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J Clin Microbiol. 2013 Dec;51(12):4073-8. doi: 10.1128/JCM.01924-13. Epub 2013 Oct 2.

wzi Gene sequencing, a rapid method for determination of capsular type for Klebsiella strains.

Author information

1
Institut Pasteur, Microbial Evolutionary Genomics, Paris, France.

Abstract

Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing of Klebsiella species still has important applications in epidemiology and clinical microbiology, but the serological method has strong practical limitations. Our objective was to evaluate the sequencing of wzi, a gene conserved in all capsular types of Klebsiella pneumoniae that codes for an outer membrane protein involved in capsule attachment to the cell surface, as a simple and rapid method for the prediction of K type. The sequencing of a 447-nucleotide region of wzi distinguished the K-type reference strains with only nine exceptions. A reference wzi sequence database was created by the inclusion of multiple strains representing K types associated with high virulence and multidrug resistance. A collection of 119 prospective clinical isolates of K. pneumoniae were then analyzed in parallel by wzi sequencing and classical K typing. Whereas K typing achieved typeability for 81% and discrimination for 94.4% of the isolates, these figures were 98.1% and 98.3%, respectively, for wzi sequencing. The prediction of K type once the wzi allele was known was 94%. wzi sequencing is a rapid and simple method for the determination of the K types of most K. pneumoniae clinical isolates.

PMID:
24088853
PMCID:
PMC3838100
DOI:
10.1128/JCM.01924-13
[Indexed for MEDLINE]
Free PMC Article

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