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Items: 1 to 50 of 204

1.

Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida.

Weihmann R, Domröse A, Drepper T, Jaeger KE, Loeschcke A.

Microb Biotechnol. 2019 Jun 4. doi: 10.1111/1751-7915.13402. [Epub ahead of print]

2.

A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies.

Nazarenko VV, Remeeva A, Yudenko A, Kovalev K, Dubenko A, Goncharov IM, Kuzmichev P, Rogachev AV, Buslaev P, Borshchevskiy V, Mishin A, Dhoke GV, Schwaneberg U, Davari MD, Jaeger KE, Krauss U, Gordeliy V, Gushchin I.

Photochem Photobiol Sci. 2019 May 22. doi: 10.1039/c9pp00067d. [Epub ahead of print]

PMID:
31116222
3.

Pseudomonas putida rDNA is a favored site for the expression of biosynthetic genes.

Domröse A, Hage-Hülsmann J, Thies S, Weihmann R, Kruse L, Otto M, Wierckx N, Jaeger KE, Drepper T, Loeschcke A.

Sci Rep. 2019 May 7;9(1):7028. doi: 10.1038/s41598-019-43405-1.

4.

Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species.

Molitor R, Bollinger A, Kubicki S, Loeschcke A, Jaeger KE, Thies S.

Microb Biotechnol. 2019 Apr 23. doi: 10.1111/1751-7915.13418. [Epub ahead of print]

5.

Interaction of carbohydrate-binding modules with poly(ethylene terephthalate).

Weber J, Petrović D, Strodel B, Smits SHJ, Kolkenbrock S, Leggewie C, Jaeger KE.

Appl Microbiol Biotechnol. 2019 Jun;103(12):4801-4812. doi: 10.1007/s00253-019-09760-9. Epub 2019 Apr 16.

6.

Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis.

Kobus S, Perez-Garcia P, Hoeppner A, Holzscheck N, Kovacic F, Streit WR, Jaeger KE, Chow J, Smits SHJ.

Acta Crystallogr F Struct Biol Commun. 2019 Apr 1;75(Pt 4):307-311. doi: 10.1107/S2053230X19002851. Epub 2019 Apr 2.

PMID:
30950832
7.

Pseudomonas aeruginosa esterase PA2949, a bacterial homolog of the human membrane esterase ABHD6: expression, purification and crystallization.

Bleffert F, Granzin J, Gohlke H, Batra-Safferling R, Jaeger KE, Kovacic F.

Acta Crystallogr F Struct Biol Commun. 2019 Apr 1;75(Pt 4):270-277. doi: 10.1107/S2053230X19002152. Epub 2019 Apr 2.

8.

Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity.

Peters L, Weidenfeld I, Klemm U, Loeschcke A, Weihmann R, Jaeger KE, Drepper T, Ntziachristos V, Stiel AC.

Nat Commun. 2019 Mar 13;10(1):1191. doi: 10.1038/s41467-019-09081-5.

9.

Tailoring the properties of (catalytically)-active inclusion bodies.

Jäger VD, Kloss R, Grünberger A, Seide S, Hahn D, Karmainski T, Piqueray M, Embruch J, Longerich S, Mackfeld U, Jaeger KE, Wiechert W, Pohl M, Krauss U.

Microb Cell Fact. 2019 Feb 7;18(1):33. doi: 10.1186/s12934-019-1081-5.

10.

Decoding the ocean's microbiological secrets for marine enzyme biodiscovery.

Ferrer M, Méndez-García C, Bargiela R, Chow J, Alonso S, García-Moyano A, Bjerga GEK, Steen IH, Schwabe T, Blom C, Vester J, Weckbecker A, Shahgaldian P, de Carvalho CCCR, Meskys R, Zanaroli G, Glöckner FO, Fernández-Guerra A, Thambisetty S, de la Calle F, Golyshina OV, Yakimov MM, Jaeger KE, Yakunin AF, Streit WR, McMeel O, Calewaert JB, Tonné N, Golyshin PN; INMARE Consortium.

FEMS Microbiol Lett. 2019 Jan 1;366(1). doi: 10.1093/femsle/fny285.

11.

Directed Evolution Empowered Redesign of Natural Proteins for the Sustainable Production of Chemicals and Pharmaceuticals.

Bornscheuer UT, Hauer B, Jaeger KE, Schwaneberg U.

Angew Chem Int Ed Engl. 2019 Jan 2;58(1):36-40. doi: 10.1002/anie.201812717. Epub 2018 Dec 6. Review.

PMID:
30520553
12.

Bioprospecting Reveals Class III ω-Transaminases Converting Bulky Ketones and Environmentally Relevant Polyamines.

Coscolín C, Katzke N, García-Moyano A, Navarro-Fernández J, Almendral D, Martínez-Martínez M, Bollinger A, Bargiela R, Gertler C, Chernikova TN, Rojo D, Barbas C, Tran H, Golyshina OV, Koch R, Yakimov MM, Bjerga GEK, Golyshin PN, Jaeger KE, Ferrer M.

Appl Environ Microbiol. 2019 Jan 9;85(2). pii: e02404-18. doi: 10.1128/AEM.02404-18. Print 2019 Jan 15.

13.

An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria.

Endres S, Wingen M, Torra J, Ruiz-González R, Polen T, Bosio G, Bitzenhofer NL, Hilgers F, Gensch T, Nonell S, Jaeger KE, Drepper T.

Sci Rep. 2018 Oct 9;8(1):15021. doi: 10.1038/s41598-018-33291-4.

14.

A Synthetic Reaction Cascade Implemented by Colocalization of Two Proteins within Catalytically Active Inclusion Bodies.

Jäger VD, Lamm R, Kloß R, Kaganovitch E, Grünberger A, Pohl M, Büchs J, Jaeger KE, Krauss U.

ACS Synth Biol. 2018 Sep 21;7(9):2282-2295. doi: 10.1021/acssynbio.8b00274. Epub 2018 Aug 20.

PMID:
30053372
15.

Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants.

Hage-Hülsmann J, Grünberger A, Thies S, Santiago-Schübel B, Klein AS, Pietruszka J, Binder D, Hilgers F, Domröse A, Drepper T, Kohlheyer D, Jaeger KE, Loeschcke A.

PLoS One. 2018 Jul 19;13(7):e0200940. doi: 10.1371/journal.pone.0200940. eCollection 2018.

16.

Mechanistic Basis of the Fast Dark Recovery of the Short LOV Protein DsLOV from Dinoroseobacter shibae.

Fettweiss T, Röllen K, Granzin J, Reiners O, Endres S, Drepper T, Willbold D, Jaeger KE, Batra-Safferling R, Krauss U.

Biochemistry. 2018 Aug 14;57(32):4833-4847. doi: 10.1021/acs.biochem.8b00645. Epub 2018 Jul 24.

PMID:
29989797
17.

Disruption of microbial community composition and identification of plant growth promoting microorganisms after exposure of soil to rapeseed-derived glucosinolates.

Siebers M, Rohr T, Ventura M, Schütz V, Thies S, Kovacic F, Jaeger KE, Berg M, Dörmann P, Schulz M.

PLoS One. 2018 Jul 3;13(7):e0200160. doi: 10.1371/journal.pone.0200160. eCollection 2018.

18.

The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena.

Bollinger A, Thies S, Katzke N, Jaeger KE.

Microb Biotechnol. 2018 Jun 25. doi: 10.1111/1751-7915.13288. [Epub ahead of print] Review.

19.

Preparation of Cyclic Prodiginines by Mutasynthesis in Pseudomonas putida KT2440.

Klein AS, Brass HUC, Klebl DP, Classen T, Loeschcke A, Drepper T, Sievers S, Jaeger KE, Pietruszka J.

Chembiochem. 2018 May 2. doi: 10.1002/cbic.201800154. [Epub ahead of print]

PMID:
29719131
20.

Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study.

Zhao J, Frauenkron-Machedjou VJ, Fulton A, Zhu L, Davari MD, Jaeger KE, Schwaneberg U, Bocola M.

Phys Chem Chem Phys. 2018 Apr 4;20(14):9600-9609. doi: 10.1039/c7cp08470f.

PMID:
29578220
21.

Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX.

Domröse A, Weihmann R, Thies S, Jaeger KE, Drepper T, Loeschcke A.

Synth Syst Biotechnol. 2017 Nov 15;2(4):310-319. doi: 10.1016/j.synbio.2017.11.001. eCollection 2017 Dec.

22.

Chloroplast Signaling Gates Thermotolerance in Arabidopsis.

Dickinson PJ, Kumar M, Martinho C, Yoo SJ, Lan H, Artavanis G, Charoensawan V, Schöttler MA, Bock R, Jaeger KE, Wigge PA.

Cell Rep. 2018 Feb 13;22(7):1657-1665. doi: 10.1016/j.celrep.2018.01.054.

23.

Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various Escherichia coli host strains.

Ihling N, Bittner N, Diederichs S, Schelden M, Korona A, Höfler GT, Fulton A, Jaeger KE, Honda K, Ohtake H, Büchs J.

Biotechnol Prog. 2018 Mar;34(2):315-327. doi: 10.1002/btpr.2600. Epub 2018 Jan 17.

PMID:
29314728
24.

Corrigendum: Directionality of substrate translocation of the hemolysin A Type I secretion system.

Lenders MHH, Weidtkamp-Peters S, Kleinschrodt D, Jaeger KE, Smits SHJ, Schmitt L.

Sci Rep. 2018 Jan 8;8:46926. doi: 10.1038/srep46926.

25.

The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis.

Loeschcke A, Dienst D, Wewer V, Hage-Hülsmann J, Dietsch M, Kranz-Finger S, Hüren V, Metzger S, Urlacher VB, Gigolashvili T, Kopriva S, Axmann IM, Drepper T, Jaeger KE.

PLoS One. 2017 Dec 27;12(12):e0189816. doi: 10.1371/journal.pone.0189816. eCollection 2017.

26.

Heterologous production of long-chain rhamnolipids from Burkholderia glumae in Pseudomonas putida-a step forward to tailor-made rhamnolipids.

Wittgens A, Santiago-Schuebel B, Henkel M, Tiso T, Blank LM, Hausmann R, Hofmann D, Wilhelm S, Jaeger KE, Rosenau F.

Appl Microbiol Biotechnol. 2018 Feb;102(3):1229-1239. doi: 10.1007/s00253-017-8702-x. Epub 2017 Dec 20.

PMID:
29264775
27.

Fast automated online xylanase activity assay using HPAEC-PAD.

Cürten C, Anders N, Juchem N, Ihling N, Volkenborn K, Knapp A, Jaeger KE, Büchs J, Spiess AC.

Anal Bioanal Chem. 2018 Jan;410(1):57-69. doi: 10.1007/s00216-017-0712-0. Epub 2017 Nov 28.

PMID:
29184998
28.

Determinants and Prediction of Esterase Substrate Promiscuity Patterns.

Martínez-Martínez M, Coscolín C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernández J, Bollinger A, Thies S, Méndez-García C, Popovic A, Brown G, Chernikova TN, García-Moyano A, Bjerga GEK, Pérez-García P, Hai T, Del Pozo MV, Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Peláez AI, Sánchez J, Buchholz PCF, Pleiss J, Fernández-Guerra A, Glöckner FO, Golyshina OV, Yakimov MM, Savchenko A, Jaeger KE, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M, The Inmare Consortium.

ACS Chem Biol. 2018 Jan 19;13(1):225-234. doi: 10.1021/acschembio.7b00996. Epub 2017 Dec 20.

PMID:
29182315
29.

High-Throughput Screening Assays for Lipolytic Enzymes.

Fulton A, Hayes MR, Schwaneberg U, Pietruszka J, Jaeger KE.

Methods Mol Biol. 2018;1685:209-231. doi: 10.1007/978-1-4939-7366-8_12.

PMID:
29086311
30.

Electron transfer pathways in a light, oxygen, voltage (LOV) protein devoid of the photoactive cysteine.

Kopka B, Magerl K, Savitsky A, Davari MD, Röllen K, Bocola M, Dick B, Schwaneberg U, Jaeger KE, Krauss U.

Sci Rep. 2017 Oct 17;7(1):13346. doi: 10.1038/s41598-017-13420-1.

31.

An A/U-Rich Enhancer Region Is Required for High-Level Protein Secretion through the HlyA Type I Secretion System.

Khosa S, Scholz R, Schwarz C, Trilling M, Hengel H, Jaeger KE, Smits SHJ, Schmitt L.

Appl Environ Microbiol. 2017 Dec 15;84(1). pii: e01163-17. doi: 10.1128/AEM.01163-17. Print 2018 Jan 1.

32.

Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis.

Skoczinski P, Volkenborn K, Fulton A, Bhadauriya A, Nutschel C, Gohlke H, Knapp A, Jaeger KE.

Microb Cell Fact. 2017 Sep 25;16(1):160. doi: 10.1186/s12934-017-0772-z.

33.

Transcriptional Regulation of the Ambient Temperature Response by H2A.Z Nucleosomes and HSF1 Transcription Factors in Arabidopsis.

Cortijo S, Charoensawan V, Brestovitsky A, Buning R, Ravarani C, Rhodes D, van Noort J, Jaeger KE, Wigge PA.

Mol Plant. 2017 Oct 9;10(10):1258-1273. doi: 10.1016/j.molp.2017.08.014. Epub 2017 Sep 8.

34.

40 Years of Biotechnology Research at Forschungszentrum Jülich.

Bott M, Jaeger KE, Pietruszka J, Wiechert W.

J Biotechnol. 2017 Sep 20;258:1. doi: 10.1016/j.jbiotec.2017.08.014. No abstract available.

PMID:
28890231
35.

The G-Box Transcriptional Regulatory Code in Arabidopsis.

Ezer D, Shepherd SJK, Brestovitsky A, Dickinson P, Cortijo S, Charoensawan V, Box MS, Biswas S, Jaeger KE, Wigge PA.

Plant Physiol. 2017 Oct;175(2):628-640. doi: 10.1104/pp.17.01086. Epub 2017 Sep 1.

36.

First Insights into the Genome Sequence of Pseudomonas oleovorans DSM 1045.

Poehlein A, Daniel R, Thürmer A, Bollinger A, Thies S, Katzke N, Jaeger KE.

Genome Announc. 2017 Aug 10;5(32). pii: e00774-17. doi: 10.1128/genomeA.00774-17.

37.

The evening complex coordinates environmental and endogenous signals in Arabidopsis.

Ezer D, Jung JH, Lan H, Biswas S, Gregoire L, Box MS, Charoensawan V, Cortijo S, Lai X, Stöckle D, Zubieta C, Jaeger KE, Wigge PA.

Nat Plants. 2017 Jun 26;3:17087. doi: 10.1038/nplants.2017.87.

38.

Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.

Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A.

Metab Eng. 2017 Jul;42:145-156. doi: 10.1016/j.ymben.2017.06.009. Epub 2017 Jun 20. Review.

PMID:
28645641
39.

Activity-independent screening of secreted proteins using split GFP.

Knapp A, Ripphahn M, Volkenborn K, Skoczinski P, Jaeger KE.

J Biotechnol. 2017 Sep 20;258:110-116. doi: 10.1016/j.jbiotec.2017.05.024. Epub 2017 Jun 12.

40.

New Prodigiosin Derivatives Obtained by Mutasynthesis in Pseudomonas putida.

Klein AS, Domröse A, Bongen P, Brass HUC, Classen T, Loeschcke A, Drepper T, Laraia L, Sievers S, Jaeger KE, Pietruszka J.

ACS Synth Biol. 2017 Sep 15;6(9):1757-1765. doi: 10.1021/acssynbio.7b00099. Epub 2017 May 26.

PMID:
28505410
41.

A novel FbFP-based biosensor toolbox for sensitive in vivo determination of intracellular pH.

Rupprecht C, Wingen M, Potzkei J, Gensch T, Jaeger KE, Drepper T.

J Biotechnol. 2017 Sep 20;258:25-32. doi: 10.1016/j.jbiotec.2017.05.006. Epub 2017 May 10.

PMID:
28501596
42.

Novel Thermostable Flavin-binding Fluorescent Proteins from Thermophilic Organisms.

Wingen M, Jaeger KE, Gensch T, Drepper T.

Photochem Photobiol. 2017 May;93(3):849-856. doi: 10.1111/php.12740.

PMID:
28500719
43.

Catalytically-active inclusion bodies-Carrier-free protein immobilizates for application in biotechnology and biomedicine.

Krauss U, Jäger VD, Diener M, Pohl M, Jaeger KE.

J Biotechnol. 2017 Sep 20;258:136-147. doi: 10.1016/j.jbiotec.2017.04.033. Epub 2017 Apr 30.

PMID:
28465211
44.

A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase.

Kaschner M, Schillinger O, Fettweiss T, Nutschel C, Krause F, Fulton A, Strodel B, Stadler A, Jaeger KE, Krauss U.

Sci Rep. 2017 Feb 20;7:42592. doi: 10.1038/srep42592.

45.

Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools.

Arinkin V, Granzin J, Röllen K, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R.

Sci Rep. 2017 Feb 17;7:42971. doi: 10.1038/srep42971.

46.

Novel Tools for the Functional Expression of Metagenomic DNA.

Katzke N, Knapp A, Loeschcke A, Drepper T, Jaeger KE.

Methods Mol Biol. 2017;1539:159-196.

PMID:
27900689
47.

Phytochromes function as thermosensors in Arabidopsis.

Jung JH, Domijan M, Klose C, Biswas S, Ezer D, Gao M, Khattak AK, Box MS, Charoensawan V, Cortijo S, Kumar M, Grant A, Locke JC, Schäfer E, Jaeger KE, Wigge PA.

Science. 2016 Nov 18;354(6314):886-889. Epub 2016 Oct 27.

PMID:
27789797
48.

Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.

Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T.

PLoS One. 2016 Aug 15;11(8):e0160711. doi: 10.1371/journal.pone.0160711. eCollection 2016.

49.

Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.

Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T.

Appl Environ Microbiol. 2016 Sep 30;82(20):6141-6149. Print 2016 Oct 15.

50.

A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli.

Kovacic F, Bleffert F, Caliskan M, Wilhelm S, Granzin J, Batra-Safferling R, Jaeger KE.

FEBS Open Bio. 2016 Apr 19;6(5):484-93. doi: 10.1002/2211-5463.12061. eCollection 2016 May.

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