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Items: 40


African Horse Sickness: A Review of Current Understanding and Vaccine Development.

Dennis SJ, Meyers AE, Hitzeroth II, Rybicki EP.

Viruses. 2019 Sep 11;11(9). pii: E844. doi: 10.3390/v11090844. Review.


Substitution of Human Papillomavirus Type 16 L2 Neutralizing Epitopes Into L1 Surface Loops: The Effect on Virus-Like Particle Assembly and Immunogenicity.

Chabeda A, van Zyl AR, Rybicki EP, Hitzeroth II.

Front Plant Sci. 2019 Jun 20;10:779. doi: 10.3389/fpls.2019.00779. eCollection 2019.


Cytological Immunostaining of HMGA2, LRP1B, and TP63 as Potential Biomarkers for Triaging Human Papillomavirus-Positive Women.

Jiang Y, Zhu C, He D, Gao Q, Tian X, Ma X, Wu J, Das BC, Severinov K, Hitzeroth II, Debata PR, Liu R, Zou L, Shi L, Xu H, Wang K, Bao Y, Ka-Kit LR, You Z, Cui Z, Hu Z.

Transl Oncol. 2019 Jul;12(7):959-967. doi: 10.1016/j.tranon.2019.04.012. Epub 2019 May 16.


Risk stratification of cervical lesions using capture sequencing and machine learning method based on HPV and human integrated genomic profiles.

Tian R, Cui Z, He D, Tian X, Gao Q, Ma X, Yang JR, Wu J, Das BC, Severinov K, Hitzeroth II, Debata PR, Xu W, Zhong H, Fan W, Chen Y, Jin Z, Cao C, Yu M, Xie W, Huang Z, Bao Y, Xie H, Yao S, Hu Z.

Carcinogenesis. 2019 May 17. pii: bgz094. doi: 10.1093/carcin/bgz094. [Epub ahead of print]


Immunogenicity of plant-produced porcine circovirus-like particles in mice.

Gunter CJ, Regnard GL, Rybicki EP, Hitzeroth II.

Plant Biotechnol J. 2019 Sep;17(9):1751-1759. doi: 10.1111/pbi.13097. Epub 2019 Mar 10.


Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses.

Dennis SJ, O'Kennedy MM, Rutkowska D, Tsekoa T, Lourens CW, Hitzeroth II, Meyers AE, Rybicki EP.

Vet Res. 2018 Oct 11;49(1):105. doi: 10.1186/s13567-018-0600-4.


Optimizing a Human Papillomavirus Type 16 L1-Based Chimaeric Gene for Expression in Plants.

Hitzeroth II, Chabeda A, Whitehead MP, Graf M, Rybicki EP.

Front Bioeng Biotechnol. 2018 Jul 16;6:101. doi: 10.3389/fbioe.2018.00101. eCollection 2018.


Transient Expression and Purification of Horseradish Peroxidase C in Nicotiana benthamiana.

Huddy SM, Hitzeroth II, Meyers AE, Weber B, Rybicki EP.

Int J Mol Sci. 2018 Jan 1;19(1). pii: E115. doi: 10.3390/ijms19010115.


Therapeutic vaccines for high-risk HPV-associated diseases.

Chabeda A, Yanez RJR, Lamprecht R, Meyers AE, Rybicki EP, Hitzeroth II.

Papillomavirus Res. 2018 Jun;5:46-58. doi: 10.1016/j.pvr.2017.12.006. Epub 2017 Dec 19. Review.


Complete Genome Sequence of Bos taurus Papillomavirus Type 1, Isolated in Morocco.

Regnard GL, Matiso A, Mounir L, Embarki T, Hitzeroth II, Rybicki EP.

Genome Announc. 2017 Sep 21;5(38). pii: e00979-17. doi: 10.1128/genomeA.00979-17.


Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana.

Regnard GL, Rybicki EP, Hitzeroth II.

Virol J. 2017 Sep 11;14(1):174. doi: 10.1186/s12985-017-0847-9.


Xenogenic rolling-circle replication of a synthetic beak and feather disease virus genomic clone in 293TT mammalian cells and Nicotiana benthamiana.

Regnard GL, de Moor WRJ, Hitzeroth II, Williamson AL, Rybicki EP.

J Gen Virol. 2017 Sep;98(9):2329-2338. doi: 10.1099/jgv.0.000915.


Expression optimization of a cell membrane-penetrating human papillomavirus type 16 therapeutic vaccine candidate in Nicotiana benthamiana.

Yanez RJR, Lamprecht R, Granadillo M, Weber B, Torrens I, Rybicki EP, Hitzeroth II.

PLoS One. 2017 Aug 11;12(8):e0183177. doi: 10.1371/journal.pone.0183177. eCollection 2017.


LALF32-51 -E7, a HPV-16 therapeutic vaccine candidate, forms protein body-like structures when expressed in Nicotiana benthamiana leaves.

Yanez RJR, Lamprecht R, Granadillo M, Torrens I, Arcalís E, Stöger E, Rybicki EP, Hitzeroth II.

Plant Biotechnol J. 2018 Feb;16(2):628-637. doi: 10.1111/pbi.12802. Epub 2017 Sep 7.


Immunogenicity of plant-produced African horse sickness virus-like particles: implications for a novel vaccine.

Dennis SJ, Meyers AE, Guthrie AJ, Hitzeroth II, Rybicki EP.

Plant Biotechnol J. 2018 Feb;16(2):442-450. doi: 10.1111/pbi.12783. Epub 2017 Aug 1.


Complete Genome Sequences of Two Isolates of Canis familiaris Oral Papillomavirus from South Africa.

Regnard GL, Baloyi NM, Bracher LR, Hitzeroth II, Rybicki EP.

Genome Announc. 2016 Dec 8;4(6). pii: e01006-16. doi: 10.1128/genomeA.01006-16.


Transient Expression of Viral Proteins in Plants Using Agrobacterium tumefaciens.

Hitzeroth II, van Zyl AR.

Methods Mol Biol. 2016;1404:581-95. doi: 10.1007/978-1-4939-3389-1_38.


Purification of Virus-Like Particles (VLPs) from Plants.

van Zyl AR, Hitzeroth II.

Methods Mol Biol. 2016;1404:569-79. doi: 10.1007/978-1-4939-3389-1_37.


Production of Human papillomavirus pseudovirions in plants and their use in pseudovirion-based neutralisation assays in mammalian cells.

Lamprecht RL, Kennedy P, Huddy SM, Bethke S, Hendrikse M, Hitzeroth II, Rybicki EP.

Sci Rep. 2016 Feb 8;6:20431. doi: 10.1038/srep20431.


Production of H5N1 Influenza Virus Matrix Protein 2 Ectodomain Protein Bodies in Tobacco Plants and in Insect Cells as a Candidate Universal Influenza Vaccine.

Mbewana S, Mortimer E, Pêra FF, Hitzeroth II, Rybicki EP.

Front Bioeng Biotechnol. 2015 Dec 8;3:197. doi: 10.3389/fbioe.2015.00197. eCollection 2015.


Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana.

Pêra FF, Mutepfa DL, Khan AM, Els JH, Mbewana S, van Dijk AA, Rybicki EP, Hitzeroth II.

Virol J. 2015 Dec 2;12:205. doi: 10.1186/s12985-015-0436-8.


Beak and feather disease viruses circulating in Cape parrots (Poicepahlus robustus) in South Africa.

Regnard GL, Boyes RS, Martin RO, Hitzeroth II, Rybicki EP.

Arch Virol. 2015 Jan;160(1):47-54. doi: 10.1007/s00705-014-2226-9. Epub 2014 Sep 11.


Beak and feather disease virus: correlation between viral load and clinical signs in wild Cape parrots (Poicepahlus robustus) in South Africa.

Regnard GL, Boyes RS, Martin RO, Hitzeroth II, Rybicki EP.

Arch Virol. 2015 Jan;160(1):339-44. doi: 10.1007/s00705-014-2225-x. Epub 2014 Sep 7.


Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.

Whitehead M, Ohlschläger P, Almajhdi FN, Alloza L, Marzábal P, Meyers AE, Hitzeroth II, Rybicki EP.

BMC Cancer. 2014 May 24;14:367. doi: 10.1186/1471-2407-14-367.


Immunogenic assessment of plant-produced human papillomavirus type 16 L1/L2 chimaeras.

Pineo CB, Hitzeroth II, Rybicki EP.

Plant Biotechnol J. 2013 Oct;11(8):964-75. doi: 10.1111/pbi.12089. Epub 2013 Aug 7.


Development of human papillomavirus chimaeric L1/L2 candidate vaccines.

McGrath M, de Villiers GK, Shephard E, Hitzeroth II, Rybicki EP.

Arch Virol. 2013 Oct;158(10):2079-88. doi: 10.1007/s00705-013-1713-8. Epub 2013 May 1.


Expression in tobacco and purification of beak and feather disease virus capsid protein fused to elastin-like polypeptides.

Duvenage L, Hitzeroth II, Meyers AE, Rybicki EP.

J Virol Methods. 2013 Jul;191(1):55-62. doi: 10.1016/j.jviromet.2013.03.028. Epub 2013 Apr 9.


Developing country applications of molecular farming: case studies in South Africa and Argentina.

Rybicki EP, Hitzeroth II, Meyers A, Dus Santos MJ, Wigdorovitz A.

Curr Pharm Des. 2013;19(31):5612-21. Review.


Next-generation sequencing of cervical DNA detects human papillomavirus types not detected by commercial kits.

Meiring TL, Salimo AT, Coetzee B, Maree HJ, Moodley J, Hitzeroth II, Freeborough MJ, Rybicki EP, Williamson AL.

Virol J. 2012 Aug 16;9:164. doi: 10.1186/1743-422X-9-164.


Setting up a platform for plant-based influenza virus vaccine production in South Africa.

Mortimer E, Maclean JM, Mbewana S, Buys A, Williamson AL, Hitzeroth II, Rybicki EP.

BMC Biotechnol. 2012 Apr 26;12:14. doi: 10.1186/1472-6750-12-14.


Vaccine farming in Cape Town.

Rybicki EP, Williamson AL, Meyers A, Hitzeroth II.

Hum Vaccin. 2011 Mar;7(3):339-48. Epub 2011 Mar 1. Review.


Global genetic diversity and geographical and host-species distribution of beak and feather disease virus isolates.

Varsani A, Regnard GL, Bragg R, Hitzeroth II, Rybicki EP.

J Gen Virol. 2011 Apr;92(Pt 4):752-67. doi: 10.1099/vir.0.028126-0. Epub 2010 Dec 22.


A unique isolate of beak and feather disease virus isolated from budgerigars (Melopsittacus undulatus) in South Africa.

Varsani A, de Villiers GK, Regnard GL, Bragg RR, Kondiah K, Hitzeroth II, Rybicki EP.

Arch Virol. 2010 Mar;155(3):435-9. doi: 10.1007/s00705-010-0589-0. Epub 2010 Feb 3.


High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector.

Regnard GL, Halley-Stott RP, Tanzer FL, Hitzeroth II, Rybicki EP.

Plant Biotechnol J. 2010 Jan;8(1):38-46. doi: 10.1111/j.1467-7652.2009.00462.x. Epub 2009 Nov 19.


Immunogenicity of an HPV-16 L2 DNA vaccine.

Hitzeroth II, Passmore JA, Shephard E, Stewart D, Müller M, Williamson AL, Rybicki EP, Kast WM.

Vaccine. 2009 Oct 30;27(46):6432-4. doi: 10.1016/j.vaccine.2009.06.015. Epub 2009 Jun 24.


Insights into the role and function of L2, the minor capsid protein of papillomaviruses.

Pereira R, Hitzeroth II, Rybicki EP.

Arch Virol. 2009;154(2):187-97. doi: 10.1007/s00705-009-0310-3. Epub 2009 Jan 25. Review.


Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum.

Kohl TO, Hitzeroth II, Christensen ND, Rybicki EP.

BMC Biotechnol. 2007 Sep 12;7:56.


Optimization of human papillomavirus type 16 (HPV-16) L1 expression in plants: comparison of the suitability of different HPV-16 L1 gene variants and different cell-compartment localization.

Maclean J, Koekemoer M, Olivier AJ, Stewart D, Hitzeroth II, Rademacher T, Fischer R, Williamson AL, Rybicki EP.

J Gen Virol. 2007 May;88(Pt 5):1460-9.


Comparison of cervical and blood T-cell responses to human papillomavirus-16 in women with human papillomavirus-associated cervical intraepithelial neoplasia.

Passmore JA, Milner M, Denny L, Sampson C, Marais DJ, Allan B, Gumbi PP, Hitzeroth II, Rybicki EP, Williamson AL.

Immunology. 2006 Dec;119(4):507-14. Epub 2006 Oct 9.


Plant-produced cottontail rabbit papillomavirus L1 protein protects against tumor challenge: a proof-of-concept study.

Kohl T, Hitzeroth II, Stewart D, Varsani A, Govan VA, Christensen ND, Williamson AL, Rybicki EP.

Clin Vaccine Immunol. 2006 Aug;13(8):845-53.

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