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Items: 1 to 50 of 66

1.

Data Acceptance Criteria for Standardized Human-Associated Fecal Source Identification Quantitative Real-Time PCR Methods.

Shanks OC, Kelty CA, Oshiro R, Haugland RA, Madi T, Brooks L, Field KG, Sivaganesan M.

Appl Environ Microbiol. 2016 Apr 18;82(9):2773-2782. doi: 10.1128/AEM.03661-15. Print 2016 May.

2.

Multi-laboratory survey of qPCR enterococci analysis method performance in U.S. coastal and inland surface waters.

Haugland RA, Siefring S, Varma M, Oshima KH, Sivaganesan M, Cao Y, Raith M, Griffith J, Weisberg SB, Noble RT, Blackwood AD, Kinzelman J, Anan'eva T, Bushon RN, Stelzer EA, Harwood VJ, Gordon KV, Sinigalliano C.

J Microbiol Methods. 2016 Apr;123:114-25. doi: 10.1016/j.mimet.2016.01.017. Epub 2016 Feb 2.

PMID:
26844886
3.

Development and evaluation of a culture-independent method for source determination of fecal wastes in surface and storm waters using reverse transcriptase-PCR detection of FRNA coliphage genogroup gene sequences.

Paar J 3rd, Doolittle MM, Varma M, Siefring S, Oshima K, Haugland RA.

J Microbiol Methods. 2015 May;112:28-35. doi: 10.1016/j.mimet.2015.02.015. Epub 2015 Mar 3.

PMID:
25744574
4.

Influence of wastewater disinfection on densities of culturable fecal indicator bacteria and genetic markers.

Chern EC, Brenner K, Wymer L, Haugland RA.

J Water Health. 2014 Sep;12(3):410-7. doi: 10.2166/wh.2013.179.

PMID:
25252344
5.

Water quality, weather and environmental factors associated with fecal indicator organism density in beach sand at two recreational marine beaches.

Heaney CD, Exum NG, Dufour AP, Brenner KP, Haugland RA, Chern E, Schwab KJ, Love DC, Serre ML, Noble R, Wade TJ.

Sci Total Environ. 2014 Nov 1;497-498:440-447. doi: 10.1016/j.scitotenv.2014.07.113. Epub 2014 Aug 20.

6.

Standardization of enterococci density estimates by EPA qPCR methods and comparison of beach action value exceedances in river waters with culture methods.

Haugland RA, Siefring SD, Varma M, Dufour AP, Brenner KP, Wade TJ, Sams E, Cochran S, Braun S, Sivaganensan M.

J Microbiol Methods. 2014 Oct;105:59-66. doi: 10.1016/j.mimet.2014.07.007. Epub 2014 Jul 16.

PMID:
25038459
7.

Comparison of Enterococcus quantitative polymerase chain reaction analysis results from Midwest U.S. river samples using EPA Method 1611 and Method 1609 PCR reagents.

Sivaganesan M, Siefring S, Varma M, Haugland RA.

J Microbiol Methods. 2014 Jun;101:9-17. doi: 10.1016/j.mimet.2014.03.004. Epub 2014 Mar 26. Erratum in: J Microbiol Methods. 2015 Aug;115:166. Sivaganensan, Mano [corrected to Sivaganesan, Mano].

PMID:
24681207
8.

Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples.

Green HC, Haugland RA, Varma M, Millen HT, Borchardt MA, Field KG, Walters WA, Knight R, Sivaganesan M, Kelty CA, Shanks OC.

Appl Environ Microbiol. 2014 May;80(10):3086-94. doi: 10.1128/AEM.04137-13. Epub 2014 Mar 7.

9.

Human fecal source identification with real-time quantitative PCR.

Shanks OC, Peed L, Sivaganesan M, Haugland RA, Chern EC.

Methods Mol Biol. 2014;1096:85-99. doi: 10.1007/978-1-62703-712-9_7.

PMID:
24515362
10.

Effect of platform, reference material, and quantification model on enumeration of Enterococcus by quantitative PCR methods.

Cao Y, Sivaganesan M, Kinzelman J, Blackwood AD, Noble RT, Haugland RA, Griffith JF, Weisberg SB.

Water Res. 2013 Jan 1;47(1):233-41. doi: 10.1016/j.watres.2012.09.056. Epub 2012 Oct 22.

PMID:
23123048
11.

Influences of sample interference and interference controls on quantification of enterococci fecal indicator bacteria in surface water samples by the qPCR method.

Haugland RA, Siefring S, Lavender J, Varma M.

Water Res. 2012 Nov 15;46(18):5989-6001. doi: 10.1016/j.watres.2012.08.017. Epub 2012 Aug 27.

PMID:
22981586
12.

Dramatic improvements in beach water quality following gull removal.

Converse RR, Kinzelman JL, Sams EA, Hudgens E, Dufour AP, Ryu H, Santo-Domingo JW, Kelty CA, Shanks OC, Siefring SD, Haugland RA, Wade TJ.

Environ Sci Technol. 2012 Sep 18;46(18):10206-13. doi: 10.1021/es302306b. Epub 2012 Sep 5.

PMID:
22913457
13.

Comparison of Enterococcus quantitative polymerase chain reaction analysis results from fresh and marine waters on two real-time instruments.

Sivaganensan M, Varma M, Haugland RA.

Anal Biochem. 2012 Nov 1;430(1):68-74. doi: 10.1016/j.ab.2012.07.020. Epub 2012 Aug 1.

PMID:
22863982
14.

Distribution of genetic marker concentrations for fecal indicator bacteria in sewage and animal feces.

Kelty CA, Varma M, Sivaganesan M, Haugland RA, Shanks OC.

Appl Environ Microbiol. 2012 Jun;78(12):4225-32. doi: 10.1128/AEM.07819-11. Epub 2012 Apr 13.

15.

Correlation between quantitative PCR and culture-based methods for measuring Enterococcus spp. over various temporal scales at three California marine beaches.

Converse RR, Griffith JF, Noble RT, Haugland RA, Schiff KC, Weisberg SB.

Appl Environ Microbiol. 2012 Feb;78(4):1237-42. doi: 10.1128/AEM.07136-11. Epub 2011 Dec 16.

16.

Fecal indicators in sand, sand contact, and risk of enteric illness among beachgoers.

Heaney CD, Sams E, Dufour AP, Brenner KP, Haugland RA, Chern E, Wing S, Marshall S, Love DC, Serre M, Noble R, Wade TJ.

Epidemiology. 2012 Jan;23(1):95-106. doi: 10.1097/EDE.0b013e31823b504c.

17.

Interlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria.

Shanks OC, Sivaganesan M, Peed L, Kelty CA, Blackwood AD, Greene MR, Noble RT, Bushon RN, Stelzer EA, Kinzelman J, Anan'eva T, Sinigalliano C, Wanless D, Griffith J, Cao Y, Weisberg S, Harwood VJ, Staley C, Oshima KH, Varma M, Haugland RA.

Environ Sci Technol. 2012 Jan 17;46(2):945-53. doi: 10.1021/es2031455. Epub 2011 Dec 16. Erratum in: Environ Sci Technol. 2012 Feb 21;46(4):2478.

PMID:
22133009
18.

MPN estimation of qPCR target sequence recoveries from whole cell calibrator samples.

Sivaganesan M, Siefring S, Varma M, Haugland RA.

J Microbiol Methods. 2011 Dec;87(3):343-9. doi: 10.1016/j.mimet.2011.09.013. Epub 2011 Oct 1.

PMID:
21989298
19.

Differential decay of human faecal Bacteroides in marine and freshwater.

Green HC, Shanks OC, Sivaganesan M, Haugland RA, Field KG.

Environ Microbiol. 2011 Dec;13(12):3235-49. doi: 10.1111/j.1462-2920.2011.02549.x. Epub 2011 Sep 1.

PMID:
21883797
20.

Comparison of quantitative PCR assays for Escherichia coli targeting ribosomal RNA and single copy genes.

Chern EC, Siefring S, Paar J, Doolittle M, Haugland RA.

Lett Appl Microbiol. 2011 Mar;52(3):298-306. doi: 10.1111/j.1472-765X.2010.03001.x. Epub 2011 Jan 19.

21.

Performance of PCR-based assays targeting Bacteroidales genetic markers of human fecal pollution in sewage and fecal samples.

Shanks OC, White K, Kelty CA, Sivaganesan M, Blannon J, Meckes M, Varma M, Haugland RA.

Environ Sci Technol. 2010 Aug 15;44(16):6281-8. doi: 10.1021/es100311n.

PMID:
20704227
22.

Improved strategies and optimization of calibration models for real-time PCR absolute quantification.

Sivaganesan M, Haugland RA, Chern EC, Shanks OC.

Water Res. 2010 Sep;44(16):4726-35. doi: 10.1016/j.watres.2010.07.066. Epub 2010 Jul 29.

PMID:
20701947
23.

Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR.

Haugland RA, Varma M, Sivaganesan M, Kelty C, Peed L, Shanks OC.

Syst Appl Microbiol. 2010 Oct;33(6):348-57. doi: 10.1016/j.syapm.2010.06.001. Epub 2010 Jul 24.

PMID:
20655680
24.

Relationship and variation of qPCR and culturable Enterococci estimates in ambient surface waters are predictable.

Whitman RL, Ge Z, Nevers MB, Boehm AB, Chern EC, Haugland RA, Lukasik AM, Molina M, Przybyla-Kelly K, Shively DA, White EM, Zepp RG, Byappanahalli MN.

Environ Sci Technol. 2010 Jul 1;44(13):5049-54. doi: 10.1021/es9028974.

PMID:
20527919
25.

Performance assessment PCR-based assays targeting bacteroidales genetic markers of bovine fecal pollution.

Shanks OC, White K, Kelty CA, Hayes S, Sivaganesan M, Jenkins M, Varma M, Haugland RA.

Appl Environ Microbiol. 2010 Mar;76(5):1359-66. doi: 10.1128/AEM.02033-09. Epub 2010 Jan 8.

26.

Quantitative PCR for genetic markers of human fecal pollution.

Shanks OC, Kelty CA, Sivaganesan M, Varma M, Haugland RA.

Appl Environ Microbiol. 2009 Sep;75(17):5507-13. doi: 10.1128/AEM.00305-09. Epub 2009 Jul 10.

27.

A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards.

Sivaganesan M, Seifring S, Varma M, Haugland RA, Shanks OC.

BMC Bioinformatics. 2008 Feb 25;9:120. doi: 10.1186/1471-2105-9-120.

28.

Improved real-time PCR assays for the detection of fecal indicator bacteria in surface waters with different instrument and reagent systems.

Siefring S, Varma M, Atikovic E, Wymer L, Haugland RA.

J Water Health. 2008 Jun;6(2):225-37. doi: 10.2166/wh.2008.022.

PMID:
18209285
29.

Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution.

Shanks OC, Atikovic E, Blackwood AD, Lu J, Noble RT, Domingo JS, Seifring S, Sivaganesan M, Haugland RA.

Appl Environ Microbiol. 2008 Feb;74(3):745-52. Epub 2007 Dec 7.

30.

Opportunistic Aspergillus pathogens measured in home and hospital tap water by quantitative PCR (QPCR).

Vesper SJ, Haugland RA, Rogers ME, Neely AN.

J Water Health. 2007 Sep;5(3):427-31.

PMID:
17878557
31.
32.

Comparison of mold concentrations quantified by MSQPCR in indoor and outdoor air sampled simultaneously.

Meklin T, Reponen T, McKinstry C, Cho SH, Grinshpun SA, Nevalainen A, Vepsäläinen A, Haugland RA, Lemasters G, Vesper SJ.

Sci Total Environ. 2007 Aug 15;382(1):130-4. Epub 2007 Apr 30.

33.

Relative moldiness index as predictor of childhood respiratory illness.

Vesper SJ, McKinstry C, Haugland RA, Iossifova Y, Lemasters G, Levin L, Khurana Hershey GK, Villareal M, Bernstein DI, Lockey J, Reponen T.

J Expo Sci Environ Epidemiol. 2007 Jan;17(1):88-94. Epub 2006 Oct 11.

34.

Specific molds associated with asthma in water-damaged homes.

Vesper SJ, McKinstry C, Yang C, Haugland RA, Kercsmar CM, Yike I, Schluchter MD, Kirchner HL, Sobolewski J, Allan TM, Dearborn DG.

J Occup Environ Med. 2006 Aug;48(8):852-8.

PMID:
16902378
35.

Confirmational Identification of Escherichia coli, a Comparison of Genotypic and Phenotypic Assays for Glutamate Decarboxylase and beta-d-Glucuronidase.

McDaniels AE, Rice EW, Reyes AL, Johnson CH, Haugland RA, Stelma GN.

Appl Environ Microbiol. 1998 Oct;64(10):4113.

36.

Comparison of populations of mould species in homes in the UK and USA using mould-specific quantitative PCR.

Vesper SJ, Wymer LJ, Meklin T, Varma M, Stott R, Richardson M, Haugland RA.

Lett Appl Microbiol. 2005;41(4):367-73.

37.

Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis.

Haugland RA, Siefring SC, Wymer LJ, Brenner KP, Dufour AP.

Water Res. 2005 Feb;39(4):559-68. Epub 2004 Dec 24.

PMID:
15707628
38.

Quantitative PCR analysis of house dust can reveal abnormal mold conditions.

Meklin T, Haugland RA, Reponen T, Varma M, Lummus Z, Bernstein D, Wymer LJ, Vesper SJ.

J Environ Monit. 2004 Jul;6(7):615-20. Epub 2004 May 26.

39.

Quantitative polymerase chain reaction analysis of fungi in dust from homes of infants who developed idiopathic pulmonary hemorrhaging.

Vesper SJ, Varma M, Wymer LJ, Dearborn DG, Sobolewski J, Haugland RA.

J Occup Environ Med. 2004 Jun;46(6):596-601.

PMID:
15213523
40.

Monitoring Aspergillus species by quantitative PCR during construction of a multi-storey hospital building.

Morrison J, Yang C, Lin KT, Haugland RA, Neely AN, Vesper SJ.

J Hosp Infect. 2004 May;57(1):85-7.

PMID:
15142721
41.

Rapid monitoring by quantitative polymerase chain reaction for pathogenic Aspergillus during carpet removal from a hospital.

Neely AN, Gallardo V, Barth E, Haugland RA, Warden GD, Vesper SJ.

Infect Control Hosp Epidemiol. 2004 Apr;25(4):350-2.

PMID:
15108736
42.

Quantitative PCR analysis of selected Aspergillus, Penicillium and Paecilomyces species.

Haugland RA, Varma M, Wymer LJ, Vesper SJ.

Syst Appl Microbiol. 2004 Mar;27(2):198-210.

PMID:
15046309
43.

Real-time PCR method to detect Enterococcus faecalis in water.

Santo Domingo JW, Siefring SC, Haugland RA.

Biotechnol Lett. 2003 Feb;25(3):261-5.

PMID:
12882582
44.

Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water.

Brinkman NE, Haugland RA, Wymer LJ, Byappanahalli M, Whitman RL, Vesper SJ.

Appl Environ Microbiol. 2003 Mar;69(3):1775-82.

45.

Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis.

Haugland RA, Brinkman N, Vesper SJ.

J Microbiol Methods. 2002 Aug;50(3):319-23.

PMID:
12031583
46.

Quantification of Stachybotrys chartarum conidia in indoor dust using real time, fluorescent probe-based detection of PCR products.

Roe JD, Haugland RA, Vesper SJ, Wymer LJ.

J Expo Anal Environ Epidemiol. 2001 Jan-Feb;11(1):12-20.

PMID:
11246797
47.

Initial characterization of the hemolysin stachylysin from Stachybotrys chartarum.

Vesper SJ, Magnuson ML, Dearborn DG, Yike I, Haugland RA.

Infect Immun. 2001 Feb;69(2):912-6.

49.

Evaluation of Stachybotrys chartarum in the house of an infant with pulmonary hemorrhage: quantitative assessment before, during, and after remediation.

Vesper S, Dearborn DG, Yike I, Allan T, Sobolewski J, Hinkley SF, Jarvis BB, Haugland RA.

J Urban Health. 2000 Mar;77(1):68-85.

50.

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