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RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR.

Zhang J, Harmon KM.

Methods Mol Biol. 2020;2123:295-310. doi: 10.1007/978-1-0716-0346-8_21.


Detection of live attenuated influenza vaccine virus and evidence of reassortment in the U.S. swine population.

Sharma A, Zeller MA, Li G, Harmon KM, Zhang J, Hoang H, Anderson TK, Vincent AL, Gauger PC.

J Vet Diagn Invest. 2020 Mar;32(2):301-311. doi: 10.1177/1040638720907918. Epub 2020 Feb 26.


Emergence of Salmonella enterica serovar 4,[5],12:i:- as the primary serovar identified from swine clinical samples and development of a multiplex real-time PCR for improved Salmonella serovar-level identification.

Naberhaus SA, Krull AC, Bradner LK, Harmon KM, Arruda P, Arruda BL, Sahin O, Burrough ER, Schwartz KJ, Kreuder AJ.

J Vet Diagn Invest. 2019 Nov;31(6):818-827. doi: 10.1177/1040638719883843. Epub 2019 Oct 24.


Polioencephalomyelitis in Domestic Swine Associated With Porcine Astrovirus Type 3.

Matias Ferreyra FS, Bradner LK, Burrough ER, Cooper VL, Derscheid RJ, Gauger PC, Harmon KM, Madson D, Piñeyro PE, Schwartz KJ, Stevenson GW, Zeller MA, Arruda BL.

Vet Pathol. 2020 Jan;57(1):82-89. doi: 10.1177/0300985819875741. Epub 2019 Sep 24.


Detection, isolation, and in vitro characterization of porcine parainfluenza virus type 1 isolated from respiratory diagnostic specimens in swine.

Park JY, Welch MW, Harmon KM, Zhang J, Piñeyro PE, Li G, Hause BM, Gauger PC.

Vet Microbiol. 2019 Jan;228:219-225. doi: 10.1016/j.vetmic.2018.12.002. Epub 2018 Dec 5.


Complete Genome Sequences of Two Novel Human-Like H3N2 Influenza A Viruses, A/swine/Oklahoma/65980/2017 (H3N2) and A/Swine/Oklahoma/65260/2017 (H3N2), Detected in Swine in the United States.

Zeller MA, Li G, Harmon KM, Zhang J, Vincent AL, Anderson TK, Gauger PC.

Microbiol Resour Announc. 2018 Nov 21;7(20). pii: e01203-18. doi: 10.1128/MRA.01203-18. eCollection 2018 Nov.


Detection and genomic characterization of new avian-like hepatitis E virus in a sparrow in the United States.

Yang C, Wang L, Shen H, Zheng Y, Gauger PC, Chen Q, Zhang J, Yoon KJ, Harmon KM, Main RG, Li G.

Arch Virol. 2018 Oct;163(10):2861-2864. doi: 10.1007/s00705-018-3925-4. Epub 2018 Jul 3.


The emergence of novel sparrow deltacoronaviruses in the United States more closely related to porcine deltacoronaviruses than sparrow deltacoronavirus HKU17.

Chen Q, Wang L, Yang C, Zheng Y, Gauger PC, Anderson T, Harmon KM, Zhang J, Yoon KJ, Main RG, Li G.

Emerg Microbes Infect. 2018 Jun 6;7(1):105. doi: 10.1038/s41426-018-0108-z. No abstract available.


Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States.

Chen Q, Wang L, Zheng Y, Zhang J, Guo B, Yoon KJ, Gauger PC, Harmon KM, Main RG, Li G.

Virol J. 2018 May 25;15(1):95. doi: 10.1186/s12985-018-1001-z.


Detection and genetic characterization of porcine pegivirus in pigs in the United States.

Yang C, Wang L, Shen H, Zheng Y, Bade SA, Gauger PC, Chen Q, Zhang J, Guo B, Yoon KJ, Harmon KM, Main RG, Li G.

Transbound Emerg Dis. 2018 Jun;65(3):618-626. doi: 10.1111/tbed.12844. Epub 2018 Feb 27.


Detection of porcine reproductive and respiratory syndrome virus (PRRSV)-specific IgM-IgA in oral fluid samples reveals PRRSV infection in the presence of maternal antibody.

Rotolo ML, Giménez-Lirola L, Ji J, Magtoto R, Henao-Díaz YA, Wang C, Baum DH, Harmon KM, Main RG, Zimmerman JJ.

Vet Microbiol. 2018 Feb;214:13-20. doi: 10.1016/j.vetmic.2017.11.011. Epub 2017 Nov 17.


Risk of exposure to Coxiella burnetii from ruminant livestock exhibited at Iowa agricultural fairs.

Still Brooks KM, Stensland WR, Harmon KM, O'Connor AM, Plummer PJ.

Zoonoses Public Health. 2018 May;65(3):334-338. doi: 10.1111/zph.12439. Epub 2017 Dec 17.


Porcine reproductive and respiratory disease virus: Evolution and recombination yields distinct ORF5 RFLP 1-7-4 viruses with individual pathogenicity.

van Geelen AGM, Anderson TK, Lager KM, Das PB, Otis NJ, Montiel NA, Miller LC, Kulshreshtha V, Buckley AC, Brockmeier SL, Zhang J, Gauger PC, Harmon KM, Faaberg KS.

Virology. 2018 Jan 1;513:168-179. doi: 10.1016/j.virol.2017.10.002. Epub 2017 Nov 5.


Complete Genome Sequence of Porcine respirovirus 1 Strain USA/MN25890NS/2016, Isolated in the United States.

Park JY, Welch M, Harmon KM, Zhang J, Piñeyro PE, Li G, Gauger PC.

Genome Announc. 2017 Oct 19;5(42). pii: e01139-17. doi: 10.1128/genomeA.01139-17.


Detection and characterization of an H4N6 avian-lineage influenza A virus in pigs in the Midwestern United States.

Abente EJ, Gauger PC, Walia RR, Rajao DS, Zhang J, Harmon KM, Killian ML, Vincent AL.

Virology. 2017 Nov;511:56-65. doi: 10.1016/j.virol.2017.08.021. Epub 2017 Aug 23.


Sampling guidelines for oral fluid-based surveys of group-housed animals.

Rotolo ML, Sun Y, Wang C, Giménez-Lirola L, Baum DH, Gauger PC, Harmon KM, Hoogland M, Main R, Zimmerman JJ.

Vet Microbiol. 2017 Sep;209:20-29. doi: 10.1016/j.vetmic.2017.02.004. Epub 2017 Feb 17.


High-throughput whole genome sequencing of Porcine reproductive and respiratory syndrome virus from cell culture materials and clinical specimens using next-generation sequencing technology.

Zhang J, Zheng Y, Xia XQ, Chen Q, Bade SA, Yoon KJ, Harmon KM, Gauger PC, Main RG, Li G.

J Vet Diagn Invest. 2017 Jan;29(1):41-50. doi: 10.1177/1040638716673404. Epub 2016 Dec 20.


PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014-2016.

Xiao CT, Harmon KM, Halbur PG, Opriessnig T.

Vet Microbiol. 2016 Dec 25;197:72-77. doi: 10.1016/j.vetmic.2016.11.009. Epub 2016 Nov 11.


West Nile Virus Infection in Human and Mouse Cornea Tissue.

Blitvich BJ, Wang T, Saxena V, Zeng S, Harmon KM, Raymond MD, Goins KM, Reed CR, Mullins RF, Greiner MA.

Am J Trop Med Hyg. 2016 Nov 2;95(5):1185-1191. Epub 2016 Sep 26.


Complete Genome Sequence of Porcine Sapelovirus Strain USA/IA33375/2015 Identified in the United States.

Chen Q, Zheng Y, Guo B, Zhang J, Yoon KJ, Harmon KM, Main RG, Li G.

Genome Announc. 2016 Sep 29;4(5). pii: e01055-16. doi: 10.1128/genomeA.01055-16.


Outbreak of H5N2 highly pathogenic avian Influenza A virus infection in two commercial layer facilities: lesions and viral antigen distribution.

Arruda PH, Stevenson GW, Killian ML, Burrough ER, Gauger PC, Harmon KM, Magstadt DR, Yoon KJ, Zhang J, Madson DM, Piñeyro P, Derscheid RJ, Schwartz KJ, Cooper VL, Halbur PG, Main RG, Sato Y, Arruda BL.

J Vet Diagn Invest. 2016 Sep;28(5):568-73. doi: 10.1177/1040638716658929. Epub 2016 Jul 16.


Validation of a gauze sponge sampling methodology to detect Tritrichomonas foetus by real-time PCR.

Dewell GA, Phillips PE, Dohlman TM, Harmon KM, Gauger PC.

J Vet Diagn Invest. 2016 Sep;28(5):595-8. doi: 10.1177/1040638716653637. Epub 2016 Jul 7.


Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus.

Zhang J, Tsai YL, Lee PY, Chen Q, Zhang Y, Chiang CJ, Shen YH, Li FC, Chang HF, Gauger PC, Harmon KM, Wang HT.

J Virol Methods. 2016 Aug;234:34-42. doi: 10.1016/j.jviromet.2016.03.016. Epub 2016 Apr 6.


Whole-Genome Sequences of Novel Porcine Circovirus Type 2 Viruses Detected in Swine from Mexico and the United States.

Harmon KM, Gauger PC, Zhang J, Piñeyro PE, Dunn DD, Chriswell AJ.

Genome Announc. 2015 Dec 17;3(6). pii: e01315-15. doi: 10.1128/genomeA.01315-15.


Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs.

Thomas JT, Chen Q, Gauger PC, Giménez-Lirola LG, Sinha A, Harmon KM, Madson DM, Burrough ER, Magstadt DR, Salzbrenner HM, Welch MW, Yoon KJ, Zimmerman JJ, Zhang J.

PLoS One. 2015 Oct 6;10(10):e0139266. doi: 10.1371/journal.pone.0139266. eCollection 2015.


Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs.

Madson DM, Magstadt DR, Arruda PH, Hoang H, Sun D, Bower LP, Bhandari M, Burrough ER, Gauger PC, Pillatzki AE, Stevenson GW, Wilberts BL, Brodie J, Harmon KM, Wang C, Main RG, Zhang J, Yoon KJ.

Vet Microbiol. 2014 Nov 7;174(1-2):60-8. doi: 10.1016/j.vetmic.2014.09.002. Epub 2014 Sep 22.


Reply to "classification of emergent U.S. strains of porcine epidemic diarrhea virus by phylogenetic analysis of nucleocapsid and ORF3 genes".

Zhang J, Chen Q, Gauger PC, Harmon KM, Yoon KJ.

J Clin Microbiol. 2014 Sep;52(9):3511-4. doi: 10.1128/JCM.01747-14. No abstract available.


RNA extraction from swine samples and detection of influenza A virus in swine by real-time RT-PCR.

Zhang J, Harmon KM.

Methods Mol Biol. 2014;1161:277-93. doi: 10.1007/978-1-4939-0758-8_23.


Full-Length Genome Sequence of Porcine Deltacoronavirus Strain USA/IA/2014/8734.

Li G, Chen Q, Harmon KM, Yoon KJ, Schwartz KJ, Hoogland MJ, Gauger PC, Main RG, Zhang J.

Genome Announc. 2014 Apr 10;2(2). pii: e00278-14. doi: 10.1128/genomeA.00278-14.


Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States.

Chen Q, Li G, Stasko J, Thomas JT, Stensland WR, Pillatzki AE, Gauger PC, Schwartz KJ, Madson D, Yoon KJ, Stevenson GW, Burrough ER, Harmon KM, Main RG, Zhang J.

J Clin Microbiol. 2014 Jan;52(1):234-43. doi: 10.1128/JCM.02820-13. Epub 2013 Nov 6.


Detection of Salmonella enteritidis in pooled poultry environmental samples using a serotype-specific real-time-polymerase chain reaction assay.

Adams DR, Stensland WR, Wang CH, O'Connor AM, Trampel DW, Harmon KM, Strait EL, Frana TS.

Avian Dis. 2013 Mar;57(1):22-8.


Probability of detecting Porcine reproductive and respiratory syndrome virus infection using pen-based swine oral fluid specimens as a function of within-pen prevalence.

Olsen C, Wang C, Christopher-Hennings J, Doolittle K, Harmon KM, Abate S, Kittawornrat A, Lizano S, Main R, Nelson EA, Otterson T, Panyasing Y, Rademacher C, Rauh R, Shah R, Zimmerman J.

J Vet Diagn Invest. 2013 May;25(3):328-35. doi: 10.1177/1040638713481471. Epub 2013 Mar 27.


Selective breeding for 50 kHz ultrasonic vocalization emission produces alterations in the ontogeny and regulation of rough-and-tumble play.

Webber ES, Harmon KM, Beckwith TJ, Peña S, Burgdorf J, Panksepp J, Cromwell HC.

Behav Brain Res. 2012 Apr 1;229(1):138-44. doi: 10.1016/j.bbr.2012.01.012. Epub 2012 Jan 13.


Inactivation of feline calicivirus as a surrogate for norovirus on lettuce by electron beam irradiation.

Zhou F, Harmon KM, Yoon KJ, Olson DG, Dickson JS.

J Food Prot. 2011 Sep;74(9):1500-3. doi: 10.4315/0362-028X.JFP-11-121.


The effects of prenatal stress on motivation in the rat pup.

Harmon KM, Greenwald ML, McFarland A, Beckwith T, Cromwell HC.

Stress. 2009 May;12(3):250-8. doi: 10.1080/10253890802367265.


Rats selectively bred for low levels of 50 kHz ultrasonic vocalizations exhibit alterations in early social motivation.

Harmon KM, Cromwell HC, Burgdorf J, Moskal JR, Brudzynski SM, Kroes RA, Panksepp J.

Dev Psychobiol. 2008 May;50(4):322-31. doi: 10.1002/dev.20294.


Influenza virus infection in racing greyhounds.

Yoon KJ, Cooper VL, Schwartz KJ, Harmon KM, Kim WI, Janke BH, Strohbehn J, Butts D, Troutman J.

Emerg Infect Dis. 2005 Dec;11(12):1974-6. No abstract available.


Sources and mechanisms of DPOAE generation: implications for the prediction of auditory sensitivity.

Shaffer LA, Withnell RH, Dhar S, Lilly DJ, Goodman SS, Harmon KM.

Ear Hear. 2003 Oct;24(5):367-79. Review.


Arcobacter cryaerophilus and Arcobacter butzleri isolated from preputial fluid of boars and fattening pigs in Brazil.

de Oliveria SJ, Wesley IV, Baetz AL, Harmon KM, Kader II, de Uzeda M.

J Vet Diagn Invest. 1999 Sep;11(5):462-4. No abstract available.


A case report of sporadic ovine listerial menigoencephalitis in Iowa with an overview of livestock and human cases.

Wesley IV, Larson DJ, Harmon KM, Luchansky JB, Schwartz AR.

J Vet Diagn Invest. 2002 Jul;14(4):314-21.


Evolution of porcine reproductive and respiratory syndrome virus during sequential passages in pigs.

Chang CC, Yoon KJ, Zimmerman JJ, Harmon KM, Dixon PM, Dvorak CM, Murtaugh MP.

J Virol. 2002 May;76(10):4750-63.


Fecal shedding of Campylobacter and Arcobacter spp. in dairy cattle.

Wesley IV, Wells SJ, Harmon KM, Green A, Schroeder-Tucker L, Glover M, Siddique I.

Appl Environ Microbiol. 2000 May;66(5):1994-2000.


Effect of challenge dose and route on porcine reproductive and respiratory syndrome virus (PRRSV) infection in young swine.

Yoon KJ, Zimmerman JJ, Chang CC, Cancel-Tirado S, Harmon KM, McGinley MJ.

Vet Res. 1999 Nov-Dec;30(6):629-38.


Antigenic and genetic variations of the 15 kD nucleocapsid protein of porcine reproductive and respiratory syndrome virus isolates.

Yang L, Yoon KJ, Li Y, Lee JH, Zimmerman JJ, Frey ML, Harmon KM, Platt KB.

Arch Virol. 1999;144(3):525-46.


Prevalence and genetic variability of Arcobacter species in mechanically separated turkey.

Manke TR, Wesley IV, Dickson JS, Harmon KM.

J Food Prot. 1998 Dec;61(12):1623-8.


Specific identification of Campylobacter fetus by PCR targeting variable regions of the 16S rDNA.

Oyarzabal OA, Wesley IV, Harmon KM, Schroeder-Tucker L, Barbaree JM, Lauerman LH, Backert S, Conner DE.

Vet Microbiol. 1997 Oct 31;58(1):61-71.


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