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Items: 24


Isomerization of BRCA1-BARD1 promotes replication fork protection.

Daza-Martin M, Starowicz K, Jamshad M, Tye S, Ronson GE, MacKay HL, Chauhan AS, Walker AK, Stone HR, Beesley JFJ, Coles JL, Garvin AJ, Stewart GS, McCorvie TJ, Zhang X, Densham RM, Morris JR.

Nature. 2019 Jul;571(7766):521-527. doi: 10.1038/s41586-019-1363-4. Epub 2019 Jul 3.


The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity.

Jamshad M, Knowles TJ, White SA, Ward DG, Mohammed F, Rahman KF, Wynne M, Hughes GW, Kramer G, Bukau B, Huber D.

Elife. 2019 Jun 27;8. pii: e48385. doi: 10.7554/eLife.48385.


Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system.

Hughes GW, Hall SCL, Laxton CS, Sridhar P, Mahadi AH, Hatton C, Piggot TJ, Wotherspoon PJ, Leney AC, Ward DG, Jamshad M, Spana V, Cadby IT, Harding C, Isom GL, Bryant JA, Parr RJ, Yakub Y, Jeeves M, Huber D, Henderson IR, Clifton LA, Lovering AL, Knowles TJ.

Nat Microbiol. 2019 Oct;4(10):1692-1705. doi: 10.1038/s41564-019-0481-y. Epub 2019 Jun 24.


The deSUMOylase SENP2 coordinates homologous recombination and nonhomologous end joining by independent mechanisms.

Garvin AJ, Walker AK, Densham RM, Chauhan AS, Stone HR, Mackay HL, Jamshad M, Starowicz K, Daza-Martin M, Ronson GE, Lanz AJ, Beesley JF, Morris JR.

Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. Epub 2019 Feb 22.


A new panel of epitope mapped monoclonal antibodies recognising the prototypical tetraspanin CD81.

Grove J, Hu K, Farquhar MJ, Goodall M, Walker L, Jamshad M, Drummer HE, Bill RM, Balfe P, McKeating JA.

Wellcome Open Res. 2017 Sep 7;2:82. doi: 10.12688/wellcomeopenres.12058.1. eCollection 2017.


MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis.

Isom GL, Davies NJ, Chong ZS, Bryant JA, Jamshad M, Sharif M, Cunningham AF, Knowles TJ, Chng SS, Cole JA, Henderson IR.

Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.1038/s41598-017-09111-6.


Structural and Functional Analysis of the Escherichia coli Acid-Sensing Histidine Kinase EvgS.

Sen H, Aggarwal N, Ishionwu C, Hussain N, Parmar C, Jamshad M, Bavro VN, Lund PA.

J Bacteriol. 2017 Aug 22;199(18). pii: e00310-17. doi: 10.1128/JB.00310-17. Print 2017 Sep 15.


SecA Cotranslationally Interacts with Nascent Substrate Proteins In Vivo.

Huber D, Jamshad M, Hanmer R, Schibich D, Döring K, Marcomini I, Kramer G, Bukau B.

J Bacteriol. 2016 Dec 28;199(2). pii: e00622-16. doi: 10.1128/JB.00622-16. Print 2017 Jan 15.


A method for detergent-free isolation of membrane proteins in their local lipid environment.

Lee SC, Knowles TJ, Postis VL, Jamshad M, Parslow RA, Lin YP, Goldman A, Sridhar P, Overduin M, Muench SP, Dafforn TR.

Nat Protoc. 2016 Jul;11(7):1149-62. doi: 10.1038/nprot.2016.070. Epub 2016 Jun 2.


GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential.

Wheatley M, Charlton J, Jamshad M, Routledge SJ, Bailey S, La-Borde PJ, Azam MT, Logan RT, Bill RM, Dafforn TR, Poyner DR.

Biochem Soc Trans. 2016 Apr 15;44(2):619-23. doi: 10.1042/BST20150284. Review.


Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins.

Jamshad M, Grimard V, Idini I, Knowles TJ, Dowle MR, Schofield N, Sridhar P, Lin YP, Finka R, Wheatley M, Thomas OR, Palmer RE, Overduin M, Govaerts C, Ruysschaert JM, Edler KJ, Dafforn TR.

Nano Res. 2015 Mar;8(3):774-789. doi: 10.1007/s12274-014-0560-6. Epub 2014 Oct 23.


G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent.

Jamshad M, Charlton J, Lin YP, Routledge SJ, Bawa Z, Knowles TJ, Overduin M, Dekker N, Dafforn TR, Bill RM, Poyner DR, Wheatley M.

Biosci Rep. 2015 Apr 16;35(2). pii: e00188. doi: 10.1042/BSR20140171.


Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.

Bawa Z, Routledge SJ, Jamshad M, Clare M, Sarkar D, Dickerson I, Ganzlin M, Poyner DR, Bill RM.

Microb Cell Fact. 2014 Sep 4;13(1):127. doi: 10.1186/s12934-014-0127-y.


Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a.

Paulin S, Jamshad M, Dafforn TR, Garcia-Lara J, Foster SJ, Galley NF, Roper DI, Rosado H, Taylor PW.

Nanotechnology. 2014 Jul 18;25(28):285101. doi: 10.1088/0957-4484/25/28/285101. Epub 2014 Jun 27.


Detergent-free purification of ABC (ATP-binding-cassette) transporters.

Gulati S, Jamshad M, Knowles TJ, Morrison KA, Downing R, Cant N, Collins R, Koenderink JB, Ford RC, Overduin M, Kerr ID, Dafforn TR, Rothnie AJ.

Biochem J. 2014 Jul 15;461(2):269-78. doi: 10.1042/BJ20131477.


Production, purification and characterization of recombinant, full-length human claudin-1.

Bonander N, Jamshad M, Oberthür D, Clare M, Barwell J, Hu K, Farquhar MJ, Stamataki Z, Harris HJ, Dierks K, Dafforn TR, Betzel C, McKeating JA, Bill RM.

PLoS One. 2013 May 21;8(5):e64517. doi: 10.1371/journal.pone.0064517. Print 2013.


Disruption of yeast cells to isolate recombinant proteins.

Jamshad M, Darby RA.

Methods Mol Biol. 2012;866:237-46. doi: 10.1007/978-1-61779-770-5_20.


Yeast transformation to generate high-yielding clones.

Jamshad M, Darby RA.

Methods Mol Biol. 2012;866:57-63. doi: 10.1007/978-1-61779-770-5_6.


Surfactant-free purification of membrane proteins with intact native membrane environment.

Jamshad M, Lin YP, Knowles TJ, Parslow RA, Harris C, Wheatley M, Poyner DR, Bill RM, Thomas OR, Overduin M, Dafforn TR.

Biochem Soc Trans. 2011 Jun;39(3):813-8. doi: 10.1042/BST0390813. Review.


Understanding the yeast host cell response to recombinant membrane protein production.

Bawa Z, Bland CE, Bonander N, Bora N, Cartwright SP, Clare M, Conner MT, Darby RA, Dilworth MV, Holmes WJ, Jamshad M, Routledge SJ, Gross SR, Bill RM.

Biochem Soc Trans. 2011 Jun;39(3):719-23. doi: 10.1042/BST0390719. Erratum in: Biochem Soc Trans. 2011 Aug 1;39(4):1113.


Structural characterization of CD81-Claudin-1 hepatitis C virus receptor complexes.

Bonander N, Jamshad M, Hu K, Farquhar MJ, Stamataki Z, Balfe P, McKeating JA, Bill RM.

Biochem Soc Trans. 2011 Apr;39(2):537-40. doi: 10.1042/BST0390537. Review.


Structural characterization of recombinant human CD81 produced in Pichia pastoris.

Jamshad M, Rajesh S, Stamataki Z, McKeating JA, Dafforn T, Overduin M, Bill RM.

Protein Expr Purif. 2008 Feb;57(2):206-16. Epub 2007 Oct 24.


Identification, mutagenesis, and transcriptional analysis of the methanesulfonate transport operon of Methylosulfonomonas methylovora.

Jamshad M, De Marco P, Pacheco CC, Hanczar T, Murrell JC.

Appl Environ Microbiol. 2006 Jan;72(1):276-83.

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