett MC, Fan ZH, Gygi S, Church GM.

ACS Synth Biol. 2017 Jul 21;6(7):1327-1336. doi: 10.1021/acssynbio.6b00342. Epub 2017 Mar 29.

PMID:
28330337
22.

Establishing a high yielding streptomyces-based cell-free protein synthesis system.

Li J, Wang H, Kwon YC, Jewett MC.

Biotechnol Bioeng. 2017 Jun;114(6):1343-1353. doi: 10.1002/bit.26253. Epub 2017 Feb 23.

PMID:
28112394
23.

Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency.

Hockenberry AJ, Pah AR, Jewett MC, Amaral LA.

Open Biol. 2017 Jan;7(1). pii: 160239. doi: 10.1098/rsob.160239.

24.

Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.

Gan R, Perez JG, Carlson ED, Ntai I, Isaacs FJ, Kelleher NL, Jewett MC.

Biotechnol Bioeng. 2017 May;114(5):1074-1086. doi: 10.1002/bit.26239. Epub 2017 Feb 2.

25.

NullSeq: A Tool for Generating Random Coding Sequences with Desired Amino Acid and GC Contents.

Liu SS, Hockenberry AJ, Lancichinetti A, Jewett MC, Amaral LA.

PLoS Comput Biol. 2016 Nov 11;12(11):e1005184. doi: 10.1371/journal.pcbi.1005184. eCollection 2016 Nov.

26.

Cell-Free Synthetic Biology: Engineering Beyond the Cell.

Perez JG, Stark JC, Jewett MC.

Cold Spring Harb Perspect Biol. 2016 Dec 1;8(12). pii: a023853. doi: 10.1101/cshperspect.a023853. Review.

PMID:
27742731
27.

Depletion of Shine-Dalgarno Sequences within Bacterial Coding Regions Is Expression Dependent.

Yang C, Hockenberry AJ, Jewett MC, Amaral LA.

G3 (Bethesda). 2016 Sep 7. pii: g3.116.032227. doi: 10.1534/g3.116.032227. [Epub ahead of print]

28.

In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis.

Goering AW, Li J, McClure RA, Thomson RJ, Jewett MC, Kelleher NL.

ACS Synth Biol. 2017 Jan 20;6(1):39-44. doi: 10.1021/acssynbio.6b00160. Epub 2016 Aug 9.

29.

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis.

Dudley QM, Anderson KC, Jewett MC.

ACS Synth Biol. 2016 Dec 16;5(12):1578-1588. Epub 2016 Aug 22.

PMID:
27476989
30.

Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis.

Schoborg JA, Clark LG, Choudhury A, Hodgman CE, Jewett MC.

Synth Syst Biotechnol. 2016 Apr 6;1(1):2-6. doi: 10.1016/j.synbio.2016.02.004. eCollection 2016 Mar.

31.

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes.

Caschera F, Lee JW, Ho KK, Liu AP, Jewett MC.

Chem Commun (Camb). 2016 Apr 7;52(31):5467-9. doi: 10.1039/c6cc00223d.

32.

Synthetic biology: Tailor-made genetic codes.

Jewett MC, Noireaux V.

Nat Chem. 2016 Apr;8(4):291-2. doi: 10.1038/nchem.2484. No abstract available.

PMID:
27001721
33.

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery.

Karim AS, Jewett MC.

Metab Eng. 2016 Jul;36:116-126. doi: 10.1016/j.ymben.2016.03.002. Epub 2016 Mar 17.

PMID:
26996382
34.

Editorial: Transforming biotechnology with synthetic biology.

Chen GG, Jewett MC.

Biotechnol J. 2016 Feb;11(2):193-4. doi: 10.1002/biot.201600010.

PMID:
26844671
35.

Evolution of translation initiation sequences using in vitro yeast ribosome display.

Gan R, Jewett MC.

Biotechnol Bioeng. 2016 Aug;113(8):1777-86. doi: 10.1002/bit.25933. Epub 2016 Feb 4.

PMID:
26757179
36.

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids.

Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Söll D, Rinehart J, Isaacs FJ.

Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.

37.

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol.

Kay JE, Jewett MC.

Metab Eng. 2015 Nov;32:133-142. doi: 10.1016/j.ymben.2015.09.015. Epub 2015 Sep 30.

PMID:
26428449
38.

A cell-free expression and purification process for rapid production of protein biologics.

Sullivan CJ, Pendleton ED, Sasmor HH, Hicks WL, Farnum JB, Muto M, Amendt EM, Schoborg JA, Martin RW, Clark LG, Anderson MJ, Choudhury A, Fior R, Lo YH, Griffey RH, Chappell SA, Jewett MC, Mauro VP, Dresios J.

Biotechnol J. 2016 Feb;11(2):238-48. doi: 10.1002/biot.201500214. Epub 2015 Dec 7.

PMID:
26427345
39.

Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase.

Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett MC.

Biotechnol J. 2016 Feb;11(2):212-8. doi: 10.1002/biot.201500030. Epub 2015 Sep 10.

PMID:
26356243
40.

Robust production of recombinant phosphoproteins using cell-free protein synthesis.

Oza JP, Aerni HR, Pirman NL, Barber KW, ter Haar CM, Rogulina S, Amrofell MB, Isaacs FJ, Rinehart J, Jewett MC.

Nat Commun. 2015 Sep 9;6:8168. doi: 10.1038/ncomms9168.

41.

Protein synthesis by ribosomes with tethered subunits.

Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS.

Nature. 2015 Aug 6;524(7563):119-24. doi: 10.1038/nature14862. Epub 2015 Jul 29.

PMID:
26222032
42.

Repurposing the translation apparatus for synthetic biology.

Soye BJD, Patel JR, Isaacs FJ, Jewett MC.

Curr Opin Chem Biol. 2015 Oct;28:83-90. doi: 10.1016/j.cbpa.2015.06.008. Epub 2015 Jul 15. Review.

43.

Correction to: A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe.

Gan R, Jewett MC.

Biotechnol J. 2015 Jul;10(7):1090. doi: 10.1002/biot.201590015. No abstract available.

PMID:
26153360
44.

Self-Assembly of Temperature-Responsive Protein-Polymer Bioconjugates.

Moatsou D, Li J, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O'Reilly RK.

Bioconjug Chem. 2015 Sep 16;26(9):1890-9. doi: 10.1021/acs.bioconjchem.5b00264. Epub 2015 Jul 2.

45.

Energizing eukaryotic cell-free protein synthesis with glucose metabolism.

Anderson MJ, Stark JC, Hodgman CE, Jewett MC.

FEBS Lett. 2015 Jul 8;589(15):1723-1727. doi: 10.1016/j.febslet.2015.05.045. Epub 2015 Jun 6.

46.

Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction.

Fritz BR, Jamil OK, Jewett MC.

Nucleic Acids Res. 2015 May 19;43(9):4774-84. doi: 10.1093/nar/gkv329. Epub 2015 Apr 20.

47.

pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders.

Heinzelman P, Schoborg JA, Jewett MC.

Protein Eng Des Sel. 2015 Oct;28(10):481-9. doi: 10.1093/protein/gzv022. Epub 2015 Apr 15.

48.

Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1.

Hong SH, Kwon YC, Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, Jewett MC.

Chembiochem. 2015 Mar 23;16(5):844-53. doi: 10.1002/cbic.201402708. Epub 2015 Mar 3.

49.

High-throughput preparation methods of crude extract for robust cell-free protein synthesis.

Kwon YC, Jewett MC.

Sci Rep. 2015 Mar 2;5:8663. doi: 10.1038/srep08663.

50.

Controlled assembly of artificial protein-protein complexes via DNA duplex formation.

Płoskoń E, Wagner SC, Ellington AD, Jewett MC, O'Reilly R, Booth PJ.

Bioconjug Chem. 2015 Mar 18;26(3):427-34. doi: 10.1021/bc500473s. Epub 2015 Feb 17.

PMID:
25603321

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