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J Virol. 2000 Nov;74(22):10816-8.

Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts.

Author information

1
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA. wbresnahan@molbio.princeton.edu

Abstract

A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

PMID:
11044129
PMCID:
PMC110959
DOI:
10.1128/jvi.74.22.10816-10818.2000
[Indexed for MEDLINE]
Free PMC Article

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