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Screening by changes in stereotypical behavior during cell motility.

Tweedy L, Witzel P, Heinrich D, Insall RH, Endres RG.

Sci Rep. 2019 Jun 19;9(1):8784. doi: 10.1038/s41598-019-45305-w.


Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria.

Paschke P, Knecht DA, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM.

J Vis Exp. 2019 Jan 25;(143). doi: 10.3791/58981.


PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection.

Buckley CM, Heath VL, Guého A, Bosmani C, Knobloch P, Sikakana P, Personnic N, Dove SK, Michell RH, Meier R, Hilbi H, Soldati T, Insall RH, King JS.

PLoS Pathog. 2019 Feb 7;15(2):e1007551. doi: 10.1371/journal.ppat.1007551. eCollection 2019 Feb.


Distinct Interaction Sites of Rac GTPase with WAVE Regulatory Complex Have Non-redundant Functions in Vivo.

Schaks M, Singh SP, Kage F, Thomason P, Klünemann T, Steffen A, Blankenfeldt W, Stradal TE, Insall RH, Rottner K.

Curr Biol. 2018 Nov 19;28(22):3674-3684.e6. doi: 10.1016/j.cub.2018.10.002. Epub 2018 Nov 1.


Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.

Fort L, Batista JM, Thomason PA, Spence HJ, Whitelaw JA, Tweedy L, Greaves J, Martin KJ, Anderson KI, Brown P, Lilla S, Neilson MP, Tafelmeyer P, Zanivan S, Ismail S, Bryant DM, Tomkinson NCO, Chamberlain LH, Mastick GS, Insall RH, Machesky LM.

Nat Cell Biol. 2018 Oct;20(10):1159-1171. doi: 10.1038/s41556-018-0198-9. Epub 2018 Sep 24.


Expression of N471D strumpellin leads to defects in the endolysosomal system.

Song L, Rijal R, Karow M, Stumpf M, Hahn O, Park L, Insall R, Schröder R, Hofmann A, Clemen CS, Eichinger L.

Dis Model Mech. 2018 Sep 13;11(9). pii: dmm033449. doi: 10.1242/dmm.033449.


Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.

Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM.

PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018.


The trimeric coiled-coil HSBP1 protein promotes WASH complex assembly at centrosomes.

Visweshwaran SP, Thomason PA, Guerois R, Vacher S, Denisov EV, Tashireva LA, Lomakina ME, Lazennec-Schurdevin C, Lakisic G, Lilla S, Molinie N, Henriot V, Mechulam Y, Alexandrova AY, Cherdyntseva NV, Bièche I, Schmitt E, Insall RH, Gautreau A.

EMBO J. 2018 Jul 2;37(13). pii: e97706. doi: 10.15252/embj.201797706. Epub 2018 May 29.


A G-protein-coupled chemoattractant receptor recognizes lipopolysaccharide for bacterial phagocytosis.

Pan M, Neilson MP, Grunfeld AM, Cruz P, Wen X, Insall RH, Jin T.

PLoS Biol. 2018 May 25;16(5):e2005754. doi: 10.1371/journal.pbio.2005754. eCollection 2018 May.


The Chemoattractant Glorin Is Inactivated by Ester Cleavage during Early Multicellular Development of Polysphondylium pallidum.

Heinrich D, Barnett R, Tweedy L, Insall R, Stallforth P, Winckler T.

ACS Chem Biol. 2018 Jun 15;13(6):1506-1513. doi: 10.1021/acschembio.8b00046. Epub 2018 May 24.


WASP family proteins and formins compete in pseudopod- and bleb-based migration.

Davidson AJ, Amato C, Thomason PA, Insall RH.

J Cell Biol. 2018 Feb 5;217(2):701-714. doi: 10.1083/jcb.201705160. Epub 2017 Nov 30.


LPP3 mediates self-generation of chemotactic LPA gradients by melanoma cells.

Susanto O, Koh YWH, Morrice N, Tumanov S, Thomason PA, Nielson M, Tweedy L, Muinonen-Martin AJ, Kamphorst JJ, Mackay GM, Insall RH.

J Cell Sci. 2017 Oct 15;130(20):3455-3466. doi: 10.1242/jcs.207514. Epub 2017 Sep 4.


Mroh1, a lysosomal regulator localized by WASH-generated actin.

Thomason PA, King JS, Insall RH.

J Cell Sci. 2017 May 15;130(10):1785-1795. doi: 10.1242/jcs.197210. Epub 2017 Apr 19.


Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation.

Periz J, Whitelaw J, Harding C, Gras S, Del Rosario Minina MI, Latorre-Barragan F, Lemgruber L, Reimer MA, Insall R, Heaslip A, Meissner M.

Elife. 2017 Mar 21;6. pii: e24119. doi: 10.7554/eLife.24119.


Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin.

Woodham EF, Paul NR, Tyrrell B, Spence HJ, Swaminathan K, Scribner MR, Giampazolias E, Hedley A, Clark W, Kage F, Marston DJ, Hahn KM, Tait SW, Larue L, Brakebusch CH, Insall RH, Machesky LM.

Curr Biol. 2017 Mar 6;27(5):624-637. doi: 10.1016/j.cub.2017.01.033. Epub 2017 Feb 23.


A plasma membrane template for macropinocytic cups.

Veltman DM, Williams TD, Bloomfield G, Chen BC, Betzig E, Insall RH, Kay RR.

Elife. 2016 Dec 13;5. pii: e20085. doi: 10.7554/eLife.20085.


Inference of the drivers of collective movement in two cell types: Dictyostelium and melanoma.

Ferguson EA, Matthiopoulos J, Insall RH, Husmeier D.

J R Soc Interface. 2016 Oct;13(123). pii: 20160695.


WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors.

Buckley CM, Gopaldass N, Bosmani C, Johnston SA, Soldati T, Insall RH, King JS.

Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5906-E5915. Epub 2016 Sep 19.


Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.

Tyrrell BJ, Woodham EF, Spence HJ, Strathdee D, Insall RH, Machesky LM.

Pigment Cell Melanoma Res. 2016 Sep;29(5):559-71. doi: 10.1111/pcmr.12506.


Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D.

Susanto O, Muinonen-Martin AJ, Nobis M, Insall RH.

Methods Mol Biol. 2016;1407:217-28. doi: 10.1007/978-1-4939-3480-5_17.


Self-generated chemotactic gradients-cells steering themselves.

Tweedy L, Susanto O, Insall RH.

Curr Opin Cell Biol. 2016 Oct;42:46-51. doi: 10.1016/ Epub 2016 Apr 19. Review.


Self-Generated Chemoattractant Gradients: Attractant Depletion Extends the Range and Robustness of Chemotaxis.

Tweedy L, Knecht DA, Mackay GM, Insall RH.

PLoS Biol. 2016 Mar 16;14(3):e1002404. doi: 10.1371/journal.pbio.1002404. eCollection 2016 Mar.


The Inositol-3-Phosphate Synthase Biosynthetic Enzyme Has Distinct Catalytic and Metabolic Roles.

Frej AD, Clark J, Le Roy CI, Lilla S, Thomason PA, Otto GP, Churchill G, Insall RH, Claus SP, Hawkins P, Stephens L, Williams RS.

Mol Cell Biol. 2016 May 2;36(10):1464-79. doi: 10.1128/MCB.00039-16. Print 2016 May 15.


Overflow in science and its implications for trust.

Siebert S, Machesky LM, Insall RH.

Elife. 2015 Sep 14;4. doi: 10.7554/eLife.10825.


Melanomas' fatal attraction to lysophosphatidic acid trails: a new prognostic and therapeutic approach?

Muinonen-Martin AJ, Insall RH.

Melanoma Manag. 2015 May;2(2):97-100. doi: 10.2217/mmt.15.11. Epub 2015 May 18. No abstract available.


Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation.

Urushihara H, Kuwayama H, Fukuhara K, Itoh T, Kagoshima H, Shin-I T, Toyoda A, Ohishi K, Taniguchi T, Noguchi H, Kuroki Y, Hata T, Uchi K, Mohri K, King JS, Insall RH, Kohara Y, Fujiyama A.

BMC Genomics. 2015 Feb 14;16:80. doi: 10.1186/s12864-015-1278-x.


Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients.

Roberts HM, Ling MR, Insall R, Kalna G, Spengler J, Grant MM, Chapple IL.

J Clin Periodontol. 2015 Jan;42(1):1-11. doi: 10.1111/jcpe.12326. Epub 2015 Jan 8.


Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal.

Muinonen-Martin AJ, Susanto O, Zhang Q, Smethurst E, Faller WJ, Veltman DM, Kalna G, Lindsay C, Bennett DC, Sansom OJ, Herd R, Jones R, Machesky LM, Wakelam MJ, Knecht DA, Insall RH.

PLoS Biol. 2014 Oct 14;12(10):e1001966. doi: 10.1371/journal.pbio.1001966. eCollection 2014 Oct.


SCAR/WAVE: A complex issue.

Davidson AJ, Insall RH.

Commun Integr Biol. 2013 Nov 1;6(6):e27033. doi: 10.4161/cib.27033. Epub 2013 Nov 13.


PIP₃-dependent macropinocytosis is incompatible with chemotaxis.

Veltman DM, Lemieux MG, Knecht DA, Insall RH.

J Cell Biol. 2014 Feb 17;204(4):497-505. doi: 10.1083/jcb.201309081.


Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence.

Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall RH, Stockley RA, Lord JM.

Blood. 2014 Jan 9;123(2):239-48. doi: 10.1182/blood-2013-08-519520. Epub 2013 Nov 4.


Predicting cell shapes in melanomas.

Tyrrell BJ, Neilson M, Insall RH, Machesky LM.

Pigment Cell Melanoma Res. 2014 Jan;27(1):5-6. doi: 10.1111/pcmr.12176. Epub 2013 Oct 23. No abstract available.


Abi is required for modulation and stability but not localization or activation of the SCAR/WAVE complex.

Davidson AJ, Ura S, Thomason PA, Kalna G, Insall RH.

Eukaryot Cell. 2013 Nov;12(11):1509-16. doi: 10.1128/EC.00116-13. Epub 2013 Sep 13.


p21-Activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils.

Itakura A, Aslan JE, Kusanto BT, Phillips KG, Porter JE, Newton PK, Nan X, Insall RH, Chernoff J, McCarty OJ.

PLoS One. 2013 Sep 3;8(9):e73063. doi: 10.1371/journal.pone.0073063. eCollection 2013.


WASH complex conservation: don't WASH away the family!

Insall RH.

Trends Cell Biol. 2013 Nov;23(11):519-20. doi: 10.1016/j.tcb.2013.07.009. Epub 2013 Aug 28. No abstract available.


WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion.

King JS, Gueho A, Hagedorn M, Gopaldass N, Leuba F, Soldati T, Insall RH.

Mol Biol Cell. 2013 Sep;24(17):2714-26. doi: 10.1091/mbc.E13-02-0092. Epub 2013 Jul 24.


Measuring chemotaxis using direct visualization microscope chambers.

Muinonen-Martin AJ, Knecht DA, Veltman DM, Thomason PA, Kalna G, Insall RH.

Methods Mol Biol. 2013;1046:307-21. doi: 10.1007/978-1-62703-538-5_18.


Laparoscopic total extra-peritoneal inguinal hernia repairs by a surgeon experienced at laparoscopic cholecystectomy.

Shirazi MH, Marsh P, Keane MG, Khushal A, Insall R.

J Pak Med Assoc. 2012 Dec;62(12):1301-4.


The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum.

Davidson AJ, King JS, Insall RH.

Biotechniques. 2013 Jul;55(1):39-41. doi: 10.2144/000114054.


The interaction between pseudopods and extracellular signalling during chemotaxis and directed migration.

Insall R.

Curr Opin Cell Biol. 2013 Oct;25(5):526-31. doi: 10.1016/ Epub 2013 Jun 7. Review.


Cyclical action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment.

Park L, Thomason PA, Zech T, King JS, Veltman DM, Carnell M, Ura S, Machesky LM, Insall RH.

Dev Cell. 2013 Jan 28;24(2):169-81. doi: 10.1016/j.devcel.2012.12.014.


Loss of Scar/WAVE complex promotes N-WASP- and FAK-dependent invasion.

Tang H, Li A, Bi J, Veltman DM, Zech T, Spence HJ, Yu X, Timpson P, Insall RH, Frame MC, Machesky LM.

Curr Biol. 2013 Jan 21;23(2):107-17. doi: 10.1016/j.cub.2012.11.059. Epub 2012 Dec 27.


Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium.

Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL.

J Biol Chem. 2013 Jan 25;288(4):2464-74. doi: 10.1074/jbc.M112.435313. Epub 2012 Dec 5.


N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods.

Yu X, Zech T, McDonald L, Gonzalez EG, Li A, Macpherson I, Schwarz JP, Spence H, Futó K, Timpson P, Nixon C, Ma Y, Anton IM, Visegrády B, Insall RH, Oien K, Blyth K, Norman JC, Machesky LM.

J Cell Biol. 2012 Oct 29;199(3):527-44. doi: 10.1083/jcb.201203025. Epub 2012 Oct 22.


Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions.

Spence HJ, Timpson P, Tang HR, Insall RH, Machesky LM.

Biochem J. 2012 Nov 15;448(1):35-42. doi: 10.1042/BJ20112206.


SCAR knockouts in Dictyostelium: WASP assumes SCAR's position and upstream regulators in pseudopods.

Veltman DM, King JS, Machesky LM, Insall RH.

J Cell Biol. 2012 Aug 20;198(4):501-8. doi: 10.1083/jcb.201205058. Epub 2012 Aug 13.


Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation.

Ura S, Pollitt AY, Veltman DM, Morrice NA, Machesky LM, Insall RH.

Curr Biol. 2012 Apr 10;22(7):553-61. doi: 10.1016/j.cub.2012.02.020. Epub 2012 Mar 1.


The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium.

Pakes NK, Veltman DM, Rivero F, Nasir J, Insall R, Williams RS.

J Cell Sci. 2012 May 15;125(Pt 10):2457-65. doi: 10.1242/jcs.100966. Epub 2012 Feb 24.

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