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Items: 41


Escherichia coli "TatExpress" strains export several g/L human growth hormone to the periplasm by the Tat pathway.

Guerrero Montero I, Richards KL, Jawara C, Browning DF, Peswani AR, Labrit M, Allen M, Aubry C, Davé E, Humphreys DP, Busby SJW, Robinson C.

Biotechnol Bioeng. 2019 Dec;116(12):3282-3291. doi: 10.1002/bit.27147. Epub 2019 Sep 2.


Ongoing hybridization obscures phylogenetic relationships in the Drosophila subquinaria species complex.

Ginsberg PS, Humphreys DP, Dyer KA.

J Evol Biol. 2019 Oct;32(10):1093-1105. doi: 10.1111/jeb.13512. Epub 2019 Aug 22.


A robust fractionation method for protein subcellular localization studies in Escherichia coli.

Malherbe G, Humphreys DP, Davé E.

Biotechniques. 2019 Apr;66(4):171-178. doi: 10.2144/btn-2018-0135.


Impact of Human FcγR Gene Polymorphisms on IgG-Triggered Cytokine Release: Critical Importance of Cell Assay Format.

Hussain K, Hargreaves CE, Rowley TF, Sopp JM, Latham KV, Bhatta P, Sherington J, Cutler RM, Humphreys DP, Glennie MJ, Strefford JC, Cragg MS.

Front Immunol. 2019 Mar 7;10:390. doi: 10.3389/fimmu.2019.00390. eCollection 2019.


Mitochondrial aconitase is a key regulator of energy production for growth and protein expression in Chinese hamster ovary cells.

Dhami N, Trivedi DK, Goodacre R, Mainwaring D, Humphreys DP.

Metabolomics. 2018 Oct 1;14(10):136. doi: 10.1007/s11306-018-1430-0.


Fast Estimation of Recombination Rates Using Topological Data Analysis.

Humphreys DP, McGuirl MR, Miyagi M, Blumberg AJ.

Genetics. 2019 Apr;211(4):1191-1204. doi: 10.1534/genetics.118.301565. Epub 2019 Feb 20.


Engineered hexavalent Fc proteins with enhanced Fc-gamma receptor avidity provide insights into immune-complex interactions.

Rowley TF, Peters SJ, Aylott M, Griffin R, Davies NL, Healy LJ, Cutler RM, Eddleston A, Pither TL, Sopp JM, Zaccheo O, Fossati G, Cain K, Ventom AM, Hailu H, Ward EJ, Sherington J, Brennan FR, Fallah-Arani F, Humphreys DP.

Commun Biol. 2018 Sep 14;1:146. doi: 10.1038/s42003-018-0149-9. eCollection 2018.


Fine-scale geographic patterns of gene flow and reproductive character displacement in Drosophila subquinaria and Drosophila recens.

Dyer KA, Bewick ER, White BE, Bray MJ, Humphreys DP.

Mol Ecol. 2018 Aug 3. doi: 10.1111/mec.14825. [Epub ahead of print]


Comparative genomic investigation of high-elevation adaptation in ectothermic snakes.

Li JT, Gao YD, Xie L, Deng C, Shi P, Guan ML, Huang S, Ren JL, Wu DD, Ding L, Huang ZY, Nie H, Humphreys DP, Hillis DM, Wang WZ, Zhang YP.

Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):8406-8411. doi: 10.1073/pnas.1805348115. Epub 2018 Jul 31.


Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.

Qureshi OS, Rowley TF, Junker F, Peters SJ, Crilly S, Compson J, Eddleston A, Björkelund H, Greenslade K, Parkinson M, Davies NL, Griffin R, Pither TL, Cain K, Christodoulou L, Staelens L, Ward E, Tibbitts J, Kiessling A, Smith B, Brennan FR, Malmqvist M, Fallah-Arani F, Humphreys DP.

Sci Rep. 2017 Dec 6;7(1):17049. doi: 10.1038/s41598-017-17255-8.


IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design.

Stoyle CL, Stephens PE, Humphreys DP, Heywood S, Cain K, Bulleid NJ.

Biochem J. 2017 Sep 7;474(18):3179-3188. doi: 10.1042/BCJ20170342.


High prevalence of subclass-specific binding and neutralizing antibodies against Clostridium difficile toxins in adult cystic fibrosis sera: possible mode of immunoprotection against symptomatic C. difficile infection.

Monaghan TM, Negm OH, MacKenzie B, Hamed MR, Shone CC, Humphreys DP, Acharya KR, Wilcox MH.

Clin Exp Gastroenterol. 2017 Jul 19;10:169-175. doi: 10.2147/CEG.S133939. eCollection 2017.


Protective antibodies against Clostridium difficile are present in intravenous immunoglobulin and are retained in humans following its administration.

Negm OH, MacKenzie B, Hamed MR, Ahmad OAJ, Shone CC, Humphreys DP, Ravi Acharya K, Loscher CE, Marszalowska I, Lynch M, Wilcox MH, Monaghan TM.

Clin Exp Immunol. 2017 Jun;188(3):437-443. doi: 10.1111/cei.12946. Epub 2017 Mar 16.


Development of a high yielding E. coli periplasmic expression system for the production of humanized Fab' fragments.

Ellis M, Patel P, Edon M, Ramage W, Dickinson R, Humphreys DP.

Biotechnol Prog. 2017 Jan;33(1):212-220. doi: 10.1002/btpr.2393. Epub 2016 Nov 17.


Fab-dsFv: A bispecific antibody format with extended serum half-life through albumin binding.

Davé E, Adams R, Zaccheo O, Carrington B, Compson JE, Dugdale S, Airey M, Malcolm S, Hailu H, Wild G, Turner A, Heads J, Sarkar K, Ventom A, Marshall D, Jairaj M, Kopotsha T, Christodoulou L, Zamacona M, Lawson AD, Heywood S, Humphreys DP.

MAbs. 2016 Oct;8(7):1319-1335. Epub 2016 Aug 17.


Extending the half-life of a fab fragment through generation of a humanized anti-human serum albumin Fv domain: An investigation into the correlation between affinity and serum half-life.

Adams R, Griffin L, Compson JE, Jairaj M, Baker T, Ceska T, West S, Zaccheo O, Davé E, Lawson AD, Humphreys DP, Heywood S.

MAbs. 2016 Oct;8(7):1336-1346. Epub 2016 Jun 17.


Patterns of reproductive isolation in the Drosophila subquinaria complex: can reinforced premating isolation cascade to other species?

Humphreys DP, Rundle HD, Dyer KA.

Curr Zool. 2016 Apr;62(2):183-191. doi: 10.1093/cz/zow005. Epub 2016 Mar 3.


Phylogenetic structural equation modelling reveals no need for an 'origin' of the leaf economics spectrum.

Mason CM, Goolsby EW, Humphreys DP, Donovan LA.

Ecol Lett. 2016 Jan;19(1):54-61. doi: 10.1111/ele.12542. Epub 2015 Nov 13.


Molecular Insights into the Thermal Stability of mAbs with Variable-Temperature Ion-Mobility Mass Spectrometry.

Pacholarz KJ, Peters SJ, Garlish RA, Henry AJ, Taylor RJ, Humphreys DP, Barran PE.

Chembiochem. 2016 Jan 1;17(1):46-51. doi: 10.1002/cbic.201500574. Epub 2015 Dec 3.


Antibodies for treatment of Clostridium difficile infection.

Humphreys DP, Wilcox MH.

Clin Vaccine Immunol. 2014 Jul;21(7):913-23. doi: 10.1128/CVI.00116-14. Epub 2014 Apr 30. Review.


A mixture of functionally oligoclonal humanized monoclonal antibodies that neutralize Clostridium difficile TcdA and TcdB with high levels of in vitro potency shows in vivo protection in a hamster infection model.

Davies NL, Compson JE, Mackenzie B, O'Dowd VL, Oxbrow AK, Heads JT, Turner A, Sarkar K, Dugdale SL, Jairaj M, Christodoulou L, Knight DE, Cross AS, Hervé KJ, Tyson KL, Hailu H, Doyle CB, Ellis M, Kriek M, Cox M, Page MJ, Moore AR, Lightwood DJ, Humphreys DP.

Clin Vaccine Immunol. 2013 Mar;20(3):377-90. doi: 10.1128/CVI.00625-12. Epub 2013 Jan 16.


Relative stabilities of IgG1 and IgG4 Fab domains: influence of the light-heavy interchain disulfide bond architecture.

Heads JT, Adams R, D'Hooghe LE, Page MJ, Humphreys DP, Popplewell AG, Lawson AD, Henry AJ.

Protein Sci. 2012 Sep;21(9):1315-22. doi: 10.1002/pro.2118. Epub 2012 Aug 9.


Engineering an improved IgG4 molecule with reduced disulfide bond heterogeneity and increased Fab domain thermal stability.

Peters SJ, Smales CM, Henry AJ, Stephens PE, West S, Humphreys DP.

J Biol Chem. 2012 Jul 13;287(29):24525-33. doi: 10.1074/jbc.M112.369744. Epub 2012 May 18.


Towards a universal disulphide stabilised single chain Fv format: importance of interchain disulphide bond location and vL-vH orientation.

Weatherill EE, Cain KL, Heywood SP, Compson JE, Heads JT, Adams R, Humphreys DP.

Protein Eng Des Sel. 2012 Jul;25(7):321-9. doi: 10.1093/protein/gzs021. Epub 2012 May 14.


A processed noncoding RNA regulates an altruistic bacterial antiviral system.

Blower TR, Pei XY, Short FL, Fineran PC, Humphreys DP, Luisi BF, Salmond GP.

Nat Struct Mol Biol. 2011 Feb;18(2):185-90. doi: 10.1038/nsmb.1981. Epub 2011 Jan 16.


Mutagenesis and functional characterization of the RNA and protein components of the toxIN abortive infection and toxin-antitoxin locus of Erwinia.

Blower TR, Fineran PC, Johnson MJ, Toth IK, Humphreys DP, Salmond GP.

J Bacteriol. 2009 Oct;191(19):6029-39. doi: 10.1128/JB.00720-09. Epub 2009 Jul 24.


The phage abortive infection system, ToxIN, functions as a protein-RNA toxin-antitoxin pair.

Fineran PC, Blower TR, Foulds IJ, Humphreys DP, Lilley KS, Salmond GP.

Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):894-9. doi: 10.1073/pnas.0808832106. Epub 2009 Jan 5.


Alternative antibody Fab' fragment PEGylation strategies: combination of strong reducing agents, disruption of the interchain disulphide bond and disulphide engineering.

Humphreys DP, Heywood SP, Henry A, Ait-Lhadj L, Antoniw P, Palframan R, Greenslade KJ, Carrington B, Reeks DG, Bowering LC, West S, Brand HA.

Protein Eng Des Sel. 2007 May;20(5):227-34. Epub 2007 Apr 23.


Engineering of Escherichia coli to improve the purification of periplasmic Fab' fragments: changing the pI of the chromosomally encoded PhoS/PstS protein.

Humphreys DP, Heywood SP, King LM, Bowering LC, Turner JP, Lane SE.

Protein Expr Purif. 2004 Sep;37(1):109-18.


A plasmid system for optimization of Fab' production in Escherichia coli: importance of balance of heavy chain and light chain synthesis.

Humphreys DP, Carrington B, Bowering LC, Ganesh R, Sehdev M, Smith BJ, King LM, Reeks DG, Lawson A, Popplewell AG.

Protein Expr Purif. 2002 Nov;26(2):309-20.


Therapeutic antibody production technologies: molecules, applications, expression and purification.

Humphreys DP, Glover DJ.

Curr Opin Drug Discov Devel. 2001 Mar;4(2):172-85. Review.


High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5' end of the coding sequence.

Humphreys DP, Sehdev M, Chapman AP, Ganesh R, Smith BJ, King LM, Glover DJ, Reeks DG, Stephens PE.

Protein Expr Purif. 2000 Nov;20(2):252-64.


Improved efficiency of site-specific copper(II) ion-catalysed protein cleavage effected by mutagenesis of cleavage site.

Humphreys DP, King LM, West SM, Chapman AP, Sehdev M, Redden MW, Glover DJ, Smith BJ, Stephens PE.

Protein Eng. 2000 Mar;13(3):201-6.


Mutations in dsbA and dsbB, but not dsbC, lead to an enhanced sensitivity of Escherichia coli to Hg2+ and Cd2+.

Stafford SJ, Humphreys DP, Lund PA.

FEMS Microbiol Lett. 1999 May 1;174(1):179-84.


Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage.

Humphreys DP, Smith BJ, King LM, West SM, Reeks DG, Stephens PE.

Protein Eng. 1999 Feb;12(2):179-84.


F(ab')2 molecules made from Escherichia coli produced Fab' with hinge sequences conferring increased serum survival in an animal model.

Humphreys DP, Vetterlein OM, Chapman AP, King DJ, Antoniw P, Suitters AJ, Reeks DG, Parton TA, King LM, Smith BJ, Lang V, Stephens PE.

J Immunol Methods. 1998 Aug 1;217(1-2):1-10.


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