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Biosci Rep. 2015 May 14;35(3). pii: e00212. doi: 10.1042/BSR20150067.

Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?

Author information

1
Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland a_bajek@wp.pl.
2
Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland.
3
Chair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
4
Department of Clinical Pathomorphology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
5
Chair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland Department of Microbiology and Immunology, The Children's Memorial Health Institute, 04-730 Warsaw, Poland.
6
Department of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland Department of Urology, Nicolaus Copernicus Hospital, 87-100 Torun, Poland.

Abstract

Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.

KEYWORDS:

adipose-derived stem cells; liposuction; markers' extensive analysis; phenotypic characterization

PMID:
26182374
PMCID:
PMC4613685
DOI:
10.1042/BSR20150067
[Indexed for MEDLINE]
Free PMC Article

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