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Items: 1 to 50 of 62

1.

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine.

Whale AS, Jones GM, Pavšič J, Dreo T, Redshaw N, Akyürek S, Akgöz M, Divieto C, Sassi MP, He HJ, Cole KD, Bae YK, Park SR, Deprez L, Corbisier P, Garrigou S, Taly V, Larios R, Cowen S, O'Sullivan DM, Bushell CA, Goenaga-Infante H, Foy CA, Woolford AJ, Parkes H, Huggett JF, Devonshire AS.

Clin Chem. 2018 Sep;64(9):1296-1307. doi: 10.1373/clinchem.2017.285478. Epub 2018 Jun 14.

PMID:
29903874
2.

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

Gale D, Lawson ARJ, Howarth K, Madi M, Durham B, Smalley S, Calaway J, Blais S, Jones G, Clark J, Dimitrov P, Pugh M, Woodhouse S, Epstein M, Fernandez-Gonzalez A, Whale AS, Huggett JF, Foy CA, Jones GM, Raveh-Amit H, Schmitt K, Devonshire A, Green E, Forshew T, Plagnol V, Rosenfeld N.

PLoS One. 2018 Mar 16;13(3):e0194630. doi: 10.1371/journal.pone.0194630. eCollection 2018.

3.

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

Busby E, Whale AS, Ferns RB, Grant PR, Morley G, Campbell J, Foy CA, Nastouli E, Huggett JF, Garson JA.

Sci Rep. 2017 Apr 26;7(1):1209. doi: 10.1038/s41598-017-01221-5.

4.

Reproducibility of biomedical research - The importance of editorial vigilance.

Bustin SA, Huggett JF.

Biomol Detect Quantif. 2017 Feb 21;11:1-3. doi: 10.1016/j.bdq.2017.01.002. eCollection 2017 Mar.

5.

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

Yoo HB, Park SR, Dong L, Wang J, Sui Z, Pavšič J, Milavec M, Akgoz M, Mozioğlu E, Corbisier P, Janka M, Cosme B, de V Cavalcante JJ, Flatshart RB, Burke D, Forbes-Smith M, McLaughlin J, Emslie K, Whale AS, Huggett JF, Parkes H, Kline MC, Harenza JL, Vallone PM.

Anal Chem. 2016 Dec 20;88(24):12169-12176. doi: 10.1021/acs.analchem.6b03076. Epub 2016 Nov 30.

PMID:
28193036
6.

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

Pavšič J, Devonshire A, Blejec A, Foy CA, Van Heuverswyn F, Jones GM, Schimmel H, Žel J, Huggett JF, Redshaw N, Karczmarczyk M, Mozioğlu E, Akyürek S, Akgöz M, Milavec M.

Anal Bioanal Chem. 2017 Apr;409(10):2601-2614. doi: 10.1007/s00216-017-0206-0. Epub 2017 Jan 26.

7.

The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs.

Phelan J, O'Sullivan DM, Machado D, Ramos J, Whale AS, O'Grady J, Dheda K, Campino S, McNerney R, Viveiros M, Huggett JF, Clark TG.

Genome Med. 2016 Dec 22;8(1):132. doi: 10.1186/s13073-016-0385-x.

8.

Fundamentals of multiplexing with digital PCR.

Whale AS, Huggett JF, Tzonev S.

Biomol Detect Quantif. 2016 May 27;10:15-23. eCollection 2016 Dec. Review.

9.

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia.

Alikian M, Whale AS, Akiki S, Piechocki K, Torrado C, Myint T, Cowen S, Griffiths M, Reid AG, Apperley J, White H, Huggett JF, Foroni L.

Clin Chem. 2017 Feb;63(2):525-531. doi: 10.1373/clinchem.2016.262824. Epub 2016 Dec 15.

10.

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant.

Whale AS, Devonshire AS, Karlin-Neumann G, Regan J, Javier L, Cowen S, Fernandez-Gonzalez A, Jones GM, Redshaw N, Beck J, Berger AW, Combaret V, Dahl Kjersgaard N, Davis L, Fina F, Forshew T, Fredslund Andersen R, Galbiati S, González Hernández Á, Haynes CA, Janku F, Lacave R, Lee J, Mistry V, Pender A, Pradines A, Proudhon C, Saal LH, Stieglitz E, Ulrich B, Foy CA, Parkes H, Tzonev S, Huggett JF.

Anal Chem. 2017 Feb 7;89(3):1724-1733. doi: 10.1021/acs.analchem.6b03980. Epub 2017 Jan 18.

PMID:
27935690
11.

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis.

Devonshire AS, O'Sullivan DM, Honeyborne I, Jones G, Karczmarczyk M, Pavšič J, Gutteridge A, Milavec M, Mendoza P, Schimmel H, Van Heuverswyn F, Gorton R, Cirillo DM, Borroni E, Harris K, Barnard M, Heydenrych A, Ndusilo N, Wallis CL, Pillay K, Barry T, Reddington K, Richter E, Mozioğlu E, Akyürek S, Yalçınkaya B, Akgoz M, Žel J, Foy CA, McHugh TD, Huggett JF.

BMC Infect Dis. 2016 Aug 3;16:366. doi: 10.1186/s12879-016-1696-7.

12.

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

Devonshire AS, Sanders R, Whale AS, Nixon GJ, Cowen S, Ellison SL, Parkes H, Pine PS, Salit M, McDaniel J, Munro S, Lund S, Matsukura S, Sekiguchi Y, Kawaharasaki M, Granjeiro JM, Falagan-Lotsch P, Saraiva AM, Couto P, Yang I, Kwon H, Park SR, Demšar T, Žel J, Blejec A, Milavec M, Dong L, Zhang L, Sui Z, Wang J, Viroonudomphol D, Prawettongsopon C, Partis L, Baoutina A, Emslie K, Takatsu A, Akyurek S, Akgoz M, Vonsky M, Konopelko LA, Cundapi EM, Urquiza MP, Huggett JF, Foy CA.

Biomol Detect Quantif. 2016 Jun 6;8:15-28. doi: 10.1016/j.bdq.2016.05.003. eCollection 2016 Jun.

13.

qPCR, dPCR, NGS - A journey.

Huggett JF, O'Grady J, Bustin S.

Biomol Detect Quantif. 2015 Jan 15;3:A1-5. doi: 10.1016/j.bdq.2015.01.001. eCollection 2015 Mar. No abstract available.

14.

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.

Alikian M, Ellery P, Forbes M, Gerrard G, Kasperaviciute D, Sosinsky A, Mueller M, Whale AS, Milojkovic D, Apperley J, Huggett JF, Foroni L, Reid AG.

J Mol Diagn. 2016 Mar;18(2):176-89. doi: 10.1016/j.jmoldx.2015.09.005. Epub 2016 Feb 5.

15.

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Whale AS, Bushell CA, Grant PR, Cowen S, Gutierrez-Aguirre I, O'Sullivan DM, Žel J, Milavec M, Foy CA, Nastouli E, Garson JA, Huggett JF.

J Clin Microbiol. 2016 Feb;54(2):392-400. doi: 10.1128/JCM.02611-15. Epub 2015 Dec 9.

16.

[The digital polymerase chain reaction as a new technology and its potential input in molecular diagnostic].

Huggett JF, Whale AS.

Klin Lab Diagn. 2014 Nov;59(11):6-8. Russian. No abstract available.

PMID:
25850236
17.

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

Devonshire AS, Honeyborne I, Gutteridge A, Whale AS, Nixon G, Wilson P, Jones G, McHugh TD, Foy CA, Huggett JF.

Anal Chem. 2015 Apr 7;87(7):3706-13. doi: 10.1021/ac5041617. Epub 2015 Mar 10.

PMID:
25646934
18.

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis.

Redshaw N, Huggett JF, Taylor MS, Foy CA, Devonshire AS.

BMC Genomics. 2014 Dec 23;15:1174. doi: 10.1186/1471-2164-15-1174.

19.

Making standards for quantitative real-time pneumococcal PCR.

Morpeth SC, Huggett JF, Murdoch DR, Scott JA.

Biomol Detect Quantif. 2014 Dec 4;2:1-3. eCollection 2014 Dec.

20.

Assessing the accuracy of quantitative molecular microbial profiling.

O'Sullivan DM, Laver T, Temisak S, Redshaw N, Harris KA, Foy CA, Studholme DJ, Huggett JF.

Int J Mol Sci. 2014 Nov 21;15(11):21476-91. doi: 10.3390/ijms151121476.

21.

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

Nixon GJ, Svenstrup HF, Donald CE, Carder C, Stephenson JM, Morris-Jones S, Huggett JF, Foy CA.

Biomol Detect Quantif. 2014 Nov 22;2:4-10. eCollection 2014 Dec.

22.

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

Pavšič J, Devonshire AS, Parkes H, Schimmel H, Foy CA, Karczmarczyk M, Gutiérrez-Aguirre I, Honeyborne I, Huggett JF, McHugh TD, Milavec M, Zeichhardt H, Žel J.

J Clin Microbiol. 2015 Jul;53(7):2008-14. doi: 10.1128/JCM.02136-14. Epub 2014 Nov 12. Review.

23.

Considerations for digital PCR as an accurate molecular diagnostic tool.

Huggett JF, Cowen S, Foy CA.

Clin Chem. 2015 Jan;61(1):79-88. doi: 10.1373/clinchem.2014.221366. Epub 2014 Oct 22. Review.

24.

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

Marks DJ, Hyams C, Koo CY, Pavlou M, Robbins J, Koo CS, Rodger G, Huggett JF, Yap J, Macrae MB, Swanton RH, Zumla AI, Miller RF.

QJM. 2015 Mar;108(3):219-29. doi: 10.1093/qjmed/hcu188. Epub 2014 Sep 15.

PMID:
25223570
25.

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.

Sanders R, Mason DJ, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6471-83. doi: 10.1007/s00216-014-7857-x. Epub 2014 May 25. Review.

26.

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification.

Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6499-512. doi: 10.1007/s00216-014-7835-3. Epub 2014 May 24.

27.
28.

Digital PCR as a novel technology and its potential implications for molecular diagnostics.

Huggett JF, Whale A.

Clin Chem. 2013 Dec;59(12):1691-3. doi: 10.1373/clinchem.2013.214742. Epub 2013 Oct 7. No abstract available.

29.

Evaluation of digital PCR for absolute RNA quantification.

Sanders R, Mason DJ, Foy CA, Huggett JF.

PLoS One. 2013 Sep 20;8(9):e75296. doi: 10.1371/journal.pone.0075296. eCollection 2013.

30.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

31.

Methods for applying accurate digital PCR analysis on low copy DNA samples.

Whale AS, Cowen S, Foy CA, Huggett JF.

PLoS One. 2013;8(3):e58177. doi: 10.1371/journal.pone.0058177. Epub 2013 Mar 5.

32.

Progress in metagenomics requires a balanced appraisal of the available technologies.

Huggett JF, Studholme DJ, Laver T, Foy CA.

Eur J Clin Microbiol Infect Dis. 2013 Aug;32(8):1097-8. doi: 10.1007/s10096-013-1842-8. Epub 2013 Feb 23. No abstract available.

PMID:
23435754
33.

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis.

Devonshire AS, Sanders R, Wilkes TM, Taylor MS, Foy CA, Huggett JF.

Methods. 2013 Jan;59(1):89-100. doi: 10.1016/j.ymeth.2012.07.021. Epub 2012 Jul 24. Review.

PMID:
22841564
34.

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.

Whale AS, Huggett JF, Cowen S, Speirs V, Shaw J, Ellison S, Foy CA, Scott DJ.

Nucleic Acids Res. 2012 Jun;40(11):e82. doi: 10.1093/nar/gks203. Epub 2012 Feb 28.

35.

Evaluation of digital PCR for absolute DNA quantification.

Sanders R, Huggett JF, Bushell CA, Cowen S, Scott DJ, Foy CA.

Anal Chem. 2011 Sep 1;83(17):6474-84. doi: 10.1021/ac103230c. Epub 2011 Aug 1.

PMID:
21446772
36.

Seasonal variation in mortality of Pneumocystis jirovecii pneumonia in HIV-infected patients.

Miller RF, Evans HE, Copas AJ, Huggett JF, Edwards SG, Walzer PD.

Int J STD AIDS. 2010 Jul;21(7):497-503. doi: 10.1258/ijsa.2010.010148.

PMID:
20852200
37.

Pneumocystis jirovecii in pleural infection: a nucleic acid amplification study.

Wrightson JM, Rahman NM, Novak T, Huggett JF, Maskell NA, Zumla A, Miller RF, Davies RJ.

Thorax. 2011 May;66(5):450-1. doi: 10.1136/thx.2009.129940. Epub 2010 Aug 30. No abstract available.

PMID:
20805155
38.

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis.

Pooran A, Booth H, Miller RF, Scott G, Badri M, Huggett JF, Rook G, Zumla A, Dheda K.

BMC Pulm Med. 2010 Feb 22;10:7. doi: 10.1186/1471-2466-10-7.

39.

An assessment of air as a source of DNA contamination encountered when performing PCR.

Witt N, Rodger G, Vandesompele J, Benes V, Zumla A, Rook GA, Huggett JF.

J Biomol Tech. 2009 Dec;20(5):236-40.

40.

Implications of storing urinary DNA from different populations for molecular analyses.

Cannas A, Kalunga G, Green C, Calvo L, Katemangwe P, Reither K, Perkins MD, Maboko L, Hoelscher M, Talbot EA, Mwaba P, Zumla AI, Girardi E, Huggett JF; TB trDNA consortium.

PLoS One. 2009 Sep 10;4(9):e6985. doi: 10.1371/journal.pone.0006985.

41.

Low sensitivity of a urine LAM-ELISA in the diagnosis of pulmonary tuberculosis.

Reither K, Saathoff E, Jung J, Minja LT, Kroidl I, Saad E, Huggett JF, Ntinginya EN, Maganga L, Maboko L, Hoelscher M.

BMC Infect Dis. 2009 Aug 28;9:141. doi: 10.1186/1471-2334-9-141.

42.

Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods.

Green C, Huggett JF, Talbot E, Mwaba P, Reither K, Zumla AI.

Lancet Infect Dis. 2009 Aug;9(8):505-11. doi: 10.1016/S1473-3099(09)70149-5. Review. Erratum in: Lancet Infect Dis. 2009 Sep;9(9):536.

PMID:
19628175
43.

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

Garson JA, Huggett JF, Bustin SA, Pfaffl MW, Benes V, Vandesompele J, Shipley GL.

AIDS Res Hum Retroviruses. 2009 Mar;25(3):377-8; author reply 379-81. doi: 10.1089/aid.2008.0270. No abstract available.

PMID:
19292592
44.

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon.

Huggett JF, Novak T, Garson JA, Green C, Morris-Jones SD, Miller RF, Zumla A.

BMC Res Notes. 2008 Aug 28;1:70. doi: 10.1186/1756-0500-1-70.

45.

Gene expression of IL17 and IL23 in the lungs of patients with active tuberculosis.

Dheda K, Chang JS, Lala S, Huggett JF, Zumla A, Rook GA.

Thorax. 2008 Jun;63(6):566-8. doi: 10.1136/thx.2007.092205. No abstract available.

PMID:
18511642
46.

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis.

Lala S, Dheda K, Chang JS, Huggett JF, Kim LU, Johnson MA, Rook GA, Keshav S, Zumla A.

BMC Infect Dis. 2007 Aug 16;7:96.

47.

Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveolar lavage fluid of HIV-infected patients.

Huggett JF, Taylor MS, Kocjan G, Evans HE, Morris-Jones S, Gant V, Novak T, Costello AM, Zumla A, Miller RF.

Thorax. 2008 Feb;63(2):154-9. Epub 2007 Aug 10. Erratum in: Thorax. 2009 Jul;64(7):643.

PMID:
17693588
48.

The stability of mRNA encoding IL-4 is increased in pulmonary tuberculosis, while stability of mRNA encoding the antagonistic splice variant, IL-4delta2, is not.

Dheda K, Chang JS, Huggett JF, Kim LU, Johnson MA, Zumla A, Rook GA.

Tuberculosis (Edinb). 2007 May;87(3):237-41. Epub 2006 Dec 29.

PMID:
17196882
49.

Problems of developing molecular diagnostic tests for opportunistic pathogens: the example of Pneumocystis jirovecii.

Huggett JF, Miller RF, Taylor MS, Costello AM, Zumla A.

J Eukaryot Microbiol. 2006;53 Suppl 1:S85-6. No abstract available.

PMID:
17169078
50.

Expression of IL-4 mRNA in peripheral blood mononuclear cells from normal donors in relation to expression of TLR2.

Chang JS, Dheda K, Huggett JF, Kim LU, Zumla A, Rook GA.

Immunol Lett. 2006 Aug 15;106(2):194-7. Epub 2006 Jun 27.

PMID:
16919700

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