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Items: 1 to 50 of 76

1.

High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium.

König L, Hartz P, Bernhardt R, Hannemann F.

Metab Eng. 2019 Sep;55:59-67. doi: 10.1016/j.ymben.2019.06.005. Epub 2019 Jun 15.

PMID:
31212031
2.

Expanding the promoter toolbox of Bacillus megaterium.

Hartz P, Mattes C, Schad M, Bernhardt R, Hannemann F.

J Biotechnol. 2019 Mar 20;294:38-48. doi: 10.1016/j.jbiotec.2019.01.018. Epub 2019 Feb 13.

PMID:
30771444
3.

Characterization and engineering of a carotenoid biosynthesis operon from Bacillus megaterium.

Hartz P, Milhim M, Trenkamp S, Bernhardt R, Hannemann F.

Metab Eng. 2018 Sep;49:47-58. doi: 10.1016/j.ymben.2018.07.017. Epub 2018 Jul 26.

PMID:
30055324
4.

CYP109E1 is a novel versatile statin and terpene oxidase from Bacillus megaterium.

Putkaradze N, Litzenburger M, Abdulmughni A, Milhim M, Brill E, Hannemann F, Bernhardt R.

Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8379-8393. doi: 10.1007/s00253-017-8552-6. Epub 2017 Oct 11.

PMID:
29018905
5.

Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium.

Abdulmughni A, Jóźwik IK, Brill E, Hannemann F, Thunnissen AWH, Bernhardt R.

FEBS J. 2017 Nov;284(22):3881-3894. doi: 10.1111/febs.14276. Epub 2017 Oct 16.

6.

Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.

Stenger B, Gerber A, Bernhardt R, Hannemann F.

Biochim Biophys Acta Proteins Proteom. 2018 Jan;1866(1):52-59. doi: 10.1016/j.bbapap.2017.08.010. Epub 2017 Sep 15.

PMID:
28870733
7.

Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase.

Abdulmughni A, Jóźwik IK, Putkaradze N, Brill E, Zapp J, Thunnissen AW, Hannemann F, Bernhardt R.

J Biotechnol. 2017 Feb 10;243:38-47. doi: 10.1016/j.jbiotec.2016.12.023. Epub 2016 Dec 30.

PMID:
28043840
8.

[Consequences of the Foundation of a University Centre for Orthopaedics and Accident Surgery].

Schneiders W, Dittmann U, Hannemann F, Jäger M, Eberlein-Gonska M, Schaser KD, Zwipp H, Günther KP.

Z Orthop Unfall. 2016 Dec;154(6):629-635. Epub 2016 Sep 9. German.

PMID:
27612316
9.

A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase.

Milhim M, Gerber A, Neunzig J, Hannemann F, Bernhardt R.

J Biotechnol. 2016 Aug 10;231:83-94. doi: 10.1016/j.jbiotec.2016.05.035. Epub 2016 May 26.

PMID:
27238232
10.

Biotransformation of the mineralocorticoid receptor antagonists spironolactone and canrenone by human CYP11B1 and CYP11B2: Characterization of the products and their influence on mineralocorticoid receptor transactivation.

Schiffer L, Müller AR, Hobler A, Brixius-Anderko S, Zapp J, Hannemann F, Bernhardt R.

J Steroid Biochem Mol Biol. 2016 Oct;163:68-76. doi: 10.1016/j.jsbmb.2016.04.004. Epub 2016 Apr 25.

PMID:
27125452
11.

An indole-deficient Escherichia coli strain improves screening of cytochromes P450 for biotechnological applications.

Brixius-Anderko S, Hannemann F, Ringle M, Khatri Y, Bernhardt R.

Biotechnol Appl Biochem. 2017 May;64(3):315-326. doi: 10.1002/bab.1488. Epub 2017 Mar 28.

PMID:
26913738
12.

Human CYP27A1 catalyzes hydroxylation of β-sitosterol and ergosterol.

Ehrhardt M, Gerber A, Zapp J, Hannemann F, Bernhardt R.

Biol Chem. 2016 Jun 1;397(6):513-8. doi: 10.1515/hsz-2016-0111.

PMID:
26891232
13.

Metabolism of Oral Turinabol by Human Steroid Hormone-Synthesizing Cytochrome P450 Enzymes.

Schiffer L, Brixius-Anderko S, Hannemann F, Zapp J, Neunzig J, Thevis M, Bernhardt R.

Drug Metab Dispos. 2016 Feb;44(2):227-37. doi: 10.1124/dmd.115.066829. Epub 2015 Dec 9.

PMID:
26658226
14.

Expression of human CYP27A1 in B. megaterium for the efficient hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol.

Ehrhardt M, Gerber A, Hannemann F, Bernhardt R.

J Biotechnol. 2016 Jan 20;218:34-40. doi: 10.1016/j.jbiotec.2015.11.021. Epub 2015 Nov 27.

PMID:
26638999
15.

Phenotypic, metabolic, and molecular genetic characterization of six patients with congenital adrenal hyperplasia caused by novel mutations in the CYP11B1 gene.

Nguyen HH, Eiden-Plach A, Hannemann F, Malunowicz EM, Hartmann MF, Wudy SA, Bernhardt R.

J Steroid Biochem Mol Biol. 2016 Jan;155(Pt A):126-34. doi: 10.1016/j.jsbmb.2015.10.011. Epub 2015 Oct 22.

PMID:
26476331
16.

A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol.

Brixius-Anderko S, Schiffer L, Hannemann F, Janocha B, Bernhardt R.

Microb Cell Fact. 2015 Sep 15;14:135. doi: 10.1186/s12934-015-0333-2.

17.

Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.

Gerber A, Kleser M, Biedendieck R, Bernhardt R, Hannemann F.

Microb Cell Fact. 2015 Jul 29;14:107. doi: 10.1186/s12934-015-0300-y.

18.

Regioselective Acetylation of C21 Hydroxysteroids by the Bacterial Chloramphenicol Acetyltransferase I.

Mosa A, Hutter MC, Zapp J, Bernhardt R, Hannemann F.

Chembiochem. 2015 Jul 27;16(11):1670-9. doi: 10.1002/cbic.201500125. Epub 2015 Jun 12.

PMID:
25999128
19.

A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale.

Schiffer L, Anderko S, Hobler A, Hannemann F, Kagawa N, Bernhardt R.

Microb Cell Fact. 2015 Feb 25;14:25. doi: 10.1186/s12934-015-0209-5.

20.

2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation.

Mosa A, Neunzig J, Gerber A, Zapp J, Hannemann F, Pilak P, Bernhardt R.

J Steroid Biochem Mol Biol. 2015 Jun;150:1-10. doi: 10.1016/j.jsbmb.2015.02.014. Epub 2015 Mar 3.

PMID:
25746800
21.

Characterization of the gene cluster CYP264B1-geoA from Sorangium cellulosum So ce56: biosynthesis of (+)-eremophilene and its hydroxylation.

Schifrin A, Ly TT, Günnewich N, Zapp J, Thiel V, Schulz S, Hannemann F, Khatri Y, Bernhardt R.

Chembiochem. 2015 Jan 19;16(2):337-44. doi: 10.1002/cbic.201402443. Epub 2014 Dec 12.

PMID:
25504914
22.

The CYP11B subfamily.

Schiffer L, Anderko S, Hannemann F, Eiden-Plach A, Bernhardt R.

J Steroid Biochem Mol Biol. 2015 Jul;151:38-51. doi: 10.1016/j.jsbmb.2014.10.011. Epub 2014 Oct 18. Review.

PMID:
25465475
23.

A natural heme-signature variant of CYP267A1 from Sorangium cellulosum So ce56 executes diverse ω-hydroxylation.

Khatri Y, Hannemann F, Girhard M, Kappl R, Hutter M, Urlacher VB, Bernhardt R.

FEBS J. 2015 Jan;282(1):74-88. doi: 10.1111/febs.13104. Epub 2014 Nov 4.

24.

[Complications of metal-on-metal tribological pairing].

Stiehler M, Zobel F, Hannemann F, Schmitt J, Lützner J, Kirschner S, Günther KP, Hartmann A.

Orthopade. 2014 Jan;43(1):79-91. doi: 10.1007/s00132-013-2131-z. German.

PMID:
24356820
25.

Metal ion concentrations in body fluids after implantation of hip replacements with metal-on-metal bearing--systematic review of clinical and epidemiological studies.

Hartmann A, Hannemann F, Lützner J, Seidler A, Drexler H, Günther KP, Schmitt J.

PLoS One. 2013 Aug 7;8(8):e70359. doi: 10.1371/journal.pone.0070359. eCollection 2013. Review.

26.

A new cytochrome P450 system from Bacillus megaterium DSM319 for the hydroxylation of 11-keto-β-boswellic acid (KBA).

Brill E, Hannemann F, Zapp J, Brüning G, Jauch J, Bernhardt R.

Appl Microbiol Biotechnol. 2014 Feb;98(4):1701-17. Epub 2013 Jun 25.

PMID:
23797329
27.

[Update on metal-on-metal hip joints].

Günther KP, Lützner J, Hannemann F, Schmitt J, Kirschner S, Goronzy J, Stiehler M, Lohmann C, Hartmann A.

Orthopade. 2013 May;42(5):373-87; quiz 388-9. doi: 10.1007/s00132-013-2100-6. German.

PMID:
23632650
28.

Novel family members of CYP109 from Sorangium cellulosum So ce56 exhibit characteristic biochemical and biophysical properties.

Khatri Y, Hannemann F, Girhard M, Kappl R, Même A, Ringle M, Janocha S, Leize-Wagner E, Urlacher VB, Bernhardt R.

Biotechnol Appl Biochem. 2013 Jan-Feb;60(1):18-29. doi: 10.1002/bab.1087.

PMID:
23586989
29.

European multidisciplinary consensus statement on the use and monitoring of metal-on-metal bearings for total hip replacement and hip resurfacing.

Hannemann F, Hartmann A, Schmitt J, Lützner J, Seidler A, Campbell P, Delaunay CP, Drexler H, Ettema HB, García-Cimbrelo E, Huberti H, Knahr K, Kunze J, Langton DJ, Lauer W, Learmonth I, Lohmann CH, Morlock M, Wimmer MA, Zagra L, Günther KP.

Orthop Traumatol Surg Res. 2013 May;99(3):263-71. doi: 10.1016/j.otsr.2013.01.005. Epub 2013 Mar 16.

30.

Consensus statement "Current evidence on the management of metal-on-metal bearings"--April 16, 2012.

Günther KP, Schmitt J, Campbell P, Delaunay CP, Drexler H, Ettema HB, García-Cimbrelo E, Hannemann F, Hartmann A, Huberti H, Knahr K, Kunze J, Langton DJ, Lauer W, Learmonth I, Lohmann CH, Lützner J, Morlock M, Seidler A, Wimmer MA, Zagra L.

Hip Int. 2013 Jan-Feb;23(1):2-5. doi: 10.5301/HIP.2013.10754. No abstract available.

PMID:
23417528
31.

Application of a new versatile electron transfer system for cytochrome P450-based Escherichia coli whole-cell bioconversions.

Ringle M, Khatri Y, Zapp J, Hannemann F, Bernhardt R.

Appl Microbiol Biotechnol. 2013 Sep;97(17):7741-54. doi: 10.1007/s00253-012-4612-0. Epub 2012 Dec 20.

PMID:
23254762
32.

Changing the regioselectivity of a P450 from C15 to C11 hydroxylation of progesterone.

Nguyen KT, Virus C, Günnewich N, Hannemann F, Bernhardt R.

Chembiochem. 2012 May 29;13(8):1161-6. doi: 10.1002/cbic.201100811. Epub 2012 Apr 24.

PMID:
22532270
33.

Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level.

Hobler A, Kagawa N, Hutter MC, Hartmann MF, Wudy SA, Hannemann F, Bernhardt R.

J Steroid Biochem Mol Biol. 2012 Oct;132(1-2):57-65. doi: 10.1016/j.jsbmb.2012.03.002. Epub 2012 Mar 16.

PMID:
22446688
34.

Autodisplay of functional CYP106A2 in Escherichia coli.

Schumacher SD, Hannemann F, Teese MG, Bernhardt R, Jose J.

J Biotechnol. 2012 Oct 15;161(2):104-12. doi: 10.1016/j.jbiotec.2012.02.018. Epub 2012 Mar 8.

PMID:
22426093
35.

Direct and mediated electrochemical response of the cytochrome P450 106A2 from Bacillus megaterium ATCC 13368.

Colas H, Ewen KM, Hannemann F, Bistolas N, Wollenberger U, Bernhardt R, de Oliveira P.

Bioelectrochemistry. 2012 Oct;87:71-7. doi: 10.1016/j.bioelechem.2012.01.006. Epub 2012 Jan 30.

PMID:
22342191
36.

Alu Sx repeat-induced homozygous deletion of the StAR gene causes lipoid congenital adrenal hyperplasia.

Eiden-Plach A, Nguyen HH, Schneider U, Hartmann MF, Bernhardt R, Hannemann F, Wudy SA.

J Steroid Biochem Mol Biol. 2012 May;130(1-2):1-6. doi: 10.1016/j.jsbmb.2011.12.016. Epub 2012 Jan 11.

PMID:
22249004
37.

CYP105A1 mediated 3-hydroxylation of glimepiride and glibenclamide using a recombinant Bacillus megaterium whole-cell catalyst.

Kleser M, Hannemann F, Hutter M, Zapp J, Bernhardt R.

J Biotechnol. 2012 Feb 10;157(3):405-12. doi: 10.1016/j.jbiotec.2011.12.006. Epub 2011 Dec 21.

PMID:
22202177
38.

Functional characterization of Fdx1: evidence for an evolutionary relationship between P450-type and ISC-type ferredoxins.

Ewen KM, Hannemann F, Iametti S, Morleo A, Bernhardt R.

J Mol Biol. 2011 Nov 11;413(5):940-51. doi: 10.1016/j.jmb.2011.09.010. Epub 2011 Sep 16.

PMID:
21945528
39.

A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-β-boswellic acid (KBA) based on a recombinant cytochrome P450 system.

Bleif S, Hannemann F, Zapp J, Hartmann D, Jauch J, Bernhardt R.

Appl Microbiol Biotechnol. 2012 Feb;93(3):1135-46. doi: 10.1007/s00253-011-3467-0. Epub 2011 Jul 22.

PMID:
21779845
40.

Structural and thermodynamic characterization of the adrenodoxin-like domain of the electron-transfer protein Etp1 from Schizosaccharomyces pombe.

Müller JJ, Hannemann F, Schiffler B, Ewen KM, Kappl R, Heinemann U, Bernhardt R.

J Inorg Biochem. 2011 Jul;105(7):957-65. doi: 10.1016/j.jinorgbio.2011.04.001. Epub 2011 Apr 9.

PMID:
21536008
41.

Investigation of cytochromes P450 in myxobacteria: excavation of cytochromes P450 from the genome of Sorangium cellulosum So ce56.

Khatri Y, Hannemann F, Perlova O, Müller R, Bernhardt R.

FEBS Lett. 2011 Jun 6;585(11):1506-13. doi: 10.1016/j.febslet.2011.04.035. Epub 2011 Apr 22. Review.

42.

Identification of CYP106A2 as a regioselective allylic bacterial diterpene hydroxylase.

Bleif S, Hannemann F, Lisurek M, von Kries JP, Zapp J, Dietzen M, Antes I, Bernhardt R.

Chembiochem. 2011 Mar 7;12(4):576-82. doi: 10.1002/cbic.201000404. Epub 2011 Jan 26.

PMID:
21271628
43.

The CYPome of Sorangium cellulosum So ce56 and identification of CYP109D1 as a new fatty acid hydroxylase.

Khatri Y, Hannemann F, Ewen KM, Pistorius D, Perlova O, Kagawa N, Brachmann AO, Müller R, Bernhardt R.

Chem Biol. 2010 Dec 22;17(12):1295-305. doi: 10.1016/j.chembiol.2010.10.010.

44.

A solution model of the complex formed by adrenodoxin and adrenodoxin reductase determined by paramagnetic NMR spectroscopy.

Keizers PH, Mersinli B, Reinle W, Donauer J, Hiruma Y, Hannemann F, Overhand M, Bernhardt R, Ubbink M.

Biochemistry. 2010 Aug 17;49(32):6846-55. doi: 10.1021/bi100598f.

PMID:
20695524
45.

Regioselective hydroxylation of norisoprenoids by CYP109D1 from Sorangium cellulosum So ce56.

Khatri Y, Girhard M, Romankiewicz A, Ringle M, Hannemann F, Urlacher VB, Hutter MC, Bernhardt R.

Appl Microbiol Biotechnol. 2010 Sep;88(2):485-95. doi: 10.1007/s00253-010-2756-3. Epub 2010 Jul 20.

PMID:
20645086
46.

Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesis.

Sheftel AD, Stehling O, Pierik AJ, Elsässer HP, Mühlenhoff U, Webert H, Hobler A, Hannemann F, Bernhardt R, Lill R.

Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11775-80. doi: 10.1073/pnas.1004250107. Epub 2010 Jun 14.

47.

Five novel mutations in CYP11B2 gene detected in patients with aldosterone synthase deficiency type I: Functional characterization and structural analyses.

Nguyen HH, Hannemann F, Hartmann MF, Malunowicz EM, Wudy SA, Bernhardt R.

Mol Genet Metab. 2010 Aug;100(4):357-64. doi: 10.1016/j.ymgme.2010.04.016. Epub 2010 May 21.

PMID:
20494601
48.

Towards preparative scale steroid hydroxylation with cytochrome P450 monooxygenase CYP106A2.

Zehentgruber D, Hannemann F, Bleif S, Bernhardt R, Lütz S.

Chembiochem. 2010 Mar 22;11(5):713-21. doi: 10.1002/cbic.200900706.

PMID:
20183841
49.

Genome mining in Sorangium cellulosum So ce56: identification and characterization of the homologous electron transfer proteins of a myxobacterial cytochrome P450.

Ewen KM, Hannemann F, Khatri Y, Perlova O, Kappl R, Krug D, Hüttermann J, Müller R, Bernhardt R.

J Biol Chem. 2009 Oct 16;284(42):28590-8. doi: 10.1074/jbc.M109.021717. Epub 2009 Aug 20.

50.

Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.

Goñi G, Zöllner A, Lisurek M, Velázquez-Campoy A, Pinto S, Gómez-Moreno C, Hannemann F, Bernhardt R, Medina M.

Biochim Biophys Acta. 2009 Nov;1794(11):1635-42. doi: 10.1016/j.bbapap.2009.07.012. Epub 2009 Jul 25.

PMID:
19635596

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