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Items: 22

1.

Kinetic modeling of the Calvin cycle identifies flux control and stable metabolomes in Synechocystis carbon fixation.

Janasch M, Asplund-Samuelsson J, Steuer R, Hudson EP.

J Exp Bot. 2019 Feb 5;70(3):973-983. doi: 10.1093/jxb/ery382.

2.

Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins.

Jahn M, Vialas V, Karlsen J, Maddalo G, Edfors F, Forsström B, Uhlén M, Käll L, Hudson EP.

Cell Rep. 2018 Oct 9;25(2):478-486.e8. doi: 10.1016/j.celrep.2018.09.040.

3.

Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound.

Englund E, Shabestary K, Hudson EP, Lindberg P.

Metab Eng. 2018 Sep;49:164-177. doi: 10.1016/j.ymben.2018.07.004. Epub 2018 Jul 17.

4.

Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria.

Shabestary K, Anfelt J, Ljungqvist E, Jahn M, Yao L, Hudson EP.

ACS Synth Biol. 2018 Jul 20;7(7):1669-1675. doi: 10.1021/acssynbio.8b00056. Epub 2018 Jun 8.

PMID:
29874914
5.

Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1.

Cengic I, Uhlén M, Hudson EP.

J Bacteriol. 2018 Jul 25;200(16). pii: e00270-18. doi: 10.1128/JB.00270-18. Print 2018 Aug 15.

6.

Thermodynamic analysis of computed pathways integrated into the metabolic networks of E. coli and Synechocystis reveals contrasting expansion potential.

Asplund-Samuelsson J, Janasch M, Hudson EP.

Metab Eng. 2018 Jan;45:223-236. doi: 10.1016/j.ymben.2017.12.011. Epub 2017 Dec 24.

7.

Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX.

Kaczmarzyk D, Cengic I, Yao L, Hudson EP.

Metab Eng. 2018 Jan;45:59-66. doi: 10.1016/j.ymben.2017.11.014. Epub 2017 Dec 2.

PMID:
29199103
8.

Affibody Scaffolds Improve Sesquiterpene Production in Saccharomyces cerevisiae.

Tippmann S, Anfelt J, David F, Rand JM, Siewers V, Uhlén M, Nielsen J, Hudson EP.

ACS Synth Biol. 2017 Jan 20;6(1):19-28. doi: 10.1021/acssynbio.6b00109. Epub 2016 Aug 30.

PMID:
27560952
9.

Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis.

Shabestary K, Hudson EP.

Metab Eng Commun. 2016 Jul 20;3:216-226. doi: 10.1016/j.meteno.2016.07.003. eCollection 2016 Dec.

10.

Arabidopsis acyl-acyl carrier protein synthetase AAE15 with medium chain fatty acid specificity is functional in cyanobacteria.

Kaczmarzyk D, Hudson EP, Fulda M.

AMB Express. 2016 Mar;6(1):7. doi: 10.1186/s13568-016-0178-z. Epub 2016 Jan 21.

11.

Multiple Gene Repression in Cyanobacteria Using CRISPRi.

Yao L, Cengic I, Anfelt J, Hudson EP.

ACS Synth Biol. 2016 Mar 18;5(3):207-12. doi: 10.1021/acssynbio.5b00264. Epub 2015 Dec 28.

PMID:
26689101
12.

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria.

Hammar P, Angermayr SA, Sjostrom SL, van der Meer J, Hellingwerf KJ, Hudson EP, Joensson HN.

Biotechnol Biofuels. 2015 Nov 25;8:193. doi: 10.1186/s13068-015-0380-2. eCollection 2015.

13.

Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production.

Anfelt J, Kaczmarzyk D, Shabestary K, Renberg B, Rockberg J, Nielsen J, Uhlén M, Hudson EP.

Microb Cell Fact. 2015 Oct 16;14:167. doi: 10.1186/s12934-015-0355-9.

14.

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts.

Lundqvist M, Edfors F, Sivertsson Å, Hallström BM, Hudson EP, Tegel H, Holmberg A, Uhlén M, Rockberg J.

Nucleic Acids Res. 2015 Apr 20;43(7):e49. doi: 10.1093/nar/gkv036. Epub 2015 Jan 23.

15.

Overexpression of sigma factor SigB improves temperature and butanol tolerance of Synechocystis sp. PCC6803.

Kaczmarzyk D, Anfelt J, Särnegrim A, Hudson EP.

J Biotechnol. 2014 Jul 20;182-183:54-60. doi: 10.1016/j.jbiotec.2014.04.017. Epub 2014 May 5.

PMID:
24800959
16.

Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays.

Forsström B, Axnäs BB, Stengele KP, Bühler J, Albert TJ, Richmond TA, Hu FJ, Nilsson P, Hudson EP, Rockberg J, Uhlen M.

Mol Cell Proteomics. 2014 Jun;13(6):1585-97. doi: 10.1074/mcp.M113.033308. Epub 2014 Apr 4.

17.

Using transcriptomics to improve butanol tolerance of Synechocystis sp. strain PCC 6803.

Anfelt J, Hallström B, Nielsen J, Uhlén M, Hudson EP.

Appl Environ Microbiol. 2013 Dec;79(23):7419-27. doi: 10.1128/AEM.02694-13. Epub 2013 Sep 20.

18.

Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes.

Hudson EP, Uhlen M, Rockberg J.

Sci Rep. 2012;2:706. doi: 10.1038/srep00706. Epub 2012 Oct 4.

19.

Automated solid-phase subcloning based on beads brought into proximity by magnetic force.

Hudson EP, Nikoshkov A, Uhlen M, Rockberg J.

PLoS One. 2012;7(5):e37429. doi: 10.1371/journal.pone.0037429. Epub 2012 May 18.

20.

Active-site motions and polarity enhance catalytic turnover of hydrated subtilisin dissolved in organic solvents.

Hudson EP, Eppler RK, Beaudoin JM, Dordick JS, Reimer JA, Clark DS.

J Am Chem Soc. 2009 Apr 1;131(12):4294-300. doi: 10.1021/ja806996q.

21.

Biocatalyst activity in nonaqueous environments correlates with centisecond-range protein motions.

Eppler RK, Hudson EP, Chase SD, Dordick JS, Reimer JA, Clark DS.

Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15672-7. doi: 10.1073/pnas.0804566105. Epub 2008 Oct 7.

22.

Biocatalysis in semi-aqueous and nearly anhydrous conditions.

Hudson EP, Eppler RK, Clark DS.

Curr Opin Biotechnol. 2005 Dec;16(6):637-43. Epub 2005 Oct 26. Review.

PMID:
16256329

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