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Items: 35


Transcriptional Terminators Allow Leak-Free Chromosomal Integration of Genetic Constructs in Cyanobacteria.

Kelly CL, Taylor GM, Šatkutė A, Dekker L, Heap JT.

Microorganisms. 2019 Aug 16;7(8). pii: E263. doi: 10.3390/microorganisms7080263.


Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering.

Taylor GM, Mordaka PM, Heap JT.

Nucleic Acids Res. 2019 Feb 20;47(3):e17. doi: 10.1093/nar/gky1182.


Synthetic negative feedback circuits using engineered small RNAs.

Kelly CL, Harris AWK, Steel H, Hancock EJ, Heap JT, Papachristodoulou A.

Nucleic Acids Res. 2018 Oct 12;46(18):9875-9889. doi: 10.1093/nar/gky828.


The Butanol Producing Microbe Clostridium beijerinckii NCIMB 14988 Manipulated Using Forward and Reverse Genetic Tools.

Little GT, Willson BJ, Heap JT, Winzer K, Minton NP.

Biotechnol J. 2018 Nov;13(11):e1700711. doi: 10.1002/biot.201700711. Epub 2018 Apr 29.


A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria.

Kelly CL, Taylor GM, Hitchcock A, Torres-Méndez A, Heap JT.

ACS Synth Biol. 2018 Apr 20;7(4):1056-1066. doi: 10.1021/acssynbio.7b00435. Epub 2018 Apr 2.


Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates.

Mordaka PM, Heap JT.

ACS Synth Biol. 2018 Feb 16;7(2):672-681. doi: 10.1021/acssynbio.7b00398. Epub 2018 Jan 26.


Inactivation of the dnaK gene in Clostridium difficile 630 Δerm yields a temperature-sensitive phenotype and increases biofilm-forming ability.

Jain S, Smyth D, O'Hagan BMG, Heap JT, McMullan G, Minton NP, Ternan NG.

Sci Rep. 2017 Dec 13;7(1):17522. doi: 10.1038/s41598-017-17583-9.


Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application.

Sellés Vidal L, Kelly CL, Mordaka PM, Heap JT.

Biochim Biophys Acta Proteins Proteom. 2018 Feb;1866(2):327-347. doi: 10.1016/j.bbapap.2017.11.005. Epub 2017 Nov 10. Review.


In Vitro Susceptibility to Closthioamide among Clinical and Reference Strains of Neisseria gonorrhoeae.

Miari VF, Solanki P, Hleba Y, Stabler RA, Heap JT.

Antimicrob Agents Chemother. 2017 Sep 22;61(10). pii: e00929-17. doi: 10.1128/AAC.00929-17. Print 2017 Oct.


A genetic assay for gene essentiality in Clostridium.

Walker DJF, Heap JT, Winzer K, Minton NP.

Anaerobe. 2016 Dec;42:40-43. doi: 10.1016/j.anaerobe.2016.07.007. Epub 2016 Jul 31.


Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter.

Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, Estévez A, Kato A, Marqvorsen MH, Fleet GW, Estévez RJ, Izumori K, Heap JT.

ACS Synth Biol. 2016 Oct 21;5(10):1136-1145. Epub 2016 Jun 21.


6-Deoxyhexoses from l-Rhamnose in the Search for Inducers of the Rhamnose Operon: Synergy of Chemistry and Biotechnology.

Liu Z, Yoshihara A, Kelly C, Heap JT, Marqvorsen MH, Jenkinson SF, Wormald MR, Otero JM, Estévez A, Kato A, Fleet GW, Estévez RJ, Izumori K.

Chemistry. 2016 Aug 22;22(35):12557-65. doi: 10.1002/chem.201602482. Epub 2016 Jul 21.


Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.

Ehsaan M, Kuit W, Zhang Y, Cartman ST, Heap JT, Winzer K, Minton NP.

Biotechnol Biofuels. 2016 Jan 4;9:4. doi: 10.1186/s13068-015-0410-0. eCollection 2016.


Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo.

Heap JT, Theys J, Ehsaan M, Kubiak AM, Dubois L, Paesmans K, Van Mellaert L, Knox R, Kuehne SA, Lambin P, Minton NP.

Oncotarget. 2014 Apr 15;5(7):1761-9.


Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.

Kovács K, Willson BJ, Schwarz K, Heap JT, Jackson A, Bolam DN, Winzer K, Minton NP.

Biotechnol Biofuels. 2013 Aug 20;6(1):117. doi: 10.1186/1754-6834-6-117.


Two-component signal transduction system CBO0787/CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502.

Zhang Z, Korkeala H, Dahlsten E, Sahala E, Heap JT, Minton NP, Lindström M.

PLoS Pathog. 2013 Mar;9(3):e1003252. doi: 10.1371/journal.ppat.1003252. Epub 2013 Mar 28.


The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum.

Jabbari S, Steiner E, Heap JT, Winzer K, Minton NP, King JR.

Math Biosci. 2013 Jun;243(2):223-39. doi: 10.1016/j.mbs.2013.03.005. Epub 2013 Mar 26.


Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures.

Lindström M, Dahlsten E, Söderholm H, Selby K, Somervuo P, Heap JT, Minton NP, Korkeala H.

Appl Environ Microbiol. 2012 Aug;78(15):5466-70. doi: 10.1128/AEM.00555-12. Epub 2012 Jun 1.


Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production.

Cartman ST, Kelly ML, Heeg D, Heap JT, Minton NP.

Appl Environ Microbiol. 2012 Jul;78(13):4683-90. doi: 10.1128/AEM.00249-12. Epub 2012 Apr 20.


Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis.

Saad NY, Schiel B, Brayé M, Heap JT, Minton NP, Dürre P, Becker HD.

J Biol Chem. 2012 Jun 8;287(24):20382-94. doi: 10.1074/jbc.M111.332304. Epub 2012 Apr 13.


Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton NP.

Nucleic Acids Res. 2012 Apr;40(8):e59. doi: 10.1093/nar/gkr1321. Epub 2012 Jan 18.


ClosTron-mediated engineering of Clostridium.

Kuehne SA, Heap JT, Cooksley CM, Cartman ST, Minton NP.

Methods Mol Biol. 2011;765:389-407. doi: 10.1007/978-1-61779-197-0_23.


Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum.

Steiner E, Dago AE, Young DI, Heap JT, Minton NP, Hoch JA, Young M.

Mol Microbiol. 2011 May;80(3):641-54. doi: 10.1111/j.1365-2958.2011.07608.x. Epub 2011 Mar 14.


Important role of class I heat shock genes hrcA and dnaK in the heat shock response and the response to pH and NaCl stress of group I Clostridium botulinum strain ATCC 3502.

Selby K, Lindström M, Somervuo P, Heap JT, Minton NP, Korkeala H.

Appl Environ Microbiol. 2011 May;77(9):2823-30. doi: 10.1128/AEM.02633-10. Epub 2011 Mar 4.


The diverse sporulation characteristics of Clostridium difficile clinical isolates are not associated with type.

Burns DA, Heap JT, Minton NP.

Anaerobe. 2010 Dec;16(6):618-22. doi: 10.1016/j.anaerobe.2010.10.001. Epub 2010 Oct 13.


Clostridium difficile spore germination: an update.

Burns DA, Heap JT, Minton NP.

Res Microbiol. 2010 Nov;161(9):730-4. doi: 10.1016/j.resmic.2010.09.007. Epub 2010 Sep 21. Review.


The role of toxin A and toxin B in Clostridium difficile infection.

Kuehne SA, Cartman ST, Heap JT, Kelly ML, Cockayne A, Minton NP.

Nature. 2010 Oct 7;467(7316):711-3. doi: 10.1038/nature09397. Epub 2010 Sep 15.


ClosTron-targeted mutagenesis.

Heap JT, Cartman ST, Kuehne SA, Cooksley C, Minton NP.

Methods Mol Biol. 2010;646:165-82. doi: 10.1007/978-1-60327-365-7_11.


The emergence of 'hypervirulence' in Clostridium difficile.

Cartman ST, Heap JT, Kuehne SA, Cockayne A, Minton NP.

Int J Med Microbiol. 2010 Aug;300(6):387-95. doi: 10.1016/j.ijmm.2010.04.008. Epub 2010 May 23. Review.


Mathematical modelling of the sporulation-initiation network in Bacillus subtilis revealing the dual role of the putative quorum-sensing signal molecule PhrA.

Jabbari S, Heap JT, King JR.

Bull Math Biol. 2011 Jan;73(1):181-211. doi: 10.1007/s11538-010-9530-7. Epub 2010 Mar 18.


SleC is essential for germination of Clostridium difficile spores in nutrient-rich medium supplemented with the bile salt taurocholate.

Burns DA, Heap JT, Minton NP.

J Bacteriol. 2010 Feb;192(3):657-64. doi: 10.1128/JB.01209-09. Epub 2009 Nov 20.


Construction of a nontoxigenic Clostridium botulinum strain for food challenge studies.

Bradshaw M, Marshall KM, Heap JT, Tepp WH, Minton NP, Johnson EA.

Appl Environ Microbiol. 2010 Jan;76(2):387-93. doi: 10.1128/AEM.02005-09. Epub 2009 Nov 20.


The ClosTron: Mutagenesis in Clostridium refined and streamlined.

Heap JT, Kuehne SA, Ehsaan M, Cartman ST, Cooksley CM, Scott JC, Minton NP.

J Microbiol Methods. 2010 Jan;80(1):49-55. doi: 10.1016/j.mimet.2009.10.018. Epub 2009 Nov 3.


A modular system for Clostridium shuttle plasmids.

Heap JT, Pennington OJ, Cartman ST, Minton NP.

J Microbiol Methods. 2009 Jul;78(1):79-85. doi: 10.1016/j.mimet.2009.05.004. Epub 2009 May 13.


The ClosTron: a universal gene knock-out system for the genus Clostridium.

Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP.

J Microbiol Methods. 2007 Sep;70(3):452-64. Epub 2007 Jun 18.


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