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Items: 32


The predominance of nucleotidyl activation in bacterial phosphonate biosynthesis.

Rice K, Batul K, Whiteside J, Kelso J, Papinski M, Schmidt E, Pratasouskaya A, Wang D, Sullivan R, Bartlett C, Weadge JT, Van der Kamp MW, Moreno-Hagelsieb G, Suits MD, Horsman GP.

Nat Commun. 2019 Aug 16;10(1):3698. doi: 10.1038/s41467-019-11627-6.


An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters.

Hao T, Xie Z, Wang M, Liu L, Zhang Y, Wang W, Zhang Z, Zhao X, Li P, Guo Z, Gao S, Lou C, Zhang G, Merritt J, Horsman GP, Chen Y.

Nat Commun. 2019 Aug 14;10(1):3665. doi: 10.1038/s41467-019-11673-0.


PokMT1 from the Polyketomycin Biosynthetic Machinery of Streptomyces diastatochromogenes Tü6028 Belongs to the Emerging Family of C-Methyltransferases That Act on CoA-Activated Aromatic Substrates.

Guo X, Crnovcic I, Chang CY, Luo J, Lohman JR, Papinski M, Bechthold A, Horsman GP, Shen B.

Biochemistry. 2018 Feb 13;57(6):1003-1011. doi: 10.1021/acs.biochem.7b01219. Epub 2018 Jan 30.


Whole-Cell Detection of C-P Bonds in Bacteria.

Bartlett C, Bansal S, Burnett A, Suits MD, Schaefer J, Cegelski L, Horsman GP, Weadge JT.

Biochemistry. 2017 Nov 7;56(44):5870-5873. doi: 10.1021/acs.biochem.7b00814.


Genome mining unveils widespread natural product biosynthetic capacity in human oral microbe Streptococcus mutans.

Liu L, Hao T, Xie Z, Horsman GP, Chen Y.

Sci Rep. 2016 Nov 21;6:37479. doi: 10.1038/srep37479.


Phosphonate Biochemistry.

Horsman GP, Zechel DL.

Chem Rev. 2017 Apr 26;117(8):5704-5783. doi: 10.1021/acs.chemrev.6b00536. Epub 2016 Oct 27. Review.


Non-enzymatic pyridine ring formation in the biosynthesis of the rubrolone tropolone alkaloids.

Yan Y, Yang J, Yu Z, Yu M, Ma YT, Wang L, Su C, Luo J, Horsman GP, Huang SX.

Nat Commun. 2016 Oct 7;7:13083. doi: 10.1038/ncomms13083.


Tropolone Ring Construction in the Biosynthesis of Rubrolone B, a Cationic Tropolone Alkaloid from Endophytic Streptomyces.

Yan Y, Ma YT, Yang J, Horsman GP, Luo D, Ji X, Huang SX.

Org Lett. 2016 Mar 18;18(6):1254-7. doi: 10.1021/acs.orglett.6b00074. Epub 2016 Feb 29.


Evaporative light scattering quantification of natural products possessing a carbon-phosphorus bond.

Chi MC, Horsman GP.

J Antibiot (Tokyo). 2015 Dec;68(12):752-6. doi: 10.1038/ja.2015.62. Epub 2015 May 20. No abstract available.


Predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of nine-membered enediyne antitumor antibiotics.

Horsman GP, Lechner A, Ohnishi Y, Moore BS, Shen B.

Biochemistry. 2013 Aug 6;52(31):5217-24. doi: 10.1021/bi400572a. Epub 2013 Jul 23.


Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics.

Lohman JR, Huang SX, Horsman GP, Dilfer PE, Huang T, Chen Y, Wendt-Pienkowski E, Shen B.

Mol Biosyst. 2013 Mar;9(3):478-91. doi: 10.1039/c3mb25523a. Epub 2013 Jan 29.


The catalytic serine of meta-cleavage product hydrolases is activated differently for C-O bond cleavage than for C-C bond cleavage.

Ruzzini AC, Horsman GP, Eltis LD.

Biochemistry. 2012 Jul 24;51(29):5831-40. doi: 10.1021/bi300663r. Epub 2012 Jul 11.


Specificity of the ester bond forming condensation enzyme SgcC5 in C-1027 biosynthesis.

Lin S, Huang T, Horsman GP, Huang SX, Guo X, Shen B.

Org Lett. 2012 May 4;14(9):2300-3. doi: 10.1021/ol300720s. Epub 2012 Apr 20.


Identification of an acyl-enzyme intermediate in a meta-cleavage product hydrolase reveals the versatility of the catalytic triad.

Ruzzini AC, Ghosh S, Horsman GP, Foster LJ, Bolin JT, Eltis LD.

J Am Chem Soc. 2012 Mar 14;134(10):4615-24. doi: 10.1021/ja208544g. Epub 2012 Mar 5.


Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus.

Chen Y, Yin M, Horsman GP, Shen B.

J Nat Prod. 2011 Mar 25;74(3):420-4. doi: 10.1021/np100825y. Epub 2011 Jan 21.


Enediyne antitumor antibiotic maduropeptin biosynthesis featuring a C-methyltransferase that acts on a CoA-tethered aromatic substrate.

Ling J, Horsman GP, Huang SX, Luo Y, Lin S, Shen B.

J Am Chem Soc. 2010 Sep 15;132(36):12534-6. doi: 10.1021/ja1050814.


Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster.

Lin S, Horsman GP, Shen B.

Org Lett. 2010 Sep 3;12(17):3816-9. doi: 10.1021/ol101473t.


Manipulation of pathway regulation in Streptomyces globisporus for overproduction of the enediyne antitumor antibiotic C-1027.

Chen Y, Yin M, Horsman GP, Huang S, Shen B.

J Antibiot (Tokyo). 2010 Aug;63(8):482-5. doi: 10.1038/ja.2010.55. Epub 2010 Jun 16.


Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes.

Horsman GP, Chen Y, Thorson JS, Shen B.

Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11331-5. doi: 10.1073/pnas.1003442107. Epub 2010 Jun 7.


Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism.

Lack NA, Yam KC, Lowe ED, Horsman GP, Owen RL, Sim E, Eltis LD.

J Biol Chem. 2010 Jan 1;285(1):434-43. doi: 10.1074/jbc.M109.058081. Epub 2009 Oct 29.


Characterization of the SgcF epoxide hydrolase supporting an (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis.

Lin S, Horsman GP, Chen Y, Li W, Shen B.

J Am Chem Soc. 2009 Nov 18;131(45):16410-7. doi: 10.1021/ja901242s.


Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027.

Van Lanen SG, Lin S, Horsman GP, Shen B.

FEMS Microbiol Lett. 2009 Nov;300(2):237-41. doi: 10.1111/j.1574-6968.2009.01802.x. Epub 2009 Sep 27.


Iterative type I polyketide synthases for enediyne core biosynthesis.

Horsman GP, Van Lanen SG, Shen B.

Methods Enzymol. 2009;459:97-112. doi: 10.1016/S0076-6879(09)04605-9.


The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad.

Horsman GP, Bhowmik S, Seah SY, Kumar P, Bolin JT, Eltis LD.

J Biol Chem. 2007 Jul 6;282(27):19894-904. Epub 2007 Apr 18.


Characterization of a C-C bond hydrolase from Sphingomonas wittichii RW1 with novel specificities towards polychlorinated biphenyl metabolites.

Seah SY, Ke J, Denis G, Horsman GP, Fortin PD, Whiting CJ, Eltis LD.

J Bacteriol. 2007 Jun;189(11):4038-45. Epub 2007 Apr 6.


Kinetic and structural insight into the mechanism of BphD, a C-C bond hydrolase from the biphenyl degradation pathway.

Horsman GP, Ke J, Dai S, Seah SY, Bolin JT, Eltis LD.

Biochemistry. 2006 Sep 19;45(37):11071-86.


Spectroscopic studies of the anaerobic enzyme-substrate complex of catechol 1,2-dioxygenase.

Horsman GP, Jirasek A, Vaillancourt FH, Barbosa CJ, Jarzecki AA, Xu C, Mekmouche Y, Spiro TG, Lipscomb JD, Blades MW, Turner RF, Eltis LD.

J Am Chem Soc. 2005 Dec 7;127(48):16882-91.


Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations.

Park S, Morley KL, Horsman GP, Holmquist M, Hult K, Kazlauskas RJ.

Chem Biol. 2005 Jan;12(1):45-54.


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