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Sci Signal. 2020 Feb 25;13(620). pii: eaax9940. doi: 10.1126/scisignal.aax9940.

Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.

Author information

1
Department of Biological Sciences, University of Cyprus, University Avenue 1, New Campus, Nicosia 2109, Cyprus.
2
Department of Biological Sciences, University of Cyprus, University Avenue 1, New Campus, Nicosia 2109, Cyprus. skourip@ucy.ac.cy.

Abstract

Correct selection of the cell division axis is important for cell differentiation, tissue and organ morphogenesis, and homeostasis. Both integrins, which mediate interactions with extracellular matrix (ECM) components such as fibronectin, and cadherins, which mediate interactions between cells, are implicated in the determination of spindle orientation. We found that both cadherin- and integrin-based adhesion resulted in cell divisions parallel to the attachment plane and elicited identical spindle responses to spatial adhesive cues. This suggests that adhesion topology provides purely mechanical spatial cues that are independent of the molecular nature of the interaction or signaling from adhesion complexes. We also demonstrated that cortical integrin activation was indispensable for correct spindle orientation on both cadherin and fibronectin substrates. These data suggest that spindle orientation responses to adhesion topology are primarily a result of force anisotropy on the cell cortex and show that integrins play a central role in this process that is distinct from their role in cell-ECM interactions.

PMID:
32098802
DOI:
10.1126/scisignal.aax9940

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