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Items: 1 to 50 of 71

1.

Import of extracellular ATP in yeast and man modulates AMPK and TORC1 signalling.

Forte GM, Davie E, Lie S, Franz-Wachtel M, Ovens AJ, Wang T, Oakhill JS, Maček B, Hagan IM, Petersen J.

J Cell Sci. 2019 Apr 3;132(7). pii: jcs223925. doi: 10.1242/jcs.223925.

2.

Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment.

Chan KY, Alonso-Nuñez M, Grallert A, Tanaka K, Connolly Y, Smith DL, Hagan IM.

J Cell Biol. 2017 Sep 4;216(9):2795-2812. doi: 10.1083/jcb.201702172. Epub 2017 Aug 3.

3.

Elementary Protein Analysis in Schizosaccharomyces pombe.

Grallert A, Hagan IM.

Cold Spring Harb Protoc. 2017 Mar 1;2017(3). doi: 10.1101/pdb.top079806.

PMID:
28250233
4.

Large-Scale Immunoprecipitation from Fission Yeast Cell Extracts.

Grallert A, Hagan IM.

Cold Spring Harb Protoc. 2017 Feb 1;2017(2). doi: 10.1101/pdb.prot091595.

PMID:
28148853
5.

Small-Scale Immunoprecipitation from Fission Yeast Cell Extracts.

Grallert A, Hagan IM.

Cold Spring Harb Protoc. 2017 Feb 1;2017(2). doi: 10.1101/pdb.prot091587.

PMID:
28148852
6.

Preparation of Protein Extracts from Schizosaccharomyces pombe Using Trichloroacetic Acid Precipitation.

Grallert A, Hagan IM.

Cold Spring Harb Protoc. 2017 Feb 1;2017(2). doi: 10.1101/pdb.prot091579.

PMID:
28148851
7.

Analysis of the Schizosaccharomyces pombe Cell Cycle.

Hagan IM, Grallert A, Simanis V.

Cold Spring Harb Protoc. 2016 Sep 1;2016(9). doi: 10.1101/pdb.top082800.

PMID:
27587785
8.

Synchronizing Progression of Schizosaccharomyces pombe Cells from G2 through Repeated Rounds of Mitosis and S Phase with cdc25-22 Arrest Release.

Hagan IM, Grallert A, Simanis V.

Cold Spring Harb Protoc. 2016 Aug 1;2016(8). doi: 10.1101/pdb.prot091264.

PMID:
27480720
9.

Synchronizing Progression of Schizosaccharomyces pombe Cells from Prophase through Mitosis and into S Phase with nda3-KM311 Arrest Release.

Hagan IM, Grallert A, Simanis V.

Cold Spring Harb Protoc. 2016 Aug 1;2016(8). doi: 10.1101/pdb.prot091256.

PMID:
27480719
10.

Fixed-Cell Imaging of Schizosaccharomyces pombe.

Hagan IM, Bagley S.

Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.top079830.

PMID:
27371603
11.

Immunofluorescence Microscopy of Schizosaccharomyces pombe Using Chemical Fixation.

Hagan IM.

Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.prot091017.

PMID:
27371599
12.

Cell Cycle Synchronization of Schizosaccharomyces pombe by Lactose Gradient Centrifugation to Isolate Small Cells.

Hagan IM, Grallert A, Simanis V.

Cold Spring Harb Protoc. 2016 Jun 1;2016(6). doi: 10.1101/pdb.prot091249.

PMID:
27250945
13.

Cell Cycle Synchronization of Schizosaccharomyces pombe by Centrifugal Elutriation of Small Cells.

Hagan IM, Grallert A, Simanis V.

Cold Spring Harb Protoc. 2016 Jun 1;2016(6). doi: 10.1101/pdb.prot091231.

PMID:
27250944
14.

Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates.

Hagan IM.

Cold Spring Harb Protoc. 2016 Jun 1;2016(6). doi: 10.1101/pdb.prot091033.

PMID:
27250943
15.

Chromatin and Cell Wall Staining of Schizosaccharomyces pombe.

Hagan IM.

Cold Spring Harb Protoc. 2016 Jun 1;2016(6). doi: 10.1101/pdb.prot091025.

PMID:
27250942
16.

The centrosome and its duplication cycle.

Fu J, Hagan IM, Glover DM.

Cold Spring Harb Perspect Biol. 2015 Feb 2;7(2):a015800. doi: 10.1101/cshperspect.a015800. Review.

17.

A PP1-PP2A phosphatase relay controls mitotic progression.

Grallert A, Boke E, Hagting A, Hodgson B, Connolly Y, Griffiths JR, Smith DL, Pines J, Hagan IM.

Nature. 2015 Jan 1;517(7532):94-98. doi: 10.1038/nature14019. Epub 2014 Dec 10.

18.

Nic1 inactivation enables stable isotope labeling with 13C615N4-arginine in Schizosaccharomyces pombe.

Carpy A, Patel A, Tay YD, Hagan IM, Macek B.

Mol Cell Proteomics. 2015 Jan;14(1):243-50. doi: 10.1074/mcp.O114.045302. Epub 2014 Nov 3.

19.

Extending the Schizosaccharomyces pombe molecular genetic toolbox.

Fennessy D, Grallert A, Krapp A, Cokoja A, Bridge AJ, Petersen J, Patel A, Tallada VA, Boke E, Hodgson B, Simanis V, Hagan IM.

PLoS One. 2014 May 21;9(5):e97683. doi: 10.1371/journal.pone.0097683. eCollection 2014.

20.

Spatial control of mitotic commitment in fission yeast.

Hagan IM, Grallert A.

Biochem Soc Trans. 2013 Dec;41(6):1766-71. doi: 10.1042/BST20130190. Review.

PMID:
24256289
21.

Mutation of a conserved residue enhances the sensitivity of analogue-sensitised kinases to generate a novel approach to the study of mitosis in fission yeast.

Tay YD, Patel A, Kaemena DF, Hagan IM.

J Cell Sci. 2013 Nov 1;126(Pt 21):5052-61. doi: 10.1242/jcs.135301. Epub 2013 Aug 28.

22.

Removal of centrosomal PP1 by NIMA kinase unlocks the MPF feedback loop to promote mitotic commitment in S. pombe.

Grallert A, Chan KY, Alonso-Nuñez ML, Madrid M, Biswas A, Alvarez-Tabarés I, Connolly Y, Tanaka K, Robertson A, Ortiz JM, Smith DL, Hagan IM.

Curr Biol. 2013 Feb 4;23(3):213-22. doi: 10.1016/j.cub.2012.12.039. Epub 2013 Jan 17.

23.

Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast.

Grallert A, Patel A, Tallada VA, Chan KY, Bagley S, Krapp A, Simanis V, Hagan IM.

Nat Cell Biol. 2013 Jan;15(1):88-95. doi: 10.1038/ncb2633. Epub 2012 Dec 9.

24.

The S. pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1.

Grallert A, Connolly Y, Smith DL, Simanis V, Hagan IM.

Nat Cell Biol. 2012 Jun 10;14(7):738-45. doi: 10.1038/ncb2514.

25.

Programmed fluctuations in sense/antisense transcript ratios drive sexual differentiation in S. pombe.

Bitton DA, Grallert A, Scutt PJ, Yates T, Li Y, Bradford JR, Hey Y, Pepper SD, Hagan IM, Miller CJ.

Mol Syst Biol. 2011 Dec 20;7:559. doi: 10.1038/msb.2011.90.

26.

Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle.

Tamm T, Grallert A, Grossman EP, Alvarez-Tabares I, Stevens FE, Hagan IM.

J Cell Biol. 2011 Oct 31;195(3):467-84. doi: 10.1083/jcb.201106076.

27.

Polo, greatwall, and protein phosphatase PP2A Jostle for pole position.

Boke E, Hagan IM.

PLoS Genet. 2011 Aug;7(8):e1002213. doi: 10.1371/journal.pgen.1002213. Epub 2011 Aug 11. No abstract available.

28.

Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability.

Bitton DA, Wood V, Scutt PJ, Grallert A, Yates T, Smith DL, Hagan IM, Miller CJ.

Genetics. 2011 Apr;187(4):1207-17. doi: 10.1534/genetics.110.123497. Epub 2011 Jan 26.

29.

The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope.

Tallada VA, Tanaka K, Yanagida M, Hagan IM.

J Cell Biol. 2009 Jun 1;185(5):875-88. doi: 10.1083/jcb.200812108.

30.

Stress-regulated kinase pathways in the recovery of tip growth and microtubule dynamics following osmotic stress in S. pombe.

Robertson AM, Hagan IM.

J Cell Sci. 2008 Dec 15;121(Pt 24):4055-68. doi: 10.1242/jcs.034488. Epub 2008 Nov 25.

31.

The spindle pole body plays a key role in controlling mitotic commitment in the fission yeast Schizosaccharomyces pombe.

Hagan IM.

Biochem Soc Trans. 2008 Oct;36(Pt 5):1097-101. doi: 10.1042/BST0361097. Review.

PMID:
18793196
32.

Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth.

Alvarez-Tabarés I, Grallert A, Ortiz JM, Hagan IM.

J Cell Sci. 2007 Oct 15;120(Pt 20):3589-601. Epub 2007 Sep 25.

33.
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35.

Warming up at the poles.

Hagan IM, Palazzo RE.

EMBO Rep. 2006 Apr;7(4):364-71. Epub 2006 Mar 24. No abstract available.

36.

Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivity.

Unwin RD, Griffiths JR, Leverentz MK, Grallert A, Hagan IM, Whetton AD.

Mol Cell Proteomics. 2005 Aug;4(8):1134-44. Epub 2005 May 27.

37.

Polo kinase links the stress pathway to cell cycle control and tip growth in fission yeast.

Petersen J, Hagan IM.

Nature. 2005 May 26;435(7041):507-12.

PMID:
15917811
38.

The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer.

Hayward DG, Clarke RB, Faragher AJ, Pillai MR, Hagan IM, Fry AM.

Cancer Res. 2004 Oct 15;64(20):7370-6.

40.
41.
43.

Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.

Anderson M, Ng SS, Marchesi V, MacIver FH, Stevens FE, Riddell T, Glover DM, Hagan IM, McInerny CJ.

EMBO J. 2002 Nov 1;21(21):5745-55.

45.

MTOC formation during mitotic exit in fission yeast.

Heitz MJ, Petersen J, Valovin S, Hagan IM.

J Cell Sci. 2001 Dec;114(Pt 24):4521-32.

46.
47.

New insights into development from mitosis of a unicellular yeast.

Hagan IM, Jones N, Carr AM.

Dev Cell. 2001 Aug;1(2):158-60. Review.

48.

The mitotic roles of Polo-like kinase.

Donaldson MM, Tavares AA, Hagan IM, Nigg EA, Glover DM.

J Cell Sci. 2001 Jul;114(Pt 13):2357-8. No abstract available.

49.

The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe.

Tanaka K, Petersen J, MacIver F, Mulvihill DP, Glover DM, Hagan IM.

EMBO J. 2001 Mar 15;20(6):1259-70.

50.

The microtubule organizing centers of Schizosaccharomyces pombe.

Hagan IM, Petersen J.

Curr Top Dev Biol. 2000;49:133-59. Review. No abstract available.

PMID:
11005017

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