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Items: 1 to 50 of 89

1.

Surveillance of Foodborne Pathogens: Towards Diagnostic Metagenomics of Fecal Samples.

Andersen SC, Hoorfar J.

Genes (Basel). 2018 Jan 4;9(1). pii: E14. doi: 10.3390/genes9010014. Review.

2.

Gene-Based Pathogen Detection: Can We Use qPCR to Predict the Outcome of Diagnostic Metagenomics?

Andersen SC, Fachmann MSR, Kiil K, Møller Nielsen E, Hoorfar J.

Genes (Basel). 2017 Nov 20;8(11). pii: E332. doi: 10.3390/genes8110332.

3.

Towards diagnostic metagenomics of Campylobacter in fecal samples.

Andersen SC, Kiil K, Harder CB, Josefsen MH, Persson S, Nielsen EM, Hoorfar J.

BMC Microbiol. 2017 Jun 8;17(1):133. doi: 10.1186/s12866-017-1041-3.

4.

Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method.

Fachmann MSR, Löfström C, Hoorfar J, Hansen F, Christensen J, Mansdal S, Josefsen MH.

Appl Environ Microbiol. 2017 Feb 15;83(5). pii: e03151-16. doi: 10.1128/AEM.03151-16. Print 2017 Mar 1.

5.

Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases.

Fachmann MS, Josefsen MH, Hoorfar J, Nielsen MT, Löfström C.

J Appl Microbiol. 2015 Nov;119(5):1391-402. doi: 10.1111/jam.12937. Epub 2015 Sep 24.

6.

Microbial food safety: Potential of DNA extraction methods for use in diagnostic metagenomics.

Josefsen MH, Andersen SC, Christensen J, Hoorfar J.

J Microbiol Methods. 2015 Jul;114:30-4. doi: 10.1016/j.mimet.2015.04.016. Epub 2015 May 1.

PMID:
25937085
7.

Monitoring Campylobacter in the poultry production chain—from culture to genes and beyond.

Josefsen MH, Bhunia AK, Engvall EO, Fachmann MS, Hoorfar J.

J Microbiol Methods. 2015 May;112:118-25. doi: 10.1016/j.mimet.2015.03.007. Epub 2015 Mar 11. Review.

PMID:
25771343
8.

Intestinal colonization of broiler chickens by Campylobacter spp. in an experimental infection study.

Bahrndorff S, Garcia AB, Vigre H, Nauta M, Heegaard PM, Madsen M, Hoorfar J, Hald B.

Epidemiol Infect. 2015 Aug;143(11):2381-9. doi: 10.1017/S0950268814003239. Epub 2014 Dec 4.

PMID:
25471550
9.

Low-cost monitoring of Campylobacter in poultry houses by air sampling and quantitative PCR.

Søndergaard MS, Josefsen MH, Löfström C, Christensen LS, Wieczorek K, Osek J, Hoorfar J.

J Food Prot. 2014 Feb;77(2):325-30. doi: 10.4315/0362-028X.JFP-13-268.

PMID:
24490929
10.

Enumeration of salmonellae in table eggs, pasteurized egg products, and egg-containing dishes by using quantitative real-time PCR.

Jakociune D, Pasquali F, da Silva CS, Löfström C, Hoorfar J, Klein G, Manfreda G, Olsen JE.

Appl Environ Microbiol. 2014 Mar;80(5):1616-22. doi: 10.1128/AEM.03360-13. Epub 2013 Dec 20.

11.

Evaluation of direct 16S rDNA sequencing as a metagenomics-based approach to screening bacteria in bottled water.

Hansen T, Skånseng B, Hoorfar J, Löfström C.

Biosecur Bioterror. 2013 Sep;11 Suppl 1:S158-65. doi: 10.1089/bsp.2012.0073.

PMID:
23971801
12.

Evaluation of pre-PCR processing approaches for enumeration of Salmonella enterica in naturally contaminated animal feed.

Schelin J, Andersson G, Vigre H, Norling B, Häggblom P, Hoorfar J, Rådström P, Löfström C.

J Appl Microbiol. 2014 Jan;116(1):167-78. doi: 10.1111/jam.12337. Epub 2013 Sep 24.

13.

Design and data analysis of experimental trials to test vaccine candidates against zoonotic pathogens in animals: the case of a clinical trial against campylobacter in broilers.

Garcia AB, Bahrndorff S, Hald B, Hoorfar J, Madsen M, Vigre H.

Expert Rev Vaccines. 2012 Oct;11(10):1179-88. doi: 10.1586/erv.12.98. Review.

PMID:
23176651
14.
15.

Validation of an open-formula, diagnostic real-time PCR method for 20-h detection of Salmonella in animal feeds.

Löfström C, Hoorfar J.

Vet Microbiol. 2012 Aug 17;158(3-4):431-5. doi: 10.1016/j.vetmic.2012.02.026. Epub 2012 Feb 25.

PMID:
22437008
16.

Rapid detection, characterization, and enumeration of foodborne pathogens.

Hoorfar J.

APMIS Suppl. 2011 Nov;(133):1-24. doi: 10.1111/j.1600-0463.2011.02767.x. Review.

PMID:
22250747
17.

Direct detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap.

Grønlund H, Moen B, Hoorfar J, Radstrøm P, Malorny B, Rudi K.

Prep Biochem Biotechnol. 2011;41(2):166-74. doi: 10.1080/10826068.2011.547366.

PMID:
21442552
18.

Real-time monitoring of Salmonella enterica in free-range geese.

Christensen LS, Josefsen MH, Pedersen K, Christensen J, Bonnichsen L, Sørensen G, Hoorfar J.

Appl Environ Microbiol. 2011 May;77(9):3160-2. doi: 10.1128/AEM.02356-10. Epub 2011 Mar 4.

19.

Development and evaluation of novel one-step TaqMan realtime RT-PCR assays for the detection and direct genotyping of genogroup I and II noroviruses.

Schultz AC, Vega E, Dalsgaard A, Christensen LS, Nørrung B, Hoorfar J, Vinjé J.

J Clin Virol. 2011 Mar;50(3):230-4. doi: 10.1016/j.jcv.2010.12.001. Epub 2010 Dec 30.

PMID:
21195660
20.

A novel strategy to obtain quantitative data for modelling: combined enrichment and real-time PCR for enumeration of salmonellae from pig carcasses.

Krämer N, Löfström C, Vigre H, Hoorfar J, Bunge C, Malorny B.

Int J Food Microbiol. 2011 Mar 1;145 Suppl 1:S86-95. doi: 10.1016/j.ijfoodmicro.2010.08.026. Epub 2010 Sep 18.

PMID:
20855120
21.

Microarray-based genotyping of Salmonella: inter-laboratory evaluation of reproducibility and standardization potential.

Grønlund H, Riber L, Vigre H, Löfström C, Folling L, Huehn S, Malorny B, Rådström P, Rudi K, Hoorfar J.

Int J Food Microbiol. 2011 Mar 1;145 Suppl 1:S79-85. doi: 10.1016/j.ijfoodmicro.2010.08.007. Epub 2010 Aug 14.

PMID:
20817296
22.

Collaborative validation of a rapid method for efficient virus concentration in bottled water.

Schultz AC, Perelle S, Di Pasquale S, Kovac K, De Medici D, Fach P, Sommer HM, Hoorfar J.

Int J Food Microbiol. 2011 Mar 1;145 Suppl 1:S158-66. doi: 10.1016/j.ijfoodmicro.2010.07.030. Epub 2010 Jul 30.

PMID:
20724015
23.

Towards biotracing in food chains.

Hoorfar J, Wagner M, Jordan K, Bouquin SL, Skiby J.

Int J Food Microbiol. 2011 Mar 1;145 Suppl 1:S1-4. doi: 10.1016/j.ijfoodmicro.2010.04.028. Epub 2010 May 16.

PMID:
20627434
24.

Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment.

Josefsen MH, Löfström C, Hansen TB, Christensen LS, Olsen JE, Hoorfar J.

Appl Environ Microbiol. 2010 Aug;76(15):5097-104. doi: 10.1128/AEM.00411-10. Epub 2010 Jun 18.

25.

Culture-independent quantification of Salmonella enterica in carcass gauze swabs by flotation prior to real-time PCR.

Löfström C, Schelin J, Norling B, Vigre H, Hoorfar J, Rådström P.

Int J Food Microbiol. 2011 Mar 1;145 Suppl 1:S103-9. doi: 10.1016/j.ijfoodmicro.2010.03.042. Epub 2010 Apr 10.

PMID:
20417982
26.

Validation of a 20-h real-time PCR method for screening of Salmonella in poultry faecal samples.

Löfström C, Hansen F, Hoorfar J.

Vet Microbiol. 2010 Aug 26;144(3-4):511-4. doi: 10.1016/j.vetmic.2010.02.019. Epub 2010 Feb 18.

PMID:
20207510
27.

Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella.

Löfström C, Krause M, Josefsen MH, Hansen F, Hoorfar J.

BMC Microbiol. 2009 May 7;9:85. doi: 10.1186/1471-2180-9-85.

28.

Diagnostic PCR: comparative sensitivity of four probe chemistries.

Josefsen MH, Löfström C, Sommer HM, Hoorfar J.

Mol Cell Probes. 2009 Jun-Aug;23(3-4):201-3. doi: 10.1016/j.mcp.2009.02.003. Epub 2009 Mar 6.

PMID:
19269316
29.

Detection of Campylobacter bacteria in air samples for continuous real-time monitoring of Campylobacter colonization in broiler flocks.

Olsen KN, Lund M, Skov J, Christensen LS, Hoorfar J.

Appl Environ Microbiol. 2009 Apr;75(7):2074-8. doi: 10.1128/AEM.02182-08. Epub 2009 Feb 6.

30.

Enumeration of salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk assessment.

Malorny B, Löfström C, Wagner M, Krämer N, Hoorfar J.

Appl Environ Microbiol. 2008 Mar;74(5):1299-304. doi: 10.1128/AEM.02489-07. Epub 2007 Dec 28. Review. No abstract available.

31.

Characterization and biological role of the O-polysaccharide gene cluster of Yersinia enterocolitica serotype O:9.

Skurnik M, Biedzka-Sarek M, Lübeck PS, Blom T, Bengoechea JA, Pérez-Gutiérrez C, Ahrens P, Hoorfar J.

J Bacteriol. 2007 Oct;189(20):7244-53. Epub 2007 Aug 10.

32.

Optimization of a 12-hour TaqMan PCR-based method for detection of Salmonella bacteria in meat.

Josefsen MH, Krause M, Hansen F, Hoorfar J.

Appl Environ Microbiol. 2007 May;73(9):3040-8. Epub 2007 Mar 9.

33.

Comparison of methods for detection of norovirus in oysters.

Schultz AC, Saadbye P, Hoorfar J, Nørrung B.

Int J Food Microbiol. 2007 Mar 20;114(3):352-6. Epub 2006 Dec 19.

PMID:
17182147
34.

Comparative, collaborative, and on-site validation of a TaqMan PCR method as a tool for certified production of fresh, campylobacter-free chickens.

Krause M, Josefsen MH, Lund M, Jacobsen NR, Brorsen L, Moos M, Stockmarr A, Hoorfar J.

Appl Environ Microbiol. 2006 Aug;72(8):5463-8.

35.

Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR.

Reynisson E, Josefsen MH, Krause M, Hoorfar J.

J Microbiol Methods. 2006 Aug;66(2):206-16. Epub 2005 Dec 20.

PMID:
16364478
36.

A real-time PCR assay for the specific identification of serotype O:9 of Yersinia enterocolitica.

Jacobsen NR, Bogdanovich T, Skurnik M, Lübeck PS, Ahrens P, Hoorfar J.

J Microbiol Methods. 2005 Nov;63(2):151-6. Epub 2005 Apr 19.

PMID:
16226639
37.

Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR.

Wolffs P, Norling B, Hoorfar J, Griffiths M, Rådström P.

Appl Environ Microbiol. 2005 Oct;71(10):5759-64.

38.

Toward standardization of diagnostic PCR testing of fecal samples: lessons from the detection of salmonellae in pigs.

Malorny B, Hoorfar J.

J Clin Microbiol. 2005 Jul;43(7):3033-7. Review. No abstract available.

39.

Novel approach for assessing performance of PCR cyclers used for diagnostic testing.

Schoder D, Schmalwieser A, Schauberger G, Hoorfar J, Kuhn M, Wagner M.

J Clin Microbiol. 2005 Jun;43(6):2724-8.

40.

The need for standardization of real-time PCR methods.

Hoorfar J.

J AOAC Int. 2005 Mar-Apr;88(2):45A. No abstract available.

PMID:
15859060
41.

Diagnostic PCR: validation and sample preparation are two sides of the same coin.

Hoorfar J, Wolffs P, Rådström P.

APMIS. 2004 Nov-Dec;112(11-12):808-14. Review.

PMID:
15688523
42.

Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples.

Perelle S, Dilasser F, Malorny B, Grout J, Hoorfar J, Fach P.

Mol Cell Probes. 2004 Dec;18(6):409-20.

PMID:
15488381
43.

A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model--towards an international standard.

D'Agostino M, Wagner M, Vazquez-Boland JA, Kuchta T, Karpiskova R, Hoorfar J, Novella S, Scortti M, Ellison J, Murray A, Fernandes I, Kuhn M, Pazlarova J, Heuvelink A, Cook N.

J Food Prot. 2004 Aug;67(8):1646-55.

PMID:
15330529
44.

Multicenter validation of PCR-based method for detection of Salmonella in chicken and pig samples.

Malorny B, Cook N, D'Agostino M, De Medici D, Croci L, Abdulmawjood A, Fach P, Karpiskova R, Aymerich T, Kwaitek K, Hoorfar J, Malorny B.

J AOAC Int. 2004 Jul-Aug;87(4):861-6.

PMID:
15295881
45.

Toward an international standard for PCR-based detection of foodborne Escherichia coli O157: validation of the PCR-based method in a multicenter interlaboratory trial.

Abdulmawjood A, Bülte M, Roth S, Schönenbrücher H, Cook N, D'Agostino M, Burkhard M, Jordan K, Pelkonen S, Hoorfar J.

J AOAC Int. 2004 Jul-Aug;87(4):856-60.

PMID:
15295880
46.

A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter.

Perelle S, Josefsen M, Hoorfar J, Dilasser F, Grout J, Fach P.

Mol Cell Probes. 2004 Oct;18(5):321-7.

PMID:
15294320
47.

Development, validation, and standardization of polymerase chain reaction-based detection of E. coli O157.

Abdulmawjood A, Bülte M, Roth S, Schönenbrücher H, Cook N, Heuvelink AE, Hoorfar J.

J AOAC Int. 2004 May-Jun;87(3):596-603.

PMID:
15287657
48.

Internal amplification control for PCR should not be mandatory in the clinical medical environment.

Barkham T, Hoorfar J.

J Clin Microbiol. 2004 Jul;42(7):3379-80. No abstract available.

49.

Validation of a PCR-based method for detection of food-borne thermotolerant campylobacters in a multicenter collaborative trial.

Josefsen MH, Cook N, D'Agostino M, Hansen F, Wagner M, Demnerova K, Heuvelink AE, Tassios PT, Lindmark H, Kmet V, Barbanera M, Fach P, Loncarevic S, Hoorfar J.

Appl Environ Microbiol. 2004 Jul;70(7):4379-83.

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